aluminum-phthalocyanine-disulfonate and phthalocyanine

Photodynamic therapy is an attractive technique for various skin tumors and non-cancerous skin lesions. However, while the aim of photodynamic therapy is to target and damage only the malignant cells, it unavoidably affects some of the healthy cells surrounding the tumor as well. However, data on the effects of PDT to normal cells are scarce, and the characterization of the pathways activated after the photodamage of normal cells may help to improve clinical photodynamic therapy. In our study, primary human epidermal keratinocytes were used to evaluate photodynamic treatment effects of photosensitizers with different subcellular localization. We compared the response of keratinocytes to lysosomal photodamage induced by phthalocyanines, aluminum phthalocyanine disulfonate (AlPcS

Topics: Apoptosis; Autophagy; Cell Differentiation; Computer Simulation; Humans; Indoles; Isoindoles; Keratinocytes; Kinetics; Lysosomes; Mesoporphyrins; Models, Biological; Organometallic Compounds; Photochemotherapy; Photosensitizing Agents

Athymic nude mice with human tumors transplanted to one of the hind legs were given aluminium phthalocyanine disulfonate (AlPcS2) intraperitoneally. Twenty-four hours after the injection the mice were placed with the tumor in the sample position in a fluorescence spectrometer with modulated excitation. Exposure of the tumors to laser light at a fluence rate of 50-200 mW/cm2 led to a rapid transient reduction by up to 50% of the phthalocyanine fluorescence of the tumor. After the laser irradiation the fluence rate of the fluorescence increased almost up to the initial value within a few minutes. This finding should be taken into account when optimal fluence rates and dose fractionation schemes are sought for photodynamic therapy.

Topics: Animals; Cell Line; Female; Humans; Indoles; Isoindoles; Melanoma; Mice; Mice, Nude; Neoplasm Transplantation; Organometallic Compounds; Photochemotherapy; Pigments, Biological; Radiation-Sensitizing Agents; Spectrometry, Fluorescence; Transplantation, Heterologous