amanitins and thymidine-5--triphosphate

amanitins has been researched along with thymidine-5--triphosphate* in 2 studies

Other Studies

2 other study(ies) available for amanitins and thymidine-5--triphosphate

Article	Year
Sites in human nuclei where damage induced by ultraviolet light is repaired: localization relative to transcription sites and concentrations of proliferating cell nuclear antigen and the tumour suppressor protein, p53. Journal of cell science, 1994, Volume: 107 ( Pt 7) The repair of damage induced in DNA by ultraviolet light involves excision of the damaged sequence and synthesis of new DNA to repair the gap. Sites of such repair synthesis were visualized by incubating permeabilized HeLa or MRC-5 cells with the DNA precursor, biotin-dUTP, in a physiological buffer; then incorporated biotin was immunolabeled with fluorescent antibodies. Repair did not take place at sites that reflected the DNA distribution; rather, sites were focally concentrated in a complex pattern. This pattern changed with time; initially intense repair took place at transcriptionally active sites but when transcription became inhibited it continued at sites with little transcription. Repair synthesis in vitro also occurred in the absence of transcription. Repair sites generally contained a high concentration of proliferating cell nuclear antigen but not the tumour-suppressor protein, p53. Topics: Amanitins; Cell Line; Cell Membrane Permeability; Cell Nucleus; DNA; DNA Damage; DNA Repair; HeLa Cells; Humans; Kinetics; Phosphorus Radioisotopes; Proliferating Cell Nuclear Antigen; Thymine Nucleotides; Transcription, Genetic; Tumor Suppressor Protein p53; Ultraviolet Rays	1994
[Synthesis of RNA and DNA in isolated nuclei of Colpoda cucullus (Protozoi Ciliati)]. Bollettino della Societa italiana di biologia sperimentale, 1983, Aug-30, Volume: 59, Issue:8 The ciliated protozoan Colpoda cucullus has been cultivated at 27 degrees C with gentle shaking in a baked lettuce infusion supplemented with Klebsiella suspensions. Under these conditions cells had a mean generation time of about 7 hours and could attain densities up to 20,000/ml and 45,000/ml in the log and stationary phase of growth, respectively. Nuclear preparations obtained from exponentially growing cells by the gum arabic-octanol method showed a satisfactory degree of purity and integrity. They consisted primarily of the large macronuclei attached to which the small micronuclei were sometimes visible. Upon incubation at 27 degrees C in conventional reaction mixtures nuclear preparations actively incorporated 3H-UTP and 3H-dTTP into acid-insoluble material. alpha-amanitin caused a 50% inhibition of RNA synthesis whereas aphidicolin did not affect at all DNA synthesis. Topics: Amanitins; Animals; Aphidicolin; Cell Nucleus; Ciliophora; Diterpenes; DNA Replication; RNA; Thymine Nucleotides; Uridine Triphosphate	1983

Article

Year

Sites in human nuclei where damage induced by ultraviolet light is repaired: localization relative to transcription sites and concentrations of proliferating cell nuclear antigen and the tumour suppressor protein, p53.

Journal of cell science, 1994, Volume: 107 ( Pt 7)

The repair of damage induced in DNA by ultraviolet light involves excision of the damaged sequence and synthesis of new DNA to repair the gap. Sites of such repair synthesis were visualized by incubating permeabilized HeLa or MRC-5 cells with the DNA precursor, biotin-dUTP, in a physiological buffer; then incorporated biotin was immunolabeled with fluorescent antibodies. Repair did not take place at sites that reflected the DNA distribution; rather, sites were focally concentrated in a complex pattern. This pattern changed with time; initially intense repair took place at transcriptionally active sites but when transcription became inhibited it continued at sites with little transcription. Repair synthesis in vitro also occurred in the absence of transcription. Repair sites generally contained a high concentration of proliferating cell nuclear antigen but not the tumour-suppressor protein, p53.

Topics: Amanitins; Cell Line; Cell Membrane Permeability; Cell Nucleus; DNA; DNA Damage; DNA Repair; HeLa Cells; Humans; Kinetics; Phosphorus Radioisotopes; Proliferating Cell Nuclear Antigen; Thymine Nucleotides; Transcription, Genetic; Tumor Suppressor Protein p53; Ultraviolet Rays

1994

[Synthesis of RNA and DNA in isolated nuclei of Colpoda cucullus (Protozoi Ciliati)].

Bollettino della Societa italiana di biologia sperimentale, 1983, Aug-30, Volume: 59, Issue:8

The ciliated protozoan Colpoda cucullus has been cultivated at 27 degrees C with gentle shaking in a baked lettuce infusion supplemented with Klebsiella suspensions. Under these conditions cells had a mean generation time of about 7 hours and could attain densities up to 20,000/ml and 45,000/ml in the log and stationary phase of growth, respectively. Nuclear preparations obtained from exponentially growing cells by the gum arabic-octanol method showed a satisfactory degree of purity and integrity. They consisted primarily of the large macronuclei attached to which the small micronuclei were sometimes visible. Upon incubation at 27 degrees C in conventional reaction mixtures nuclear preparations actively incorporated 3H-UTP and 3H-dTTP into acid-insoluble material. alpha-amanitin caused a 50% inhibition of RNA synthesis whereas aphidicolin did not affect at all DNA synthesis.

Topics: Amanitins; Animals; Aphidicolin; Cell Nucleus; Ciliophora; Diterpenes; DNA Replication; RNA; Thymine Nucleotides; Uridine Triphosphate

1983