anoplin and 1-2-dioleoyl-sn-glycero-3-phosphoglycerol

In order to enhance the membrane disruption of antimicrobial peptides both targeting and multivalent presentation approaches were explored. The antimicrobial peptides anoplin and temporin L were conjugated via click chemistry to vancomycin and to di- and tetravalent dendrimers. The vancomycin unit led to enhanced membrane disruption of large unilamellar vesicles (LUVs) displaying the vancomycin target lipid II, but only for temporin L and not for anoplin. The multivalent presentation led to enhanced LUV membrane disruption in the case of anoplin but not for temporin L.

Topics: Antimicrobial Cationic Peptides; Biophysics; Chromatography, High Pressure Liquid; Drug Design; Fluoresceins; Humans; Lipids; Models, Chemical; Peptides; Phosphatidylcholines; Phosphatidylglycerols; Proteins; Vancomycin; Wasp Venoms