amphotericin-b and boric-acid

A higher prevalence of vulvovaginal candidiasis (VVC) is seen in pregnant women compared with those who are not pregnant. Recurrence is also more common in pregnant women, and therapeutic responses are reduced. In this investigation, 207 vaginal yeast isolates recovered from pregnant women were tested for susceptibility to 13 antifungal drugs and boric acid and through these studies four virulence factors were also determined. The isolates were recovered from vaginal samples of patients with acute VVC [AVVC, (n = 73)], symptomatic recurrent VVC [RVVC, (n = 89)], asymptomatic RVVC (n = 27), and those without signs and symptoms (n = 18). Candida albicans was the most common species found (59.9%), followed by C. glabrata (19.8%), other Candida spp., (19.8%), and Saccharomyces cerevisiae (0.5%). Antifungal susceptibility testing was performed as described in CLSI document M27-A3. Additionally, we examined phospholipase and proteinase production, adhesion to vaginal epithelial cells and hemolytic activity. Notably, the MIC values of Candida spp. isolates derived from patients with VVC were no different from those of the controls (P > 0.05). In addition, Candida isolates derived from patients with AVVC or RVVC produced significantly higher amounts of phospholipase and proteinase compared with the controls (P < 0.05). Antifungal testing and the determination of virulence factors may lead to the effective and prompt treatment of VVC, particularly in pregnant women.

Topics: Adolescent; Adult; Amphotericin B; Animals; Antifungal Agents; Boric Acids; Candida albicans; Candida glabrata; Candidiasis, Vulvovaginal; Cell Adhesion; Epithelial Cells; Female; Fluconazole; Fungal Proteins; Hemolysis; Humans; Itraconazole; Microbial Sensitivity Tests; Middle Aged; Phospholipases; Pregnancy; Pregnancy Complications, Infectious; Recurrence; Saccharomyces cerevisiae; Vagina; Virulence Factors; Young Adult

Strain differentiation of 66 clinical isolates of Candida albicans obtained from healthy dentate and complete denture wearers was performed. Resistogram method based on differences in the resistance of C. albicans isolates to sodium selenite, boric acid, cetrimide, sodium periodate and silver nitrate was used for strain differentiation. Of the 32 potential strains that can be distinguished, 14 different resistogram strains of C. albicans were found among the 66 isolates tested. Strain-C--was the most predominant (24.3% of total isolates), while strain A-CDE was the least predominant (1.5%). The results showed no particular association of certain strains with Candida infections in complete denture wearers. Sensitivity to antifungal agents showed that isolates from different strains were most sensitive to amphotericin B and nystatin and least sensitive to miconazole.

Topics: Amphotericin B; Antifungal Agents; Boric Acids; Candida albicans; Candidiasis, Oral; Cetrimonium; Cetrimonium Compounds; Denture, Complete; Drug Resistance, Microbial; Humans; Miconazole; Microbial Sensitivity Tests; Nystatin; Periodic Acid; Silver Nitrate; Sodium Selenite; Stomatitis, Denture