angiotensin-i and 2--deoxy-5--adenosine-monophosphate

We report on the applicability of combining surface-enhanced Raman scattering (SERS) with coherent anti-Stokes Raman scattering for high-sensitivity detection of biological molecules. We found that this combination of techniques provides more than 3 orders of signal enhancement compared with SERS and permits monitoring of biological molecules such as deoxyguanosine monophosphate (dGMP) and deoxyadenosine monophosphate at the single-molecule level. This combined technique also improved detection sensitivity for angiotensin peptide. As this is believed to be the first report of detection of dGMP at the single-molecule level, we suggest that this approach can serve as a new tool for biological studies.

Topics: Angiotensin I; Biopolymers; Deoxyadenine Nucleotides; Molecular Biology; Molecular Probe Techniques; Spectrum Analysis, Raman; Surface Plasmon Resonance; Tomography, Optical Coherence