antibiotic g 418 has been researched along with gentamicin in 488 studies
| Studies (antibiotic g 418) | Trials (antibiotic g 418) | Recent Studies (post-2010) (antibiotic g 418) | Studies (gentamicin) | Trials (gentamicin) | Recent Studies (post-2010) (gentamicin) |
|---|---|---|---|---|---|
| 515 | 0 | 102 | 19,191 | 1,365 | 3,650 |
| Timeframe | Studies, this research(%) | All Research% |
|---|---|---|
| pre-1990 | 63 (12.91) | 18.7374 |
| 1990's | 187 (38.32) | 18.2507 |
| 2000's | 133 (27.25) | 29.6817 |
| 2010's | 90 (18.44) | 24.3611 |
| 2020's | 15 (3.07) | 2.80 |
| Authors | Studies |
|---|---|
| Beck, H; Sack, K | 1 |
| Olson, LD; Rodabaugh, DE | 1 |
| Counels, M; Loebenberg, D; Waitz, JA | 1 |
| Caplan, A; Dekeyser, R; Van Montagu, M | 1 |
| Ishidate, M; Mizusawa, H; Sasaki, K; Tanaka, N | 1 |
| Mudrak, I; Ogris, E; Wintersberger, E | 1 |
| Randall, TA; Reddy, CA | 1 |
| Bartholomew, C; Brenner, MK; Buschle, M; Foreman, NK; Ihle, JN; Krance, RA; Mirro, J; Moen, RC; Rill, DR | 1 |
| Gotoh, N; Hino, M; Shibuya, M; Tojo, A; Yazaki, Y | 1 |
| Bird, RC; Pai, SR | 1 |
| Kesbeke, F; Knetsch, ML; Ludérus, ME; Reymond, CD; Snaar-Jagalska, BE; Van Driel, R | 1 |
| Arimura, H; Ishida, Y; Kawabe, H; Masaki, A; Motai, H; Murakami, K; Murakami, S; Nakano, E; Ogawa, Y; Tatsumi, H | 1 |
| Legendre, D; Rommelaere, J | 1 |
| Bartholomew, C; Brenner, MK; Buschle, M; Foreman, NK; Ihle, JN; Moen, RC; Rill, DR; Santana, VM | 1 |
| Belmont, J; Brenner, M; Claxton, D; Ellerson, D; Etkin, M; Filaccio, M; Gaozza, E; Moen, R; Moore, KA; Suh, SP | 1 |
| Bevan, MW; Chilton, MD; Flavell, RB | 1 |
| Mercer, WE; Noble, JR; Reddel, RR; Willetts, KE | 1 |
| Baker, RM; Dolnick, BJ; Hanchett, LA; Meegan, RL; Wang, SJ | 1 |
| Arcucci, A; De Lorenzo, F; Mallardo, M; Quinto, I; Ruocco, MR; Scala, G | 1 |
| Allen, LA; Raetz, CR | 1 |
| Capecchi, MR; Deng, C; Thomas, KR | 1 |
| Miller, AD; Osborne, WR; Stockschlaeder, MA; Storb, R | 1 |
| Alt, M; Fleckenstein, B; Grassmann, R | 1 |
| Alt, M; Berthold, S; Buckreus, I; Fleckenstein, B; Grassmann, R; Platzer, E; Simmer, B | 1 |
| Chen, XJ; Fukuhara, H; Tanguy-Rougeau, C; Wésolowski-Louvel, M | 1 |
| Miyazaki, J; Niwa, H; Yamamura, K | 1 |
| Hofer, KG; Schneiderman, GS; Schneiderman, MH | 1 |
| Cerni, C; Meneguzzi, G; Patocka, K | 1 |
| De Benedetti, A; Rhoads, RE | 1 |
| Kim, H; Kim, S; Salazar-Grueso, EF | 1 |
| Philippsen, P; Wright, MC | 1 |
| Koizumi, S | 1 |
| Martinelli, SD; Sheikh, A | 1 |
| Aebersold, P; Kasid, A; Rosenberg, SA | 1 |
| Able, C; Anderson, WF; Blaese, RM; Cornetta, K; Culver, KW; Lee, RT; Lenschow, D; Morgan, RA; Osborne, WR | 1 |
| Dougherty, JP; Temin, HM | 1 |
| Aebersold, P; Anderson, WF; Blaese, RM; Cornetta, K; Karson, E; Kasid, A; Morecki, S; Rosenberg, SA | 1 |
| Aebersold, P; Anderson, WF; Blaese, RM; Cornetta, K; Culver, K; Kasid, A; Lotze, M; Morecki, S; Morgan, R; Rosenberg, SA | 1 |
| Gaben, AM; Mester, J | 1 |
| Diatloff-Zito, C; Fraser, D; Moustacchi, E | 1 |
| Marty, L; Piechaczyk, M; Roux, P; Royer, M | 1 |
| Bhattacharyya, NP; Maher, VM; McCormick, JJ | 2 |
| Jacobberger, JW; Sladek, TL | 1 |
| Brown, R; Mee, PJ | 1 |
| Anderson, WF; Cornetta, K; Forough, R; Freeman, SM; Maciag, T; Zwiebel, JA | 1 |
| Fisher, JH; McCormack, FX; Shannon, JM; Smith, DL; Suwabe, A; Voelker, DR | 1 |
| Burak, E; Hadfield, C; Jordan, BE; Mount, RC; Pretorius, GH | 1 |
| Lee, MG; Van der Ploeg, LH | 1 |
| Bostock, CJ; Harwood, AJ; Shervington, A | 1 |
| Hayasaka, K; Mitani, H; Sato, M; Shima, A | 1 |
| Caskey, CT; Stout, JT | 1 |
| Hellgren, D; Lambert, B; Sahlén, S | 1 |
| Flowers, ME; Hackman, R; Miller, AD; Niederwieser, D; Schuening, FG; Stockschlaeder, MA; Storb, R | 1 |
| Anichini, A; Dellabona, P; DeMonte, LB; Malavasi, F; Mariani, M; Momo, M; Natali, PG; Nistico, P; Tecce, R | 1 |
| Chang, CC; De Feijter, AW; Goodman, JI; Klaunig, JE; Ray, JS; Ruch, RJ; Trosko, JE; Weghorst, CM | 1 |
| Anderson, WF; Crumbleholme, T; Ekhterae, D; Harrison, MR; Karson, E; Zanjani, ED | 1 |
| Brzeski, H; Kinne, R; MacDonald, C; Scott, DM | 1 |
| Hellgren, D; Lambert, B; Luthman, H | 1 |
| Gaido, ML; Strobl, JS | 1 |
| Butel, JS; Danielson, KG; Durban, EM; Kittrell, FS; Knepper, JE; Medina, D | 1 |
| Marek, ET; Schardl, CL; Smith, DA | 1 |
| Gmünder, H; Kohli, J | 1 |
| Duch, M; Jørgensen, P; Kjeldgaard, NO; Paludan, K; Pedersen, FS | 1 |
| Gerwin, BI; Harris, CC; Malan-Shibley, L; Metcalf, RA; Reddel, RR | 1 |
| Adamson, ED; Edwards, SA | 1 |
| Hayashi, S; Katoh, K; Kondoh, H; Takahashi, Y | 1 |
| Anderson, WF; Eglitis, MA; Jolly, JD; Jones, JB; Kantoff, PW; Lothrop, CD | 1 |
| Heidmann, O; Heidmann, T; Nicolas, JF | 1 |
| Carter, BJ; Mendelson, E; Smith, MG | 1 |
| Dubey, DP; Faller, DV; Koka, P; Passarelli, AL; Ynis, EJ; Yunis, J | 1 |
| Gupta, D; Tartakoff, A; Tisdale, E | 1 |
| Carman, MD; Gamba-Vitalo, C; Sartorelli, AC | 1 |
| Burke, JF; Mogg, AE | 1 |
| Echalier, G; Maisonhaute, C | 1 |
| Riggio, M; Spandidos, DA | 1 |
| Bergholz, U; Kollek, R; Ostertag, W; Seliger, B; Smadja-Joffe, F; Stocking, C | 1 |
| Niwa, O | 1 |
| Franke, CA; Hruby, DE; Rice, CM; Strauss, JH | 1 |
| Friel, J; Ostertag, W; Stacey, A; Stocking, C | 1 |
| Coon, HG; Gwadz, RW; Kantoff, P; Miller, LH; Romans, P; Sakai, RK | 1 |
| Crouzet, M; Grant, CM; Izgu, F; Tuite, MF | 1 |
| Johnson, GR | 1 |
| Collins, SJ | 1 |
| Beaty, BJ; Carlson, JO; McGrane, V; Miller, BR | 1 |
| Hasnain, SE; Leung, WC; Manavathu, EK; Suryanarayana, K | 1 |
| Bernstein, A; Dick, JE; Huszar, D; Phillips, RA; Wu, DD | 1 |
| Chang, W; Dick, JE; Fauser, AA; Kamel-Reid, S; Laneuville, P | 1 |
| Hayes, H; Kaneda, Y; Okada, Y; Uchida, T | 1 |
| Darlington, GJ; Hahn, T; Ledley, FD; Woo, SL | 1 |
| Ayares, D; Chekuri, L; Kucherlapati, R; Song, KY | 1 |
| Hartwig, R; Schweiger, HG; Schweiger, M | 1 |
| Jayaram, M; Revuelta, JL | 1 |
| Fallon, AM; Nouri, N | 1 |
| Buchanan, JH; Sidhu, J; Stevens, A | 1 |
| Maher, VM; McCormick, JJ; Morgan, TL | 1 |
| Buchanan, JH; Sidhu, J | 1 |
| Angenent, GC; Krump-Konvalinkova, V | 1 |
| Palacios, R; Steinmetz, M | 1 |
| de Crombrugghe, B; Liau, G; Setoyama, C | 1 |
| Brady, G; Brown, R; Funk, A; Mattern, J; Schütz, G | 1 |
| Baker, F; Black, HE; Frantz, JD; La Rocca, PT; Schwartz, E; Szot, RJ | 1 |
| Wagman, GH; Weinstein, MJ | 1 |
| Bar-Nun, S; Beckmann, JS; Shneyour, Y | 1 |
| Colbère-Garapin, F; Garapin, AC; Horodniceanu, F; Kourilsky, P | 1 |
| Byrne, JC; Howley, PM; Law, MF | 1 |
| Barclay, SL; Meller, E | 1 |
| Dickson, RC; Webster, TD | 1 |
| Blam, SB; Kaplan, HS; Riera, FC; Teng, NN | 1 |
| Panchal, CJ; Stewart, GG; Whitney, GK | 1 |
| Chenevert, JM; Dumas, LB; Friedberg, EC; Naumovski, L | 1 |
| Holzhausen, H; Kiessling, U; Schöneich, J; Strauss, M; Wobus, AM | 1 |
| Alfano, FD; Halaban, R | 1 |
| Helgeson, JP; Kemp, JD; Ursic, D | 1 |
| Charest, H; Matlashewski, G; Zhang, WW | 1 |
| Broxmeyer, HE; Cooper, S; Heimfeld, S; Kang, LY; Ruggieri, L; Srivastava, A; Zhou, SZ | 1 |
| Antonelli, A; Elli, R; Marcucci, L; Petrinelli, P | 1 |
| Hamann, L; Nickel, R; Tannich, E | 1 |
| Nakamura, I; Zheng, W | 1 |
| Kai, N; Kohmura, N; Mishina, M; Sanbo, M; Watanabe, S; Yagi, T; Yasuda, M | 1 |
| Ehrlich, M; Ji, W; Qu, GZ; Warshamana, GS; Zhang, XY | 1 |
| Daines, S; Evans, M; Vaulont, S | 1 |
| Camp, AM; Helm, BA; Pullar, CE; Wilson, AP | 1 |
| Barber, GN; Galabru, J; Hovanessian, AG; Katze, MG; Meurs, EF | 1 |
| Abramian, DS; Glebov, OK; Romanov, SR; Smagina, LV | 1 |
| Harrison, RW; Lippman, SS; VerHoeven, R | 1 |
| Matsuzaki, T; Murata, S; Noda, M; Takai, S; Yaoita, H | 1 |
| Clare, JJ; McCombie, WR; Romanos, MA; Scorer, CA; Sreekrishna, K | 1 |
| Kohn, DB; Nolta, JA; Smogorzewska, EM | 1 |
| Dingermann, T; Tillner, J; Winckler, T | 1 |
| Giga-Hama, Y; Kumagai, H; Okada, H; Okayama, H; Tohda, H | 1 |
| De Crombrugghe, B; Garofalo, S; Lefebvre, V; Metsäranta, M; Vuorio, E; Zhou, G | 1 |
| Kraus, G; Leavitt, MC; Looney, D; Poeschla, E; Wong-Staal, F; Yamada, O; Yu, M | 1 |
| Fisher, PR; Wilczynska, Z | 1 |
| Lei, P; Mensa-Wilmot, K; Morris, JC; Shen, TY | 1 |
| Blau, HM; Mohler, WA | 1 |
| Katayama, K; Mori, Y; Murata, Y; Niidome, T; Sawada, K; Seto, T; Tanaka, I; Teramoto, T | 1 |
| Cornetta, K; Hoffman, R; Leemhuis, T; Leibowitz, D; Moen, RC; Moore, A; Tricot, G | 1 |
| Cao, J; Geballe, AP | 1 |
| Ellis, D; Gao, D; Li, ZF; Murphy, T; Sauerbier, A; Sauerbier, W | 1 |
| McMaster, WR; Webb, JR | 1 |
| Hanafusa, H; Inoue, H; Sabe, H; Tavoloni, N | 1 |
| Certoux, JM; Chiang, Y; Contassot, E; Deschaseaux, M; Keller, J; Lyons, R; Murphy, WJ; Reynolds, CW; Spence, S; Tiberghien, P | 1 |
| Coggan, JS; Kovacs, I; Thompson, SH | 1 |
| Hori, R; Okuda, M; Takano, M; Yasuhara, M | 1 |
| Dunckley, MG; Kumar, S; Lund, T; O'Dowd, C | 1 |
| Beverley, SM; Freedman, DJ | 1 |
| Bevilacqua, MA; Cimino, F; Costanzo, F; Faniello, MC; Russo, T | 1 |
| Bradshaw, G; Donnelly, RJ; Mariano, TM; Pestka, S; Soh, J | 1 |
| Byers, TJ; Hugo, ER | 1 |
| Abe, N; Inagaki, T; Maeda, S; Matsuwari, S; Shimada, K; Takahashi, R | 1 |
| Boyle, TJ; Coles, RE; Kurtzberg, J; Lyerly, HK; Peters, WP; Stewart, A | 1 |
| Fujie, K; Inagaki, T; Maeda, S; Matsuwari, S; Shimada, K; Takahashi, R | 1 |
| Chittenden, T; Kaelin, W; Livingston, DM; Qin, X | 1 |
| Beverley, SM; Dasgupta, S; Ryan, KA | 1 |
| Ascoli, M; Stocco, DM | 1 |
| Hubert, WG; Lambert, PF | 1 |
| Blasi, F; Møller, LB; Pedersen, N; Pöllänen, J; Rønne, E | 1 |
| Bai, L; Carnie, S; Dahm, MW; Norris, JS; Schwartz, DA | 1 |
| Chan, MT; Chang, HH; Ho, SL; Tong, WF; Yu, SM | 1 |
| Japón, MA; Low, MJ; Rubinstein, M | 1 |
| Frisque, RJ; Trowbridge, PW | 1 |
| McDaniel, LD; Schultz, RA | 1 |
| Bierer, BE; Germain, RN; Hahn, WC; Menzin, E; Saito, T | 1 |
| Hu, DN; McCormick, SA; Pelton-Henrion, K; Ritch, R | 1 |
| Robbins, J | 1 |
| Bodmer, WF; Markie, D; Ragoussis, J; Rowan, A; Sansom, D; Senger, G; Sheer, D; Trowsdale, J | 1 |
| Murphy, NB; Muthiani, AM; Peregrine, AS | 1 |
| Liber, HL; Xia, F | 1 |
| DuBow, MS; Saint-Dic, D | 1 |
| Gottesman, MM; Li, F; Linton, GF; Malech, HL; Sekhsaria, S; Sokolic, RA; Sugimoto, Y; Whiting-Theobald, N | 1 |
| Bedwell, DM; Frizzell, RA; Howard, M | 1 |
| Doroshow, JH; Kane, SE; Matsumoto, L; Metz, MZ; Winters, KA | 1 |
| Hume, DA; Sweet, MJ | 1 |
| Cross, GA; Davies, KP; Muñoz-Jordán, JL | 1 |
| Mensa-Wilmot, K; Morris, JC; Ping-Sheng, L; Shen, TY; Zhai, HX | 1 |
| Galand, P; Horman, S; Mairesse, N; Mosselmans, R | 1 |
| Baiocchi, M; Bona, R; Chelucci, C; D'Aloja, P; Federico, M; Mavilio, F; Nappi, F; Pugliese, K; Verani, P | 1 |
| Field, LJ; Klug, MG; Koh, GY; Soonpaa, MH | 1 |
| Fujioka, M; Kitazawa, S; Maeda, S; Shimada, K | 1 |
| Campbell, C; Hirsch, B; Johnson, BL; Krueger, L; Thyagarajan, B | 1 |
| Bol'sheva, NL; Egorov, EE; Fedorova, LI; Kost-alimova, MV; Lopukhina, AV; Lupatov, AIu; Zelenin, AV | 1 |
| Brookes, AJ; Lawson, D; Maule, J; Porteous, DJ; Slorach, EM; Watson, JE | 1 |
| Drews, RE; Kolker, MT; Moran, CP; Sachar, DS; Schnipper, LE | 1 |
| Light, Y; Marais, R; Martin, J; Spooner, RA; Springer, CJ | 1 |
| Ansell, R; Colman, A; Kind, AJ; McWhir, J; Schnieke, AE; Scott, AR; Wallace, H | 1 |
| Berlioz, C; Darlix, JL; Torrent, C | 1 |
| Blanpied, C; Chavanel, G; Goupy, C; Gustafsson, K; Hirsch, F; Hirsch, R; Panczak, A; Poncet, P | 1 |
| Horikawa, T; Morelli, JG; Norris, DA; Zekman, T | 1 |
| Dubart, A; Duménil, D; Goncalves, F; Lacout, C; Vainchenker, W | 1 |
| Brozmanová, J; Chovanec, M; Farkasová, E; Henriques, JA; Näslund, M; Slaninová, M; Vlcková, V | 1 |
| Rittling, SR | 1 |
| Gooding, C; Roberts, GC; Smith, CW | 1 |
| Fedortseva, RF; Iartseva, NV; Ignatova, TN; Pugovkina, NA | 1 |
| Lu, Y; Miyakoshi, J; Noda, A; Takebe, H; Yagi, T; Yamagishi, N | 1 |
| Beattie, GM; Hayek, A; Levine, F; Wang, S | 1 |
| Wang, AL; Wang, CC; Yu, DC | 1 |
| Adler, S; Adler, SG; Artishevsky, A; Cha, DR; Feld, S; Glassock, RJ; Nast, CC | 1 |
| Boorstein, RJ; Chaung, W | 1 |
| Gutker, D; Renniers, AC; van Ooyen, AJ; Verdoes, JC; Wery, J | 1 |
| Byun, J; Kim, JM; Kim, S; Kim, SH; Robbins, PD; Yim, J | 1 |
| Bethune, C; Bui, T; Ho, RJ; Kay, MA; Liu, ML | 1 |
| Tavoloni, N | 1 |
| Brodbeck, U; Bütikofer, P; Küng, M; Stadelmann, B | 1 |
| Gubin, AN; Miller, JL; Njoroge, JM; Reddy, B | 1 |
| Cazzalini, O; Foury, F | 1 |
| Alberti-Segui, C; Brachat, A; Philippsen, P; Rebischung, C; Wach, A | 1 |
| Ishiguro, K; Sartorelli, AC | 1 |
| Ito, M; Kawano, Y; Kim, HJ; Kuroda, Y; Ogose, T; Okamoto, Y; Shimada, T; Shimosaka, A; Takaue, Y; Tohda, Y; Watanabe, T; Yamaue, T | 1 |
| Burt, DW; Clark, AJ; Mountford, PS; Ramage, AD; Smith, AG | 1 |
| Blake, J; Hughes, SM; Salinas, PC | 1 |
| Hernández, R; López, I; Martínez-Calvillo, S | 1 |
| Hamilton, R; Keir, SD; Miller, J; Samulski, RJ; Tornatore, C; Xiao, X; Yu, G | 1 |
| Campbell, KH; Colman, A; Kind, AJ; Mycock, K; Ritchie, M; Ritchie, WA; Schnieke, AE; Scott, AR; Wilmut, I | 1 |
| Asselin, C; Bastin, M; Sullivan, M | 1 |
| Fliedner, TM; Meier, T; Müller, R; Thomas, CA; Weber, L; Ziegler, BL | 1 |
| Clapp, DW; Hock, JM; Long, H; Onyia, JE | 1 |
| Armour, CA; Dawson, CW; Eliopoulos, AG; Wakelam, MJ; Young, LS | 1 |
| Campain, JA; Hami, LS; Harrison, GS; Shpall, EJ; Terrell, KL; Tomczak, JA | 1 |
| Bärtsch, S; Sengstag, C; Würgler, FE | 1 |
| Alon, RN; Bracha, R; Mirelman, D | 1 |
| Chinswangwatanakul, W; Gordon, MY; Lewis, JL; Manning, M; Roberts, IA | 1 |
| Baganz, F; Gardner, DC; Hayes, A; Marren, D; Oliver, SG | 1 |
| Barth, C; Fisher, PR; Fraser, DJ | 1 |
| Frazer, A; Graveley, RM; Hatton, PV; Hollander, AP; Houghton, A; Oyajobi, BO; Russell, RG; Stringer, BM; Xu, C | 1 |
| Hernando, Y; Pearson, BM; Schweizer, M | 1 |
| Chou, CF; Sun, CH; Tai, JH | 1 |
| Buckingham, M; Chang, TH; Primig, M | 1 |
| Balakrishnan, R; Griffiths, JK; Tzipori, S; Widmer, G | 1 |
| Anderson, WF; Eglitis, M; Flake, AW; Moen, RC; Porada, CD; Tran, N; Troutman, L; Zanjani, ED; Zhao, Y | 1 |
| Bähler, J; Longtine, MS; McKenzie, A; Philippsen, P; Pringle, JR; Shah, NG; Steever, AB; Wach, A; Wu, JQ | 1 |
| Cohen-Solal, K; Cramer, E; Duménil, D; Féger, F; Goncalves, F; Guichard, J; Lacout, C; Vainchenker, W | 1 |
| Chan, V; Drews, R; Kolker, M; Moran, C; Sachar, D; Schnipper, L | 1 |
| Bahner, I; King, SR; Kohn, DB; Nabel, GJ; Ranga, U; Verma, S; Woffendin, C; Xu, L; Yang, ZY | 1 |
| DeBerardinis, RJ; Kazazian, HH; Moran, JV | 1 |
| Bouhassira, EE; Fiering, S; Garrick, D; Goeke, S; Magis, W; Martin, DI; Scalzo, D; Walters, MC; Whitelaw, E | 1 |
| Calabrò, V; Di Cristofano, A; La Mantia, G; Parisi, T | 1 |
| Hawkins, RL; Nakamura, M | 1 |
| Borth, N; Katinger, H; Kunert, R; Steinfellner, W; Strutzenberger, K | 1 |
| Bartenschlager, R; Herian, U; Koch, J; Körner, F; Lohmann, V; Theilmann, L | 1 |
| Beverley, SM; Gluzman, IY; Goldberg, DE; Goyard, S; Mamoun, CB | 1 |
| Bender, J; Kämpfer, M; Klein, A | 1 |
| Bradwin, G; Milstone, DS; Mortensen, RM | 1 |
| Liebman, SW; Mankin, AS | 1 |
| Donelson, JE; Downey, N | 1 |
| Daigo, Y; Kawasoe, T; Nakamura, Y; Nishiwaki, T | 1 |
| Hua, J; Lodge, JK; Meyer, JD | 1 |
| Laurent, JP; Swindle, J | 1 |
| Di Florio, S; Di Ianni, M; Falzetti, F; Mannoni, P; Martelli, MF; Tabilio, A; Venditti, G | 1 |
| Carignani, G; Mazzotta, G; Pavanello, A; Sartori, G; Stocchetto, S | 1 |
| Cossons, NH; Nielsen, TO; Price, GB; Zannis-Hadjopoulos, M | 1 |
| Al-Rubeai, M; Piacentini, M; Piredda, L; Singh, RP; Tey, BT | 1 |
| Holloman, WK; Kojic, M | 1 |
| Bolognia, JL; Chakraborty, AK; Kolesnikova, N; Pawelek, JM; Platt, JT; Rachkovsky, M; Sodi, S | 1 |
| Di Florio, S; Di Ianni, M; Falzetti, F; Liberatore, C; Lucheroni, F; Mannoni, P; Martelli, MF; Spinozzi, F; Stella, CC; Tabilio, A; Terenzi, A; Venditti, G | 1 |
| Dong, W; Guo, Z; Li, B; Li, J; Liang, CC; Liu, D | 1 |
| Bedwell, DM; Keeling, K; Manuvakhova, M | 1 |
| Grogan, L; Hausner, P; Kirsch, IR; Venzon, DJ | 1 |
| Derfoul, A; Hall, DJ; Izzo, MW; Min, I; Rallapalli, R; Stubbs, MC | 1 |
| Cox, GS | 1 |
| de Freitas, LH; Ramirez, MI; Schenkman, S; Uemura, H; Yamauchi, LM | 1 |
| Aliño, SF; Crespo, A; Escrig, E; Guillem, VM; Revert, F | 1 |
| Chang, CR; Cheng, TH; Gartenberg, MR; Joy, P; Yablok, S | 1 |
| Agarwal, A; Nick, HS; Shiraishi, F; Visner, GA | 1 |
| Ariga, T; Ezaki, I; Kawamura, N; Misaki, Y; Sakiyama, Y; Yamamoto, K | 1 |
| Birnboim, HC; Haqqani, AS; Sandhu, JK | 1 |
| Alagón, A; Benatti, U; Boffa, LC; Olvera, A; Ramos, MA; Sánchez, R; Sanchez-Lopez, R; Saralegui, A; Scarfì, S; Stock, RP | 1 |
| Ji, ZW; Komori, T; Oku, N | 1 |
| Bruner, M; Czymmek, K; Kmiec, EB; Rice, MC | 1 |
| Richards, JD; Shaw, JM; Stillman, DJ; Voth, WP | 1 |
| Casanova, P; Dalcq, MD; Darlix, JL; Feigenbaum-Lacombe, V; Franceschini, IA; Lopez-Lastra, M | 1 |
| Donohue, CA; Doroshow, JH; Kane, SE; Lin, SW; Lingeman, RG; Matsumoto, L; Metz, MZ | 1 |
| Breyer, B; Cheng, H; Feng, T; Haydon, R; He, TC; Ishikawa, A; Jiang, W; Zhou, L | 1 |
| Carroll, RC; Costanza, MJ; Donnell, RL; Freeman, MB; Goldman, MH; Grandas, OH; Reddick, TT; Stevens, SL | 1 |
| Holbrook, MA; Menninger, JR | 1 |
| Apperley, JF; Bodinier, M; Duperrier, A; Ferrand, C; Garin, M; Hervé, P; Lang, F; Melo, JV; Petracca, B; Robinet, E; Sauce, D; Tiberghien, P; Tonnelier, N | 1 |
| Hall, EJ; Hopkins, KM; Lieberman, HB; Randers-Pehrson, G; Sawant, SG; Zheng, W | 1 |
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| Harris, S; Hearn, MT; Papakonstantinou, T | 1 |
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| Guo, R; Jia, Y; Ke, X; Shi, B; Xie, J; Yu, H | 1 |
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4 review(s) available for antibiotic g 418 and gentamicin
| Article | Year |
|---|---|
Cellular aspects of circadian rhythms.
Topics: Acetabularia; Anti-Bacterial Agents; Cell Membrane; Chloroplasts; Circadian Rhythm; Drug Resistance; Gentamicins; Membrane Potentials; Models, Biological; Photosynthesis; Protein Biosynthesis | 1986 |
Antibiotic from Micromonospora.
Topics: Aminoglycosides; Anti-Bacterial Agents; Chemical Phenomena; Chemistry; Dactinomycin; Erythromycin; Gentamicins; Glycosides; Lactams, Macrocyclic; Leucomycins; Neomycin; Pyrrolidines; Rifamycins; Sisomicin; Streptomycetaceae | 1980 |
Structural and functional contributions to the G1 blocking action of the retinoblastoma protein. (the 1992 Gordon Hamilton Fairley Memorial Lecture).
Topics: Cell Division; G1 Phase; Gentamicins; Humans; Osteosarcoma; Recombinant Fusion Proteins; Retinoblastoma Protein; Transfection; Tumor Cells, Cultured | 1993 |
Gene targeting. The precise manipulation of the mammalian genome.
Topics: Animals; Arteriosclerosis; Cardiovascular Diseases; Cardiovascular Physiological Phenomena; Cell Line; Chimera; Disease Susceptibility; Drug Resistance; Forecasting; Genome; Gentamicins; Humans; Hypoxanthine Phosphoribosyltransferase; Male; Mice; Mutagenesis, Insertional; Recombination, Genetic; Stem Cells; Transfection | 1993 |
484 other study(ies) available for antibiotic g 418 and gentamicin
| Article | Year |
|---|---|
[Experimental study on the renal compatibility of the aminoglycosides gentamicin B and O-2-amino-2-deoxy-6-C-methyl-alpha-D-glucopyranosyl-(1 greater than 4)-garamine (G-418)].
Topics: Animals; Enzymes; Female; Gentamicins; Kidney Diseases; Proteinuria; Rats | 1979 |
Comparison of a new aminoglycoside (G-418) and gentamicin for the treatment of swine dysentery in swine naturally infected with Salmonella choleraesuis.
Topics: Administration, Oral; Aminoglycosides; Animals; Anti-Bacterial Agents; Dysentery; Gentamicins; Salmonella Infections, Animal; Swine; Swine Diseases; Water | 1977 |
Antibiotic G418, a new micrommomospora-produced aminglycoside with activity apainst protozoa and helminths: antiparasitic activity.
Topics: Aminoglycosides; Animals; Anti-Bacterial Agents; Dysentery, Amebic; Gentamicins; Male; Mice; Mice, Inbred Strains | 1975 |
Selectable markers for rice transformation.
Topics: Agrobacterium tumefaciens; Aminobutyrates; Bleomycin; Cinnamates; DNA, Recombinant; Drug Resistance; Electric Stimulation; Gene Expression; Genetic Markers; Gentamicins; Herbicides; Hygromycin B; Kanamycin; Kanamycin Kinase; Methotrexate; Oryza; Phosphotransferases; Protoplasts; Recombinant Proteins; Transfection; Transformation, Genetic | 1992 |
Regulation of G418 selection efficiency by cell-cell interaction in transfection.
Topics: Animals; Calcium Phosphates; Cell Communication; Cell Count; Cell Division; Cells, Cultured; Cricetinae; Cricetulus; Dimethyl Sulfoxide; DNA, Recombinant; Drug Resistance; Gentamicins; Intercellular Junctions; Mice; Plasmids; Rats; Tetradecanoylphorbol Acetate; Time Factors; Transfection | 1992 |
Polyomavirus large and small T antigens cooperate in induction of the S phase in serum-starved 3T3 mouse fibroblasts.
Topics: 3T3 Cells; Animals; Antigens, Polyomavirus Transforming; Blotting, Northern; Cinnamates; Culture Media, Serum-Free; Dexamethasone; DNA Replication; DNA, Viral; Flow Cytometry; Gene Expression Regulation, Viral; Gentamicins; Hygromycin B; Mice; Plasmids; Polyomavirus; S Phase; Transfection | 1992 |
The nature of extra-chromosomal maintenance of transforming plasmids in the filamentous basidiomycete Phanerochaete chrysosporium.
Topics: Basidiomycota; Cytosine; DNA Restriction Enzymes; Drug Resistance, Microbial; Genetic Vectors; Gentamicins; Methylation; Plasmids; Transformation, Genetic | 1992 |
An approach for the analysis of relapse and marrow reconstitution after autologous marrow transplantation using retrovirus-mediated gene transfer.
Topics: Bone Marrow; Bone Marrow Transplantation; Cell Division; Cells, Cultured; Colony-Forming Units Assay; Genetic Markers; Gentamicins; Hematopoietic Stem Cells; Humans; Kanamycin Kinase; Leukemia, Myeloid, Acute; Phosphotransferases; Probability; Recurrence; Retroviridae; Transfection; Transplantation, Autologous | 1992 |
A highly conserved tyrosine residue at codon 845 within the kinase domain is not required for the transforming activity of human epidermal growth factor receptor.
Topics: 3T3 Cells; Animals; Base Sequence; Codon; DNA Replication; Drug Resistance; Epidermal Growth Factor; ErbB Receptors; Gentamicins; Humans; Mice; Molecular Sequence Data; Mutagenesis, Site-Directed; Oligodeoxyribonucleotides; Phenylalanine; Protein-Tyrosine Kinases; Transfection; Tyrosine | 1992 |
Growth of HeLa S3 cells cotransfected with plasmids containing a c-fos gene under the control of the SV40 promoter complex, pRSVcat, and G418 resistance.
Topics: Acetyltransferases; Culture Media, Serum-Free; Gene Expression; Genes, fos; Genes, Synthetic; Genetic Vectors; Gentamicins; HeLa Cells; Humans; Interphase; Kanamycin Resistance; Plasmids; Promoter Regions, Genetic; Proto-Oncogene Proteins c-fos; Recombinant Fusion Proteins; Simian virus 40; Transfection | 1992 |
Ligand-independent reduction of cAMP receptors in Dictyostelium discoideum cells over-expressing a mutated ras gene.
Topics: Animals; Blotting, Northern; Blotting, Western; Cyclic AMP; Dictyostelium; Drug Resistance, Microbial; Gene Expression Regulation, Fungal; Genes, ras; Gentamicins; Mutagenesis; Phenotype; Receptors, Cyclic AMP; RNA, Messenger; Transformation, Genetic | 1992 |
Secretion of Aspergillus oryzae alkaline protease in an osmophilic yeast, Zygosaccharomyces rouxii.
Topics: Amino Acid Sequence; Aspergillus oryzae; Base Sequence; DNA; DNA Restriction Enzymes; Drug Resistance, Microbial; Enzyme Precursors; Gene Expression; Gentamicins; Glyceraldehyde-3-Phosphate Dehydrogenases; Molecular Sequence Data; Plasmids; Promoter Regions, Genetic; Saccharomycetales; Serine Endopeptidases | 1990 |
Terminal regions of the NS-1 protein of the parvovirus minute virus of mice are involved in cytotoxicity and promoter trans inhibition.
Topics: Animals; Capsid; Cell Survival; DNA Replication; DNA, Viral; Drug Resistance, Microbial; Electrophoresis, Agar Gel; Gentamicins; Mice; Minute Virus of Mice; Mutation; Plasmids; Promoter Regions, Genetic; Transcription Factors; Transcriptional Activation; Transformation, Genetic; Viral Core Proteins; Viral Nonstructural Proteins | 1992 |
Retrovirus-mediated gene transfer as an approach to analyze neuroblastoma relapse after autologous bone marrow transplantation.
Topics: Base Sequence; Bone Marrow Transplantation; DNA; Drug Resistance; Genetic Markers; Gentamicins; Humans; Molecular Sequence Data; Neoplasm Recurrence, Local; Neuroblastoma; Phenotype; Polymerase Chain Reaction; Retroviridae; Transduction, Genetic; Transfection; Transplantation, Autologous | 1992 |
Use of cell-free retroviral vector preparations for transduction of cells from the marrow of chronic phase and blast crisis chronic myelogenous leukemia patients and from normal individuals.
Topics: Base Sequence; Blast Crisis; Bone Marrow; Bone Marrow Cells; Cell Division; Cell-Free System; DNA; Drug Resistance; Genetic Vectors; Gentamicins; Hematopoietic Stem Cells; Humans; Leukemia, Myeloid, Chronic-Phase; Molecular Sequence Data; Retroviridae; Transduction, Genetic | 1992 |
A chimaeric antibiotic resistance gene as a selectable marker for plant cell transformation. 1983.
Topics: Agrobacterium tumefaciens; Drug Resistance; Genetic Vectors; Gentamicins; History, 20th Century; Plants; Transformation, Genetic | 1992 |
Effects of exogenous wild-type p53 on a human lung carcinoma cell line with endogenous wild-type p53.
Topics: Cell Division; Dexamethasone; Gene Expression; Genes, p53; Gentamicins; Humans; Plasmids; S Phase; Transfection; Tumor Cells, Cultured; Tumor Suppressor Protein p53 | 1992 |
Enhanced sensitivity to G418 of human KB cells adapted to certain media and sera.
Topics: Blood; Cell Separation; Culture Media; Dose-Response Relationship, Drug; Drug Resistance; Gentamicins; Humans; KB Cells; Transfection | 1992 |
Spontaneous and mutagen-mediated amplification of a neo gene integrated at different genomic sites in rat 2 fibroblasts.
Topics: Animals; Blotting, Southern; Drug Resistance; Fibroblasts; Gene Amplification; Gene Expression Regulation, Neoplastic; Gentamicins; Methyl Methanesulfonate; Mitomycin; Rats; Transfection | 1992 |
Partial phenotypic suppression of a peroxisome-deficient animal cell mutant treated with aminoglycoside G418.
Topics: Acyltransferases; Animals; Autoradiography; CHO Cells; Chromatography, Thin Layer; Cricetinae; Cricetulus; Gentamicins; Microbodies; Microscopy, Fluorescence; Oxidation-Reduction; Phenotype; Phospholipids; Plasmalogens | 1992 |
High-fidelity gene targeting in embryonic stem cells by using sequence replacement vectors.
Topics: Animals; Drug Resistance; Gene Rearrangement; Genetic Vectors; Gentamicins; Mice; Mutagenesis, Site-Directed; Recombination, Genetic; Selection, Genetic; Stem Cells; Thioguanine; Transformation, Genetic | 1992 |
L-histidinol provides effective selection of retrovirus-vector-transduced keratinocytes without impairing their proliferative potential.
Topics: Adenosine Deaminase; Alcohol Oxidoreductases; Animals; Cell Division; Cells, Cultured; Dogs; Drug Resistance, Microbial; Gene Expression; Genetic Vectors; Gentamicins; Histidinol; Kanamycin Kinase; Keratinocytes; Phosphotransferases; Recombinant Fusion Proteins; Selection, Genetic | 1991 |
A pair of selectable herpesvirus vectors for simultaneous gene expression in human lymphoid cells.
Topics: Base Sequence; Cells, Cultured; Cinnamates; Cloning, Molecular; DNA, Recombinant; DNA, Viral; Drug Resistance, Microbial; Electrophoresis, Agar Gel; Gene Expression Regulation; Genetic Vectors; Gentamicins; Herpesvirus 2, Saimiriine; Humans; Hygromycin B; Molecular Sequence Data; T-Lymphocytes | 1991 |
Persistence of selectable herpesvirus saimiri in various human haematopoietic and epithelial cell lines.
Topics: Blotting, Northern; Cell Line; Connective Tissue; Drug Resistance; Epithelial Cells; Epithelium; Genes, Viral; Genetic Vectors; Gentamicins; Hematopoietic Stem Cells; Herpesvirus 2, Saimiriine; Humans; Hygromycin B; Phenotype; Plasmids; Transfection; Virus Replication | 1991 |
Promoter activity associated with the left inverted terminal repeat of the killer plasmid k1 from yeast.
Topics: Base Sequence; Culture Media; DNA Transposable Elements; DNA-Directed DNA Polymerase; Drug Resistance, Microbial; Escherichia coli; Gentamicins; Killer Factors, Yeast; Kluyveromyces; Lac Operon; Molecular Sequence Data; Mycotoxins; Plasmids; Promoter Regions, Genetic; Repetitive Sequences, Nucleic Acid; Transcription, Genetic; Transformation, Genetic | 1991 |
Efficient selection for high-expression transfectants with a novel eukaryotic vector.
Topics: Actins; Animals; beta-Galactosidase; Blotting, Northern; Blotting, Southern; Bovine papillomavirus 1; Cattle; CHO Cells; Cricetinae; Genetic Vectors; Gentamicins; Humans; Interleukin-2; Kanamycin Kinase; L Cells; Mice; Phosphotransferases; Promoter Regions, Genetic; Recombinant Proteins; Selection, Genetic; Transfection | 1991 |
An in vitro 125IUdR-release assay for measuring the kinetics of cell death.
Topics: Animals; Cell Survival; Cold Temperature; Gentamicins; Hot Temperature; Idoxuridine; In Vitro Techniques; Iodine Radioisotopes; Kinetics | 1991 |
Immortalization of primary rat embryo cells by human papillomavirus type 11 DNA is enhanced upon cotransfer of ras.
Topics: Animals; Anti-Bacterial Agents; Blotting, Northern; Blotting, Southern; Cell Division; Cell Transformation, Viral; Clone Cells; DNA, Viral; Drug Resistance; Embryo, Mammalian; Female; Genes, ras; Gentamicins; Humans; Kinetics; Neoplasms, Experimental; Papillomaviridae; Plasmids; Rats; Rats, Inbred F344; RNA; Transfection | 1990 |
A novel BK virus-based episomal vector for expression of foreign genes in mammalian cells.
Topics: BK Virus; Blotting, Northern; Blotting, Southern; Chloramphenicol O-Acetyltransferase; Chromatography, Affinity; Cloning, Molecular; DNA; Drug Resistance; Gene Amplification; Genetic Vectors; Gentamicins; HeLa Cells; Humans; Plasmids; RNA; Simian virus 40; Transfection; Transformation, Bacterial; Transformation, Genetic | 1991 |
Embryonic mouse spinal cord motor neuron hybrid cells.
Topics: Aminopterin; Animals; Cell Fusion; Choline O-Acetyltransferase; Chromosomes; Clone Cells; Drug Resistance; Gentamicins; Glucose-6-Phosphate Isomerase; Hybrid Cells; Hypoxanthine Phosphoribosyltransferase; Isoenzymes; Mice; Mice, Inbred C57BL; Motor Neurons; Neuroblastoma; Selection, Genetic; Spinal Cord; Tumor Cells, Cultured | 1991 |
Replicative transformation of the filamentous fungus Ashbya gossypii with plasmids containing Saccharomyces cerevisiae ARS elements.
Topics: Ascomycota; DNA, Recombinant; Drug Resistance, Microbial; Electrophoresis, Gel, Pulsed-Field; Genetic Markers; Genetic Vectors; Gentamicins; Kanamycin Resistance; Karyotyping; Plasmids; Protoplasts; Saccharomyces; Transformation, Genetic | 1991 |
Isolation of mouse cells with heavy metal-dependent resistance to G418.
Topics: Animals; Blotting, Northern; Blotting, Southern; Clone Cells; Drug Resistance, Microbial; Gene Expression Regulation; Gentamicins; Metallothionein; Mice; Transformation, Genetic | 1991 |
Hygromycin- and paromomycin-resistant mutants of Aspergillus nidulans alter translational fidelity.
Topics: Aspergillus nidulans; Drug Resistance, Microbial; Genes, Fungal; Gentamicins; Hygromycin B; Mutation; Paromomycin; Phenotype; Protein Biosynthesis | 1991 |
Selection of gene-marked tumor infiltrating lymphocytes from post-treatment biopsies: a case study.
Topics: Biopsy; Cells, Cultured; Female; Gene Rearrangement, T-Lymphocyte; Genetic Markers; Genetic Vectors; Gentamicins; Graft Survival; Humans; Immunotherapy, Adoptive; Interleukin-2; Kanamycin Kinase; Lymphocytes, Tumor-Infiltrating; Melanoma; Phosphotransferases; Recombinant Proteins; Remission Induction; Selection, Genetic; Skin Neoplasms | 1990 |
In vivo expression and survival of gene-modified T lymphocytes in rhesus monkeys.
Topics: Adenosine Deaminase; Animals; Base Sequence; DNA, Recombinant; Female; Gene Expression; Genetic Engineering; Genetic Vectors; Gentamicins; Graft Survival; Kanamycin Kinase; Macaca mulatta; Molecular Sequence Data; Phosphotransferases; Polymerase Chain Reaction; Recombinant Fusion Proteins; Retroviridae; T-Lymphocytes | 1990 |
Determination of retroviral mutation rates using spleen necrosis virus-based vectors and helper cells.
Topics: Animals; Base Composition; Base Sequence; Cinnamates; Dogs; Drug Resistance; Gene Expression; Genes, Viral; Genetic Vectors; Gentamicins; Hygromycin B; Molecular Sequence Data; Mutation; Osteosarcoma; Polymerase Chain Reaction; Proviruses; Restriction Mapping; Retroviridae; Transfection; Tumor Cells, Cultured; Virus Replication | 1991 |
Retrovirus-mediated gene transfer into CD4+ and CD8+ human T cell subsets derived from tumor-infiltrating lymphocytes and peripheral blood mononuclear cells.
Topics: Blotting, Southern; Cell Division; Cell Survival; Cells, Cultured; DNA; Genes, Bacterial; Genetic Markers; Gentamicins; Humans; Interleukin-2; Leukocytes, Mononuclear; Lymphocytes, Tumor-Infiltrating; Phenotype; Retroviridae; Transduction, Genetic | 1991 |
Lymphocytes as cellular vehicles for gene therapy in mouse and man.
Topics: Animals; Cells, Cultured; Genetic Therapy; Genetic Vectors; Gentamicins; Humans; In Vitro Techniques; Lymphocyte Activation; Mice; Retroviridae; T-Lymphocytes; Transduction, Genetic; Transfection | 1991 |
BALB/C mouse 3T3 fibroblasts expressing human estrogen receptor: effect of estradiol on cell growth.
Topics: Animals; Cell Division; Chloramphenicol O-Acetyltransferase; DNA Replication; Estradiol; Fibroblasts; Gentamicins; Humans; Kinetics; Mice; Mice, Inbred BALB C; Proto-Oncogenes; Receptors, Estrogen; RNA, Messenger; Transfection | 1991 |
Transfection of wild-type and 'Fanconi anemia-like' mouse lymphoma mutant cells by electroporation.
Topics: Animals; Blotting, Southern; Drug Resistance; Electric Stimulation; Fanconi Anemia; Fluorescent Dyes; Genetic Techniques; Gentamicins; Isoquinolines; Lymphoma; Mice; Mutation; Transfection; Tumor Cells, Cultured | 1991 |
MoMuLV-derived self-inactivating retroviral vectors possessing multiple cloning sites and expressing the resistance to either G418 or hygromycin B.
Topics: Base Sequence; Chromosome Mapping; Cloning, Molecular; DNA, Viral; Drug Resistance, Microbial; Genetic Vectors; Gentamicins; Hygromycin B; Molecular Sequence Data; Plasmids; Recombination, Genetic; Restriction Mapping; Retroviridae | 1990 |
Intrachromosomal homologous recombination in human cells which differ in nucleotide excision-repair capacity.
Topics: Blotting, Southern; Cell Survival; Culture Media; DNA; DNA Repair; Drug Resistance; Genes, Viral; Gentamicins; Humans; Plasmids; Recombination, Genetic; Simplexvirus; Thymidine Kinase; Transfection; Tumor Cells, Cultured; Ultraviolet Rays | 1990 |
Rapid titration of retroviral vectors encoding intracellular antigens by flow cytometry.
Topics: Animals; Anti-Bacterial Agents; Antibodies, Monoclonal; Antigens, Polyomavirus Transforming; Cell Cycle; Cell Transformation, Viral; Cells, Cultured; Drug Resistance; Flow Cytometry; Fluorescent Antibody Technique; Genetic Vectors; Gentamicins; Mice; Mice, Inbred Strains; Retroviridae; Simian virus 40 | 1990 |
Construction and hormone regulation of a novel retroviral vector.
Topics: Animals; Chloramphenicol O-Acetyltransferase; Cloning, Molecular; Dexamethasone; Drug Resistance, Microbial; Gene Expression Regulation, Viral; Genetic Engineering; Genetic Vectors; Gentamicins; Mammary Tumor Virus, Mouse; Mice; Plasmids; Promoter Regions, Genetic; Repetitive Sequences, Nucleic Acid; Retroviridae | 1990 |
Recombinant gene expression in human umbilical vein endothelial cells transduced by retroviral vectors.
Topics: Anti-Bacterial Agents; Blotting, Southern; DNA, Recombinant; Endothelium, Vascular; Gene Expression; Genetic Vectors; Gentamicins; Humans; Kanamycin Kinase; Kanamycin Resistance; Phosphotransferases; Recombinant Proteins; Retroviridae; Transduction, Genetic | 1990 |
Expression and characterization of rat surfactant protein A synthesized in Chinese hamster ovary cells.
Topics: Animals; Binding, Competitive; Blotting, Northern; Cell Line; Cricetinae; Drug Resistance; Gene Amplification; Gene Expression; Gentamicins; Kanamycin Kinase; Methotrexate; Phospholipids; Phosphotransferases; Plasmids; Proteolipids; Pulmonary Surfactant-Associated Protein A; Pulmonary Surfactant-Associated Proteins; Pulmonary Surfactants; Rats; Recombinant Proteins; Tetrahydrofolate Dehydrogenase; Transfection; Transformation, Genetic | 1990 |
G418-resistance as a dominant marker and reporter for gene expression in Saccharomyces cerevisiae.
Topics: Amino Acid Sequence; Base Sequence; DNA, Fungal; Gene Expression; Genes, Dominant; Genes, Fungal; Genetic Markers; Gentamicins; Kanamycin Kinase; Kanamycin Resistance; Molecular Sequence Data; Mutagenesis, Insertional; Phosphotransferases; Plasmids; Promoter Regions, Genetic; Protein Biosynthesis; RNA, Messenger; Saccharomyces cerevisiae; Transformation, Genetic | 1990 |
Homologous recombination and stable transfection in the parasitic protozoan Trypanosoma brucei.
Topics: Amanitins; Animals; Base Sequence; Chloramphenicol O-Acetyltransferase; DNA, Protozoan; Drug Resistance; Gene Expression; Genetic Vectors; Gentamicins; Kanamycin Kinase; Membrane Glycoproteins; Molecular Sequence Data; Nucleic Acid Hybridization; Phosphotransferases; Plasmids; Promoter Regions, Genetic; Protozoan Proteins; Recombination, Genetic; Restriction Mapping; Transcription, Genetic; Transfection; Trypanosoma brucei brucei; Tubulin; Variant Surface Glycoproteins, Trypanosoma | 1990 |
Construction of a neo fusion gene for expression in both prokaryotic and eukaryotic cells.
Topics: Animals; Base Sequence; Drug Resistance, Microbial; Escherichia coli; Gentamicins; Humans; Hypoxanthine Phosphoribosyltransferase; Mice; Molecular Sequence Data; Mutation; Neomycin; Peptide Chain Termination, Translational; Plasmids; Recombinant Fusion Proteins; Selection, Genetic | 1990 |
Transfection of cultured fish cells RBCF-1 with exogenous oncogene and their resistance to malignant transformation.
Topics: Animals; Anti-Bacterial Agents; Cell Division; Cell Line; Cell Transformation, Neoplastic; Cells, Cultured; Drug Resistance; Genes, ras; Gentamicins; Goldfish; Humans; Mice; Transfection; Urinary Bladder Neoplasms | 1990 |
Antisense RNA inhibition of HPRT synthesis.
Topics: Animals; Base Sequence; Cell Line; Chromosome Deletion; Drug Resistance; Exons; Gene Expression Regulation, Enzymologic; Gentamicins; Hypoxanthine Phosphoribosyltransferase; Introns; Molecular Sequence Data; Mutagenesis; Phenotype; Plasmids; RNA Processing, Post-Transcriptional; RNA Splicing; RNA, Antisense; RNA, Messenger; Thioguanine; Transfection | 1990 |
Unequal SCE is a rare event in homologous recombination between duplicated neo gene fragments in CHO cells.
Topics: Acetyltransferases; Animals; Cell Line; Cricetinae; DNA, Recombinant; Drug Resistance; Gentamicins; Methyl Methanesulfonate; Recombination, Genetic; Sister Chromatid Exchange | 1990 |
Long-term transplantation of canine keratinocytes made resistant to G418 through retrovirus-mediated gene transfer.
Topics: Animals; Anti-Bacterial Agents; Biopsy; Cell Line; Cells, Cultured; Dogs; Drug Resistance; Female; Genetic Vectors; Gentamicins; Keratinocytes; Male; Microscopy, Electron; Retroviridae; Skin | 1990 |
Gene transfer by retrovirus-derived shuttle vectors in the generation of murine bispecific monoclonal antibodies.
Topics: Animals; Anti-Bacterial Agents; Antibodies, Monoclonal; Cell Line; Cells, Cultured; Chromatography, High Pressure Liquid; Cytotoxicity, Immunologic; Drug Resistance; Genetic Vectors; Gentamicins; Humans; Hybridomas; Methotrexate; Mice; Retroviridae; T-Lymphocytes, Cytotoxic; Transfection | 1990 |
Infection of rat liver epithelial cells with v-Ha-ras: correlation between oncogene expression, gap junctional communication, and tumorigenicity.
Topics: Animals; Anti-Bacterial Agents; Cell Communication; Contact Inhibition; DNA; DNA Probes; Drug Resistance; Epithelium; Gene Expression; Genes, Viral; Gentamicins; Intercellular Junctions; Liver Neoplasms; Male; Methylation; Oncogenes; Rats; Rats, Inbred F344; RNA, Messenger; Tumor Cells, Cultured | 1990 |
Retroviral vector-mediated transfer of the bacterial neomycin resistance gene into fetal and adult sheep and human hematopoietic progenitors in vitro.
Topics: Animals; Bone and Bones; Bone Marrow; Colony-Stimulating Factors; Drug Resistance, Microbial; Gene Expression Regulation; Genetic Vectors; Gentamicins; Granulocyte-Macrophage Colony-Stimulating Factor; Growth Substances; Hematopoiesis; Hematopoietic Stem Cells; Humans; In Vitro Techniques; Interleukin-3; Liver; Neomycin; Retroviridae; Sheep; Transfection | 1990 |
Maintenance of expression of differentiated function of kidney cells following transformation by SV40 early region DNA.
Topics: Animals; Antigens, Viral, Tumor; Carrier Proteins; Cell Differentiation; Cell Line; DNA, Viral; Drug Resistance; Epithelial Cells; Epithelium; Genes, Viral; Gentamicins; Ion Channels; Kidney Tubules; Loop of Henle; Mucoproteins; Potassium; Rabbits; Simian virus 40; Sodium-Potassium-Chloride Symporters; Sodium-Potassium-Exchanging ATPase; Transfection; Uromodulin | 1986 |
Ability of structurally related polycyclic aromatic carcinogens to induce homologous recombination between duplicated chromosomal sequences in mouse L cells.
Topics: 4-Nitroquinoline-1-oxide; Acetoxyacetylaminofluorene; Animals; Anti-Bacterial Agents; Carcinogens; Cell Survival; Chromosomes; DNA Damage; Drug Resistance; Gentamicins; L Cells; Mice; Polycyclic Compounds; Pyrenes; Recombination, Genetic | 1989 |
Induced recombination between duplicated neo genes stably integrated in the genome of CHO cells.
Topics: Animals; Blotting, Southern; Cell Line; Cricetinae; Cricetulus; Drug Resistance; Genes, Bacterial; Gentamicins; Kanamycin Kinase; Mechlorethamine; Methyl Methanesulfonate; Mutation; Phosphotransferases; Plasmids; Recombination, Genetic | 1989 |
Inhibition of rat growth hormone promoter activity by site-specific DNA methylation.
Topics: Animals; Binding Sites; Cells, Cultured; Chloramphenicol O-Acetyltransferase; DNA; DNA-Cytosine Methylases; Drug Resistance; Gentamicins; Growth Hormone; Kanamycin Kinase; Methylation; Phosphotransferases; Plasmids; Promoter Regions, Genetic; Rats; Recombinant Fusion Proteins | 1989 |
Clonal populations of the mouse mammary cell line, COMMA-D, which retain capability of morphogenesis in vivo.
Topics: Adipose Tissue; Animals; Anti-Bacterial Agents; Caseins; Cell Line; Clone Cells; Cloning, Molecular; DNA Restriction Enzymes; Drug Resistance, Microbial; Female; Gentamicins; Histocytochemistry; Immunoenzyme Techniques; Lactation; Mammary Glands, Animal; Mice; Mice, Inbred BALB C; Morphogenesis; Plasmids; Pregnancy; Transfection | 1989 |
Molecular transformation of Fusarium solani with an antibiotic resistance marker having no fungal DNA homology.
Topics: Base Sequence; Cloning, Molecular; DNA Transposable Elements; DNA, Fungal; Drug Resistance, Microbial; Fusarium; Genetic Markers; Gentamicins; Molecular Sequence Data; Mosaic Viruses; Plasmids; Promoter Regions, Genetic; Restriction Mapping; RNA, Messenger; Sequence Homology, Nucleic Acid; Transcription, Genetic; Transformation, Genetic | 1989 |
Cauliflower mosaic virus promoters direct efficient expression of a bacterial G418 resistance gene in Schizosaccharomyces pombe.
Topics: Alcohol Dehydrogenase; DNA Transposable Elements; Drug Resistance, Microbial; Gene Expression; Gentamicins; Microbial Sensitivity Tests; Mosaic Viruses; Plasmids; Promoter Regions, Genetic; Saccharomycetales; Schizosaccharomyces; Transformation, Genetic | 1989 |
Graduated resistance to G418 leads to differential selection of cultured mammalian cells expressing the neo gene.
Topics: Animals; Anti-Bacterial Agents; Cell Division; Cell Line; Clone Cells; Cloning, Molecular; Drug Resistance; Gene Expression; Genetic Vectors; Gentamicins; Kanamycin Kinase; Kinetics; Neomycin; Phosphotransferases | 1989 |
Tumorigenicity of human mesothelial cell line transfected with EJ-ras oncogene.
Topics: Animals; Cell Line, Transformed; Cell Transformation, Neoplastic; Drug Resistance; Gentamicins; Humans; Mesothelioma; Mice; Mice, Nude; Neoplasm Transplantation; Oncogenes; Transfection | 1989 |
Isolation of a clone of F9 teratocarcinoma cells "naturally" resistant to G418.
Topics: Animals; Antibiotics, Antineoplastic; Bacterial Proteins; Clone Cells; Deoxyribonuclease BamHI; Deoxyribonucleases, Type II Site-Specific; DNA; DNA Restriction Enzymes; Drug Resistance; Fluorescent Antibody Technique; Gentamicins; Mice; Oncogenes; Phenotype; Plasmids; Teratoma; Transcription, Genetic; Transfection; Tumor Cells, Cultured | 1987 |
Improved mammalian vectors for high expression of G418 resistance.
Topics: Animals; Anti-Bacterial Agents; Base Sequence; Cell Line; Codon; Drug Resistance; Fibroblasts; Genetic Vectors; Gentamicins; Mice; Plasmids; Promoter Regions, Genetic; Protein Biosynthesis; Simian virus 40; Teratoma; Transfection; Tumor Cells, Cultured | 1987 |
Gene transfer into hematopoietic progenitor cells from normal and cyclic hematopoietic dogs using retroviral vectors.
Topics: Animals; Anti-Bacterial Agents; Bone Marrow; Bone Marrow Cells; Cell Cycle; Colony-Forming Units Assay; Dogs; Drug Resistance, Microbial; Female; Genetic Vectors; Gentamicins; Hematopoiesis; Hematopoietic Stem Cells; Kanamycin Kinase; Male; Moloney murine leukemia virus; Phosphotransferases; Transfection | 1988 |
An indicator gene to demonstrate intracellular transposition of defective retroviruses.
Topics: Defective Viruses; DNA Transposable Elements; DNA, Recombinant; Drug Resistance; Genes, Synthetic; Genes, Viral; Gentamicins; Introns; Moloney murine leukemia virus; Poly A; Proviruses; Retroviridae Proteins; RNA Splicing; RNA, Viral; Viral Envelope Proteins | 1988 |
Expression and rescue of a nonselected marker from an integrated AAV vector.
Topics: Acetyltransferases; Adenoviridae Infections; Cell Line; Chloramphenicol O-Acetyltransferase; Chromosome Mapping; Dependovirus; DNA Restriction Enzymes; Gene Expression Regulation; Genetic Vectors; Gentamicins; Humans; Promoter Regions, Genetic; Recombination, Genetic; Selection, Genetic; Viral Proteins; Virus Replication | 1988 |
Increased expression of CD4 molecules on Jurkat cells mediated by human immunodeficiency virus tat protein.
Topics: Antibodies, Monoclonal; Antigens, Differentiation, T-Lymphocyte; Cells, Cultured; Chloramphenicol O-Acetyltransferase; Cloning, Molecular; Drug Resistance; Fluorescent Antibody Technique; Gene Expression Regulation; Gene Products, tat; Genes, Viral; Genetic Vectors; Gentamicins; HIV; Humans; Moloney murine leukemia virus; RNA, Messenger; RNA, Viral; T-Lymphocytes; tat Gene Products, Human Immunodeficiency Virus; Transcription Factors | 1988 |
Metabolic correction of defects in the lipid anchoring of Thy-1 in lymphoma mutants.
Topics: Animals; Anti-Bacterial Agents; Antigens, Surface; Cell Line; Cell Membrane; Gentamicins; Glycosylation; Lymphoma; Membrane Lipids; Mutation; Phospholipids; Protein Processing, Post-Translational; RNA, Messenger; Thy-1 Antigens | 1988 |
Development of neomycin-resistant WEHI-3B D+ murine cells as an in vivo model of acute nonlymphocytic leukemia.
Topics: Animals; Blotting, Southern; Cell Differentiation; Cell Line; Clone Cells; Disease Models, Animal; Drug Resistance; Female; Gentamicins; Leukemia, Myeloid, Acute; Mice; Mice, Inbred BALB C; Neomycin; Neoplasm Transplantation; Phenotype; Transfection | 1989 |
Suppression of a nonsense mutation in mammalian cells in vivo by the aminoglycoside antibiotics G-418 and paromomycin.
Topics: Acetyltransferases; Animals; Anti-Bacterial Agents; Cell Line; Cell Survival; Chloramphenicol O-Acetyltransferase; Chlorocebus aethiops; DNA Restriction Enzymes; Gentamicins; Kidney; Kinetics; Mutation; Paromomycin; Plasmids; Suppression, Genetic; Transfection | 1985 |
Stable transformation of Drosophila Kc cells to antibiotic resistance with the bacterial neomycin resistance gene.
Topics: Animals; Anti-Bacterial Agents; Base Sequence; Cell Line; DNA; DNA Restriction Enzymes; Drosophila; Drug Resistance; Drug Resistance, Microbial; Gentamicins; Kanamycin Kinase; Neomycin; Nucleic Acid Hybridization; Phosphotransferases; Plasmids; Transfection | 1986 |
Polyoma virus middle T gene can trigger malignant transformation of early passage rodent cells.
Topics: Animals; Anti-Bacterial Agents; Antigens, Polyomavirus Transforming; Antigens, Viral, Tumor; Cell Line; Cell Transformation, Neoplastic; Cell Transformation, Viral; Cricetinae; DNA, Recombinant; Drug Resistance; Genes, Viral; Gentamicins; Lung; Oncogene Proteins, Viral; Oncogenes; Polyomavirus; Transfection | 1986 |
The myeloproliferative sarcoma virus retains transforming functions after introduction of a dominant selectable marker gene.
Topics: Animals; Cell Transformation, Neoplastic; Cell Transformation, Viral; Defective Viruses; DNA, Viral; Drug Resistance; Fibroblasts; Friend murine leukemia virus; Genes, Dominant; Genes, Viral; Genetic Vectors; Gentamicins; Mice; Moloney murine leukemia virus; Moloney murine sarcoma virus; Oncogenes; Rats; Sarcoma Viruses, Murine; Selection, Genetic; Virus Cultivation | 1986 |
Suppression of the hypomethylated Moloney leukemia virus genome in undifferentiated teratocarcinoma cells and inefficiency of transformation by a bacterial gene under control of the long terminal repeat.
Topics: Animals; Cell Differentiation; Cell Line; Drug Resistance; Gene Expression Regulation; Genes, Bacterial; Genes, Viral; Gentamicins; Kanamycin Kinase; Methylation; Mice; Moloney murine leukemia virus; Phosphotransferases; Plasmids; Promoter Regions, Genetic; Recombinant Proteins; Teratoma; Tetrahydrofolate Dehydrogenase | 1985 |
Neomycin resistance as a dominant selectable marker for selection and isolation of vaccinia virus recombinants.
Topics: Animals; Anti-Bacterial Agents; Cell Line; Chlorocebus aethiops; DNA Replication; DNA Restriction Enzymes; Drug Resistance, Microbial; Genes, Dominant; Genes, Viral; Gentamicins; Kidney; Neomycin; Plasmids; Recombination, Genetic; Vaccinia virus; Viral Plaque Assay; Virus Replication | 1985 |
A temperature-sensitive mutant of the myeloproliferative sarcoma virus, altered by a point mutation in the mos oncogene, has been modified as a selectable retroviral vector.
Topics: Base Sequence; Drug Resistance; Genetic Vectors; Gentamicins; Mutation; Oncogenes; Sarcoma Viruses, Murine; Temperature | 1987 |
Stable integration and expression of a bacterial gene in the mosquito Anopheles gambiae.
Topics: Anopheles; DNA Transposable Elements; DNA, Bacterial; Drosophila melanogaster; Drug Resistance; Female; Genes, Bacterial; Gentamicins; Male; Microinjections; Plasmids; Transformation, Genetic | 1987 |
The allosuppressor gene SAL4 encodes a protein important for maintaining translational fidelity in Saccharomyces cerevisiae.
Topics: Anti-Bacterial Agents; Blotting, Northern; Blotting, Southern; Cycloheximide; DNA Probes; DNA, Fungal; Fungal Proteins; Genes, Fungal; Gentamicins; Hygromycin B; Nucleic Acid Hybridization; Paromomycin; Phenotype; Protein Biosynthesis; Restriction Mapping; RNA, Fungal; Saccharomyces cerevisiae; Suppression, Genetic | 1988 |
Recombinant retrovirus-mediated gene transfer to normal haemopoietic cells.
Topics: Animals; Anti-Bacterial Agents; Cells, Cultured; Colony-Forming Units Assay; Colony-Stimulating Factors; Drug Resistance, Microbial; Genes, Viral; Gentamicins; Granulocyte-Macrophage Colony-Stimulating Factor; Growth Substances; Hematopoietic Stem Cells; Mice; Mice, Inbred Strains; Retroviridae; Transfection | 1988 |
Retinoic acid-induced differentiation of retrovirus-infected HL-60 cells is associated with enhanced transcription from the viral long terminal repeat.
Topics: Blotting, Northern; Cell Differentiation; Cell Line; Drug Resistance; Gene Expression Regulation; Genes, Viral; Genetic Vectors; Gentamicins; Humans; Moloney murine leukemia virus; Retroviridae; Transcription, Genetic; Tretinoin | 1988 |
Microinjection of DNA into Aedes triseriatus ova and detection of integration.
Topics: Aedes; Animals; Anti-Bacterial Agents; Blotting, Southern; DNA; Drug Resistance; Genetic Vectors; Gentamicins; Larva; Microinjections; Ovum; Plasmids; Restriction Mapping; Transformation, Genetic | 1988 |
DNA-mediated transformation in the aquatic filamentous fungus Achlya ambisexualis.
Topics: Chytridiomycota; Culture Media; DNA, Fungal; Drug Resistance, Microbial; Gentamicins; Immunoblotting; Oomycetes; Plasmids; Protoplasts; Transformation, Genetic | 1988 |
High efficiency gene transfer and expression in normal murine B lymphocytes.
Topics: Animals; B-Lymphocytes; Cell Differentiation; Drug Resistance, Microbial; Enhancer Elements, Genetic; Female; Gene Expression Regulation; Genetic Vectors; Gentamicins; Hematopoietic Stem Cells; Hemolytic Plaque Technique; Immunoglobulins; Lipopolysaccharides; Mice; Mice, Inbred CBA; Neomycin; Retroviridae; Transduction, Genetic | 1987 |
High-efficiency gene transfer and expression in normal human hematopoietic cells with retrovirus vectors.
Topics: Drug Resistance, Microbial; Gene Expression Regulation; Genetic Vectors; Gentamicins; Hematopoietic Stem Cells; Humans; Retroviridae; Transfection | 1988 |
Regional assignment of the gene for diphtheria toxin sensitivity using subchromosomal fragments in microcell hybrids.
Topics: Animals; Chromosome Mapping; Chromosomes, Human, Pair 22; Chromosomes, Human, Pair 5; Diphtheria Toxin; Drug Resistance; Gentamicins; Humans; Hybrid Cells; Mice | 1987 |
Retroviral gene transfer into primary hepatocytes: implications for genetic therapy of liver-specific functions.
Topics: Animals; Drug Resistance, Microbial; Gentamicins; Liver; Mice; Neomycin; Phenotype; Phenylalanine Hydroxylase; Retroviridae; Transfection | 1987 |
Sequence homology requirements for intermolecular recombination in mammalian cells.
Topics: Animals; Base Sequence; Chlorocebus aethiops; Drug Resistance; Drug Resistance, Microbial; Escherichia coli; Fibroblasts; Gentamicins; Humans; Plasmids; Recombination, Genetic; Sequence Homology, Nucleic Acid | 1986 |
Transformation of Phycomyces blakesleeanus to G-418 resistance by an autonomously replicating plasmid.
Topics: Drug Resistance, Microbial; Genetic Vectors; Gentamicins; Mucorales; Phycomyces; Plasmids; Replicon; Saccharomyces cerevisiae; Transformation, Genetic | 1986 |
Pleiotropic changes in cycloheximide-resistant insect cell clones.
Topics: Aedes; Animals; Anti-Bacterial Agents; Cell Division; Centrifugation, Density Gradient; Clone Cells; Cycloheximide; Drug Resistance; Gentamicins; Karyotyping; Mutation; Ribosomal Proteins; Temperature | 1987 |
Aminoglycoside antibiotic treatment of human fibroblasts: intracellular accumulation, molecular changes and the loss of ribosomal accuracy.
Topics: Anti-Bacterial Agents; Biological Transport; Cell Line; Endocytosis; Fibroblasts; Gentamicins; Humans; Kinetics; Paromomycin; Proteins; Ribosomes | 1987 |
Optimal parameters for the polybrene-induced DNA transfection of diploid human fibroblasts.
Topics: Cells, Cultured; Dimethyl Sulfoxide; DNA, Bacterial; Drug Resistance; Fibroblasts; Gentamicins; Hexadimethrine Bromide; Humans; Male; Penis; Plasmids; Polyamines; Selection, Genetic; Transformation, Genetic | 1986 |
Autofluorescence and ageing: changes in ribosome accuracy and lysosome [corrected] function.
Topics: Aging; Anti-Bacterial Agents; Cell Line; Feedback; Fluorescence; Gentamicins; Humans; Lysosomes; Mitosis; Paromomycin; Ribosomes; Streptomycin | 1986 |
Fate and biological activity of exogenous DNA sequences during serial transfections in NIH/3T3 cells.
Topics: Animals; Cell Line; DNA, Bacterial; DNA, Recombinant; Drug Resistance, Microbial; Fibroblasts; Gene Amplification; Gene Expression Regulation; Genes, Bacterial; Genetic Techniques; Gentamicins; Mice; Neoplasm Transplantation; Neoplasms, Experimental; Oncogenes; Plasmids; Transformation, Genetic | 1985 |
Il-3-dependent mouse clones that express B-220 surface antigen, contain Ig genes in germ-line configuration, and generate B lymphocytes in vivo.
Topics: Animals; Anti-Bacterial Agents; Antigens, Surface; B-Lymphocytes; Cell Differentiation; Cell Division; Clone Cells; Dexamethasone; Female; Genes; Gentamicins; Immunoglobulin Heavy Chains; Immunoglobulin kappa-Chains; Immunoglobulin Light Chains; Immunoglobulin mu-Chains; Interleukin-3; Lipopolysaccharides; Lymphocyte Activation; Lymphokines; Male; Mice; Mice, Inbred BALB C; Mice, Inbred CBA | 1985 |
Pleiotropic mutants of NIH 3T3 cells with altered regulation in the expression of both type I collagen and fibronectin.
Topics: Animals; Cell Line; Cell Transformation, Neoplastic; Collagen; Drug Resistance; Fibronectins; Gene Expression Regulation; Gentamicins; Mice; Mutation; Oncogenes; Promoter Regions, Genetic; Transfection | 1985 |
Use of gene transfer and a novel cosmid rescue strategy to isolate transforming sequences.
Topics: Animals; Cell Transformation, Neoplastic; Cosmids; DNA, Neoplasm; Drug Resistance; Gentamicins; Lung Neoplasms; Mice; Neoplasms, Experimental; Oncogenes; Transfection; Transformation, Genetic | 1985 |
Skin and mucous membrane ulceration in beagle dogs following oral dosing with an experimental aminoglycoside antibiotic.
Topics: Aminoglycosides; Animals; Anti-Bacterial Agents; Body Weight; Dogs; Female; Gentamicins; Male; Mouth Mucosa; Skin Ulcer; Stomatitis; Time Factors | 1985 |
G-418, an elongation inhibitor of 80 S ribosomes.
Topics: Animals; Anti-Bacterial Agents; Gentamicins; Kinetics; Liver; Peptide Chain Elongation, Translational; Plants; Poly A; Protein Biosynthesis; Rats; Ribosomes; RNA; RNA, Messenger; Triticum | 1983 |
A new dominant hybrid selective marker for higher eukaryotic cells.
Topics: Animals; Anti-Bacterial Agents; Cell Line; Cell Transformation, Viral; DNA Transposable Elements; Drug Resistance, Microbial; Genetic Markers; Gentamicins; Haplorhini; Humans; Kanamycin Kinase; Mice; Molecular Weight; Operon; Phosphotransferases; Plasmids; Simplexvirus; Thymidine Kinase; Transfection | 1981 |
A stable bovine papillomavirus hybrid plasmid that expresses a dominant selective trait.
Topics: Animals; Bovine papillomavirus 1; Cell Transformation, Viral; DNA Transposable Elements; Drug Resistance, Microbial; Genes, Viral; Genetic Markers; Gentamicins; Mice; Neomycin; Papillomaviridae; Phenotype; Plasmids | 1983 |
Efficient transformation of Dictyostelium discoideum amoebae.
Topics: Anti-Bacterial Agents; Cycloheximide; Dictyostelium; DNA Restriction Enzymes; Drug Resistance; Escherichia coli; Genes; Genes, Bacterial; Gentamicins; Kanamycin Kinase; Operon; Phosphotransferases; Plasmids; Simplexvirus; Thymidine Kinase; Transformation, Genetic | 1983 |
Direct selection of Saccharomyces cerevisiae resistant to the antibiotic G418 following transformation with a DNA vector carrying the kanamycin-resistance gene of Tn903.
Topics: DNA, Recombinant; Drug Resistance, Microbial; Escherichia coli; Genes, Fungal; Genetic Vectors; Gentamicins; Kanamycin; Plasmids; Saccharomyces cerevisiae; Transformation, Genetic | 1983 |
Somatic cell hybrid selection with a transfectable dominant marker.
Topics: Animals; Anti-Bacterial Agents; Cell Line; Drug Resistance; Genes, Dominant; Genetic Vectors; Gentamicins; Humans; Hybrid Cells; Hypoxanthine Phosphoribosyltransferase; Mice; Neomycin; Plasmacytoma; Plasmids; Transfection | 1984 |
Susceptibility of Saccharomyces spp. and Schwanniomyces spp. to the aminoglycoside antibiotic G418.
Topics: Anti-Bacterial Agents; Ascomycota; Drug Resistance, Microbial; Gentamicins; Microbial Sensitivity Tests; Saccharomyces; Saccharomyces cerevisiae; Saccharomycetales | 1984 |
Use of plasmid-mediated resistance to the antibiotic G418 for the rapid screening of a yeast mutant library.
Topics: Anti-Bacterial Agents; Drug Resistance, Microbial; Escherichia coli; Gentamicins; Hot Temperature; Kanamycin Resistance; Mutation; Plasmids; Saccharomyces cerevisiae; Transformation, Genetic | 1984 |
DNA transformation of a pluripotent mouse embryonal stem cell line with a dominant selective marker.
Topics: Animals; Blastocyst; Cell Differentiation; Cell Separation; Clone Cells; DNA; Drug Resistance; Genes, Dominant; Genetic Markers; Gentamicins; Hybridization, Genetic; Mice; Stem Cells; Teratoma; Transformation, Genetic | 1984 |
Selective elimination of fibroblasts from cultures of normal human melanocytes.
Topics: Cell Division; Cell Separation; Culture Media; Culture Techniques; Fibroblasts; Gentamicins; Humans; Kinetics; Melanocytes | 1984 |
A new antibiotic with known resistance factors, G418, inhibits plant cells.
Topics: Cell Division; Drug Resistance; Gentamicins; Nicotiana; Plants; Plants, Toxic | 1981 |
The expression of biologically active human p53 in Leishmania cells: a novel eukaryotic system to produce recombinant proteins.
Topics: Animals; Anti-Bacterial Agents; Base Sequence; Cytoplasm; DNA; Drug Resistance; Electroporation; Enhancer Elements, Genetic; Escherichia coli; Genetic Vectors; Gentamicins; Humans; Leishmania donovani; Molecular Sequence Data; Phosphorylation; Protein Conformation; Recombinant Proteins; RNA, Messenger; Tumor Suppressor Protein p53 | 1995 |
Adeno-associated virus 2-mediated high efficiency gene transfer into immature and mature subsets of hematopoietic progenitor cells in human umbilical cord blood.
Topics: Antigens, CD; Antigens, CD34; Base Sequence; Blotting, Southern; Cell Division; Colony-Forming Units Assay; Cytokines; Dependovirus; DNA; DNA Primers; Drug Resistance, Microbial; Female; Fetal Blood; Gene Transfer Techniques; Genes, Bacterial; Genetic Vectors; Gentamicins; Hematopoietic Stem Cells; Humans; Molecular Sequence Data; Neomycin; Polymerase Chain Reaction; Pregnancy; Promoter Regions, Genetic; Thymidine Kinase; Virion | 1994 |
Expression of genes carried by pR plasmid in damaged E. coli and mouse cells.
Topics: 4-Nitroquinoline-1-oxide; Animals; Bacterial Proteins; Bleomycin; Cell Line; Cisplatin; DNA Damage; DNA Repair; DNA Transposable Elements; DNA-Binding Proteins; Escherichia coli; Escherichia coli Proteins; Gene Expression; Gentamicins; Methylnitronitrosoguanidine; Mice; Mitomycin; Mutagens; Plasmids; RNA, Messenger; Transcription, Genetic; Ultraviolet Rays | 1994 |
Transfection and continuous expression of heterologous genes in the protozoan parasite Entamoeba histolytica.
Topics: Animals; Bacterial Proteins; Base Sequence; Chloramphenicol O-Acetyltransferase; DNA Replication; Drug Resistance; Entamoeba histolytica; Gene Dosage; Gene Expression; Genes, Bacterial; Genetic Vectors; Gentamicins; Kanamycin Kinase; Molecular Sequence Data; Phosphotransferases (Alcohol Group Acceptor); Plasmids; Recombinant Proteins; Transfection | 1995 |
Immunological screening of homologous recombination in genes that encode surface antigens.
Topics: Animals; Base Sequence; Cytotoxicity Tests, Immunologic; Drug Resistance; Ganciclovir; Gene Targeting; Genes, MHC Class I; Gentamicins; Mice; Molecular Sequence Data; Polymerase Chain Reaction; Recombination, Genetic; Tumor Cells, Cultured | 1995 |
Stable production of mutant mice from double gene converted ES cells with puromycin and neomycin.
Topics: Acetyltransferases; Actins; Animals; Base Sequence; Cell Division; Chickens; Chimera; DNA Primers; Embryo, Mammalian; Embryo, Nonmammalian; Female; Genes, myc; Gentamicins; Humans; Male; Mice; Mice, Inbred Strains; Mice, Mutant Strains; Mice, Transgenic; Molecular Sequence Data; Neomycin; Polymerase Chain Reaction; Promoter Regions, Genetic; Proto-Oncogene Proteins; Proto-Oncogene Proteins c-fyn; Proto-Oncogenes; Puromycin; Stem Cells | 1995 |
Reporter gene expression upon stable transfection when only a TATA box or a TATA box plus Sp1 sites are present 5' to the gene.
Topics: Animals; Base Sequence; Cinnamates; Drug Resistance; Genes, Reporter; Genetic Vectors; Gentamicins; HeLa Cells; Humans; Hygromycin B; Kanamycin Kinase; Mice; Molecular Sequence Data; Phosphotransferases (Alcohol Group Acceptor); Plasmids; Sp1 Transcription Factor; TATA Box; Transcription, Genetic; Transfection; Tumor Cells, Cultured | 1995 |
Disruption of the adenosine deaminase (ADA) gene using a dicistronic promoterless construct: production of an ADA-deficient homozygote ES cell line.
Topics: Adenosine Deaminase; Animals; Base Sequence; beta-Galactosidase; Blotting, Southern; Cell Line; Exons; Fluoresceins; Galactosides; Gene Targeting; Gentamicins; Homozygote; Kanamycin Kinase; Mice; Models, Genetic; Molecular Sequence Data; Mutagenesis, Insertional; Phosphotransferases (Alcohol Group Acceptor); Protein Biosynthesis; Stem Cells | 1995 |
Human IgE mediates stimulus secretion coupling in rat basophilic leukemia cells transfected with the alpha chain of the human high-affinity receptor.
Topics: Adenosine; Adenosine-5'-(N-ethylcarboxamide); Animals; Dexamethasone; Gentamicins; Histamine Release; Humans; Immunoglobulin E; Leukemia, Basophilic, Acute; Rats; Receptors, IgE; Serotonin; Transfection; Tumor Cells, Cultured | 1993 |
Tumor suppressor function of the interferon-induced double-stranded RNA-activated protein kinase.
Topics: 3T3 Cells; Amino Acid Sequence; Animals; Cell Transformation, Neoplastic; eIF-2 Kinase; Enzyme Induction; Female; Gene Expression; Gentamicins; Humans; Interferons; Lysine; Mice; Molecular Weight; Mutagenesis, Site-Directed; Neoplasms, Experimental; Protein Kinases; Recombinant Proteins; Restriction Mapping; Transfection; Tumor Cells, Cultured | 1993 |
[Agents that act on chromatin structure affect the rate of intrachromosomal homologous DNA recombination in cultured cells].
Topics: Animals; Butyrates; Butyric Acid; Cell Line; Chromatin; Chromosomes; Clone Cells; Cricetinae; Cricetulus; Cyclic AMP; Dexamethasone; DNA; Drug Resistance; Gentamicins; Kanamycin Kinase; Luminol; Phosphotransferases (Alcohol Group Acceptor); Plasmids; Recombination, Genetic; Sequence Homology, Nucleic Acid | 1994 |
Selection of glucocorticoid-resistant mutations from an AtT-20 cell line containing a glucocorticoid-regulated selectable transgene.
Topics: Animals; Cell Division; Dexamethasone; Drug Resistance; Gene Expression Regulation; Gentamicins; Kanamycin Kinase; Mice; Mutation; Phosphotransferases (Alcohol Group Acceptor); Receptors, Glucocorticoid; RNA, Messenger; Tumor Cells, Cultured | 1995 |
A new retroviral vector for detecting mutations and chromosomal instability in mammalian cells.
Topics: Acyclovir; Alpharetrovirus; Animals; Base Sequence; Cells, Cultured; Dose-Response Relationship, Radiation; Fibroblasts; Genetic Vectors; Gentamicins; Molecular Sequence Data; Mutagenesis; Mutagenicity Tests; Rats; Selection, Genetic; Sequence Analysis, DNA; Simplexvirus; Thymidine Kinase; Ultraviolet Rays | 1995 |
Rapid selection using G418 of high copy number transformants of Pichia pastoris for high-level foreign gene expression.
Topics: Base Sequence; Blotting, Northern; Blotting, Western; Cloning, Molecular; Drug Resistance, Microbial; Gene Expression; Gene Products, env; Genes, Fungal; Genetic Vectors; Gentamicins; HIV Envelope Protein gp120; HIV-1; Molecular Sequence Data; Mutagenesis, Site-Directed; Pichia; Promoter Regions, Genetic; Recombinant Proteins; Restriction Mapping; RNA, Messenger; Transformation, Genetic | 1994 |
Analysis of optimal conditions for retroviral-mediated transduction of primitive human hematopoietic cells.
Topics: Animals; Blood Cells; Bone Marrow Cells; Cell Survival; Cells, Cultured; Chimera; Colony-Forming Units Assay; Connective Tissue; Culture Techniques; Drug Resistance; Genes, Reporter; Genetic Vectors; Gentamicins; Graft Survival; Hematopoietic Cell Growth Factors; Hematopoietic Stem Cell Transplantation; Hematopoietic Stem Cells; Humans; Immunologic Deficiency Syndromes; Kanamycin Kinase; Mice; Mice, Mutant Strains; Mice, Nude; Phosphotransferases (Alcohol Group Acceptor); Polymerase Chain Reaction; Proviruses; Recombinant Proteins; Retroviridae; Transfection; Transplantation, Heterologous | 1995 |
A simple cytotoxicity assay using the eucaryotic microorganism Dictyostelium discoideum.
Topics: Animals; Cytotoxins; Dictyostelium; Doxorubicin; Gentamicins; Models, Biological; Toxicity Tests | 1994 |
A copy-number-controlled expression vector for the fission yeast Schizosaccharomyces pombe.
Topics: Annexin A1; Base Sequence; Blotting, Northern; Blotting, Southern; Chloramphenicol O-Acetyltransferase; Cloning, Molecular; Cytomegalovirus; Drug Resistance; Enhancer Elements, Genetic; Gene Expression; Genetic Vectors; Gentamicins; Humans; Kinetics; Molecular Sequence Data; Neomycin; Oligodeoxyribonucleotides; Promoter Regions, Genetic; Recombinant Proteins; Schizosaccharomyces; Simian virus 40; Transcription, Genetic | 1994 |
Characterization of primary cultures of chondrocytes from type II collagen/beta-galactosidase transgenic mice.
Topics: Animals; Bacterial Proteins; beta-Galactosidase; Cells, Cultured; Collagen; Drug Resistance; Escherichia coli; Gene Expression Regulation; Genes, Reporter; Genes, Synthetic; Gentamicins; Growth Plate; Kanamycin Kinase; Mice; Mice, Transgenic; Neomycin; Phenotype; Phosphotransferases (Alcohol Group Acceptor); Recombinant Fusion Proteins | 1994 |
Transfer of an anti-HIV-1 ribozyme gene into primary human lymphocytes.
Topics: Base Sequence; Cells, Cultured; Drug Resistance; Gene Transfer Techniques; Genetic Vectors; Gentamicins; HIV-1; HIV-2; Humans; Immunity, Innate; Lymphocytes; Molecular Sequence Data; Recombinant Fusion Proteins; RNA Polymerase III; RNA, Catalytic; Selection, Genetic; Virus Replication | 1994 |
Analysis of a complex plasmid insertion in a phototaxis-deficient transformant of Dictyostelium discoideum selected on a Micrococcus luteus lawn.
Topics: Animals; Base Sequence; Cell Movement; Dictyostelium; DNA, Fungal; DNA, Protozoan; Drug Resistance, Microbial; Genetic Vectors; Gentamicins; Kanamycin Kinase; Light; Micrococcus luteus; Molecular Sequence Data; Mutagenesis, Insertional; Phosphotransferases (Alcohol Group Acceptor); Selection, Genetic; Transformation, Genetic | 1994 |
Glycan requirements of glycosylphosphatidylinositol phospholipase C from Trypanosoma brucei. Glucosaminylinositol derivatives inhibit phosphatidylinositol phospholipase C.
Topics: Animals; Carbohydrate Sequence; Enzyme Activation; Gentamicins; Glycosylphosphatidylinositol Diacylglycerol-Lyase; Glycosylphosphatidylinositols; Lipid Metabolism; Molecular Sequence Data; Phosphatidylinositol Diacylglycerol-Lyase; Phosphoinositide Phospholipase C; Phosphoric Diester Hydrolases; Polysaccharides; Trypanosoma brucei brucei | 1995 |
Membrane-bound neomycin phosphotransferase confers drug-resistance in mammalian cells: a marker for high-efficiency targeting of genes encoding secreted and cell-surface proteins.
Topics: Amino Acid Sequence; Base Sequence; Biomarkers; Cells, Cultured; DNA Primers; Drug Resistance; Gene Targeting; Genetic Vectors; Gentamicins; Kanamycin Kinase; Membrane Proteins; Molecular Sequence Data; Phosphotransferases (Alcohol Group Acceptor); Plasmids; Polymerase Chain Reaction; Recombinant Fusion Proteins | 1994 |
Stable expression of the neuronal BI (class A) calcium channel in baby hamster kidney cells.
Topics: Animals; Base Sequence; Calcium Channel Blockers; Calcium Channels; Cell Line; Clone Cells; Cricetinae; DNA Primers; Gene Expression; Genetic Markers; Gentamicins; Kidney; Kinetics; Methotrexate; Molecular Sequence Data; Muscles; Neurons; Nifedipine; omega-Agatoxin IVA; Plasmids; Polymerase Chain Reaction; Restriction Mapping; Spider Venoms; Tetrahydrofolate Dehydrogenase; Transfection | 1994 |
Retroviral mediated gene transfer in chronic myelogenous leukaemia.
Topics: Base Sequence; Cytokines; Drug Resistance; Gene Transfer Techniques; Genetic Vectors; Gentamicins; Humans; Leukemia, Myelogenous, Chronic, BCR-ABL Positive; Molecular Sequence Data; Neoplastic Stem Cells; Polymerase Chain Reaction; Retroviridae; Time Factors; Tumor Cells, Cultured | 1994 |
Mutational analysis of the translational signal in the human cytomegalovirus gpUL4 (gp48) transcript leader by retroviral infection.
Topics: 3T3 Cells; Animals; Base Sequence; Cells, Cultured; Cytomegalovirus; DNA Mutational Analysis; DNA Primers; Genetic Vectors; Gentamicins; Humans; Mice; Molecular Sequence Data; Protein Biosynthesis; Retroviridae; RNA, Messenger; RNA, Viral; Signal Transduction; Transduction, Genetic; Viral Envelope Proteins | 1994 |
Parasexual crosses and hybrid selection with a near haploid variant of the epithelioma papulosum cyprini, cell line EPC: a tissue culture model for the analyses of dominance, recessiveness, and complementation of mutant phenotypes.
Topics: Animals; Carps; Cell Division; Cell Fusion; Cell Line; Clone Cells; Cloning, Molecular; Contact Inhibition; Crosses, Genetic; Drug Resistance; Genes, Dominant; Genes, Recessive; Genetic Complementation Test; Gentamicins; Hygromycin B; Karyotyping; Mutagenesis, Insertional; Phenotype; Plasmids; Transfection | 1994 |
Leishmania major HEXBP deletion mutants generated by double targeted gene replacement.
Topics: Alleles; Amino Acid Sequence; Animals; Base Sequence; DNA-Binding Proteins; Drug Resistance; Genes, Protozoan; Genetic Markers; Gentamicins; Hygromycin B; Leishmania major; Metalloendopeptidases; Mice; Mice, Inbred BALB C; Molecular Sequence Data; Multigene Family; Mutagenesis; Protozoan Proteins; Recombination, Genetic; Sequence Deletion; Zinc Fingers | 1994 |
v-src transformation of rat embryo fibroblasts. Inefficient conversion to anchorage-independent growth involves heterogeneity of primary cultures.
Topics: Actins; Animals; Cell Adhesion; Cell Division; Cell Fusion; Cell Line; Cell Line, Transformed; Cell Transformation, Neoplastic; Cell Transformation, Viral; Drug Resistance; Fibroblasts; Genes, src; Gentamicins; Hybrid Cells; Oncogene Protein pp60(v-src); Protein-Tyrosine Kinases; Rats; Retroviridae | 1994 |
Ganciclovir treatment of herpes simplex thymidine kinase-transduced primary T lymphocytes: an approach for specific in vivo donor T-cell depletion after bone marrow transplantation?
Topics: Bone Marrow; Cell Division; Cells, Cultured; Dose-Response Relationship, Drug; Ganciclovir; Gentamicins; Humans; Interleukin-2; Kinetics; Lymphocyte Activation; Lymphocyte Depletion; Simplexvirus; T-Lymphocytes; Thymidine Kinase; Time Factors; Transduction, Genetic; Transfection | 1994 |
The aminoglycoside G418 suppresses muscarinic receptor-activated calcium release in stably transfected murine N1E-115 neuroblastoma cells.
Topics: Animals; Bradykinin; Calcium; Carbachol; Gentamicins; Histamine; Mice; Neuroblastoma; Osmolar Concentration; Receptors, Muscarinic; Transfection; Tumor Cells, Cultured | 1994 |
Cellular toxicity of aminoglycoside antibiotics in G418-sensitive and -resistant LLC-PK1 cells.
Topics: Alkaline Phosphatase; Animals; Anti-Bacterial Agents; Cell Line; Cycloheximide; Drug Resistance, Microbial; Gentamicins; Kanamycin Kinase; Kidney; Leucine; Microsomes; Phosphotransferases (Alcohol Group Acceptor); Plasmids; Swine; Transfection | 1994 |
A comparative evaluation of three transfection procedures as assessed by resistance to G418 conferred to HEPG2 cells.
Topics: Animals; Calcium Phosphates; Cloning, Molecular; DNA; DNA, Circular; Drug Resistance; Electroporation; Genes, MHC Class I; Gentamicins; Humans; Kanamycin Kinase; Mice; Phosphotransferases (Alcohol Group Acceptor); Plasmids; Transfection; Tumor Cells, Cultured | 1994 |
Two more independent selectable markers for stable transfection of Leishmania.
Topics: Animals; Base Sequence; DNA, Protozoan; Drug Resistance; Genes, Protozoan; Genetic Markers; Genetic Vectors; Gentamicins; Hygromycin B; Leishmania major; Molecular Sequence Data; Phleomycins; Puromycin; Transfection | 1993 |
Transcriptional regulation of the human H ferritin-encoding gene (FERH) in G418-treated cells: role of the B-box-binding factor.
Topics: Animals; Base Sequence; Blotting, Northern; Cell Line; DNA-Binding Proteins; Ferritins; Gene Expression Regulation; Gentamicins; Humans; Molecular Sequence Data; Promoter Regions, Genetic; Rats; RNA, Messenger; Sequence Homology, Nucleic Acid; Transcription, Genetic | 1994 |
Generation of random internal deletion derivatives of YACs by homologous targeting to Alu sequences.
Topics: Animals; Cell Fusion; Chromosomes, Artificial, Yeast; DNA; Drug Resistance, Microbial; Gene Deletion; Genetic Markers; Gentamicins; Humans; Mammals; Multigene Family; Plasmids; Recombination, Genetic; Repetitive Sequences, Nucleic Acid; Restriction Mapping; Sequence Deletion | 1994 |
S-adenosyl-L-methionine decarboxylase of Acanthamoeba castellanii (Neff): purification and properties.
Topics: Acanthamoeba; Adenosylmethionine Decarboxylase; Amino Acid Sequence; Animals; Cell Division; Enzyme Stability; Gentamicins; Hydrogen-Ion Concentration; Isoelectric Point; Kinetics; Molecular Sequence Data; Molecular Weight; Pentamidine; Pyruvates; Sequence Homology, Amino Acid | 1993 |
[Effective removal of the contaminating host fibroblasts for establishment of human-tumor cultured lines].
Topics: Aged; Animals; Carcinoma, Squamous Cell; Cell Division; Cell Separation; Culture Media; Female; Fibroblasts; Gentamicins; Humans; Male; Mice; Mice, Inbred BALB C; Middle Aged; Mouth Neoplasms; Neoplasm Transplantation; Tumor Cells, Cultured | 1993 |
Retroviral gene transduction of circulating progenitor cells in patients with metastatic breast cancer.
Topics: Base Sequence; Breast Neoplasms; DNA; Drug Resistance; Female; Gene Expression Regulation, Viral; Genetic Therapy; Genetic Vectors; Gentamicins; Humans; Molecular Sequence Data; Polymerase Chain Reaction; Retroviridae; Stem Cells; Transfection | 1993 |
Establishment of human oral-cancer cell lines (KOSC-2 and -3) carrying p53 and c-myc abnormalities by geneticin treatment.
Topics: Aged; Animals; Carcinoma, Squamous Cell; Cell Division; Chromosome Aberrations; DNA, Neoplasm; Female; Genes, myc; Genes, p53; Gentamicins; Humans; Karyotyping; Male; Mice; Mice, Inbred BALB C; Mice, Nude; Middle Aged; Mouth Neoplasms; Neoplasm Transplantation; Transplantation, Heterologous; Tumor Cells, Cultured | 1994 |
Shuttle cosmid vectors for the trypanosomatid parasite Leishmania.
Topics: Animals; Base Sequence; Cloning, Molecular; Cosmids; Deoxyribonuclease BamHI; DNA, Protozoan; DNA, Recombinant; Drug Resistance; Escherichia coli; Genes, Protozoan; Genetic Complementation Test; Genetic Markers; Genomic Library; Gentamicins; Hygromycin B; Leishmania; Molecular Sequence Data; Mutagenesis, Insertional; Species Specificity; Telomere; Transfection | 1993 |
The use of genetic manipulation of MA-10 Leydig tumor cells to demonstrate the role of mitochondrial proteins in the acute regulation of steroidogenesis.
Topics: 3',5'-Cyclic-AMP Phosphodiesterases; 4-(3-Butoxy-4-methoxybenzyl)-2-imidazolidinone; Animals; Bucladesine; Chorionic Gonadotropin; Clone Cells; Electrophoresis, Gel, Two-Dimensional; Electrophoresis, Polyacrylamide Gel; Gentamicins; Kinetics; Leydig Cell Tumor; Luteinizing Hormone; Male; Metallothionein; Mice; Mitochondria; Molecular Weight; Progesterone; Promoter Regions, Genetic; Protein Biosynthesis; Protein Kinases; Proteins; Testicular Neoplasms; Transfection; Tumor Cells, Cultured; Zinc | 1993 |
The 23-kilodalton E1 phosphoprotein of bovine papillomavirus type 1 is nonessential for stable plasmid replication in murine C127 cells.
Topics: Amino Acid Sequence; Antibodies, Viral; Base Sequence; Bovine papillomavirus 1; DNA Replication; DNA-Binding Proteins; DNA, Viral; Drug Resistance, Microbial; Gentamicins; Molecular Sequence Data; Plasmids; Precipitin Tests; RNA, Viral; Selection, Genetic; Serial Passage; Transfection; Viral Proteins; Virus Replication | 1993 |
N-linked glycosylation of the ligand-binding domain of the human urokinase receptor contributes to the affinity for its ligand.
Topics: Amino Acid Sequence; Animals; Asparagine; Binding Sites; Cross-Linking Reagents; Electrophoresis, Polyacrylamide Gel; Gentamicins; Glycosylation; Humans; Kinetics; L Cells; Ligands; Mice; Molecular Weight; Mutagenesis, Site-Directed; Phosphatidylinositol Diacylglycerol-Lyase; Phosphoric Diester Hydrolases; Point Mutation; Receptors, Cell Surface; Receptors, Urokinase Plasminogen Activator; Recombinant Proteins; Transfection; Tunicamycin; Urokinase-Type Plasminogen Activator | 1993 |
Construction of a retrotransposition indicator sequence using a neomycin resistance-encoding gene containing a functional intron.
Topics: Animals; Base Sequence; Cells, Cultured; Cricetinae; DNA Transposable Elements; Escherichia coli; Gentamicins; Introns; Kanamycin Kinase; Mesocricetus; Mice; Molecular Sequence Data; Oligodeoxyribonucleotides; Phosphotransferases; Polymerase Chain Reaction; Restriction Mapping; Retroviridae; RNA Splicing; RNA, Messenger; Transformation, Bacterial | 1993 |
Agrobacterium-mediated production of transgenic rice plants expressing a chimeric alpha-amylase promoter/beta-glucuronidase gene.
Topics: Agrobacterium tumefaciens; alpha-Amylases; Base Sequence; DNA Transposable Elements; Drug Resistance; Gene Expression Regulation, Enzymologic; Gentamicins; Glucuronidase; Kanamycin Kinase; Molecular Sequence Data; Oncogenes; Oryza; Phosphotransferases; Plants, Genetically Modified; Promoter Regions, Genetic; Recombinant Fusion Proteins; Regulatory Sequences, Nucleic Acid | 1993 |
Introduction of a point mutation into the mouse genome by homologous recombination in embryonic stem cells using a replacement type vector with a selectable marker.
Topics: Alleles; Amino Acid Sequence; Animals; Base Sequence; beta-Endorphin; Blotting, Southern; DNA; Embryo, Mammalian; Exons; Ganciclovir; Genetic Markers; Genetic Vectors; Genome; Gentamicins; Mice; Molecular Sequence Data; Mutagenesis, Insertional; Oligodeoxyribonucleotides; Point Mutation; Polymerase Chain Reaction; Pro-Opiomelanocortin; Protein Biosynthesis; Recombination, Genetic; Simplexvirus; Stem Cells; Thymidine Kinase; Tyrosine | 1993 |
Analysis of G418-selected Rat2 cells containing prototype, variant, mutant, and chimeric JC virus and SV40 genomes.
Topics: Animals; Antigens, Polyomavirus Transforming; Cell Line; Cell Transformation, Viral; Drug Resistance; Genome, Viral; Gentamicins; JC Virus; Rats; Selection, Genetic; Simian virus 40; Tumor Suppressor Protein p53; Viral Proteins; Virus Integration | 1993 |
Elevation of sister chromatid exchange frequency in transformed human fibroblasts following exposure to widely used aminoglycosides.
Topics: Cell Line, Transformed; Fibroblasts; Gentamicins; Humans; Hygromycin B; Mutagens; Regression Analysis; Sister Chromatid Exchange; Tumor Cells, Cultured | 1993 |
The complete sequences of plasmids pFNeo and pMH-Neo: convenient expression vectors for high-level expression of eukaryotic genes in hematopoietic cell lines.
Topics: Animals; Anti-Bacterial Agents; Base Sequence; Cell Line; Cloning, Molecular; Friend murine leukemia virus; Gene Expression; Genetic Vectors; Gentamicins; Hematopoiesis; Humans; Molecular Sequence Data; Oligodeoxyribonucleotides; Plasmids; Repetitive Sequences, Nucleic Acid; Restriction Mapping; Spleen Focus-Forming Viruses; Transfection | 1993 |
Studies of human uveal melanocytes in vitro: isolation, purification and cultivation of human uveal melanocytes.
Topics: Adult; Aged; Cell Division; Cell Line; Cell Separation; Cell Survival; Cells, Cultured; Culture Media; Cytoskeletal Proteins; Fibroblasts; Gentamicins; Humans; Immunoenzyme Techniques; Melanocytes; Microscopy, Phase-Contrast; Middle Aged; Pigment Epithelium of Eye; S100 Proteins; Uvea | 1993 |
New vector for transfer of yeast artificial chromosomes to mammalian cells.
Topics: Animals; Blotting, Southern; CHO Cells; Chromosomes, Fungal; Cricetinae; Drug Resistance; Gene Library; Genetic Vectors; Gentamicins; In Situ Hybridization, Fluorescence; Mammals; Transfection | 1993 |
Use of an in vivo system to determine the G418 resistance phenotype of bloodstream-form Trypanosoma brucei brucei transfectants.
Topics: Animals; Drug Resistance; Gentamicins; Microbial Sensitivity Tests; Phenotype; Plasmids; Transfection; Trypanosoma brucei brucei; Trypanosomiasis, African | 1993 |
Electroporation of human lymphoblastoid cells.
Topics: Cell Line; Drug Resistance; Electroporation; Genetic Markers; Gentamicins; Humans; Indicators and Reagents; Kanamycin Kinase; Lymphocytes; Phosphotransferases (Alcohol Group Acceptor); Plasmids; Recombination, Genetic; Transfection | 1995 |
Effect of cis-located human satellite DNA on electroporation efficiency.
Topics: Aminopterin; Cell Line, Transformed; DNA, Satellite; Drug Resistance; Electroporation; Gentamicins; Humans; Hypoxanthine; Hypoxanthines; Plasmids; Thymidine; Transfection | 1995 |
A bicistronic retrovirus vector containing a picornavirus internal ribosome entry site allows for correction of X-linked CGD by selection for MDR1 expression.
Topics: ATP Binding Cassette Transporter, Subfamily B, Member 1; B-Lymphocytes; Cell Line, Transformed; Genetic Therapy; Genetic Vectors; Gentamicins; Granulomatous Disease, Chronic; Herpesvirus 4, Human; Humans; Kanamycin Kinase; Membrane Glycoproteins; Moloney murine leukemia virus; NADPH Oxidase 2; NADPH Oxidases; Phosphotransferases (Alcohol Group Acceptor); Poliovirus; Promoter Regions, Genetic; Protein Biosynthesis; Recombinant Fusion Proteins; Regulatory Sequences, Nucleic Acid; Ribosomes; RNA, Messenger; Selection, Genetic; Transfection; Vincristine; X Chromosome | 1996 |
Aminoglycoside antibiotics restore CFTR function by overcoming premature stop mutations.
Topics: Anti-Bacterial Agents; Cystic Fibrosis; Cystic Fibrosis Transmembrane Conductance Regulator; Gentamicins; HeLa Cells; Humans; Mutation; Suppression, Genetic | 1996 |
Bicistronic and two-gene retroviral vectors for using MDR1 as a selectable marker and a therapeutic gene.
Topics: 3T3 Cells; Animals; ATP Binding Cassette Transporter, Subfamily B, Member 1; Base Sequence; Colchicine; DNA Primers; Gene Expression Regulation, Viral; Gene Transfer Techniques; Genes; Genes, Reporter; Genetic Markers; Genetic Therapy; Genetic Vectors; Gentamicins; Harvey murine sarcoma virus; Humans; KB Cells; Mice; Molecular Sequence Data; Tumor Cells, Cultured | 1996 |
Bacterial lipopolysaccharide confers resistance to G418, doxorubicin, and taxol in the murine macrophage cell line, RAW264.
Topics: Animals; Antibiotics, Antineoplastic; Antineoplastic Agents, Phytogenic; Cell Line; Cell Survival; Doxorubicin; Drug Interactions; Drug Resistance, Microbial; Drug Resistance, Neoplasm; Gentamicins; Lipopolysaccharides; Macrophages; Mice; Paclitaxel; Succinate Dehydrogenase | 1996 |
Stable expression of mosaic coats of variant surface glycoproteins in Trypanosoma brucei.
Topics: Animals; Antigenic Variation; Cell Membrane; Gentamicins; Parasitemia; Protein Synthesis Inhibitors; Rats; Transfection; Trypanosoma brucei brucei; Trypanosomiasis, African; Variant Surface Glycoproteins, Trypanosoma | 1996 |
Phosphatidylinositol phospholipase C is activated allosterically by the aminoglycoside G418. 2-deoxy-2-fluoro-scyllo-inositol-1-O-dodecylphosphonate and its analogs inhibit glycosylphosphatidylinositol phospholipase C.
Topics: Allosteric Regulation; Animals; Anti-Bacterial Agents; Bacillus cereus; Binding, Competitive; Cattle; Cells, Cultured; Concanavalin A; Enzyme Activation; Enzyme Inhibitors; Gentamicins; Glycosylphosphatidylinositols; Humans; Inositol; Kinetics; Organophosphonates; Phosphatidylinositol Diacylglycerol-Lyase; Phosphoinositide Phospholipase C; Phosphoric Diester Hydrolases; Structure-Activity Relationship; Substrate Specificity; Type C Phospholipases | 1996 |
Antisense inhibition of the 27 kDa heat shock protein production affects growth rate and cytoskeletal organization in MCF-7 cells.
Topics: Actins; Antibody Specificity; Antisense Elements (Genetics); Base Sequence; Blotting, Western; Breast Neoplasms; Cell Division; Clone Cells; Cytoskeleton; Female; Gentamicins; Heat-Shock Proteins; Humans; Molecular Sequence Data; Phenotype; Transfection; Trypsin; Tumor Cells, Cultured | 1996 |
Anti-HIV viral interference induced by retroviral vectors expressing a nonproducer HIV-1 variant.
Topics: Acquired Immunodeficiency Syndrome; Animals; Blotting, Southern; Cell Line; DNA, Antisense; Gene Expression Regulation, Viral; Gene Products, gag; Genes, nef; Genetic Therapy; Genetic Vectors; Gentamicins; HeLa Cells; HIV Long Terminal Repeat; HIV-1; Humans; Lymphocytes; Mice; Moloney murine leukemia virus; Repetitive Sequences, Nucleic Acid; Transfection; Viral Interference; Virus Integration | 1996 |
Genetically selected cardiomyocytes from differentiating embronic stem cells form stable intracardiac grafts.
Topics: Animals; Base Sequence; Biomarkers; Cell Differentiation; Genetic Engineering; Gentamicins; Kanamycin Kinase; Mice; Molecular Sequence Data; Myocardium; Myosins; Phosphotransferases (Alcohol Group Acceptor); Promoter Regions, Genetic; Selection, Genetic; Stem Cells; Tissue Transplantation | 1996 |
Molecular characterization of a novel cancer cell line established from human carcinoma in pleomorphic adenoma (CaPA-4).
Topics: Adenoma, Pleomorphic; Animals; Base Sequence; Cell Division; Fibroblasts; Gene Expression; Genes, myc; Genes, p53; Genes, ras; Gentamicins; Humans; Mice; Mice, Nude; Microscopy, Electron; Neoplasm Transplantation; Point Mutation; Submandibular Gland Neoplasms; Tumor Cells, Cultured | 1996 |
Elevated levels of recombinational DNA repair in human somatic cells expressing the Saccharomyces cerevisiae RAD52 gene.
Topics: Anti-Bacterial Agents; Blotting, Southern; Cell Line; DNA Repair; DNA-Binding Proteins; DNA, Recombinant; Fungal Proteins; Gentamicins; Humans; Phenotype; Plasmids; Polymerase Chain Reaction; Rad52 DNA Repair and Recombination Protein; RNA, Messenger; Saccharomyces cerevisiae; Saccharomyces cerevisiae Proteins; Sister Chromatid Exchange; Transfection | 1996 |
[Formation of clone cells resistant to G418 and not containing human chromosomes as a result of somatic hybridization of cell line A9 with human fibroblasts transfected with pSV2neo].
Topics: Animals; Anti-Bacterial Agents; Cell Line, Transformed; Chromosomes, Human; Clone Cells; Drug Resistance; Gentamicins; Humans; Hybrid Cells; Mice; Plasmids | 1996 |
Human repeat-mediated integration of selectable markers into somatic cell hybrids.
Topics: Anti-Bacterial Agents; Base Sequence; DNA; Drug Resistance, Microbial; Gene Targeting; Genetic Markers; Genetic Vectors; Gentamicins; Humans; Hybrid Cells; In Situ Hybridization, Fluorescence; Molecular Sequence Data; Polymerase Chain Reaction; Repetitive Sequences, Nucleic Acid; Transfection | 1995 |
Passage to nonselective media transiently alters growth of mycophenolic acid-resistant mammalian cells expressing the escherichia coli xanthine-guanine phosphoribosyltransferase gene: implications for sequential selection strategies.
Topics: 3T3 Cells; Animals; Anti-Bacterial Agents; Cell Division; Clone Cells; Drug Resistance; Escherichia coli; Gentamicins; Hygromycin B; Mice; Mycophenolic Acid; Pentosyltransferases; Protein Synthesis Inhibitors; Puromycin; Restriction Mapping; Transfection | 1996 |
Gene-directed enzyme prodrug therapy with a mustard prodrug/carboxypeptidase G2 combination.
Topics: Animals; Anti-Bacterial Agents; Antineoplastic Agents; Colonic Neoplasms; COS Cells; Drug Screening Assays, Antitumor; Female; gamma-Glutamyl Hydrolase; Genetic Therapy; Genetic Vectors; Gentamicins; Glutamates; Humans; Mutagenesis, Site-Directed; Nitrogen Mustard Compounds; Ovarian Neoplasms; Prodrugs; Transfection; Tumor Cells, Cultured | 1996 |
Selective ablation of differentiated cells permits isolation of embryonic stem cell lines from murine embryos with a non-permissive genetic background.
Topics: Animals; Anti-Bacterial Agents; Cell Culture Techniques; Cell Differentiation; Cell Line; Chimera; Crosses, Genetic; Drug Resistance; Embryo, Mammalian; Female; Gentamicins; Germ Cells; Male; Mice; Mice, Inbred C57BL; Mice, Inbred CBA; Mice, Transgenic; Stem Cells | 1996 |
Stable MLV-VL30 dicistronic retroviral vectors with a VL30 or MoMLV sequence promoting both packaging of genomic RNA and expression of the 3' cistron.
Topics: 3T3 Cells; Alkaline Phosphatase; Animals; Base Sequence; Electrophoresis, Agar Gel; Friend murine leukemia virus; Gene Expression Regulation; Gene Transfer Techniques; Genes, Viral; Genetic Vectors; Gentamicins; Humans; Kanamycin Kinase; Mice; Molecular Sequence Data; Phosphotransferases (Alcohol Group Acceptor); Placenta; Retroviridae; RNA, Messenger; RNA, Viral; Sequence Deletion; Viral Structural Proteins | 1996 |
Antifection: an antibody-mediated method to introduce genes into lymphoid cells in vitro and in vivo.
Topics: Antibodies; Antigens, CD; B-Lymphocytes; beta-Galactosidase; Binding, Competitive; Cell Line; DNA; Drug Resistance; Gentamicins; Immunoconjugates; Lymphoid Tissue; Neomycin; Receptors, Antigen, B-Cell; Spleen; Transfection | 1996 |
Effective elimination of fibroblasts in cultures of melanocytes by lowering calcium concentration in TPA depleted medium following geneticin treatment.
Topics: Anti-Bacterial Agents; Calcium; Cell Separation; Cells, Cultured; Culture Media, Serum-Free; Endothelin-1; Fibroblast Growth Factor 2; Fibroblasts; Gentamicins; Humans; Infant, Newborn; Male; Melanocytes; Tetradecanoylphorbol Acetate | 1996 |
Stromal cells maintain the radioprotective capacity of CFU-S during retroviral infection.
Topics: Animals; Anti-Bacterial Agents; Cell Adhesion; Cell Line; Coculture Techniques; Gene Transfer Techniques; Genetic Vectors; Gentamicins; Hematopoietic Stem Cell Transplantation; Hematopoietic Stem Cells; Kanamycin Kinase; Male; Mice; Mice, Inbred CBA; Phosphotransferases (Alcohol Group Acceptor); Radiation Tolerance; Retroviridae; Spleen; Stromal Cells; Virus Integration | 1996 |
Expression of Escherichia coli recA and ada genes in Saccharomyces cerevisiae using a vector with geneticin resistance.
Topics: Bacterial Proteins; Drug Resistance, Microbial; Escherichia coli; Escherichia coli Proteins; Gene Expression; Genes, Bacterial; Genetic Vectors; Genotype; Gentamicins; Mutation; O(6)-Methylguanine-DNA Methyltransferase; Plasmids; Rec A Recombinases; Recombinant Proteins; Saccharomyces cerevisiae; Transcription Factors; Transformation, Genetic | 1995 |
Clonal nature of spontaneously immortalized 3T3 cells.
Topics: 3T3 Cells; Animals; Anti-Bacterial Agents; Cell Division; Cell Line, Transformed; Cell Transformation, Viral; Clone Cells; Culture Techniques; Defective Viruses; DNA; Drug Resistance; Gentamicins; Mice; Retroviridae | 1996 |
Smooth muscle alternative splicing induced in fibroblasts by heterologous expression of a regulatory gene.
Topics: Actinin; Alternative Splicing; Animals; Blotting, Western; Cell Extracts; Cloning, Molecular; Drug Resistance; Fibroblasts; Gene Expression Regulation; Gentamicins; Muscle, Smooth; Polymerase Chain Reaction; Rats; RNA-Binding Proteins; Transfection; Tropomyosin | 1996 |
[New immortalized cell lines from transgenic rat embryos. I. The isolation and characteristics of cell lines from rat embryos with an injected v-mos oncogene coupled with or without the gene for genecitin resistance].
Topics: Animals; Animals, Genetically Modified; Anti-Bacterial Agents; Cell Line; Chromosome Deletion; Drug Resistance; Embryo, Mammalian; Genes, mos; Genotype; Gentamicins; Karyotyping; Phenotype; Plasmids; Rats; Transgenes | 1996 |
Sites and types of UV-induced mutations leading to inactivation of the growth-arresting activity in p21 (sdi1/cip1/waf1) cDNA.
Topics: Animals; Anti-Bacterial Agents; Cell Division; CHO Cells; Cricetinae; Cyclin-Dependent Kinase Inhibitor p21; Cyclins; DNA; Drug Resistance; Gentamicins; Mutation; Plasmids; Transfection; Ultraviolet Rays | 1996 |
Development of a VSV-G protein pseudotyped retroviral vector system expressing dominant oncogenes from a lacO-modified inducible LTR promoter.
Topics: Antigens, Polyomavirus Transforming; Blotting, Northern; Chloramphenicol O-Acetyltransferase; Enzyme-Linked Immunosorbent Assay; Fibroblasts; Gene Expression Regulation; Genes, ras; Genes, Reporter; Genes, Viral; Genetic Vectors; Gentamicins; Humans; Isopropyl Thiogalactoside; Oncogenes; Plasmids; Retroviridae; Transformation, Genetic | 1996 |
Stable coexpression of a drug-resistance gene and a heterologous gene in an ancient parasitic protozoan Giardia lamblia.
Topics: Amebicides; Animals; Anti-Bacterial Agents; Cloning, Molecular; Drug Resistance; Gene Expression; Genes, Protozoan; Genes, Reporter; Genetic Vectors; Gentamicins; Giardia lamblia; Giardiavirus; Kanamycin Kinase; Luciferases; Paromomycin; Phosphotransferases (Alcohol Group Acceptor); Transformation, Genetic | 1996 |
The effect of transfection of antisense cDNA for procollagen alpha 1 (IV) on stimulated proliferation in rat glomerular endothelial cells.
Topics: Animals; Anti-Bacterial Agents; Blotting, Western; Cell Division; Cells, Cultured; Densitometry; DNA, Antisense; DNA, Complementary; Endothelium; Gentamicins; Kidney Glomerulus; Microscopy, Fluorescence; Procollagen; Rats; Rats, Sprague-Dawley; Transfection | 1997 |
Molecular spectrum of mutations induced by 5-hydroxymethyl-2'-deoxyuridine in (CHO)-PL61 cells.
Topics: Animals; Anti-Bacterial Agents; Bacterial Proteins; Base Sequence; Blotting, Southern; CHO Cells; Cricetinae; DNA; DNA Repair; Escherichia coli Proteins; Gene Expression Regulation; Gentamicins; Hypoxanthine Phosphoribosyltransferase; Kanamycin Kinase; Molecular Sequence Data; Mutagenesis; Mutagenesis, Insertional; Mutagenicity Tests; Pentosyltransferases; Phosphotransferases (Alcohol Group Acceptor); Point Mutation; Polymerase Chain Reaction; Polymorphism, Single-Stranded Conformational; Proteins; Sequence Deletion; Thioguanine; Thymidine | 1997 |
High copy number integration into the ribosomal DNA of the yeast Phaffia rhodozyma.
Topics: Actins; Blotting, Southern; Cloning, Molecular; DNA Probes; DNA, Ribosomal; Drug Resistance; Electrophoresis, Agar Gel; Electrophoresis, Gel, Pulsed-Field; Gene Dosage; Genes, Fungal; Genetic Markers; Gentamicins; Kanamycin; Plasmids; Promoter Regions, Genetic; Transformation, Genetic | 1997 |
A simple and rapid method for the determination of recombinant retrovirus titer by G418 selection.
Topics: 3T3 Cells; Animals; Anti-Bacterial Agents; Cell Division; Genetic Vectors; Gentamicins; Mice; Recombination, Genetic; Retroviridae | 1996 |
Development of a high-performance liquid chromatographic assay for G418 sulfate (Geneticin).
Topics: Animals; Anti-Bacterial Agents; Buffers; Chromatography, High Pressure Liquid; Dinitrofluorobenzene; Gentamicins; Liver; Mice; Spectrophotometry, Ultraviolet | 1997 |
A simple procedure to determine the biological titer of recombinant retroviral vectors.
Topics: Animals; Biological Assay; Cell Line; Drug Resistance; Galactosides; Genetic Vectors; Gentamicins; Humans; Indoles; Mice; Moloney murine leukemia virus; Time Factors; Transduction, Genetic | 1997 |
Addition of G418 and other aminoglycoside antibiotics to mammalian cells results in the release of GPI-anchored proteins.
Topics: Alkaline Phosphatase; Animals; Anti-Bacterial Agents; Chlorocebus aethiops; COS Cells; Dose-Response Relationship, Drug; Enzyme Activation; Erythrocytes; Gentamicins; Glycosylphosphatidylinositols; Humans; Membrane Proteins; Time Factors; Transfection | 1997 |
Long-term, stable expression of green fluorescent protein in mammalian cells.
Topics: Animals; CHO Cells; Cricetinae; Drug Resistance; Gentamicins; Green Fluorescent Proteins; Luminescent Proteins; Time Factors; Transfection | 1997 |
Deletion of the yeast homologue of the human gene associated with Friedreich's ataxia elicits iron accumulation in mitochondria.
Topics: Adenosine Triphosphatases; Animals; Cell Division; Cell Respiration; Copper; DNA, Mitochondrial; Frataxin; Friedreich Ataxia; Gene Deletion; Genes, Fungal; Gentamicins; Humans; Hydrogen Peroxide; Iron; Iron-Binding Proteins; Meiosis; Mitochondria; Molecular Sequence Data; Mutagenesis; Phosphotransferases (Alcohol Group Acceptor); Polymerase Chain Reaction; Rats; Saccharomyces cerevisiae | 1997 |
Heterologous HIS3 marker and GFP reporter modules for PCR-targeting in Saccharomyces cerevisiae.
Topics: Acyltransferases; Cell Cycle Proteins; Chromosome Mapping; Cloning, Molecular; Genes, Reporter; Genetic Complementation Test; Gentamicins; Green Fluorescent Proteins; Luminescent Proteins; Open Reading Frames; Peptide Elongation Factor 1; Peptide Elongation Factors; Plasmids; Polymerase Chain Reaction; Promoter Regions, Genetic; Recombination, Genetic; Saccharomyces; Saccharomyces cerevisiae; Saccharomyces cerevisiae Proteins; Schizosaccharomyces; Transcription Factors | 1997 |
Clonal variability in beta-globin mRNA content in an interleukin-3-dependent bone marrow cell line transfected with the erythropoietin receptor before and after stimulation with erythropoietin.
Topics: Animals; Anti-Bacterial Agents; Bone Marrow Cells; Butyrates; Butyric Acid; Cell Differentiation; Cell Line; Clone Cells; DNA-Binding Proteins; Erythropoiesis; Erythropoietin; Gene Expression Regulation, Developmental; Gentamicins; Globins; Interleukin-3; Mice; Milk Proteins; Receptors, Erythropoietin; RNA, Messenger; STAT5 Transcription Factor; Trans-Activators; Transfection | 1997 |
Evaluation of a cytokine combination including thrombopoietin for improved transduction of a retroviral gene into G-CSF-mobilized CD34+ human blood cells.
Topics: Anti-Bacterial Agents; Antigens, CD34; Cell Count; Cell Cycle; Child; Child, Preschool; Colony-Forming Units Assay; Cytokines; Drug Therapy, Combination; Evaluation Studies as Topic; Gene Transfer Techniques; Genes, Viral; Genetic Vectors; Gentamicins; Granulocyte Colony-Stimulating Factor; Hematopoietic Stem Cells; Humans; Interleukin-3; Interleukin-6; Retroviridae; Stem Cell Factor; Thrombopoietin; Transduction, Genetic | 1997 |
Improved EBV-based shuttle vector system: dicistronic mRNA couples the synthesis of the Epstein-Barr nuclear antigen-1 protein to neomycin resistance.
Topics: Binding Sites; Carcinoma; DNA Replication; Drug Resistance, Microbial; Encephalomyocarditis virus; Epstein-Barr Virus Nuclear Antigens; Genes; Genetic Vectors; Gentamicins; Humans; Kanamycin Kinase; Lung Neoplasms; Phosphotransferases (Alcohol Group Acceptor); Promoter Regions, Genetic; Replication Origin; Ribosomes; RNA, Messenger; Transcription, Genetic; Transfection; Tumor Cells, Cultured | 1997 |
n beta geo, a combined selection and reporter gene for retroviral and transgenic studies.
Topics: 3T3 Cells; Animals; Anti-Bacterial Agents; Antigens, Polyomavirus Transforming; beta-Galactosidase; Cell Nucleus; Escherichia coli; Gene Expression; Genes, Reporter; Genetic Markers; Genetic Vectors; Gentamicins; Histocytochemistry; Kanamycin Kinase; Mice; Recombinant Fusion Proteins; Retroviridae; Transgenes | 1997 |
pRIBOTEX expression vector: a pTEX derivative for a rapid selection of Trypanosoma cruzi transfectants.
Topics: Animals; Chloramphenicol O-Acetyltransferase; Cloning, Molecular; DNA, Protozoan; DNA, Ribosomal; Drug Resistance; Electroporation; Genes, Reporter; Genetic Vectors; Gentamicins; Transcription, Genetic; Transfection; Trypanosoma cruzi | 1997 |
Efficient gene transfer into primary and immortalized human fetal glial cells using adeno-associated virus vectors: establishment of a glial cell line with a functional CD4 receptor.
Topics: Anti-Bacterial Agents; Brain; CD4 Antigens; Cell Line; Cell Separation; Dependovirus; Flow Cytometry; Gene Transfer Techniques; Genetic Vectors; Gentamicins; Humans; Neuroglia; Transfection | 1997 |
Human factor IX transgenic sheep produced by transfer of nuclei from transfected fetal fibroblasts.
Topics: Animals; Animals, Genetically Modified; Cloning, Organism; Drug Resistance; Embryo Transfer; Factor IX; Female; Fetus; Fibroblasts; Gentamicins; Humans; Male; Milk; Neomycin; Nuclear Transfer Techniques; Oocytes; Recombinant Proteins; Sheep; Transfection; Transgenes | 1997 |
Introns enable the polyomavirus middle and small T antigens to stimulate the growth of primary rat embryo fibroblasts.
Topics: Animals; Anti-Bacterial Agents; Antigens, Polyomavirus Transforming; Cell Division; Cell Survival; Cell Transformation, Viral; Fibroblasts; Gene Expression Regulation; Genetic Vectors; Gentamicins; Introns; Mutagenesis, Site-Directed; Polymerase Chain Reaction; Rats; RNA Splicing; S Phase; Transcription, Genetic; Transfection | 1997 |
Generation of infectious retrovirus aerosol through medical laser irradiation.
Topics: 3T3 Cells; Aerosols; Animals; Anti-Bacterial Agents; Cell Line; DNA Primers; DNA, Viral; Drug Resistance, Microbial; Genetic Vectors; Gentamicins; Humans; Laser Therapy; Mice; Polymerase Chain Reaction; Retroviridae; RNA, Viral; Transfection; Virion | 1998 |
Osteoprogenitor cells as targets for ex vivo gene transfer.
Topics: Animals; Bone Marrow Cells; Bone Marrow Transplantation; Cell Lineage; Cells, Cultured; DNA Fragmentation; Gene Transfer Techniques; Genetic Vectors; Gentamicins; Male; Rats; Rats, Sprague-Dawley; Retroviridae; Stem Cell Transplantation; Stem Cells; Transduction, Genetic | 1998 |
Involvement of ceramide and JNK in CD40-mediated growth inhibition.
Topics: Amino Acid Substitution; Animals; Calcium-Calmodulin-Dependent Protein Kinases; CD40 Antigens; Cell Division; Cell Line; Ceramides; Drug Resistance; Fibroblasts; Gentamicins; Humans; JNK Mitogen-Activated Protein Kinases; Mitogen-Activated Protein Kinases; Mutagenesis, Site-Directed; Rats; Recombinant Proteins; Transfection; Tumor Cells, Cultured; Urinary Bladder Neoplasms | 1997 |
Comparison of retroviral-mediated gene transfer into cultured human CD34+ hematopoietic progenitor cells derived from peripheral blood, bone marrow, and fetal umbilical cord blood.
Topics: Animals; Blood Cells; Bone Marrow Cells; Breast Neoplasms; Cells, Cultured; Centrifugation; Colony-Forming Units Assay; Culture Media, Conditioned; Drug Resistance; Evaluation Studies as Topic; Female; Fetal Blood; Genes, Reporter; Genetic Vectors; Gentamicins; Hematopoietic Cell Growth Factors; Hematopoietic Stem Cells; Humans; Kanamycin Kinase; Mice; Polymerase Chain Reaction; Recombinant Fusion Proteins; Retroviridae; Transfection | 1997 |
A genetic system to detect mitotic recombination between repeated chromosomal sequences in Drosophila Schneider line 2 cells.
Topics: Animals; beta-Galactosidase; Cells, Cultured; Chromosomes; Drosophila melanogaster; Drug Resistance; Escherichia coli; Ethyl Methanesulfonate; Flow Cytometry; Genes, Reporter; Gentamicins; Methyl Methanesulfonate; Mitomycin; Mitosis; Mutagenicity Tests; Mutagens; Polymerase Chain Reaction; Recombination, Genetic; Repetitive Sequences, Nucleic Acid; Transgenes | 1997 |
Inhibition of expression of the lysine-rich 30 kDa surface antigen of Entamoeba dispar by the transcription of its antisense RNA.
Topics: Amebicides; Animals; Antigens, Protozoan; Antigens, Surface; Drug Resistance; Entamoeba; Gene Expression Regulation; Genes, Protozoan; Genetic Vectors; Gentamicins; RNA, Antisense; Transcription, Genetic; Transfection | 1997 |
Use of G418 resistance to select cells retrovirally transduced with the Neo(R) gene.
Topics: Bone Marrow Cells; Dose-Response Relationship, Drug; Drug Resistance; Fetal Blood; Gentamicins; Hematopoietic Stem Cells; Humans; Transduction, Genetic | 1998 |
Suitability of replacement markers for functional analysis studies in Saccharomyces cerevisiae.
Topics: Anti-Bacterial Agents; Base Sequence; Drug Resistance, Microbial; Genes, Fungal; Genetic Markers; Gentamicins; Histidine; Molecular Sequence Data; Saccharomyces cerevisiae | 1997 |
Co-insertional replication is responsible for tandem multimer formation during plasmid integration into the Dictyostelium genome.
Topics: Animals; Anti-Bacterial Agents; Dictyostelium; DNA Replication; Drug Resistance; Escherichia coli; Gene Dosage; Genetic Vectors; Gentamicins; Models, Genetic; Nucleosides; Plasmids; Transformation, Genetic | 1998 |
Expression of type X collagen and matrix calcification in three-dimensional cultures of immortalized temperature-sensitive chondrocytes derived from adult human articular cartilage.
Topics: Adult; Anti-Bacterial Agents; Calcification, Physiologic; Calcium; Cartilage, Articular; Cell Line; Cell Survival; Chondrocytes; Collagen; Drug Resistance, Microbial; Gentamicins; Glycosaminoglycans; Humans; Phenotype; Phosphorus; Retroviridae; RNA, Messenger; Simian virus 40; Temperature | 1998 |
Construction of PCR-ligated long flanking homology cassettes for use in the functional analysis of six unknown open reading frames from the left and right arms of Saccharomyces cerevisiae chromosome XV.
Topics: Anti-Bacterial Agents; Chromosomes, Fungal; Cloning, Molecular; DNA Repair; Drug Resistance, Microbial; Genetic Markers; Gentamicins; Mutagenesis, Insertional; Open Reading Frames; Phenotype; Plasmids; Polymerase Chain Reaction; Saccharomyces cerevisiae; Temperature | 1998 |
Stable DNA transfection of the primitive protozoan pathogen Giardia lamblia.
Topics: Animals; DNA Replication; Electroporation; Gene Dosage; Genes, Reporter; Genetic Vectors; Gentamicins; Giardia lamblia; Kanamycin Kinase; Luciferases; Nuclear Proteins; Plasmids; ran GTP-Binding Protein; Recombinant Proteins; Selection, Genetic; Transfection | 1998 |
A novel GFPneo vector designed for the isolation and analysis of enhancer elements in transfected mammalian cells.
Topics: Animals; Anti-Bacterial Agents; Cell Line; Cloning, Molecular; Desmin; Drug Resistance, Microbial; Enhancer Elements, Genetic; Flow Cytometry; Fluoresceins; Fluorescence; Gene Expression Regulation, Developmental; Genetic Vectors; Gentamicins; Green Fluorescent Proteins; Kanamycin Kinase; Luminescent Proteins; Muscle Fibers, Skeletal; Muscles; MyoD Protein; Polymerase Chain Reaction; Recombinant Proteins; Regulatory Sequences, Nucleic Acid; Transfection; Transgenes | 1998 |
Paromomycin and geneticin inhibit intracellular Cryptosporidium parvum without trafficking through the host cell cytoplasm: implications for drug delivery.
Topics: Animals; Anti-Bacterial Agents; Caco-2 Cells; Cattle; Cell Division; Cell Line; Cell Line, Transformed; Coccidiostats; Cryptosporidium parvum; Cytoplasm; Gentamicins; Humans; Kanamycin Kinase; Paromomycin; Protein Biosynthesis; Tritium | 1998 |
In utero gene therapy: transfer and long-term expression of the bacterial neo(r) gene in sheep after direct injection of retroviral vectors into preimmune fetuses.
Topics: Animals; Blotting, Southern; Enzyme-Linked Immunosorbent Assay; Erythroid Precursor Cells; Female; Fetal Diseases; Fluorescent Antibody Technique; Gene Transfer Techniques; Genetic Therapy; Genetic Vectors; Gentamicins; Hematopoietic Stem Cell Transplantation; In Situ Hybridization, Fluorescence; Injections, Intraperitoneal; Kanamycin Kinase; Polymerase Chain Reaction; Pregnancy; Retroviridae; Sequence Analysis, DNA; Sheep | 1998 |
Heterologous modules for efficient and versatile PCR-based gene targeting in Schizosaccharomyces pombe.
Topics: Drug Resistance, Microbial; Gene Deletion; Gene Expression; Gene Targeting; Genetic Markers; Gentamicins; Plasmids; Polymerase Chain Reaction; Recombination, Genetic; Schizosaccharomyces; Transformation, Genetic | 1998 |
Inhibition of erythroid differentiation and induction of megakaryocytic differentiation by thrombopoietin are regulated by two different mechanisms in TPO-dependent UT-7/c-mpl and TF-1/c-mpl cell lines.
Topics: Anti-Bacterial Agents; Cell Differentiation; Cell Division; Erythropoietin; Flow Cytometry; Fungal Proteins; Genetic Vectors; Gentamicins; Granulocyte-Macrophage Colony-Stimulating Factor; Humans; Megakaryocytes; Membrane Glycoproteins; Neoplasm Proteins; Platelet Glycoprotein GPIIb-IIIa Complex; Ploidies; Proto-Oncogene Proteins; Receptors, Cytokine; Receptors, Thrombopoietin; Thrombopoietin; Transcription Factors; Transfection; Tumor Cells, Cultured | 1998 |
Genetic analysis of adenovirus E1A: induction of genetic instability and altered cell morphologic and growth characteristics are segregatable functions.
Topics: 3T3 Cells; Adenoviridae; Adenovirus E1A Proteins; Animals; Anti-Bacterial Agents; Cell Division; Cell Size; Gene Expression; Gene Rearrangement; Genetic Complementation Test; Gentamicins; Kanamycin Kinase; Mice; Mutation; RNA Splicing; RNA, Messenger; Sequence Deletion; Transcriptional Activation; Transfection | 1998 |
Gene transfer into human umbilical cord blood-derived CD34+ cells by particle-mediated gene transfer.
Topics: Anti-Bacterial Agents; Antigens, CD34; Blotting, Southern; Cell Differentiation; Cell Separation; Cells, Cultured; Chloramphenicol O-Acetyltransferase; Coculture Techniques; Cytomegalovirus; Drug Resistance; Fetal Blood; Flow Cytometry; Gene Transfer Techniques; Genes, Reporter; Genetic Therapy; Genetic Vectors; Gentamicins; Gold Colloid; Hematopoietic Stem Cells; Humans; Neomycin; Plasmids; Polymerase Chain Reaction; Transfection | 1998 |
Exon shuffling by L1 retrotransposition.
Topics: Codon, Initiator; Exons; Expressed Sequence Tags; Gene Expression; Genome, Human; Gentamicins; HeLa Cells; Humans; Introns; Long Interspersed Nucleotide Elements; Poly A; Promoter Regions, Genetic; Recombination, Genetic; Transcription, Genetic | 1999 |
The chicken beta-globin 5'HS4 boundary element blocks enhancer-mediated suppression of silencing.
Topics: Animals; Chickens; DNA Nucleotidyltransferases; Drug Resistance; Enhancer Elements, Genetic; Gene Expression Regulation; Genes, Reporter; Gentamicins; Globins; Humans; Integrases; Mammals; Recombinases; Suppression, Genetic; Transgenes; Tumor Cells, Cultured | 1999 |
Suppression of Ras-mediated NIH3T3 transformation by p19ARF does not involve alterations of cell growth properties.
Topics: 3T3 Cells; Animals; Cell Transformation, Neoplastic; Cyclin-Dependent Kinase Inhibitor p16; Gentamicins; Humans; Mice; Proteins; ras Proteins; Suppression, Genetic; Tumor Suppressor Protein p14ARF | 1999 |
Expression of human growth hormone by the eukaryotic alga, Chlorella.
Topics: Amino Acid Sequence; Anti-Bacterial Agents; Blotting, Southern; Blotting, Western; Chlorella; Chloroquine; Cosmids; Electroporation; Gene Expression; Gentamicins; Hot Temperature; Human Growth Hormone; Introns; Molecular Sequence Data; Plasmids; Promoter Regions, Genetic; Protein Sorting Signals; Transfection | 1999 |
Changes during subclone development and ageing of human antibody-producing recombinant CHO cells.
Topics: Animals; Cellular Senescence; CHO Cells; Clone Cells; Cricetinae; Enzyme-Linked Immunosorbent Assay; Flow Cytometry; Fluorescent Antibody Technique; Gene Expression; Gentamicins; Humans; Hybridomas; Immunoglobulin G; Immunoglobulin Heavy Chains; Immunoglobulin Light Chains; In Situ Hybridization; Methotrexate; Recombinant Proteins; Tetrahydrofolate Dehydrogenase; Transfection | 1999 |
Replication of subgenomic hepatitis C virus RNAs in a hepatoma cell line.
Topics: Carcinoma, Hepatocellular; Cloning, Molecular; Drug Resistance; Genome, Viral; Gentamicins; Hepacivirus; Hepatitis C; Humans; Liver Neoplasms; Replicon; RNA, Viral; Transfection; Tumor Cells, Cultured; Viral Nonstructural Proteins; Virus Cultivation; Virus Replication | 1999 |
A set of independent selectable markers for transfection of the human malaria parasite Plasmodium falciparum.
Topics: Aminohydrolases; Animals; Antimalarials; Aspergillus; Cell Division; DNA Replication; DNA Transposable Elements; Drug Resistance; Gene Dosage; Genetic Markers; Gentamicins; Humans; Kanamycin Kinase; Nucleosides; Plasmodium falciparum; Polymorphism, Restriction Fragment Length; Promoter Regions, Genetic; Transfection | 1999 |
Faithful expression of a heterologous gene carried on an artificial macronuclear chromosome in Euplotes crassus.
Topics: 5' Untranslated Regions; Animals; Base Sequence; Cell Nucleus; Chromosomes; DNA-Binding Proteins; Euplotes; Gene Dosage; Gene Expression; Genes, Protozoan; Genes, Reporter; Gentamicins; Kanamycin Kinase; Liposomes; Molecular Sequence Data; Promoter Regions, Genetic; Recombinant Proteins; RNA, Messenger; TATA-Box Binding Protein; Telomere; Transcription Factors; Transcription, Genetic; Transformation, Genetic | 1999 |
Simultaneous Cre catalyzed recombination of two alleles to restore neomycin sensitivity and facilitate homozygous mutations.
Topics: Alleles; Animals; Anti-Bacterial Agents; Cell Line; Cytosine Deaminase; Drug Resistance; Gene Targeting; Genetic Markers; Gentamicins; GTP-Binding Protein alpha Subunit, Gi2; GTP-Binding Protein alpha Subunits, Gi-Go; GTP-Binding Proteins; Homozygote; Integrases; Loss of Heterozygosity; Mice; Mutation; Neomycin; Nucleoside Deaminases; Proto-Oncogene Proteins; Receptors, Cytoplasmic and Nuclear; Recombination, Genetic; Thymidine Kinase; Transcription Factors; Viral Proteins | 1999 |
Baby, don't stop!
Topics: Animals; Anti-Bacterial Agents; Codon, Nonsense; Genes, Bacterial; Genetic Engineering; Genetic Therapy; Gentamicins; Humans; Mice; Models, Genetic; Muscular Dystrophies; Mutation; Protein Biosynthesis; RNA, Ribosomal; RNA, Transfer, Amino Acyl | 1999 |
Expression of foreign proteins in Trypanosoma congolense.
Topics: Amino Acid Sequence; Animals; Cysteine Endopeptidases; Genetic Vectors; Gentamicins; Kanamycin Kinase; Membrane Glycoproteins; Molecular Sequence Data; Oligopeptides; Plasmids; Protein Biosynthesis; Proteins; Protozoan Proteins; Recombinant Proteins; Recombination, Genetic; Sequence Homology, Amino Acid; Trypanosoma congolense | 1999 |
Isolation and mutational analysis of a novel human cDNA, DEC1 (deleted in esophageal cancer 1), derived from the tumor suppressor locus in 9q32.
Topics: Amino Acid Sequence; Base Sequence; Blotting, Northern; Carcinoma, Small Cell; Chromosomes, Human, Pair 9; CpG Islands; DNA Methylation; DNA Mutational Analysis; DNA, Complementary; Esophageal Neoplasms; Female; Genes, Tumor Suppressor; Gentamicins; Humans; Lung Neoplasms; Male; Molecular Sequence Data; Polymorphism, Single-Stranded Conformational; Proteins; Sequence Analysis, DNA; Tissue Distribution; Tumor Cells, Cultured; Tumor Stem Cell Assay; Tumor Suppressor Proteins | 2000 |
Development of positive selectable markers for the fungal pathogen Cryptococcus neoformans.
Topics: Anti-Bacterial Agents; Biomarkers; Cryptococcus neoformans; Drug Resistance, Microbial; Gentamicins; Phleomycins; Serotyping | 2000 |
Trypanosoma cruzi: sensitivity to selective drugs in phylogenetically unrelated stocks.
Topics: Animals; Anti-Bacterial Agents; Gentamicins; Hygromycin B; Phylogeny; Trypanocidal Agents; Trypanosoma cruzi | 2000 |
T lymphocyte transduction with herpes simplex virus-thymidine kinase (HSV-tk) gene: comparison of four different infection protocols.
Topics: Cell Division; Cell Survival; Cells, Cultured; Centrifugation; Coculture Techniques; Culture Media, Conditioned; Drug Resistance, Microbial; Enzyme Induction; Fibronectins; Ganciclovir; Genes, Reporter; Genetic Vectors; Gentamicins; Immunophenotyping; Interleukin-2; Kanamycin Kinase; Lymphocyte Activation; Moloney murine leukemia virus; Phytohemagglutinins; Polymerase Chain Reaction; Reproducibility of Results; Safety; Simplexvirus; T-Lymphocytes; Thymidine Kinase; Transfection; Viral Proteins | 1999 |
Inactivation of six genes from chromosomes VII and XIV of Saccharomyces cerevisiae and basic phenotypic analysis of the mutant strains.
Topics: Anti-Bacterial Agents; Chromosomes, Fungal; Drug Resistance, Microbial; Fungal Proteins; Gene Deletion; Genes, Fungal; Gentamicins; Open Reading Frames; Phenotype; Plasmids; Polymerase Chain Reaction; Saccharomyces cerevisiae | 2000 |
Circular YAC vectors containing short mammalian origin sequences are maintained under selection as HeLa episomes.
Topics: Bromodeoxyuridine; Cell Division; Chromosomes, Artificial, Yeast; Chromosomes, Human, Pair 6; Cloning, Molecular; DNA Replication; DNA, Circular; Genes, Bacterial; Genes, Fungal; Genetic Vectors; Gentamicins; HeLa Cells; Humans; Plasmids; Replication Origin; Transfection; Transformation, Genetic | 2000 |
Influence of bcl-2 on cell death during the cultivation of a Chinese hamster ovary cell line expressing a chimeric antibody.
Topics: Ammonia; Animals; Anti-Bacterial Agents; Antibodies, Monoclonal; Antibody Formation; Apoptosis; Biotechnology; Blotting, Western; Cell Division; Cell Survival; CHO Cells; Cricetinae; Culture Media; Flow Cytometry; Gene Expression; Gentamicins; Proto-Oncogene Proteins c-bcl-2; Recombinant Fusion Proteins; Transfection | 2000 |
Shuttle vectors for genetic manipulations in Ustilago maydis.
Topics: Anti-Bacterial Agents; Carboxin; Cinnamates; Drug Resistance, Microbial; Fungicides, Industrial; Genetic Vectors; Gentamicins; Hygromycin B; Microbial Sensitivity Tests; Plasmids; Streptothricins; Ustilago | 2000 |
A spontaneous murine melanoma lung metastasis comprised of host x tumor hybrids.
Topics: Aminopterin; Animals; Anti-Bacterial Agents; Antigens, CD; Antineoplastic Combined Chemotherapy Protocols; Cell Movement; Chemotaxis; Flow Cytometry; Gentamicins; Hypoxanthine; Immunoblotting; Lung Neoplasms; Lysosomal Membrane Proteins; Melanoma; Membrane Glycoproteins; Mice; Mice, Inbred BALB C; Microscopy, Electron; Monophenol Monooxygenase; Neoplasm Transplantation; Polymorphism, Restriction Fragment Length; Sequence Analysis, DNA; Thymidine; Time Factors; Tumor Cells, Cultured | 2000 |
T-lymphocyte function after retroviral-mediated thymidine kinase gene transfer and G418 selection.
Topics: Anti-Bacterial Agents; Antigens, Differentiation, T-Lymphocyte; Cells, Cultured; DNA Primers; Dose-Response Relationship, Drug; Flow Cytometry; Ganciclovir; Gene Transfer Techniques; Gentamicins; Humans; Interleukin-2; Lymphocyte Activation; Polymerase Chain Reaction; Retroviridae; Simplexvirus; T-Lymphocytes; Thymidine Kinase; Time Factors | 2000 |
Comparison of two kinds of methods to determine the titer of recombinant retrovirus containing beta-globin gene based on G418 selection.
Topics: 3T3 Cells; Animals; Anti-Bacterial Agents; Genetic Engineering; Genetic Vectors; Gentamicins; Globins; Humans; Mice; Retroviridae; Virology | 2000 |
Aminoglycoside antibiotics mediate context-dependent suppression of termination codons in a mammalian translation system.
Topics: Animals; Anti-Bacterial Agents; Cinnamates; Codon, Terminator; Dose-Response Relationship, Drug; Escherichia coli; Genes, Reporter; Gentamicins; Humans; Hygromycin B; Kanamycin; Neomycin; Paromomycin; Plasmids; Protein Biosynthesis; Rabbits; Reticulocytes; Ribosomes; RNA, Messenger; Sisomicin; Streptomycin; Tobramycin | 2000 |
The "comparative growth assay": examining the interplay of anti-cancer agents with cells carrying single gene alterations.
Topics: Anti-Bacterial Agents; Antineoplastic Agents; ATP Binding Cassette Transporter, Subfamily G, Member 2; ATP-Binding Cassette Transporters; Breast Neoplasms; Cell Culture Techniques; Cell Division; Coculture Techniques; Colonic Neoplasms; Dose-Response Relationship, Drug; Dose-Response Relationship, Radiation; Drug Resistance, Neoplasm; Flow Cytometry; Fluorescent Dyes; Genetic Vectors; Gentamicins; Green Fluorescent Proteins; Humans; Luminescent Proteins; Methylnitronitrosoguanidine; Mitoxantrone; Mutation; Neoplasm Proteins; Time Factors; Transfection; Tumor Cells, Cultured | 1999 |
The ZF87/MAZ transcription factor functions as a growth suppressor in fibroblasts.
Topics: 3T3 Cells; Animals; Anti-Bacterial Agents; Antineoplastic Agents, Phytogenic; Camptothecin; Cell Division; Culture Media; Cyclin A; Cyclin E; DNA-Binding Proteins; G1 Phase; Gene Expression; Genes, myc; Genes, Reporter; Gentamicins; Immunoblotting; Mice; Retroviridae; S Phase; Transcription Factors; Transfection; Zinc Fingers | 2000 |
Cellular responses to sodium butyrate exhibit the dominance of one parental phenotype in somatic cell hybrids.
Topics: Butyrates; Cell Division; Choriocarcinoma; Chorionic Gonadotropin; Cycloheximide; DNA; DNA-Binding Proteins; Drug Resistance; Enhancer Elements, Genetic; Female; Gene Expression Regulation, Neoplastic; Genes, Dominant; Gentamicins; Glycoprotein Hormones, alpha Subunit; Humans; Hybrid Cells; Hygromycin B; Nuclear Proteins; Organ Specificity; Phenotype; Promoter Regions, Genetic; Response Elements; RNA, Messenger; Transfection; Trophoblasts; Tumor Cells, Cultured; Uterine Cervical Neoplasms | 2000 |
The use of the green fluorescent protein to monitor and improve transfection in Trypanosoma cruzi.
Topics: Animals; Anti-Bacterial Agents; Culture Media; Electroporation; Fluorescence; Gene Amplification; Gene Expression; Genetic Vectors; Gentamicins; Glycoproteins; Green Fluorescent Proteins; Luminescent Proteins; Neuraminidase; Plasmids; Transfection; Trypanosoma cruzi | 2000 |
Pharmacodynamic approach to study the gene transfer process employing non-viral vectors.
Topics: Animals; Anti-Bacterial Agents; DNA; Dose-Response Relationship, Drug; Fatty Acids, Monounsaturated; Gene Transfer Techniques; Genetic Vectors; Gentamicins; HeLa Cells; Humans; Mice; Plasmids; Quaternary Ammonium Compounds; Tumor Cells, Cultured | 2000 |
Controlling gene expression in yeast by inducible site-specific recombination.
Topics: Attachment Sites, Microbiological; Culture Media; DNA, Fungal; DNA, Recombinant; Enzyme Induction; Fungal Proteins; Gene Expression Regulation, Fungal; Gene Silencing; Genes, Fungal; Gentamicins; Humans; Integrases; Introns; Kinetics; Mutagenesis, Site-Directed; Receptors, Estrogen; Recombination, Genetic; Reproducibility of Results; RNA, Fungal; Saccharomyces cerevisiae; Sequence Deletion; Sequence Homology, Nucleic Acid; Silent Information Regulator Proteins, Saccharomyces cerevisiae; Templates, Genetic; Trans-Activators; Viral Proteins | 2000 |
Geneticin induces endogenous heme oxygenase-1 expression: implication in transfection studies.
Topics: Anti-Bacterial Agents; Cells, Cultured; DNA, Complementary; Dose-Response Relationship, Drug; Epithelial Cells; Gentamicins; Heme Oxygenase (Decyclizing); Heme Oxygenase-1; Humans; Kidney; Membrane Proteins; Oxidation-Reduction; RNA, Messenger; Time Factors; Transfection | 2001 |
Gene-transferred oligoclonal T cells predominantly persist in peripheral blood from an adenosine deaminase-deficient patient during gene therapy.
Topics: Adenosine Deaminase; Anti-Bacterial Agents; Cell Transplantation; DNA, Complementary; Genetic Therapy; Genetic Vectors; Gentamicins; Humans; Leukocytes, Mononuclear; Polymorphism, Single-Stranded Conformational; Reverse Transcriptase Polymerase Chain Reaction; Severe Combined Immunodeficiency; T-Lymphocytes | 2001 |
Constitutive expression of interleukin-8 by Mutatect cells markedly affects their tumor biology.
Topics: Animals; Cell Division; Drug Resistance; Enzyme-Linked Immunosorbent Assay; Fibrosarcoma; Genetic Vectors; Gentamicins; Humans; Injections, Subcutaneous; Interleukin-8; Mice; Mice, Inbred C57BL; Mutation; Neoplasm Transplantation; Neutrophils; Peroxidase; Polymerase Chain Reaction; Skin Neoplasms; Transfection; Tumor Cells, Cultured | 2001 |
Inhibition of gene expression in Entamoeba histolytica with antisense peptide nucleic acid oligomers.
Topics: Animals; Antisense Elements (Genetics); Biomarkers; Cell Division; Down-Regulation; Entamoeba histolytica; Gentamicins; Golgi Apparatus; Kanamycin Kinase; Membrane Proteins; Microscopy, Fluorescence; Neomycin; Peptide Nucleic Acids; Permeability; Transfection | 2001 |
Establishment of a novel human squamous cell carcinoma cell line from oral primary tumor by geneticin treatment.
Topics: Animals; Carcinoma, Squamous Cell; Cytological Techniques; Gentamicins; Gingival Neoplasms; Humans; Male; Mice; Neoplasm Transplantation; Transplantation, Heterologous; Tumor Cells, Cultured | 2000 |
In vitro and in vivo nucleotide exchange directed by chimeric RNA/DNA oligonucleotides in Saccharomyces cerevisae.
Topics: Base Sequence; Cell-Free System; DNA Repair; DNA Repair Enzymes; DNA-Binding Proteins; DNA, Fungal; Endonucleases; Fungal Proteins; Gentamicins; Kanamycin Kinase; Molecular Sequence Data; Mutation; Nucleotides; Oligonucleotides; Rad52 DNA Repair and Recombination Protein; RNA, Fungal; Saccharomyces cerevisiae; Saccharomyces cerevisiae Proteins; Selection, Genetic | 2001 |
Yeast vectors for integration at the HO locus.
Topics: Ampicillin Resistance; Deoxyribonucleases, Type II Site-Specific; DNA, Bacterial; DNA, Fungal; Drug Resistance, Microbial; Escherichia coli; Fungal Proteins; Genes, Fungal; Genetic Markers; Genetic Vectors; Gentamicins; Kanamycin Resistance; Molecular Sequence Data; Orotic Acid; Recombination, Genetic; Restriction Mapping; Saccharomyces cerevisiae; Saccharomyces cerevisiae Proteins; Tandem Repeat Sequences | 2001 |
Efficient gene transfer in mouse neural precursors with a bicistronic retroviral vector.
Topics: Alkaline Phosphatase; Animals; Astrocytes; Cell Differentiation; Cell Lineage; Corpus Striatum; Defective Viruses; Drug Resistance; Fibroblast Growth Factor 2; Fluorescent Antibody Technique; Gene Expression; Genes; Genes, Reporter; Genetic Vectors; Gentamicins; GPI-Linked Proteins; Isoenzymes; Kanamycin Kinase; Mice; Mice, Inbred C57BL; Moloney murine leukemia virus; Neurons; Oligodendroglia; Phenotype; Recombinant Fusion Proteins; Reticuloendotheliosis virus; Stem Cells; Transfection; Transgenes | 2001 |
MDR1 bicistronic vectors: analysis of selection stringency, amplified gene expression, and vector stability in cell lines.
Topics: 3T3 Cells; Animals; ATP Binding Cassette Transporter, Subfamily B, Member 1; Cell Line; Colchicine; Drug Resistance; Gene Amplification; Gene Expression; Gene Transfer Techniques; Genetic Therapy; Genetic Vectors; Gentamicins; Humans; K562 Cells; Mice; Nucleic Acid Amplification Techniques; Retroviridae; Selection, Genetic | 2001 |
Tetracycline-regulated gene expression mediated by a novel chimeric repressor that recruits histone deacetylases in mammalian cells.
Topics: Base Sequence; Cell Cycle Proteins; Cell Line; Gene Expression Regulation, Enzymologic; Genetic Vectors; Gentamicins; Glutathione Transferase; Green Fluorescent Proteins; Histone Deacetylases; Humans; Kinetics; Luciferases; Luminescent Proteins; Microscopy, Fluorescence; Molecular Sequence Data; Nuclear Proteins; Phosphoproteins; Promoter Regions, Genetic; Protein Binding; Protein Structure, Tertiary; Recombinant Fusion Proteins; Repressor Proteins; Sin3 Histone Deacetylase and Corepressor Complex; Tetracycline; Time Factors; Transfection; Tumor Cells, Cultured | 2001 |
Effect of retroviral transduction of canine microvascular endothelial cells on beta(1) integrin subunit expression and cell retention to PTFE grafts.
Topics: Animals; Anti-Bacterial Agents; Blood Vessel Prosthesis; Cell Adhesion; Cells, Cultured; Dogs; Endothelium, Vascular; Fibrin Tissue Adhesive; Gentamicins; Hemodynamics; Integrin beta1; Polytetrafluoroethylene; Retroviridae; Transduction, Genetic | 2001 |
Erythromycin slows aging of Saccharomyces cerevisiae.
Topics: Anti-Bacterial Agents; Dose-Response Relationship, Drug; Erythromycin; Gentamicins; Herbicides; Models, Biological; Paraquat; Saccharomyces cerevisiae | 2002 |
Retrovirus-mediated gene transfer in primary T lymphocytes impairs their anti-Epstein-Barr virus potential through both culture-dependent and selection process-dependent mechanisms.
Topics: Cell Culture Techniques; Cell Line, Transformed; Cytotoxicity Tests, Immunologic; Gene Transfer Techniques; Gentamicins; Herpesvirus 4, Human; HLA-A2 Antigen; Humans; Lymphocyte Activation; Retroviridae; T-Lymphocytes; T-Lymphocytes, Cytotoxic; T-Lymphocytes, Helper-Inducer; Transduction, Genetic | 2002 |
The radiation-induced bystander effect for clonogenic survival.
Topics: Alpha Particles; Animals; Bystander Effect; Cell Line; Cell Nucleus; Cell Survival; Clone Cells; Cricetinae; DNA; DNA Damage; Drug Resistance; Gentamicins; Neomycin; Transfection | 2002 |
Taurolidine: preclinical evaluation of a novel, highly selective, agent for bone marrow purging.
Topics: Animals; Antineoplastic Agents; Bone Marrow Purging; Bone Marrow Transplantation; Chimera; Drug Evaluation, Preclinical; Drug Resistance; Female; Gentamicins; HL-60 Cells; Humans; Mice; Mice, Inbred C57BL; Taurine; Thiadiazines; Transplantation, Autologous; Tumor Cells, Cultured; Tumor Stem Cell Assay | 2002 |
Translational errors as an early event in prion conversion.
Topics: Animals; Anti-Bacterial Agents; Frameshifting, Ribosomal; Gentamicins; Glutathione Peroxidase; Humans; Phenotype; Prions; Protein Biosynthesis; Protein Conformation; Protein Folding; Saccharomyces cerevisiae; Saccharomyces cerevisiae Proteins | 2001 |
Efficient biallelic mutagenesis with Cre/loxP-mediated inter-chromosomal recombination.
Topics: Animals; Chromosomes; Gentamicins; Homozygote; Integrases; Kanamycin Kinase; Mice; Mutagenesis; Receptors, Opioid, kappa; Recombination, Genetic; Stem Cells; Viral Proteins | 2002 |
Topological constraints governing the use of the chicken HS4 chromatin insulator in oncoretrovirus vectors.
Topics: 3' Untranslated Regions; Animals; Bone Marrow; Bone Marrow Transplantation; Cells, Cultured; Chickens; Chromatin; Enhancer Elements, Genetic; Gene Expression Regulation; Gene Silencing; Genetic Vectors; Gentamicins; Globins; Green Fluorescent Proteins; Humans; Kanamycin Kinase; Luminescent Proteins; Mice; Promoter Regions, Genetic; Regulatory Sequences, Nucleic Acid; Retroviridae; Terminal Repeat Sequences; Transduction, Genetic; Transgenes | 2002 |
Subgenomic replicon derived from a cell line infected with the hepatitis C virus.
Topics: Cell Line; Clone Cells; Genome, Viral; Gentamicins; Hepacivirus; Humans; Mutation; Replicon; RNA, Viral; Transfection; Viral Proteins; Virus Replication | 2002 |
A fibrous-bed bioreactor for continuous production of developmental endothelial locus-1 by osteosarcoma cells.
Topics: Anti-Bacterial Agents; Bioreactors; Blood Proteins; Calcium-Binding Proteins; Carrier Proteins; Cell Adhesion Molecules; Cell Count; Culture Media; Gentamicins; Glutamine; Humans; Indicator Dilution Techniques; Microscopy, Electron, Scanning; Models, Biological; Osteosarcoma; Polyesters; Tumor Cells, Cultured | 2002 |
Selection of transgenic Xenopus laevis using antibiotic resistance.
Topics: Animals; Animals, Genetically Modified; Biomarkers; Drug Resistance; Gentamicins; Xenopus laevis | 2002 |
Drug-induced translocation of nucleolar proteins fused to green fluorescent protein.
Topics: Dactinomycin; Gentamicins; Green Fluorescent Proteins; Humans; Luminescent Proteins; Microscopy; Nuclear Proteins; Protein Transport; Recombinant Fusion Proteins; Toyocamycin; Transfection | 2002 |
Deficiency in BRCA2 leads to increase in non-conservative homologous recombination.
Topics: Animals; BRCA2 Protein; CHO Cells; Cloning, Molecular; Cricetinae; DNA Damage; DNA Repair; DNA-Binding Proteins; Drug Resistance; Gene Conversion; Gentamicins; Mutation; Rad51 Recombinase; Recombination, Genetic | 2002 |
Rapid positive selection of stable integrants following transfection of Plasmodium falciparum.
Topics: Aminohydrolases; Animals; Anti-Bacterial Agents; Aspergillus; DNA Transposable Elements; Gene Targeting; Genetic Markers; Gentamicins; Kanamycin Kinase; Multienzyme Complexes; Nucleosides; Plasmodium falciparum; Recombinant Fusion Proteins; Recombination, Genetic; Selection, Genetic; Transcription, Genetic; Transfection | 2002 |
[Conditional targeting of p16(INK4a)exon 1a in mouse embryonic stem cells].
Topics: Animals; Anti-Bacterial Agents; Antiviral Agents; Base Sequence; Cell Division; Cell Line; Cyclin-Dependent Kinase Inhibitor p16; Drug Resistance; Embryo, Mammalian; Exons; Ganciclovir; Genetic Vectors; Gentamicins; Mice; Molecular Sequence Data; Recombination, Genetic; Stem Cells; Thymidine Kinase; Transfection | 2002 |
Aminoglycoside pretreatment partially restores the function of truncated V(2) vasopressin receptors found in patients with nephrogenic diabetes insipidus.
Topics: Amino Acid Sequence; Animals; Anti-Bacterial Agents; Base Sequence; Codon; COS Cells; Cricetinae; Diabetes Insipidus, Nephrogenic; Female; Fluorescent Antibody Technique; Gene Deletion; Gene Expression; Genetic Linkage; Gentamicins; Humans; Male; Molecular Sequence Data; Mutation; Receptors, Vasopressin; Sequence Alignment; Transfection; X Chromosome | 2002 |
Involvement of HMGB1 and HMGB2 proteins in exogenous DNA integration reaction into the genome of HeLa S3 cells.
Topics: Anti-Bacterial Agents; Drug Resistance; Gene Transfer Techniques; Gentamicins; HeLa Cells; HMGB1 Protein; HMGB2 Protein; Humans; Kanamycin Kinase; Plasmids; Recombination, Genetic | 2002 |
[Establishment of mouse ZF-12+/- embryonic stem cells].
Topics: Animals; Blotting, Southern; Carrier Proteins; Cell Division; Cell Survival; DNA; Electroporation; Embryo, Mammalian; Ganciclovir; Genetic Vectors; Genotype; Gentamicins; Mice; Mice, Inbred Strains; Molecular Sequence Data; Sequence Analysis, DNA; Transfection; Zinc Fingers | 2002 |
Crystal structure of geneticin bound to a bacterial 16S ribosomal RNA A site oligonucleotide.
Topics: Anti-Bacterial Agents; Binding Sites; Crystallography, X-Ray; Drug Design; Genes, Bacterial; Gentamicins; Humans; Models, Molecular; Molecular Structure; Nucleic Acid Conformation; Oligonucleotides; Protein Binding; Protein Conformation; RNA, Ribosomal, 16S | 2003 |
Retrofitting BACs with G418 resistance, luciferase, and oriP and EBNA-1 - new vectors for in vitro and in vivo delivery.
Topics: Animals; Biotechnology; Cell Line, Tumor; Chromosomes, Artificial, Bacterial; Electrophoresis, Agar Gel; Electroporation; Epstein-Barr Virus Nuclear Antigens; Escherichia coli; Gene Transfer Techniques; Genes, Reporter; Genetic Therapy; Genetic Vectors; Gentamicins; In Vitro Techniques; Luciferases; Melanoma, Experimental; Mice; Mice, Inbred BALB C; Models, Genetic; Muscles; Plasmids; Time Factors; Transfection | 2003 |
Transformation of Pythium aphanidermatum to geneticin resistance.
Topics: Electroporation; Genes, Reporter; Gentamicins; Pythium; Transfection | 2003 |
X-ray structure determination of Trypanosoma brucei ornithine decarboxylase bound to D-ornithine and to G418: insights into substrate binding and ODC conformational flexibility.
Topics: Allosteric Site; Animals; Anti-Bacterial Agents; Binding Sites; Crystallography, X-Ray; Dimerization; Electrons; Gentamicins; Inhibitory Concentration 50; Kinetics; Models, Molecular; Mutagenesis, Site-Directed; Ornithine; Ornithine Decarboxylase; Protein Binding; Protein Conformation; Protein Structure, Tertiary; Trypanosoma brucei brucei | 2003 |
Endogenous oxidoreductase expression is induced by aminoglycosides.
Topics: Aminoglycosides; Cell Line; Culture Media, Serum-Free; Dose-Response Relationship, Drug; Gentamicins; Glutaredoxins; Humans; Hygromycin B; Kanamycin; Neomycin; Oxidation-Reduction; Oxidoreductases; Pigment Epithelium of Eye; Protein Biosynthesis; Reactive Oxygen Species | 2003 |
High-efficiency plating method for Leishmania infantum.
Topics: Agar; Animals; Blood; Culture Media; Drug Resistance; Gentamicins; In Vitro Techniques; Leishmania infantum; Plasmids; Rabbits; Selection, Genetic; Transformation, Genetic; Trypanocidal Agents | 2003 |
The development of a transformation system for the dimorphic plant pathogen Holleya sinecauda based on Ashbya gossypii DNA elements.
Topics: DNA, Fungal; Electroporation; Genetic Markers; Genome, Fungal; Gentamicins; Kanamycin Resistance; Molecular Sequence Data; Open Reading Frames; Plants; Plasmids; Promoter Regions, Genetic; Recombination, Genetic; Saccharomycetales; Sex Characteristics; Transformation, Genetic | 2003 |
Modification of tumor cells with Fas (CD95) antigen gene and Fas ligand (CD95L) gene transfection by electroporation for immunotherapy of cancer.
Topics: Animals; Antibodies, Monoclonal; Cell Line, Tumor; Cell Survival; DNA, Complementary; Dose-Response Relationship, Drug; Drug Screening Assays, Antitumor; Electroporation; Fas Ligand Protein; fas Receptor; Female; Flow Cytometry; Gene Expression; Gentamicins; Immunotherapy; Inbreeding; Injections, Intradermal; Male; Membrane Glycoproteins; Mice; Mice, Inbred C3H; Neoplasms; Reverse Transcriptase Polymerase Chain Reaction; Transfection | 2003 |
Human alloreactive CTL interactions with gliomas and with those having upregulated HLA expression from exogenous IFN-gamma or IFN-gamma gene modification.
Topics: Anti-Inflammatory Agents; Apoptosis; Brain; Brain Neoplasms; Cells, Cultured; Cytokines; Dexamethasone; Edema; Enzyme-Linked Immunosorbent Assay; Flow Cytometry; Genetic Therapy; Gentamicins; Glioma; HLA Antigens; Humans; Immunosuppressive Agents; Interferon-gamma; Leukocytes, Mononuclear; Retroviridae; T-Lymphocytes, Cytotoxic; Time Factors; Up-Regulation | 2003 |
Effects of metalloporphyrins on heme oxygenase-1 transcription: correlative cell culture assays guide in vivo imaging.
Topics: Animals; Biological Assay; Cell Survival; Enhancer Elements, Genetic; Feasibility Studies; Gene Expression; Genes, Reporter; Gentamicins; Heme Oxygenase (Decyclizing); Heme Oxygenase-1; Image Processing, Computer-Assisted; Luminescent Measurements; Membrane Proteins; Metalloporphyrins; Mice; Mice, Transgenic; NIH 3T3 Cells; Promoter Regions, Genetic; Time Factors; Transcription, Genetic | 2003 |
Targeted gene replacement of a ferredoxin gene in Trichomonas vaginalis does not lead to metronidazole resistance.
Topics: Animals; Base Sequence; DNA Primers; Drug Resistance; Ferredoxins; Genetic Markers; Gentamicins; Kanamycin Kinase; Metronidazole; Models, Biological; Plasmids; Transcription, Genetic; Transformation, Bacterial; Trichomonas vaginalis | 2004 |
Arf tumor suppressor promoter monitors latent oncogenic signals in vivo.
Topics: Animals; Cell Transformation, Neoplastic; Cyclin-Dependent Kinase Inhibitor p16; DNA Primers; Electroporation; Genetic Vectors; Gentamicins; Green Fluorescent Proteins; Luminescent Proteins; Mice; Polymerase Chain Reaction; Recombinant Fusion Proteins; Stem Cells; Tumor Suppressor Protein p14ARF | 2003 |
The 492-bp RM07 DNA fragment from the halophilic Archaea confers promoter activity in all three domains of life.
Topics: Artificial Gene Fusion; Base Sequence; beta-Galactosidase; Chloramphenicol O-Acetyltransferase; DNA, Archaeal; Drug Resistance, Fungal; Escherichia coli; Genes, Reporter; Gentamicins; Halobacterium salinarum; Haloferax volcanii; Molecular Sequence Data; Mutagenesis, Site-Directed; Promoter Regions, Genetic; Reverse Transcriptase Polymerase Chain Reaction; RNA, Archaeal; Saccharomyces cerevisiae; Sequence Deletion; Transformation, Genetic | 2003 |
Limb bud progenitor cells induce differentiation of pluripotent embryonic stem cells into chondrogenic lineage.
Topics: Animals; Cell Differentiation; Cell Lineage; Cells, Cultured; Chondrocytes; Chondrogenesis; Coculture Techniques; Collagen Type II; DNA-Binding Proteins; Embryo, Mammalian; Female; Gene Expression Regulation; Gentamicins; High Mobility Group Proteins; Limb Buds; Mice; Mice, Inbred Strains; Octamer Transcription Factor-3; Pluripotent Stem Cells; Proteoglycans; SOX9 Transcription Factor; Transcription Factors | 2003 |
Genetic damage following introduction of DNA in Phycomyces.
Topics: DNA Replication; Drug Resistance, Bacterial; Genes, Fungal; Genome, Fungal; Gentamicins; Mitosis; Mutation; Phycomyces; Plasmids; Promoter Regions, Genetic; Spores, Fungal; Transformation, Genetic | 2004 |
New selectable markers and single crossover integration for the highly versatile Plasmodium knowlesi transfection system.
Topics: Aminohydrolases; Animals; Antiprotozoal Agents; Drug Resistance; Ganciclovir; Gene Expression; Genetic Markers; Gentamicins; Kanamycin Kinase; Nucleosides; Plasmids; Plasmodium knowlesi; Recombinant Proteins; Tetrahydrofolate Dehydrogenase; Thymidine Kinase; Transfection; Triazines | 2004 |
Aminoglycoside-mediated rescue of a disease-causing nonsense mutation in the V2 vasopressin receptor gene in vitro and in vivo.
Topics: Aminoglycosides; Animals; Cells, Cultured; Codon, Nonsense; COS Cells; Cricetinae; Diabetes Insipidus, Nephrogenic; Female; Gentamicins; Humans; Kidney Tubules, Collecting; Mice; Mice, Mutant Strains; Receptors, G-Protein-Coupled; Receptors, Vasopressin | 2004 |
Generation and partial characterization of a transformed cetacean cell line.
Topics: Acetylcysteine; Analysis of Variance; Animals; Antigens, Viral, Tumor; Cell Division; Cell Line, Transformed; Dolphins; Fluorescent Antibody Technique; Gentamicins; Glutamine; Glutathione; Keratins; Plasmids; Simian virus 40 | 2004 |
A multicopy vector system for genetic studies in Mucor circinelloides and other zygomycetes.
Topics: Amino Acid Sequence; Base Sequence; Drug Resistance, Fungal; Fungi; Genes, Fungal; Genetic Vectors; Gentamicins; Mucor; Mutation; Transformation, Genetic | 2004 |
Enhanced production of functional proteins from defective genes.
Topics: Aminoglycosides; Ataxia Telangiectasia Mutated Proteins; Base Sequence; Cell Cycle Proteins; Cell Line; Codon, Nonsense; Codon, Terminator; DNA-Binding Proteins; Gene Expression Regulation; Genes, Reporter; Gentamicins; Humans; Ofloxacin; Promoter Regions, Genetic; Protein Biosynthesis; Protein Serine-Threonine Kinases; Proteins; Reverse Transcriptase Polymerase Chain Reaction; RNA, Messenger; Sequence Homology, Nucleic Acid; Tumor Suppressor Proteins | 2004 |
Strain improvement for cephalosporin production by Acremonium chrysogenum using geneticin as a suitable transformation marker.
Topics: Acremonium; Anti-Bacterial Agents; Cephalosporins; Drug Resistance, Bacterial; Genes, Bacterial; Genetic Markers; Gentamicins; Transformation, Genetic | 2004 |
Cytochrome c release and endoplasmic reticulum stress are involved in caspase-dependent apoptosis induced by G418.
Topics: Animals; Anti-Bacterial Agents; Apoptosis; Caspases; Cytochromes c; Endoplasmic Reticulum; Gentamicins; Kidney; Microscopy, Electron; Microscopy, Fluorescence; Rats | 2004 |
Enhanced transgene expression in quiescent and activated human CD8+ T cells.
Topics: CD8-Positive T-Lymphocytes; Cell Line, Tumor; Drug Resistance; Gene Expression; Genetic Vectors; Gentamicins; Green Fluorescent Proteins; Humans; Interferon-beta; Lymphocyte Activation; Matrix Attachment Regions; Moloney murine leukemia virus; Promoter Regions, Genetic; Terminal Repeat Sequences; Transgenes | 2004 |
Directed evolution of ligand dependence: small-molecule-activated protein splicing.
Topics: Amebicides; Directed Molecular Evolution; Estrogen Antagonists; Gentamicins; Ligands; Models, Molecular; Protein Splicing; Recombinant Fusion Proteins; Saccharomyces cerevisiae; Saccharomyces cerevisiae Proteins; Tamoxifen | 2004 |
Use and comparison of different internal ribosomal entry sites (IRES) in tricistronic retroviral vectors.
Topics: Adenocarcinoma; Animals; Antigens, CD; B7-1 Antigen; Bleomycin; CD27 Ligand; Cell Line, Tumor; Drug Resistance; Gene Expression Regulation; Gene Transfer Techniques; Genes, Viral; Genetic Vectors; Gentamicins; Humans; Kidney; Mammary Neoplasms, Animal; Melanoma; Melanoma, Experimental; Membrane Proteins; Mice; NIH 3T3 Cells; Retroviridae; Ribosomes; RNA, Messenger; Skin Neoplasms; Transduction, Genetic; Transgenes; Viral Structural Proteins | 2004 |
Integration and long-term expression in xenografted human glioblastoma cells using a plasmid-based transposon system.
Topics: Animals; Base Sequence; Cell Line, Tumor; DNA Transposable Elements; Female; Gene Expression; Genetic Therapy; Genetic Vectors; Gentamicins; Glioblastoma; Green Fluorescent Proteins; Humans; Luciferases; Mice; Mice, Nude; Molecular Sequence Data; Plasmids; Polyethyleneimine; Transposases; Xenograft Model Antitumor Assays | 2004 |
Correction of ATM gene function by aminoglycoside-induced read-through of premature termination codons.
Topics: Aminoglycosides; Ataxia Telangiectasia; Ataxia Telangiectasia Mutated Proteins; Base Sequence; Cell Cycle Proteins; Cell Line; Codon, Nonsense; DNA; DNA-Binding Proteins; Gentamicins; Humans; In Vitro Techniques; Paromomycin; Point Mutation; Protein Serine-Threonine Kinases; Radiation Tolerance; Tobramycin; Tumor Suppressor Proteins | 2004 |
Isolation and culture of amelanotic melanocytes from human hair follicles.
Topics: Cells, Cultured; Gentamicins; Hair Follicle; Humans; Immunohistochemistry; Melanocytes; Tetradecanoylphorbol Acetate | 2004 |
Functional genetic screen for genes involved in senescence: role of Tid1, a homologue of the Drosophila tumor suppressor l(2)tid, in senescence and cell survival.
Topics: Animals; Anti-Bacterial Agents; Cell Survival; Cells, Cultured; Cellular Senescence; Electrophoretic Mobility Shift Assay; Embryo, Mammalian; Fibroblasts; Genes, Reporter; Gentamicins; Heat-Shock Proteins; HSP40 Heat-Shock Proteins; Luciferases; Mice; Mice, Inbred C57BL; Models, Biological; Molecular Chaperones; NF-kappa B; Proteins; Rats; Rats, Sprague-Dawley; Retroviridae; RNA; RNA Interference; Tumor Necrosis Factor-alpha; Tumor Suppressor Proteins | 2004 |
[Liposome-mediated gene transfer into bone marrow cells and its in vitro expansion].
Topics: Bone Marrow Cells; Cell Proliferation; Galactosides; Gene Transfer Techniques; Gentamicins; Humans; Indoles; Liposomes; Staining and Labeling | 1997 |
Defining the basis for the specificity of aminoglycoside-rRNA recognition: a comparative study of drug binding to the A sites of Escherichia coli and human rRNA.
Topics: Aminoglycosides; Base Sequence; Binding Sites; Calorimetry; Escherichia coli; Gentamicins; Humans; Hydrogen-Ion Concentration; Models, Molecular; Nucleic Acid Conformation; Paromomycin; Protein Structure, Tertiary; Protons; RNA, Ribosomal; Spectrometry, Fluorescence; Static Electricity; Substrate Specificity; Temperature; Thermodynamics; Time Factors; Titrimetry | 2005 |
Selection of high-producing CHO cells using NPT selection marker with reduced enzyme activity.
Topics: Amino Acid Motifs; Amino Acid Sequence; Animals; Antibodies, Monoclonal; Cell Survival; Chemokine CCL2; CHO Cells; Clone Cells; Cricetinae; Cricetulus; Gene Expression; Genetic Markers; Gentamicins; Kanamycin Kinase; Molecular Sequence Data; Point Mutation; Protein Structure, Tertiary; Recombinant Proteins; Selection, Genetic; Transfection | 2005 |
Visualization of endogenous p53-mediated transcription in vivo using sodium iodide symporter.
Topics: Animals; Biological Transport; Blotting, Western; Cell Line, Tumor; DNA, Complementary; Dose-Response Relationship, Drug; Doxorubicin; Gene Expression Regulation, Neoplastic; Genes, Reporter; Genetic Vectors; Gentamicins; Humans; Male; Mice; Mice, Inbred BALB C; Mice, Nude; Neoplasm Transplantation; Perchlorates; Plasmids; Potassium Compounds; Radionuclide Imaging; Reverse Transcriptase Polymerase Chain Reaction; Symporters; Time Factors; Transcription, Genetic; Transcriptional Activation; Transfection; Tumor Suppressor Protein p53; Up-Regulation | 2005 |
Construction of two new vectors for transformation of laboratory, natural and industrial Saccharomyces cerevisiae strains to trifluoroleucine and G418 resistance.
Topics: Base Sequence; DNA, Fungal; Drug Resistance, Fungal; Genes, Fungal; Genetic Vectors; Gentamicins; Leucine; Mutagenesis, Site-Directed; Plasmids; Saccharomyces cerevisiae; Species Specificity; Transformation, Genetic | 2004 |
Efficient replication of a full-length hepatitis C virus genome, strain O, in cell culture, and development of a luciferase reporter system.
Topics: Amino Acid Substitution; Cell Culture Techniques; Cell Line, Tumor; Cell Proliferation; Genes, Reporter; Genome, Viral; Gentamicins; Hepacivirus; Humans; Luciferases; Lysine; Molecular Sequence Data; Mutation; RNA, Viral; Virus Replication | 2005 |
Simple and straightforward construction of a mouse gene targeting vector using in vitro transposition reactions.
Topics: Alleles; Animals; Base Sequence; Bleomycin; DNA Transposable Elements; Escherichia coli; Gene Targeting; Genetic Vectors; Gentamicins; Kanamycin Resistance; Mice; Molecular Sequence Data; Plasmids; Promoter Regions, Genetic; Recombination, Genetic; Vesicular Transport Proteins | 2005 |
Agrobacterium tumefaciens-mediated transformation of leek (Allium porrum) and garlic (Allium sativum).
Topics: Agrobacterium tumefaciens; Coccidiostats; Garlic; Gene Expression Regulation, Plant; Genes, Reporter; Genetic Markers; Genetic Vectors; Genome, Plant; Gentamicins; Onions; Plants, Genetically Modified; Seeds; Transformation, Genetic; Transgenes | 2005 |
Biological activity of antibiotic G-418, a new micromonospora-produced aminoglycoside with activity against protozoa and helminths.
Topics: Animals; Anti-Bacterial Agents; Bacteria; Eukaryota; Gentamicins; Helminths; Male; Mice | 1974 |
Antibiotic G-418, a new Micromonospora-produced aminoglycoside with activity against protozoa and helminths: fermentation, isolation, and preliminary characterization.
Topics: Aminoglycosides; Animals; Anti-Bacterial Agents; Eukaryota; Fermentation; Gentamicins; Helminths; Micromonospora | 1974 |
New drug-resistant cassettes for gene disruption and epitope tagging in Schizosaccharomyces pombe.
Topics: Drug Resistance, Bacterial; Epitopes; Gene Targeting; Genes, Bacterial; Genes, Fungal; Genetic Markers; Genetic Vectors; Gentamicins; Hygromycin B; Luminescent Proteins; Plasmids; Polymerase Chain Reaction; Red Fluorescent Protein; Schizosaccharomyces; Streptothricins; Transformation, Genetic | 2005 |
PCR-based gene disruption and recombinatory marker excision to produce modified industrial Saccharomyces cerevisiae without added sequences.
Topics: Base Sequence; Deoxyribonucleases, Type II Site-Specific; Gene Deletion; Genetic Markers; Gentamicins; Industrial Microbiology; Kanamycin Resistance; Molecular Sequence Data; Polymerase Chain Reaction; Recombination, Genetic; Repetitive Sequences, Nucleic Acid; Saccharomyces cerevisiae; Saccharomyces cerevisiae Proteins; Wine | 2005 |
A novel yeast genomic DNA library on a geneticin-resistance vector.
Topics: Aldose-Ketose Isomerases; Antifungal Agents; DNA, Fungal; Drug Resistance, Fungal; Gene Expression Regulation, Fungal; Genetic Complementation Test; Genetic Vectors; Genomic Library; Gentamicins; Saccharomyces cerevisiae; Saccharomyces cerevisiae Proteins; Transformation, Genetic | 2005 |
Transfection of Trypanosoma cruzi with host CD40 ligand results in improved control of parasite infection.
Topics: Animals; Antibodies, Protozoan; CD40 Ligand; Cell Proliferation; Chagas Disease; Genetic Vectors; Gentamicins; Interferon-gamma; Macrophages, Peritoneal; Mice; Mice, Inbred Strains; Parasitemia; Protozoan Vaccines; Spleen; Transfection; Trypanosoma cruzi | 2005 |
Role of deadenylation and AUF1 binding in the pH-responsive stabilization of glutaminase mRNA.
Topics: Adenosine Monophosphate; Animals; beta-Crystallins; Cell Line; Cytosol; Gentamicins; Glutaminase; Heterogeneous Nuclear Ribonucleoprotein D0; Heterogeneous-Nuclear Ribonucleoprotein D; Hydrogen-Ion Concentration; Hygromycin B; Kidney Cortex; Male; Mutagenesis; Rats; Rats, Sprague-Dawley; Ribonuclease H; RNA, Messenger | 2006 |
Self renewal, expansion, and transfection of rat spermatogonial stem cells in culture.
Topics: Animals; Cell Culture Techniques; Cell Proliferation; Flow Cytometry; Gentamicins; Green Fluorescent Proteins; Kanamycin Kinase; Male; Plasmids; Rats; Spermatogonia; Stem Cells; Testis; Transfection | 2005 |
Exogenous gene expression and growth regulation of hematopoietic cells via a novel human artificial chromosome.
Topics: Animals; Chromosomes, Artificial, Human; Chromosomes, Human, Pair 21; Cord Blood Stem Cell Transplantation; DNA Primers; Flow Cytometry; Gene Expression; Genetic Therapy; Genetic Vectors; Gentamicins; Green Fluorescent Proteins; Hematopoietic Stem Cells; Humans; Mice | 2006 |
A site-directed integration system for the nonuniversal CUG(Ser) codon usage species Pichia farinosa by electroporation.
Topics: Amino Acid Sequence; Base Sequence; Bleomycin; Codon; Electroporation; Genetic Engineering; Genetic Vectors; Gentamicins; Molecular Sequence Data; Pichia; Transformation, Genetic | 2006 |
System of centromeric, episomal, and integrative vectors based on drug resistance markers for Saccharomyces cerevisiae.
Topics: Anti-Bacterial Agents; Centromere; Drug Resistance, Fungal; Genetic Engineering; Genetic Markers; Genetic Vectors; Gentamicins; Hygromycin B; Plasmids; Saccharomyces cerevisiae; Streptothricins; Transformation, Genetic | 2006 |
Beta-cell differentiation from nonendocrine epithelial cells of the adult human pancreas.
Topics: Adult; Animals; Cell Differentiation; Cell Fusion; Cell Transplantation; Cell- and Tissue-Based Therapy; Cells, Cultured; DNA Replication; Epithelial Cells; Fetus; Gentamicins; Humans; Insulin; Islets of Langerhans; Mesoderm; Mice; Mice, SCID; Middle Aged | 2006 |
Construction of pcDNA3.1-based vectors with blasticidin and puromycin resistance markers.
Topics: Animals; Biomarkers; CHO Cells; Cloning, Molecular; Cricetinae; DNA, Complementary; Genetic Vectors; Gentamicins; Nucleosides; Puromycin | 2006 |
Intracellular readthrough of nonsense mutations by aminoglycosides in coagulation factor VII.
Topics: Adolescent; Animals; Anti-Bacterial Agents; Blood Coagulation; Cell Line; Child, Preschool; Codon, Nonsense; Cricetinae; Dose-Response Relationship, Drug; Factor VII; Factor VII Deficiency; Gene Expression Regulation; Gentamicins; Green Fluorescent Proteins; Humans; Male; Recombinant Fusion Proteins; Transfection | 2006 |
Expression and distribution of HSP27 in response to G418 in different human breast cancer cell lines.
Topics: Blotting, Western; Breast Neoplasms; Cell Line, Tumor; Cell Nucleus; Female; Gentamicins; Green Fluorescent Proteins; Heat-Shock Proteins; HSP27 Heat-Shock Proteins; Humans; Molecular Chaperones; Neoplasm Proteins; Protein Transport; Transfection | 2006 |
Exogenous control of mammalian gene expression via modulation of translational termination.
Topics: Acetanilides; Aminobenzoates; Aminoglycosides; Animals; Cell Line; Cells, Cultured; Codon, Terminator; Gene Expression Regulation; Genetic Engineering; Genetic Vectors; Gentamicins; Luciferases; Mice; Peptide Chain Termination, Translational; Transgenes | 2006 |
DNA damage binding protein component DDB1 participates in nucleotide excision repair through DDB2 DNA-binding and cullin 4A ubiquitin ligase activity.
Topics: Cullin Proteins; DNA Damage; DNA Repair; DNA-Binding Proteins; Fibroblasts; Gentamicins; HeLa Cells; Humans; Recombinant Proteins; Transfection; Ubiquitin-Protein Ligases; Ultraviolet Rays | 2006 |
Bystander killing of breast cancer MCF-7 cells by MDA-MB-231 cells exposed to 5-fluorouracil is mediated via Fas.
Topics: Antimetabolites, Antineoplastic; Apoptosis; Breast Neoplasms; Bystander Effect; Caspase 8; Cell Line, Tumor; Clone Cells; Coculture Techniques; Fas Ligand Protein; fas Receptor; Female; Fluorouracil; Gentamicins; Green Fluorescent Proteins; Humans; Luminescent Measurements; Transfection | 2007 |
[Establishment of a transgenic cell model for preliminary screening of chemopreventive agents].
Topics: Antineoplastic Agents; Base Sequence; Dose-Response Relationship, Drug; Drug Screening Assays, Antitumor; Enhancer Elements, Genetic; Gene Expression; Gentamicins; Green Fluorescent Proteins; Hep G2 Cells; Humans; Lentinan; Microscopy, Fluorescence; Molecular Sequence Data; Oligonucleotides; Proline; Recombinant Fusion Proteins; Thiocarbamates; Transfection | 2007 |
Tools and methods for genetic analysis of Saccharomyces castellii.
Topics: Acetates; Antifungal Agents; Computational Biology; DNA, Fungal; Drug Resistance, Fungal; Gene Dosage; Gene Expression Regulation, Fungal; Genetic Vectors; Gentamicins; Mycology; Plasmids; Saccharomyces; Saccharomyces cerevisiae; Spores, Fungal; Transformation, Genetic | 2007 |
G418, phleomycin and hygromycin selection of recombinant Trypanosoma brucei parasites refractory to long-term in vitro culture.
Topics: Adaptation, Biological; Animals; Antiprotozoal Agents; Cinnamates; Female; Gentamicins; Hygromycin B; Male; Mice; Phleomycins; Selection, Genetic; Trypanosoma brucei brucei | 2007 |
Transgenic sequences are frequently lost in Phytophthora parasitica transformants without reversion of the transgene-induced silenced state.
Topics: Algal Proteins; Drug Resistance; Gene Silencing; Gentamicins; Hygromycin B; Pesticides; Phytophthora; Plants, Genetically Modified; Protoplasts; Spores; Transformation, Genetic | 2007 |
Restoration of W1282X CFTR activity by enhanced expression.
Topics: Amino Acid Substitution; Butyrates; Cell Line; Codon, Nonsense; Cystic Fibrosis; Cystic Fibrosis Transmembrane Conductance Regulator; Gene Expression; Gentamicins; HeLa Cells; Humans; Ion Transport; Reverse Transcriptase Polymerase Chain Reaction; RNA, Messenger; Suppression, Genetic; Transduction, Genetic | 2007 |
[Quick testing-technology of transgenic rice with npt- screen marker gene].
Topics: Bacterial Proteins; Biomarkers; Clinical Laboratory Techniques; Gene Expression Regulation, Plant; Gentamicins; Kanamycin; Membrane Transport Proteins; Oryza; Plant Leaves; Plants, Genetically Modified; Seedlings; Seeds | 2007 |
Cancerogenic effect of different fragments of the hepatitis C virus core protein.
Topics: Animals; Blotting, Western; Cell Line; Drug Resistance, Neoplasm; Female; Gentamicins; Hepatitis C; Hepatocytes; Humans; Liver Neoplasms; Mice; Mice, Nude; Neoplasms; Peptide Fragments; Protein Transport; Recombinant Fusion Proteins; Subcellular Fractions; Viral Core Proteins | 2007 |
A molecular toolbox for manipulating Eremothecium coryli.
Topics: Antifungal Agents; Artificial Gene Fusion; beta-Galactosidase; Drug Resistance, Fungal; Genes, Reporter; Genetic Vectors; Gentamicins; Green Fluorescent Proteins; Microbiological Techniques; Molecular Biology; Plasmids; Promoter Regions, Genetic; Saccharomyces cerevisiae; Saccharomycetales; Streptothricins; Transformation, Genetic | 2007 |
A mouse model for nonsense mutation bypass therapy shows a dramatic multiday response to geneticin.
Topics: Amebicides; Aminoglycosides; Animals; Antineoplastic Agents; Codon, Nonsense; Codon, Terminator; Disease Models, Animal; Gentamicins; Hemophilia A; Humans; Liver; Mice; Mice, Inbred C57BL; Models, Genetic; Protein Biosynthesis | 2007 |
Identification of human kinases involved in hepatitis C virus replication by small interference RNA library screening.
Topics: Cell Line, Tumor; CSK Tyrosine-Protein Kinase; Gentamicins; Hepacivirus; Humans; Immunoblotting; Intracellular Signaling Peptides and Proteins; Janus Kinase 1; Luciferases, Firefly; Phosphorylation; Phosphotransferases; Protein Kinase Inhibitors; Protein Serine-Threonine Kinases; Protein-Tyrosine Kinases; Proto-Oncogene Proteins; Replicon; Reverse Transcriptase Polymerase Chain Reaction; RNA Interference; RNA, Small Interfering; RNA, Viral; src-Family Kinases; Virus Replication | 2008 |
Functional analysis of the interplay between translation termination, selenocysteine codon context, and selenocysteine insertion sequence-binding protein 2.
Topics: 3' Untranslated Regions; Animals; Cell-Free System; Coccidiostats; Codon, Terminator; Gentamicins; Luciferases; Peptide Chain Termination, Translational; Peptide Elongation Factors; Peptide Termination Factors; Rabbits; Rats; RNA-Binding Proteins; RNA, Transfer, Amino Acyl; Selenocysteine; Selenoprotein P | 2007 |
Programmed cell death in Entamoeba histolytica induced by the aminoglycoside G418.
Topics: Amebicides; Animals; Apoptosis; Calcium; DNA Fragmentation; Entamoeba histolytica; Gentamicins; Hydrogen-Ion Concentration; Oxidative Stress; Potassium; Reactive Oxygen Species; Trophozoites | 2007 |
Genetic transformation of the dermatophyte, Trichophyton mentagrophytes, based on the use of G418 resistance as a dominant selectable marker.
Topics: Amebicides; Culture Media; Cyclophilins; Drug Resistance, Fungal; Genetic Markers; Gentamicins; Green Fluorescent Proteins; Plasmids; Polyethylene Glycols; Protoplasts; Recombinant Fusion Proteins; Transformation, Genetic; Trichophyton | 2008 |
Human somatic cell gene targeting.
Topics: Alleles; Cell Line; Drug Resistance; Gene Targeting; Genetic Vectors; Gentamicins; Heterozygote; Humans; Integrases; Kanamycin Kinase; Transfection | 2003 |
Direct selection of Pichia pastoris expression strains using new G418 resistance vectors.
Topics: Cloning, Molecular; Gene Dosage; Genetic Techniques; Genetic Vectors; Gentamicins; Kanamycin; Pichia; Polymerase Chain Reaction; Promoter Regions, Genetic; RNA, Fungal; Selection, Genetic; Transformation, Genetic | 2008 |
A simple method for isolating disomic strains of Saccharomyces cerevisiae.
Topics: Chromosomes, Fungal; Diploidy; Gentamicins; Haploidy; Mutagenesis, Insertional; Open Reading Frames; Polymerase Chain Reaction; Recombination, Genetic; Saccharomyces cerevisiae; Saccharomyces cerevisiae Proteins; Spores, Fungal; Transformation, Genetic | 2008 |
Bursera fagaroides, effect of an ethanolic extract on ornithine decarboxylase (ODC) activity in vitro and on the growth of Entamoeba histolytica.
Topics: Amebicides; Animals; Antiprotozoal Agents; Bursera; Entamoeba histolytica; Entamoebiasis; Gentamicins; Metronidazole; Ornithine Decarboxylase; Ornithine Decarboxylase Inhibitors; Parasitic Sensitivity Tests; Plant Extracts | 2008 |
Antiviral activity of geneticin against bovine viral diarrhoea virus.
Topics: Animals; Antiviral Agents; Bovine Virus Diarrhea-Mucosal Disease; Cattle; Cell Survival; Cells, Cultured; Diarrhea Virus 1, Bovine Viral; Gentamicins; Interferons; Kanamycin; Kidney; Neutralization Tests; Peptide Hydrolases; Reverse Transcriptase Polymerase Chain Reaction; RNA Helicases; RNA, Viral; Structure-Activity Relationship; Viral Nonstructural Proteins; Virus Replication | 2008 |
G418-mediated ribosomal read-through of a nonsense mutation causing autosomal recessive proximal renal tubular acidosis.
Topics: Acidosis, Renal Tubular; Amebicides; Cell Line; Codon, Nonsense; Gene Expression; Gentamicins; Humans; Immunoblotting; Immunohistochemistry; RNA, Messenger; Sodium-Bicarbonate Symporters | 2008 |
Monitoring miRNA-mediated silencing in Drosophila melanogaster S2-cells.
Topics: Animals; Cell Line; Culture Media; Drosophila melanogaster; Drosophila Proteins; Gene Knockdown Techniques; Gene Silencing; Genes, Reporter; Gentamicins; MicroRNAs; RNA Interference; RNA, Double-Stranded; Transfection | 2008 |
Systemic aminoglycoside treatment in rodent models of retinitis pigmentosa.
Topics: Aminoglycosides; Animals; Carrier Proteins; cis-trans-Isomerases; Codon, Nonsense; Disease Models, Animal; Dose-Response Relationship, Drug; Drug Evaluation, Preclinical; Electroretinography; Eye Proteins; Fluorescent Dyes; Gentamicins; Mice; Mice, Mutant Strains; Rats; Rats, Transgenic; Retina; Retinitis Pigmentosa; Rhodopsin; Xanthenes | 2008 |
Selection by drug resistance proteins located in the mitochondria of mammalian cells.
Topics: Cell Proliferation; Cinnamates; Drug Resistance; Gentamicins; HeLa Cells; Humans; Hygromycin B; Kanamycin Kinase; Mitochondria; Phosphotransferases (Alcohol Group Acceptor); Recombinant Fusion Proteins | 2008 |
Ligand selectivity of D2 dopamine receptors is modulated by changes in local dynamics produced by sodium binding.
Topics: Amebicides; Colforsin; Cyclic AMP; Gentamicins; Humans; Kidney; Kinetics; Ligands; Models, Molecular; Mutagenesis, Site-Directed; Protein Conformation; Quinpirole; Radioligand Assay; Receptors, Dopamine D2; Recombinant Proteins; Sodium; Transfection | 2009 |
Post-translational modifications guard yeast from misaspartylation.
Topics: Amino Acid Sequence; Aminoacylation; Aspartate-tRNA Ligase; Aspartic Acid; Biotin; Gene Expression; Gentamicins; Glycosylation; Lysine; Mass Spectrometry; Methylation; Models, Molecular; Molecular Sequence Data; Protein Conformation; Protein Processing, Post-Translational; Proteome; Saccharomyces cerevisiae | 2008 |
Suppression of the tumorigenicity of B-16V melanoma cells via lysozyme gene.
Topics: Animals; Cloning, Molecular; Gene Expression Regulation, Neoplastic; Genetic Therapy; Genetic Vectors; Gentamicins; Melanoma; Melanoma, Experimental; Mice; Models, Statistical; Muramidase; Odds Ratio; Plasmids; Time Factors; Treatment Outcome | 2008 |
Production of transgenic pigs harboring the human erythropoietin (hEPO) gene using somatic cell nuclear transfer.
Topics: Animals; Animals, Genetically Modified; Animals, Newborn; DNA; Erythropoietin; Female; Gene Dosage; Genetic Vectors; Gentamicins; Humans; In Situ Hybridization, Fluorescence; Karyotyping; Nuclear Transfer Techniques; Polymerase Chain Reaction; Pregnancy; Swine; Transfection | 2009 |
[Construction of universal vector for gene targeting and analysis of its function].
Topics: Animals; Animals, Genetically Modified; Cloning, Molecular; Ganciclovir; Gene Targeting; Genetic Vectors; Gentamicins; Goats; Integrases; Neomycin; Phosphotransferases | 2008 |
Translational readthrough by the aminoglycoside geneticin (G418) modulates SMN stability in vitro and improves motor function in SMA mice in vivo.
Topics: Aminoglycosides; Animals; Base Sequence; Cell Line; Codon, Terminator; Exons; Fibroblasts; Gentamicins; Humans; Mice; Molecular Sequence Data; Motor Activity; Muscular Atrophy, Spinal; Protein Biosynthesis; Protein Stability; Survival of Motor Neuron 1 Protein | 2009 |
Agrobacterium-mediated transformation of embryogenic tissues of hybrid firs (Abies spp.) and regeneration of transgenic emblings.
Topics: Abies; Agrobacterium tumefaciens; Anti-Bacterial Agents; Cells, Cultured; Genes, Reporter; Genetic Vectors; Gentamicins; Glucuronidase; Plants, Genetically Modified; Seeds; Selection, Genetic; Transformation, Genetic | 2009 |
Development of K562 cell clones expressing beta-globin mRNA carrying the beta039 thalassaemia mutation for the screening of correctors of stop-codon mutations.
Topics: beta-Globins; beta-Thalassemia; Clone Cells; Cloning, Molecular; Codon, Nonsense; Gene Expression; Gentamicins; Green Fluorescent Proteins; Humans; K562 Cells; Lentivirus; Mutagenesis, Site-Directed; Point Mutation; Polymerase Chain Reaction; Recombinant Fusion Proteins; RNA, Messenger; Transcription, Genetic; Transfection | 2009 |
Aminoglycoside antibiotics restore functional expression of truncated HERG channels produced by nonsense mutations.
Topics: Analysis of Variance; Blotting, Western; Cells, Cultured; Codon, Nonsense; ERG1 Potassium Channel; Ether-A-Go-Go Potassium Channels; Gentamicins; Humans; Long QT Syndrome; Microscopy, Confocal; Patch-Clamp Techniques; Transfection | 2009 |
Expression of GFP using Pichia pastoris vectors with zeocin or G-418 sulphate as the primary selectable marker.
Topics: Anti-Infective Agents; Antibiotics, Antineoplastic; Bleomycin; Cloning, Molecular; Drug Resistance, Fungal; Genetic Engineering; Genetic Vectors; Gentamicins; Green Fluorescent Proteins; Microbial Sensitivity Tests; Pichia; Recombinant Proteins; Selection, Genetic; Transformation, Genetic | 2009 |
Stop codon read-through of a methylmalonic aciduria mutation.
Topics: Adenine; Chromosomes, Artificial, Bacterial; Cisplatin; Codon, Terminator; Genes, Reporter; Gentamicins; HeLa Cells; Humans; Metabolism, Inborn Errors; Methylmalonyl-CoA Mutase; Mutation, Missense; Organophosphonates; RNA, Messenger; Zidovudine | 2009 |
Antiviral activity of geneticin against dengue virus.
Topics: Animals; Antiviral Agents; Cell Line; Cricetinae; Cytopathogenic Effect, Viral; Dengue Virus; Gentamicins; Inhibitory Concentration 50; Viral Plaque Assay; Yellow fever virus | 2009 |
Multiple antibiotic resistance in Arabidopsis is conferred by mutations in a chloroplast-localized transport protein.
Topics: Amino Acid Sequence; Arabidopsis; Arabidopsis Proteins; Chloroplasts; Chromosome Mapping; Cloning, Molecular; DNA, Bacterial; Drug Resistance, Multiple; Edetic Acid; Ferrous Compounds; Gentamicins; Homeostasis; Iron; Iron Deficiencies; Membrane Proteins; Models, Biological; Molecular Sequence Data; Mutagenesis, Insertional; Mutant Proteins; Mutation; Phenotype; Plants, Genetically Modified; Protein Transport; Recombinant Fusion Proteins; Saccharomyces cerevisiae | 2009 |
Regulation of ANKRD9 expression by lipid metabolic perturbations.
Topics: Amebicides; Amino Acid Sequence; Animals; Ankyrin Repeat; Apoptosis; Chick Embryo; Chickens; Fatty Acids, Nonesterified; Gene Expression Profiling; Gene Expression Regulation, Developmental; Gentamicins; Lipid Metabolism; Liver; Membrane Transport Proteins; Molecular Sequence Data; Oligonucleotide Array Sequence Analysis; Proteins; Reverse Transcriptase Polymerase Chain Reaction; RNA, Messenger; Sequence Homology, Amino Acid | 2009 |
Production of beta-globin and adult hemoglobin following G418 treatment of erythroid precursor cells from homozygous beta(0)39 thalassemia patients.
Topics: beta-Globins; beta-Thalassemia; Cells, Cultured; Codon, Nonsense; Erythroid Precursor Cells; Gentamicins; Hemoglobins; Homozygote; Humans; K562 Cells | 2009 |
A novel, drug-based, cellular assay for the activity of neurotoxic mutants of the prion protein.
Topics: Amebicides; Animals; Antibiotics, Antineoplastic; Biological Assay; Bleomycin; Cell Line; Cell Survival; Cinnamates; Drug Evaluation, Preclinical; Gentamicins; GPI-Linked Proteins; Humans; Hygromycin B; Mice; Mice, Transgenic; Phenotype; Prion Diseases; Prions; Protein Isoforms; PrPC Proteins; PrPSc Proteins; Stem Cells | 2010 |
Going green in Cryptococcus neoformans: the recycling of a selectable drug marker.
Topics: Chromosomes, Fungal; Cryptococcus neoformans; DNA, Fungal; Fungal Proteins; Gene Deletion; Gene Expression Regulation, Fungal; Genetic Markers; Genetic Vectors; Genome, Fungal; Gentamicins; Integrases; Molecular Sequence Data; Multigene Family; Mutagenesis, Insertional; Phenotype; Recombinant Fusion Proteins | 2010 |
A novel kanamycin/G418 resistance marker for direct selection of transformants in Escherichia coli and different yeast species.
Topics: Escherichia coli; Fungal Proteins; Genetic Markers; Gentamicins; Kanamycin; Transformation, Genetic; Yeasts | 2010 |
ATP-binding cassette transporters as pitfalls in selection of transgenic cells.
Topics: Animals; ATP Binding Cassette Transporter, Subfamily B, Member 1; ATP-Binding Cassette Transporters; Cell Line, Transformed; Cinnamates; Dogs; Gentamicins; Humans; Hygromycin B; Puromycin; RNA, Messenger; Transfection; Transgenes | 2010 |
Repairing faulty genes by aminoglycosides: development of new derivatives of geneticin (G418) with enhanced suppression of diseases-causing nonsense mutations.
Topics: Aminoglycosides; Animals; Codon, Nonsense; Cystic Fibrosis; Drug Design; Genetic Diseases, Inborn; Genetic Techniques; Gentamicins; Humans; Mucopolysaccharidosis I; Muscular Dystrophy, Duchenne; Trisaccharides; Usher Syndromes | 2010 |
Agrobacterium-mediated transformation of Euphorbia tirucalli callus.
Topics: Carbenicillin; Euphorbia; Gene Expression Regulation; Gentamicins; Rhizobium; Transfection | 2010 |
Entamoeba histolytica: differential gene expression during programmed cell death and identification of early pro- and anti-apoptotic signals.
Topics: Amebicides; Amino Acid Sequence; Amino Acyl-tRNA Synthetases; Amplified Fragment Length Polymorphism Analysis; Apoptosis; Calcium-Binding Proteins; DNA, Complementary; Entamoeba histolytica; Gene Expression; Gentamicins; Polymerase Chain Reaction; Protozoan Proteins; Saposins; Sequence Alignment; Sirtuins | 2010 |
Disruption of gap junctions attenuates aminoglycoside-elicited renal tubular cell injury.
Topics: Aminoglycosides; Androstadienes; Animals; Blotting, Western; Cell Communication; Cell Survival; Cinnamates; Connexin 43; Cytoprotection; Dose-Response Relationship, Drug; Epithelial Cells; Fluorescent Antibody Technique; Gap Junctions; Gentamicins; Glycyrrhetinic Acid; Hygromycin B; Kidney Tubules; LLC-PK1 Cells; Phosphorylation; Protein Kinase Inhibitors; Proto-Oncogene Proteins c-akt; Rats; Recombinant Fusion Proteins; RNA Interference; Swine; Transfection; Wortmannin | 2010 |
An antibiotic selection marker for nematode transgenesis.
Topics: Animals; Animals, Genetically Modified; Caenorhabditis; Drug Resistance; Genetic Markers; Genetic Vectors; Gentamicins; Neomycin; Selection, Genetic; Transgenes | 2010 |
C. elegans select.
Topics: Animals; Animals, Genetically Modified; Caenorhabditis elegans; Drug Resistance; Gentamicins; Selection, Genetic; Transgenes | 2010 |
Budding yeast Mph1 promotes sister chromatid interactions by a mechanism involving strand invasion.
Topics: 4-Nitroquinoline-1-oxide; Coccidiostats; DEAD-box RNA Helicases; DNA Damage; DNA-Directed DNA Polymerase; Drug Resistance; Gentamicins; Mutation; Rad51 Recombinase; Rad52 DNA Repair and Recombination Protein; Saccharomyces cerevisiae; Saccharomyces cerevisiae Proteins; Sister Chromatid Exchange | 2011 |
Adeno-associated viral vector-mediated expression of NT4-ADNF-9 fusion gene protects against aminoglycoside-induced auditory hair cell loss in vitro.
Topics: Aminoglycosides; Animals; Anti-Bacterial Agents; Artificial Gene Fusion; Cell Culture Techniques; Chick Embryo; Dependovirus; Ganglia, Spinal; Genetic Vectors; Gentamicins; Hair Cells, Auditory; Nerve Growth Factors; Oligopeptides; Rats; Transfection | 2011 |
Prospective identification and isolation of enteric nervous system progenitors using Sox2.
Topics: Animals; Biomarkers; Cell Separation; Cells, Cultured; Enteric Nervous System; Gene Expression Profiling; Gentamicins; Hirschsprung Disease; Humans; Immunohistochemistry; Mice; Neural Crest; Neural Stem Cells; Neurogenesis; SOXB1 Transcription Factors; Stem Cell Transplantation | 2011 |
Nonsense suppressor therapies rescue peroxisome lipid metabolism and assembly in cells from patients with specific PEX gene mutations.
Topics: Alleles; Animals; CHO Cells; Chondrodysplasia Punctata, Rhizomelic; Codon, Nonsense; Cricetinae; Cricetulus; Female; Fibroblasts; Gentamicins; Humans; Lipid Metabolism; Membrane Proteins; Oxadiazoles; Peroxisomal Biogenesis Factor 2; Peroxisomal Disorders; Peroxisomal Targeting Signal 2 Receptor; Plasmalogens; Receptors, Cytoplasmic and Nuclear | 2011 |
Toward genetic transformation of mitochondria in mammalian cells using a recoded drug-resistant selection marker.
Topics: Animals; Base Sequence; Cell Line; Cell Proliferation; Cell Survival; DNA, Mitochondrial; Drug Resistance; Gene Order; Gentamicins; Kanamycin Kinase; Mice; Mitochondria; Molecular Sequence Data; Transfection | 2011 |
Live imaging of cell extrusion from the epidermis of developing zebrafish.
Topics: Actins; Animals; Animals, Genetically Modified; Apoptosis; Epidermal Cells; Epidermis; Gentamicins; Green Fluorescent Proteins; Luminescent Proteins; Microscopy, Confocal; Red Fluorescent Protein; Zebrafish | 2011 |
A high G418-resistant neo(R) transgenic mouse and mouse embryonic fibroblast (MEF) feeder layers for cytotoxicity and gene targeting in vivo and in vitro.
Topics: Animals; Blotting, Southern; Cell Survival; Cells, Cultured; Drug Resistance; Feeder Cells; Fibroblasts; Gene Targeting; Gentamicins; Kanamycin Kinase; Lethal Dose 50; Mice; Mice, Inbred C57BL; Mice, Transgenic; Plasmids; Promoter Regions, Genetic; Toxicity Tests, Acute; Transgenes | 2011 |
The viral RNA-based transfection of enhanced green fluorescent protein (EGFP) in the parasitic protozoan Trichomonas vaginalis.
Topics: Animals; Drug Resistance; Female; Genetic Vectors; Gentamicins; Green Fluorescent Proteins; Humans; Microscopy, Fluorescence; Reverse Transcriptase Polymerase Chain Reaction; RNA, Viral; Transfection; Trichomonas vaginalis; Trichomonas Vaginitis | 2012 |
Construction and characterization of hepatocyte nuclear factor HNF4alpha1 over-expressing cell line derived from human hepatoma HepG2 cells.
Topics: Albumins; alpha 1-Antitrypsin; Antiprotozoal Agents; Cell Line, Tumor; Cell Survival; Cytochrome P-450 CYP1A1; Cytochrome P-450 CYP3A; Fibrinogen; Gentamicins; Glyceraldehyde-3-Phosphate Dehydrogenases; Hepatocyte Nuclear Factor 4; Hepatocytes; Humans; Plasminogen; Pregnane X Receptor; Receptors, Aryl Hydrocarbon; Receptors, Steroid; Reverse Transcriptase Polymerase Chain Reaction; Transfection | 2011 |
Functional characterization and targeted correction of ATM mutations identified in Japanese patients with ataxia-telangiectasia.
Topics: Aminoglycosides; Asian People; Ataxia Telangiectasia; Ataxia Telangiectasia Mutated Proteins; Base Sequence; Cell Cycle Proteins; Cell Line; Codon, Nonsense; DNA Mutational Analysis; DNA-Binding Proteins; Exons; Frameshift Mutation; Gentamicins; Heterozygote; Humans; Molecular Sequence Data; Molecular Targeted Therapy; Morpholinos; Oligodeoxyribonucleotides, Antisense; Pedigree; Protein Serine-Threonine Kinases; RNA Splicing; Sequence Deletion; T-Lymphocytes; Tumor Suppressor Proteins | 2012 |
A homozygous mutant embryonic stem cell bank applicable for phenotype-driven genetic screening.
Topics: Animals; Biological Specimen Banks; Embryonic Stem Cells; Genomics; Gentamicins; Homozygote; Mice; Mutation; Phenotype; Polymorphism, Single Nucleotide; Protein Tyrosine Phosphatase, Non-Receptor Type 11; Proteins; Puromycin; RNA-Binding Proteins; Tissue Banks | 2011 |
A structural basis for the antibiotic resistance conferred by an A1408G mutation in 16S rRNA and for the antiprotozoal activity of aminoglycosides.
Topics: Amebicides; Anti-Bacterial Agents; Bacteria; Crystallography, X-Ray; Drug Resistance, Microbial; Gentamicins; Models, Molecular; Mutation; RNA, Bacterial; RNA, Ribosomal, 16S | 2012 |
Determination of the optimal concentration of several selective drugs useful for generating multi-transgenic porcine embryonic fibroblasts.
Topics: Acetyltransferases; Animals; Animals, Genetically Modified; Bleomycin; Cell Death; DNA; Drug Resistance; Female; Fibroblasts; Genetic Markers; Gentamicins; Gestational Age; Green Fluorescent Proteins; Hygromycin B; Nuclear Transfer Techniques; Nucleosides; Puromycin; Swine; Swine, Miniature; Transfection; Transgenes | 2012 |
An antibiotic selection marker for schistosome transgenesis.
Topics: Animals; Anthelmintics; Cell Survival; Culture Media; Gene Transfer Techniques; Gentamicins; Molecular Biology; Organisms, Genetically Modified; Retroviridae; Schistosoma mansoni; Selection, Genetic | 2012 |
Establishment of ultra long-lived cell lines by transfection of TERT into normal human fibroblast TIG-1 and their characterization.
Topics: Abnormal Karyotype; Animals; Cell Line; Cell Shape; Cell Survival; Cell Transformation, Neoplastic; Cyclin-Dependent Kinase Inhibitor p21; Female; Fibroblasts; Gentamicins; Humans; Mice; Mice, SCID; Recombinant Proteins; Telomerase; Telomere Homeostasis; Transfection; Tumor Suppressor Protein p53 | 2012 |
Characterization of a compensatory mutant of Leishmania major that lacks ether lipids but exhibits normal growth, and G418 and hygromycin resistance.
Topics: Acyltransferases; Animals; Antiprotozoal Agents; Cinnamates; Drug Resistance; Gene Amplification; Gentamicins; Glycosphingolipids; Hygromycin B; Leishmania major; Membrane Proteins; Metalloendopeptidases; Mice; Mice, Inbred BALB C; Mutation; Phospholipid Ethers; Proteoglycans; Protozoan Proteins; Virulence | 2012 |
Multiple gene deletion in Cryptococcus neoformans using the Cre-lox system.
Topics: Attachment Sites, Microbiological; Bacteriophage P1; Cryptococcus neoformans; Gene Deletion; Genetic Markers; Gentamicins; Integrases; Mutagenesis, Site-Directed | 2012 |
Use of in vivo and in vitro systems to select Leishmania amazonensis expressing green fluorescent protein.
Topics: Amebicides; Animals; Flow Cytometry; Gentamicins; Green Fluorescent Proteins; Host-Parasite Interactions; Leishmania mexicana; Leishmaniasis, Cutaneous; Luminescent Agents; Macrophages, Peritoneal; Mice; Mice, Inbred BALB C; Organisms, Genetically Modified; Spectrometry, Fluorescence | 2011 |
Genetic transformation of Pseudochoricystis ellipsoidea, an aliphatic hydrocarbon-producing green alga.
Topics: Blotting, Southern; Chlorophyta; Cloning, Molecular; DNA, Plant; Gene Expression Regulation, Plant; Genes, Plant; Genetic Engineering; Genetic Markers; Genetic Vectors; Gentamicins; Hydrocarbons; Kanamycin Kinase; Plants, Genetically Modified; Plasmids; Promoter Regions, Genetic; Transformation, Genetic | 2012 |
Translational infidelity-induced protein stress results from a deficiency in Trm9-catalyzed tRNA modifications.
Topics: Base Pairing; Base Sequence; Codon; Gene Knockout Techniques; Gentamicins; Peptide Elongation Factors; Protein Biosynthesis; Protein Synthesis Inhibitors; Ribonucleotide Reductases; RNA Processing, Post-Transcriptional; RNA, Transfer; Saccharomyces cerevisiae; Saccharomyces cerevisiae Proteins; Stress, Physiological; tRNA Methyltransferases; Unfolded Protein Response | 2012 |
Epigenetically heritable alteration of fly development in response to toxic challenge.
Topics: Animals; Basic Helix-Loop-Helix Transcription Factors; Drosophila melanogaster; Drosophila Proteins; Embryonic Development; Epigenesis, Genetic; Female; Gene Expression Regulation, Developmental; Gentamicins; Inheritance Patterns; Male; Models, Animal; Phenotype; Polycomb-Group Proteins; Promoter Regions, Genetic; Protein Synthesis Inhibitors; Repressor Proteins; Stress, Physiological | 2012 |
Germline transgenesis and insertional mutagenesis in Schistosoma mansoni mediated by murine leukemia virus.
Topics: Animals; Animals, Genetically Modified; DNA, Helminth; Drug Resistance; Female; Gene Transfer Techniques; Gentamicins; High-Throughput Nucleotide Sequencing; Kanamycin Kinase; Leukemia Virus, Murine; Mice; Molecular Sequence Data; Mutagenesis, Insertional; Ovum; Schistosoma mansoni; Snails; Transgenes | 2012 |
Stable transfection of the diplomonad parasite Spironucleus salmonicida.
Topics: Acyltransferases; Aminohydrolases; Animals; Blotting, Western; Chromosomes; Cloning, Molecular; Diplomonadida; Escherichia coli; Genetic Markers; Genetic Vectors; Gentamicins; Glutathione Transferase; Hemagglutinins; Inhibitory Concentration 50; Kanamycin Kinase; Microscopy, Confocal; Nucleosides; Organisms, Genetically Modified; Plasmids; Porins; Promoter Regions, Genetic; Puromycin; Recombinant Proteins; Transfection | 2012 |
Construction and characterization of peroxisome proliferator-activated receptor-gamma co-activator 1 alpha (PGC-1α over-expressing cell line derived from human hepatocyte carcinoma HepG2 cells).
Topics: Basic Helix-Loop-Helix Transcription Factors; Biomarkers, Tumor; Cytochrome P-450 CYP1A1; Cytochrome P-450 CYP3A; Fibrinogen; Gentamicins; Hep G2 Cells; Hepatocyte Nuclear Factor 4; Hepatocytes; Humans; Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha; Polychlorinated Dibenzodioxins; PPAR gamma; Pregnane X Receptor; Receptors, Aryl Hydrocarbon; Receptors, Steroid; Teratogens; Transcription Factors; Transfection | 2013 |
Germline transformation of the silkworm Bombyx mori L. by sperm-mediated gene transfer.
Topics: Animal Husbandry; Animals; Animals, Genetically Modified; Anti-Bacterial Agents; Bombyx; DNA Transposable Elements; Drug Resistance; Female; Gene Transfer Techniques; Genes, Insect; Gentamicins; Green Fluorescent Proteins; Larva; Male; Neomycin; Nerve Tissue Proteins; Nucleopolyhedroviruses; Recombinant Fusion Proteins; Spermatozoa; Transformation, Genetic | 2012 |
Generation of Trichoderma atroviride mutants with constitutively activated G protein signaling by using geneticin resistance as selection marker.
Topics: Anti-Infective Agents; Bacterial Proteins; Biological Control Agents; Escherichia coli; Fungal Proteins; Gene Expression; Genetic Engineering; Genome, Fungal; Gentamicins; GTP-Binding Proteins; Hygromycin B; Kanamycin Kinase; Mutation; Signal Transduction; Transformation, Genetic; Trichoderma | 2012 |
STIM2 drives Ca2+ oscillations through store-operated Ca2+ entry caused by mild store depletion.
Topics: Animals; Calcium; Calcium Channels; Calcium Signaling; Cell Adhesion Molecules; Drosophila; Endoplasmic Reticulum; Gene Silencing; Gentamicins; HEK293 Cells; Humans; Mast Cells; Membrane Proteins; Neoplasm Proteins; ORAI1 Protein; RNA, Small Interfering; Stromal Interaction Molecule 1; Stromal Interaction Molecule 2; T-Lymphocytes | 2013 |
In vitro read-through of phenylalanine hydroxylase (PAH) nonsense mutations using aminoglycosides: a potential therapy for phenylketonuria.
Topics: Aminoglycosides; Animals; Chlorocebus aethiops; Codon, Nonsense; COS Cells; Drug Evaluation, Preclinical; Genetic Therapy; Gentamicins; HEK293 Cells; Humans; Oxadiazoles; Phenylalanine Hydroxylase; Phenylketonurias | 2013 |
High error rates in selenocysteine insertion in mammalian cells treated with the antibiotic doxycycline, chloramphenicol, or geneticin.
Topics: Amebicides; Amino Acid Substitution; Animals; Anti-Bacterial Agents; Arginine; Cell Line, Tumor; Chloramphenicol; Doxycycline; Gentamicins; Glutathione Peroxidase; Glutathione Peroxidase GPX1; Humans; Mice; Phospholipid Hydroperoxide Glutathione Peroxidase; RNA, Transfer, Amino Acid-Specific; Selenocysteine; Selenoproteins; Thioredoxins | 2013 |
Splice variants DNMT3B4 and DNMT3B7 overexpression inhibit cell proliferation in 293A cell line.
Topics: Blotting, Western; Cell Proliferation; Cyclin-Dependent Kinase Inhibitor p21; DNA (Cytosine-5-)-Methyltransferases; DNA Methylation; DNA Methyltransferase 3B; DNA Primers; Flow Cytometry; Gene Expression Regulation; Gene Silencing; Gentamicins; HEK293 Cells; Humans; Plasmids; Protein Isoforms; Real-Time Polymerase Chain Reaction; Tetrazolium Salts; Thiazoles | 2013 |
A lack of premature termination codon read-through efficacy of PTC124 (Ataluren) in a diverse array of reporter assays.
Topics: Animals; beta-Galactosidase; Biological Assay; Cell Line; Codon, Nonsense; Collagen Type VII; Genes, Reporter; Gentamicins; Humans; Luciferases, Firefly; Oxadiazoles; Transfection | 2013 |
The glyceraldehyde-3-phosphate dehydrogenase promoter of the food yeast Candida utilis strain NRRL Y-660 is functional in Agrobacterium tumefaciens.
Topics: Agrobacterium tumefaciens; Candida; Cloning, Molecular; DNA, Fungal; Genetic Vectors; Gentamicins; Glucuronidase; Glyceraldehyde-3-Phosphate Dehydrogenases; Kanamycin; Promoter Regions, Genetic; Transformation, Genetic | 2013 |
Identification of the molecular attributes required for aminoglycoside activity against Leishmania.
Topics: Crystallization; Gentamicins; Leishmania; Leishmaniasis; Models, Molecular; Molecular Structure; Nebramycin; Protein Binding; Protein Conformation; Ribosomal Proteins | 2013 |
Entamoeba histolytica P-glycoprotein (EhPgp) inhibition, induce trophozoite acidification and enhance programmed cell death.
Topics: Amebicides; Antisense Elements (Genetics); Apoptosis; ATP Binding Cassette Transporter, Subfamily B, Member 1; Entamoeba histolytica; Gene Expression; Gentamicins; Hydrogen-Ion Concentration; Plasmids; Transfection; Trophozoites | 2013 |
The L-cell isolation from heterogonous population of intestinal cell line using antibiotic selection method.
Topics: Animals; Anti-Bacterial Agents; Cell Line, Tumor; Cell Separation; Enteroendocrine Cells; Gentamicins; Glucagon-Like Peptide 1; Intestines; Mice; Plasmids; Real-Time Polymerase Chain Reaction; Reverse Transcriptase Polymerase Chain Reaction; Transfection | 2014 |
Repair of UV photolesions in xeroderma pigmentosum group C cells induced by translational readthrough of premature termination codons.
Topics: Codon, Nonsense; DNA Primers; DNA Repair; Enzyme-Linked Immunosorbent Assay; Fibroblasts; Fluorescent Antibody Technique; Gentamicins; Humans; Immunoblotting; Luciferases; Oxadiazoles; Real-Time Polymerase Chain Reaction; RNA Stability; Skin Neoplasms; Xeroderma Pigmentosum | 2013 |
Growth arrest on inhibition of nonsense-mediated decay is mediated by noncoding RNA GAS5.
Topics: Codon, Nonsense; Gene Expression Regulation; Gentamicins; Humans; Jurkat Cells; Lymphocytes; Protein Biosynthesis; RNA Helicases; RNA Stability; RNA, Long Noncoding; RNA, Small Interfering; TOR Serine-Threonine Kinases; Trans-Activators; Transcriptome | 2013 |
Reduction in maternal Polycomb levels contributes to transgenerational inheritance of a response to toxic stress in flies.
Topics: Amebicides; Animals; Drosophila melanogaster; Drosophila Proteins; Epigenesis, Genetic; Female; Gene Expression Regulation, Developmental; Gentamicins; Larva; Polycomb Repressive Complex 1; Stress, Physiological | 2014 |
Identification of Tf1 integration events in S. pombe under nonselective conditions.
Topics: Base Sequence; Drug Resistance, Fungal; Gene Expression Regulation, Fungal; Gene Silencing; Genome, Fungal; Gentamicins; Hydroxamic Acids; Molecular Sequence Data; Retroelements; RNA, Fungal; Schizosaccharomyces; Selection, Genetic | 2014 |
Use of the tumor repressor DEDD as a prognostic marker of cancer metastasis.
Topics: Biomarkers, Tumor; Cell Line, Tumor; Death Domain Receptor Signaling Adaptor Proteins; Drug Resistance, Neoplasm; Gene Knockdown Techniques; Gene Silencing; Gentamicins; Humans; Immunohistochemistry; Immunoprecipitation; Neoplasm Metastasis; Prognosis; Proteolysis; Transfection; Tumor Suppressor Proteins; Two-Hybrid System Techniques | 2014 |
Genetic engineering combined with random mutagenesis to enhance G418 production in Micromonospora echinospora.
Topics: Fermentation; Genetic Engineering; Gentamicins; Micromonospora; Mutagenesis | 2014 |
Silencing the wild-type and mutant K-ras increases the resistance to 5-flurouracil in HCT-116 as a colorectal cancer cell line.
Topics: Antimetabolites, Antineoplastic; Apoptosis; Cell Proliferation; Down-Regulation; Drug Resistance, Neoplasm; Fluorouracil; Gene Expression Regulation, Neoplastic; Gene Silencing; Genes, ras; Gentamicins; HCT116 Cells; Humans; Mutation; Real-Time Polymerase Chain Reaction | 2015 |
ECG in neonate mice with spinal muscular atrophy allows assessment of drug efficacy.
Topics: Animals; Animals, Newborn; Biomarkers; Bradycardia; Disease Models, Animal; Drug Evaluation, Preclinical; Electrocardiography; Gentamicins; Heart; Heart Block; Heart Conduction System; Mice; Motor Activity; Muscular Atrophy, Spinal; Random Allocation; Toxicity Tests | 2015 |
Readthrough of stop codons by use of aminoglycosides in cells from xeroderma pigmentosum group C patients.
Topics: Administration, Topical; Aminoglycosides; Cells, Cultured; Codon, Nonsense; DNA-Binding Proteins; Gene Expression; Gentamicins; Humans; Mutant Proteins; Precision Medicine; RNA, Messenger; Xeroderma Pigmentosum | 2015 |
Enhancing T-DNA Transfer Efficiency in Barley (Hordeum vulgare L.) Cells Using Extracellular Cellulose and Lectin.
Topics: Acetophenones; Agrobacterium tumefaciens; Cellulose; DNA, Bacterial; DNA, Single-Stranded; Gene Expression; Gene Transfer Techniques; Genetic Vectors; Gentamicins; Hordeum; Kanamycin Kinase; Lectins; Plants, Genetically Modified; Transgenes | 2015 |
A Universal Positive-Negative Selection System for Gene Targeting in Plants Combining an Antibiotic Resistance Gene and Its Antisense RNA.
Topics: Alleles; Blotting, Southern; Chromosome Segregation; Drug Resistance, Microbial; Gene Knockout Techniques; Gene Targeting; Genes, Plant; Genetic Loci; Gentamicins; Oryza; Phenotype; Plants, Genetically Modified; RNA, Antisense; RNA, Messenger; Transcription, Genetic | 2015 |
A novel TXNIP-based mechanism for Cx43-mediated regulation of oxidative drug injury.
Topics: Aminoglycosides; Animals; Carrier Proteins; Cell Cycle Proteins; Cell Death; Cell Line; Connexin 43; Doxorubicin; Gap Junctions; Gentamicins; Mice; Oxidation-Reduction; Oxidative Stress; Phosphorylation; Proto-Oncogene Proteins c-akt; Puromycin; Rats; Signal Transduction | 2015 |
Novel Properties for Endoglucanase Acquired by Cell-Surface Display Technique.
Topics: Cell Surface Display Techniques; Cellulase; Congo Red; Enzyme Stability; Gene Expression; Gentamicins; Hydrogen-Ion Concentration; Pichia; Recombinant Fusion Proteins; Saccharomyces cerevisiae; Temperature; Transformation, Genetic | 2015 |
Limited premature termination codon suppression by read-through agents in cystic fibrosis intestinal organoids.
Topics: Cells, Cultured; Coccidiostats; Codon, Nonsense; Cystic Fibrosis; Cystic Fibrosis Transmembrane Conductance Regulator; Gentamicins; Humans; Organoids; Oxadiazoles; RNA | 2016 |
Evaluation of Aminoglycoside and Non-Aminoglycoside Compounds for Stop-Codon Readthrough Therapy in Four Lysosomal Storage Diseases.
Topics: Animals; Cell Line; Chlorocebus aethiops; Codon, Nonsense; Codon, Terminator; COS Cells; Fibroblasts; Gentamicins; Humans; Lysosomes; Mucopolysaccharidosis III; Mucopolysaccharidosis VI; Niemann-Pick Disease, Type A; Niemann-Pick Disease, Type B; RNA, Messenger | 2015 |
Development of Gateway Binary Vector Series with Four Different Selection Markers for the Liverwort Marchantia polymorpha.
Topics: Acetolactate Synthase; Acetyltransferases; Agrobacterium tumefaciens; Bacterial Proteins; Genetic Markers; Genetic Vectors; Gentamicins; Kanamycin Kinase; Marchantia; Plants, Genetically Modified; Sulfonamides; Transfection; Triazines | 2015 |
Calpain-like: A Ca(2+) dependent cystein protease in Entamoeba histolytica cell death.
Topics: Amebicides; Amino Acid Sequence; Blotting, Western; Calcium; Calpain; Cysteine Endopeptidases; Cysteine Proteinase Inhibitors; Densitometry; DNA Fragmentation; Entamoeba histolytica; Gene Expression Regulation, Enzymologic; Gentamicins; In Situ Nick-End Labeling; Leucine; Leupeptins; Microscopy, Confocal; Molecular Sequence Data; Real-Time Polymerase Chain Reaction | 2015 |
Development of a system for multicopy gene integration in Saccharomyces cerevisiae.
Topics: Alkaline Phosphatase; Base Sequence; Chromosomes, Artificial, Yeast; Drug Resistance, Microbial; Enzyme Assays; Escherichia coli; Gene Amplification; Gene Expression; Genes, Reporter; Genetic Vectors; Gentamicins; Molecular Sequence Data; Mutagenesis, Insertional; Real-Time Polymerase Chain Reaction; Saccharomyces cerevisiae; Saccharomyces cerevisiae Proteins; Transformation, Genetic | 2016 |
Geneticin Stabilizes the Open Conformation of the 5' Region of Hepatitis C Virus RNA and Inhibits Viral Replication.
Topics: Antiviral Agents; Cell Line, Tumor; Codon, Initiator; Gentamicins; Hepacivirus; Humans; Kinetics; Nucleic Acid Conformation; Ribonuclease III; Ribonuclease P; RNA, Viral; Viral Nonstructural Proteins; Virus Replication | 2016 |
Investigation on the partial resistance of Cpkk2 knock out strain of Cryphonectria parasitica to Cryphonectria hypovirus 1 infection in presence of Geneticin and Geneticin resistance gene.
Topics: Ascomycota; Fungal Viruses; Gene Deletion; Gene Knockout Techniques; Genes, Fungal; Gentamicins | 2016 |
IPEC-J2 MDR1, a Novel High-Resistance Cell Line with Functional Expression of Human P-glycoprotein (ABCB1) for Drug Screening Studies.
Topics: Amebicides; Animals; Antimetabolites, Antineoplastic; ATP Binding Cassette Transporter, Subfamily B; ATP Binding Cassette Transporter, Subfamily B, Member 1; Blotting, Western; Caco-2 Cells; Cell Membrane Permeability; Drug Resistance, Multiple; Gentamicins; Humans; Immunoenzyme Techniques; Intestinal Mucosa; Puromycin; Substrate Specificity; Swine | 2016 |
Constitutive Store-Operated Ca(2+) Entry Leads to Enhanced Nitric Oxide Production and Proliferation in Infantile Hemangioma-Derived Endothelial Colony-Forming Cells.
Topics: Anilides; Calcium; Cell Proliferation; Child; Child, Preschool; Colony-Forming Units Assay; Demography; Endothelial Cells; Endothelial Progenitor Cells; Female; Gene Expression Regulation; Gentamicins; Hemangioma; Humans; Indoles; Intracellular Space; Lanthanum; Male; Nitric Oxide; RNA, Messenger; Thiadiazoles | 2016 |
[Expression of mannose-binding lectin associated protein 19 (MAp19) in HeLa cells].
Topics: Blotting, Western; Cell Survival; Cloning, Molecular; Dose-Response Relationship, Drug; Gene Expression; Genetic Vectors; Gentamicins; HeLa Cells; Humans; Liposomes; Mannose-Binding Protein-Associated Serine Proteases; Microscopy, Fluorescence; Recombinant Fusion Proteins; Transfection | 2016 |
An Atypical System for Studying Epithelial-Mesenchymal Transition in Hepatocellular Carcinoma.
Topics: Animals; Antigens, CD; Cadherins; Carcinoma, Hepatocellular; Cell Line, Tumor; Cellular Reprogramming; Epithelial-Mesenchymal Transition; Gentamicins; Heterografts; Humans; Liver Neoplasms; MAP Kinase Signaling System; Mice, Nude; Neoplastic Stem Cells; Signal Transduction; Transcription Factors; Transforming Growth Factor beta; Vimentin | 2016 |
Development of an Efficient Genome Editing Method by CRISPR/Cas9 in a Fish Cell Line.
Topics: Animals; Animals, Genetically Modified; Bacterial Proteins; Base Sequence; Cell Line; Cinnamates; Cloning, Molecular; Clustered Regularly Interspaced Short Palindromic Repeats; CRISPR-Associated Protein 9; CRISPR-Cas Systems; Endonucleases; Gene Editing; Genes, Reporter; Genetic Engineering; Genetic Vectors; Gentamicins; Green Fluorescent Proteins; Hygromycin B; Plasmids; Salmon; Transfection | 2016 |
Development of a toolbox to dissect host-endosymbiont interactions and protein trafficking in the trypanosomatid Angomonas deanei.
Topics: Animals; Base Sequence; Betaproteobacteria; Cinnamates; Genetic Vectors; Genome, Protozoan; Genomics; Gentamicins; Green Fluorescent Proteins; Homologous Recombination; Hygromycin B; Mutagenesis, Insertional; Protein Transport; Protozoan Proteins; Reproducibility of Results; Sequence Analysis, DNA; Subcellular Fractions; Symbiosis; Transcriptome; Trypanosomatina | 2016 |
Specific factor IX mRNA and protein features favor drug-induced readthrough over recurrent nonsense mutations.
Topics: Amino Acid Substitution; Arginine; Codon, Nonsense; Factor IX; Gene Expression Regulation; Gentamicins; HEK293 Cells; Hemophilia B; Humans; Protein Biosynthesis; Recombinant Proteins; Tryptophan | 2017 |
Rescue of the MERTK phagocytic defect in a human iPSC disease model using translational read-through inducing drugs.
Topics: Adult; c-Mer Tyrosine Kinase; Gentamicins; Humans; Induced Pluripotent Stem Cells; Male; Oxadiazoles; Peptide Chain Elongation, Translational; Phagocytosis; Photoreceptor Cells; Retinal Pigment Epithelium; Retinitis Pigmentosa | 2017 |
Colonic organoids derived from human induced pluripotent stem cells for modeling colorectal cancer and drug testing.
Topics: Adenoma; Adenomatous Polyposis Coli; Adenomatous Polyposis Coli Protein; Antibiotics, Antineoplastic; Blotting, Western; Cell Differentiation; Cell Proliferation; Colon; Colorectal Neoplasms; Drug Screening Assays, Antitumor; Enteroendocrine Cells; Flow Cytometry; Fluorescent Antibody Technique; Gene Expression Profiling; Gentamicins; Germ-Line Mutation; Goblet Cells; Heterocyclic Compounds, 3-Ring; Human Embryonic Stem Cells; Humans; Immunohistochemistry; Induced Pluripotent Stem Cells; Microscopy, Confocal; Mutation; Organoids; Real-Time Polymerase Chain Reaction; Sirolimus; Wnt Signaling Pathway | 2017 |
[Gene optimization and efficient expression of Trichoderma reesei Cel5A in Pichia pastoris].
Topics: Cellulase; Cellulose; Cellulose 1,4-beta-Cellobiosidase; Codon; Fermentation; Fungal Proteins; Gene Expression; Gentamicins; Industrial Microbiology; Pichia; Recombinant Proteins; Temperature; Trichoderma | 2016 |
A structural basis for the antibiotic resistance conferred by an N1-methylation of A1408 in 16S rRNA.
Topics: Adenosine; Aminoglycosides; Binding Sites; Drug Resistance, Bacterial; Gentamicins; Methylation; Models, Molecular; Mutation; Paromomycin; RNA, Bacterial; RNA, Ribosomal, 16S | 2017 |
Collection of homozygous mutant mouse embryonic stem cells arising from autodiploidization during haploid gene trap mutagenesis.
Topics: Animals; Cells, Cultured; Diploidy; DNA Transposable Elements; Flow Cytometry; Genetic Vectors; Gentamicins; Haploidy; Homozygote; Mice; Mouse Embryonic Stem Cells; Mutagenesis; Poly A; Puromycin; Reproducibility of Results | 2018 |
Readthrough of ACTN3 577X nonsense mutation produces full-length α-actinin-3 protein.
Topics: Actinin; Caffeine; Codon, Nonsense; Gentamicins; HEK293 Cells; Humans; Muscle, Skeletal; Mutant Proteins; Peptide Chain Termination, Translational; RNA Stability; RNA, Messenger; Transfection | 2018 |
Establishment of pantropic spotted dolphin (Stenella attenuata) fibroblast cell line and potential influence of polybrominated diphenyl ethers (PBDEs) on cytokines response.
Topics: Animals; Cell Line; Cell Proliferation; Cryopreservation; Cytokines; Dermis; Female; Fibroblasts; Gentamicins; Halogenated Diphenyl Ethers; Karyotype; Polybrominated Biphenyls; Stenella; Transfection; Vimentin; Water Pollutants, Chemical | 2018 |
Sleeping beauty transposon-mediated poly(A)-trapping and insertion mutagenesis in mouse embryonic stem cells.
Topics: Animals; Cell Line; Cell Self Renewal; DNA Transposable Elements; Doxycycline; Drug Resistance; Genetic Vectors; Gentamicins; Mice; Mouse Embryonic Stem Cells; Mutagenesis, Insertional; Poly A; ras GTPase-Activating Proteins; Transcription, Genetic; Transcriptional Activation | 2018 |
A Calpain-Like Protein Is Involved in the Execution Phase of Programmed Cell Death of
Topics: Apoptosis; Calpain; Computational Biology; Entamoeba histolytica; Enzyme Activators; Gentamicins; Protein Interaction Maps | 2018 |
The aminoglycoside geneticin permits translational readthrough of the CTNS W138X nonsense mutation in fibroblasts from patients with nephropathic cystinosis.
Topics: Amino Acid Transport Systems, Neutral; Codon, Nonsense; Cystine; Cystinosis; Fibroblasts; Genetic Vectors; Gentamicins; HEK293 Cells; Humans; Peptide Chain Termination, Translational; Plasmids; Recombinant Proteins; RNA, Messenger; Transfection | 2019 |
G418 induces programmed cell death in Acanthamoeba through the elevation of intracellular calcium and cytochrome c translocation.
Topics: Acanthamoeba; Amebicides; Apoptosis; Calcium; Cell Nucleus; Cytochromes c; Gentamicins; Humans; Mitochondria | 2019 |
A breakthrough in readthrough? Could geneticin lead the way to effective treatment for cystinosis nonsense mutations?
Topics: Amino Acid Transport Systems, Neutral; Aminoglycosides; Codon, Nonsense; Cystinosis; Fibroblasts; Gentamicins; Humans | 2019 |
Targeting Translation Termination Machinery with Antisense Oligonucleotides for Diseases Caused by Nonsense Mutations.
Topics: Animals; Codon, Nonsense; Disease Models, Animal; Factor IX; Gentamicins; Hemophilia A; Humans; Liver; Mice; Molecular Targeted Therapy; Oligonucleotides, Antisense; Peptide Chain Termination, Translational; Peptide Termination Factors; Protein Biosynthesis; RNA, Messenger | 2019 |
The antimalarial drug mefloquine enhances TP53 premature termination codon readthrough by aminoglycoside G418.
Topics: Antimalarials; Codon, Nonsense; Codon, Terminator; Gentamicins; HCT116 Cells; Humans; Mefloquine; Peptide Chain Termination, Translational; Tumor Suppressor Protein p53 | 2019 |
Agrobacterium-mediated transformation of Ceratocystis albifundus.
Topics: Agrobacterium tumefaciens; Ascomycota; Blotting, Southern; Carbenicillin; Coculture Techniques; DNA, Bacterial; Gene Expression Regulation, Fungal; Gentamicins; Green Fluorescent Proteins; Hygromycin B; Kanamycin; Polymerase Chain Reaction; Sequence Analysis; Transformation, Genetic; Virulence | 2019 |
N-acetyltransferase AAC(3)-I confers gentamicin resistance to Phytophthora palmivora and Phytophthora infestans.
Topics: Arylamine N-Acetyltransferase; Drug Resistance; Fluorescent Dyes; Gentamicins; Isoenzymes; Phytophthora; Phytophthora infestans; Plant Diseases; Transformation, Genetic | 2019 |
Serum starvation enhances nonsense mutation readthrough.
Topics: Adenomatous Polyposis Coli Protein; Anti-Bacterial Agents; Cell Line; Codon, Nonsense; Codon, Terminator; Culture Media; Genes, APC; Gentamicins; Green Fluorescent Proteins; HEK293 Cells; Humans; Mutation; Protein Biosynthesis; Serum | 2019 |
Structural Bases for the Fitness Cost of the Antibiotic-Resistance and Lethal Mutations at Position 1408 of 16S rRNA.
Topics: Anti-Bacterial Agents; Base Pairing; Crystallography, X-Ray; Drug Resistance, Microbial; Gentamicins; Models, Molecular; Mutation; Nucleic Acid Conformation; Ribosomes; RNA, Messenger; RNA, Ribosomal, 16S; RNA, Transfer | 2019 |
Chemical Modifications Reduce Auditory Cell Damage Induced by Aminoglycoside Antibiotics.
Topics: Anti-Bacterial Agents; Cell Membrane Permeability; Gentamicins; Hair Cells, Auditory; Humans; Mitochondria; Molecular Dynamics Simulation; Nebramycin; Proteins; Ribosomes | 2020 |
Nonsense suppression induced readthrough of a novel PAX6 mutation in patient-derived cells of congenital aniridia.
Topics: Adult; Aniridia; Cells, Cultured; Child; Female; Gentamicins; Haploinsufficiency; Heterozygote; Humans; INDEL Mutation; Male; Middle Aged; Nonsense Mediated mRNA Decay; Oxadiazoles; PAX6 Transcription Factor; Pedigree | 2020 |
Two dominant selectable markers for genetic manipulation in Neurospora crassa.
Topics: Acetyltransferases; Anti-Bacterial Agents; DNA Transposable Elements; Drug Resistance, Fungal; Gene Expression Regulation, Fungal; Gene Knockout Techniques; Genes, Dominant; Genetic Markers; Gentamicins; Kanamycin Kinase; Microorganisms, Genetically-Modified; Neurospora crassa; Promoter Regions, Genetic; Quinic Acid; Streptothricins | 2020 |
Premature termination codon readthrough upregulates progranulin expression and improves lysosomal function in preclinical models of GRN deficiency.
Topics: Animals; Cells, Cultured; Codon, Nonsense; Codon, Terminator; Frontotemporal Lobar Degeneration; Gene Expression; Gentamicins; HEK293 Cells; Humans; Lysosomes; Mice; Neurons; Progranulins; Up-Regulation | 2020 |
A drug-inducible sex-separation technique for insects.
Topics: Animals; Drosophila melanogaster; Drosophila Proteins; Drug Resistance; Exons; Female; Genetic Engineering; Genetics, Population; Gentamicins; Homozygote; Introns; Male; Pest Control; Puromycin; RNA Splicing; Sex Determination Analysis | 2020 |
Pharmacological read-through of R294X Mecp2 in a novel mouse model of Rett syndrome.
Topics: Animals; Brain; Disease Models, Animal; Gentamicins; Humans; Methyl-CpG-Binding Protein 2; Mice; Mutation; Neurodevelopmental Disorders; Phenotype; Rett Syndrome | 2020 |
Chemical modifications of G418 (geneticin): Synthesis of novel readthrough aminoglycosides results in an improved in vitro safety window but no improvements in vivo.
Topics: Aminoglycosides; Animals; Gentamicins; Hair; Molecular Conformation; Rats; Zebrafish | 2020 |
Dihydropyriculol produced by Pyricularia oryzae inhibits the growth of Streptomyces griseus.
Topics: Anti-Bacterial Agents; Antibiosis; Ascomycota; Benzaldehydes; Cycloheximide; Fatty Alcohols; Gentamicins; Hygromycin B; Microbial Sensitivity Tests; Secondary Metabolism; Streptomyces griseus; Toxins, Biological | 2021 |
Small molecule Y-320 stimulates ribosome biogenesis, protein synthesis, and aminoglycoside-induced premature termination codon readthrough.
Topics: Aminoglycosides; Cell Line; Chemokines, CXC; Codon, Nonsense; Codon, Terminator; Gentamicins; Humans; Mutation; Protein Biosynthesis; Protein Synthesis Inhibitors; Ribosomes | 2021 |
A small molecule that induces translational readthrough of CFTR nonsense mutations by eRF1 depletion.
Topics: Aminoglycosides; Codon, Nonsense; Cystic Fibrosis Transmembrane Conductance Regulator; Epithelial Cells; Genes, Reporter; Gentamicins; HEK293 Cells; Humans; Microsomes, Liver; Nonsense Mediated mRNA Decay; Peptide Chain Termination, Translational; Peptide Termination Factors; Proteasome Endopeptidase Complex; Ribosomes; RNA Interference; Structure-Activity Relationship | 2021 |
The aminoglycoside G418 hinders de novo prion infection in cultured cells.
Topics: Aminoglycosides; Animals; Cell Line; Cell Line, Tumor; Gentamicins; Mice; Prion Diseases; Prion Proteins; Prions; Protein Synthesis Inhibitors; PrPC Proteins; PrPSc Proteins | 2021 |
Multiplexed drug-based selection and counterselection genetic manipulations in Drosophila.
Topics: Animals; Animals, Genetically Modified; Chromosomes, Artificial, Bacterial; Drosophila; Drug Resistance; Female; Ganciclovir; Genetic Techniques; Gentamicins; Male; Transgenes | 2021 |
A standard knockout procedure alters expression of adjacent loci at the translational level.
Topics: 3' Untranslated Regions; 5' Untranslated Regions; Base Sequence; Codon; Gene Expression Regulation, Fungal; Gene Knockout Techniques; Genes, Reporter; Genetic Loci; Gentamicins; Green Fluorescent Proteins; Open Reading Frames; Protein Biosynthesis; Ribosomes; Saccharomyces cerevisiae; Sequence Deletion; Transcription Initiation Site; Transcription, Genetic | 2021 |
Optimizing Lipofectamine LTX Complex and G-418 Concentration for Improvement of Transfection Efficiency in Human Mesenchymal Stem Cells.
Topics: Gentamicins; Humans; Lipids; Mesenchymal Stem Cells; Transfection | 2021 |
Geneticin reduces mRNA stability.
Topics: Amino Acids; Animals; Codon; Gentamicins; Mice; Protein Biosynthesis; RNA Stability; RNA, Messenger | 2022 |