amoxicillin-potassium-clavulanate-combination and betadex

A simple, rapid and accurate method for simultaneous determination of amoxycillin and clavulanic acid using HPLC with beta-cyclodextrin stationary phase was developed. It involves the use of tetraethylammonium acetate (TEAA) as an additive reagent, methanol-buffer solution (pH 4.5) (35:65; v/v) as the mobile phase, detection at 225 mm and chromatogram within 12 min. Linearity and precision of the internal standard method have been obtained. Recoveries ranged from 99.25 to 105.63% for amoxycillin in the synthetic mixture. For clavulanic acid it was from 99.50 to 101.64%. This method is convenient and reproducible for analyses of these two components in different dosage forms.

Topics: Amoxicillin; Amoxicillin-Potassium Clavulanate Combination; beta-Cyclodextrins; Buffers; Chromatography, High Pressure Liquid; Clavulanic Acids; Cyclodextrins; Dosage Forms; Drug Therapy, Combination; Hydrogen-Ion Concentration; Spectrophotometry, Ultraviolet