angiogenin and bromoacetate

angiogenin has been researched along with bromoacetate* in 1 studies

Other Studies

1 other study(ies) available for angiogenin and bromoacetate

Article	Year
Sites of modification of human angiogenin by bromoacetate at pH 5.5. Biochemical and biophysical research communications, 1988, Oct-14, Volume: 156, Issue:1 Human angiogenin is inactivated by treatment with bromoacetate at pH 5.5. Use of [14C]bromoacetate and tryptic peptide mapping have identified the sites of carboxymethylation as His-13 and His-114, with His-114 reacting approximately 1.5-fold more rapidly than His-13. At later stages in the reaction, both His-13 and -114 become modified with His-114 in part forming a bis derivative. Comparison with carboxymethylhistidine derivatives of known structure obtained from bovine pancreatic ribonuclease A indicates that the reaction order is N-1 of His-114 greater than N-3 of His-13 greater than N-3 of His-114. Topics: Acetates; Amino Acids; Angiogenesis Inducing Agents; Binding Sites; Chromatography, High Pressure Liquid; Growth Substances; Humans; Kinetics; Neoplasm Proteins; Peptide Fragments; Protein Binding; Ribonuclease, Pancreatic; Trypsin	1988

Article

Year

Sites of modification of human angiogenin by bromoacetate at pH 5.5.

Biochemical and biophysical research communications, 1988, Oct-14, Volume: 156, Issue:1

Human angiogenin is inactivated by treatment with bromoacetate at pH 5.5. Use of [14C]bromoacetate and tryptic peptide mapping have identified the sites of carboxymethylation as His-13 and His-114, with His-114 reacting approximately 1.5-fold more rapidly than His-13. At later stages in the reaction, both His-13 and -114 become modified with His-114 in part forming a bis derivative. Comparison with carboxymethylhistidine derivatives of known structure obtained from bovine pancreatic ribonuclease A indicates that the reaction order is N-1 of His-114 greater than N-3 of His-13 greater than N-3 of His-114.

Topics: Acetates; Amino Acids; Angiogenesis Inducing Agents; Binding Sites; Chromatography, High Pressure Liquid; Growth Substances; Humans; Kinetics; Neoplasm Proteins; Peptide Fragments; Protein Binding; Ribonuclease, Pancreatic; Trypsin

1988