anticodon and 1-10-phenanthroline

anticodon has been researched along with 1-10-phenanthroline* in 1 studies

Other Studies

1 other study(ies) available for anticodon and 1-10-phenanthroline

Article	Year
Determination of nucleotide distances in RNA by means of copper phenanthroline-generated hydroxyl radical cleavage pattern. RNA (New York, N.Y.), 1995, Volume: 1, Issue:10 In contrast to the commonly used Fe(II)-EDTA, bis(orthophenanthroline)-copper(I) (OP-Cu) first generates hydroxyl radicals after binding to RNA. Due to diffusion, the hydroxyl radicals can cleave neighboring nucleotides in a distance r of up to 1.5 nm to the OP-Cu binding site. Using the known structure of tRNAPhe as a reference, we show that the hydroxyl radical cleavage pattern generated by a specifically bound OP-Cu shows a 1/r dependence on the distance of the cleaved nucleotide to the OP-Cu binding site. We propose that OP-Cu is a suitable probe for obtaining data on the distances between nucleotides in RNA, which can be used in modeling the structure of the examined RNA. However, this information is restricted to about three to four bases surrounding an OP-Cu binding site. Topics: Anticodon; Binding Sites; Hydroxyl Radical; Models, Molecular; Molecular Probes; Molecular Structure; Nucleic Acid Conformation; Nucleotides; Phenanthrolines; RNA; RNA, Fungal; RNA, Transfer, Phe; Saccharomyces cerevisiae; Thermodynamics	1995

Article

Year

Determination of nucleotide distances in RNA by means of copper phenanthroline-generated hydroxyl radical cleavage pattern.

RNA (New York, N.Y.), 1995, Volume: 1, Issue:10

In contrast to the commonly used Fe(II)-EDTA, bis(orthophenanthroline)-copper(I) (OP-Cu) first generates hydroxyl radicals after binding to RNA. Due to diffusion, the hydroxyl radicals can cleave neighboring nucleotides in a distance r of up to 1.5 nm to the OP-Cu binding site. Using the known structure of tRNAPhe as a reference, we show that the hydroxyl radical cleavage pattern generated by a specifically bound OP-Cu shows a 1/r dependence on the distance of the cleaved nucleotide to the OP-Cu binding site. We propose that OP-Cu is a suitable probe for obtaining data on the distances between nucleotides in RNA, which can be used in modeling the structure of the examined RNA. However, this information is restricted to about three to four bases surrounding an OP-Cu binding site.

Topics: Anticodon; Binding Sites; Hydroxyl Radical; Models, Molecular; Molecular Probes; Molecular Structure; Nucleic Acid Conformation; Nucleotides; Phenanthrolines; RNA; RNA, Fungal; RNA, Transfer, Phe; Saccharomyces cerevisiae; Thermodynamics

1995