anisomycin and oxophenylarsine

It has been proposed that growth cones navigating through gradients adapt to baseline concentrations of guidance cues. This adaptation process is poorly understood. Using the collapse assay, we show that adaptation in Xenopus laevis retinal growth cones to the guidance cues Sema3A or netrin-1 involves two processes: a fast, ligand-specific desensitization that occurs within 2 min of exposure and is dependent on endocytosis, and a slower, ligand-specific resensitization, which occurs within 5 min and is dependent upon protein synthesis. These two phases of adaptation allow retinal axons to adjust their range of sensitivity to specific guidance cues.

Topics: Adaptation, Biological; Animals; Anisomycin; Arsenicals; Cell Adhesion Molecules; Cells, Cultured; Chemokine CCL22; Chemokines, CC; Chlorocebus aethiops; COS Cells; Cycloheximide; Dose-Response Relationship, Drug; Drug Interactions; Endocytosis; Fluorescent Antibody Technique; Growth Cones; In Vitro Techniques; Nerve Growth Factors; Netrin-1; Neurons; Neuropilin-1; Protein Synthesis Inhibitors; Retina; Semaphorin-3A; Statistics, Nonparametric; Time Factors; Transfection; Tumor Suppressor Proteins; Xenopus laevis