amphotericin-b and diphenyleneiodonium

Parsley cells recognize the fungal plant pathogen Phytophthora sojae through a plasma membrane receptor. A pathogen-derived oligopeptide elicitor binds to this receptor and thereby stimulates a multicomponent defense response through sequential activation of ion channels and an oxidative burst. An elicitor-responsive mitogen-activated protein (MAP) kinase was identified that acts downstream of the ion channels but independently or upstream of the oxidative burst. Upon receptor-mediated activation, the MAP kinase is translocated to the nucleus where it might interact with transcription factors that induce expression of defense genes.

Topics: Amino Acid Sequence; Amphotericin B; Anthracenes; Calcium-Calmodulin-Dependent Protein Kinases; Cell Nucleus; Cells, Cultured; Enzyme Activation; Fungal Proteins; Ion Channels; Membrane Glycoproteins; Molecular Sequence Data; Onium Compounds; Peptide Fragments; Phosphorylation; Phytophthora; Plants; Respiratory Burst