alpha-synuclein and 3-aminotyrosine

Tyrosine nitration is a post-translational protein modification relevant to various pathophysiological processes. Chemical nitration procedures have been used to generate and study nitrated proteins, but these methods regularly lead to modifications at other amino acid residues. A novel strategy employs a genetic code modification that allows incorporation of 3-nitrotyrosine (3-NT) during ribosomal protein synthesis to generate a recombinant protein with defined 3-NT-sites, in the absence of other post-translational modifications. This approach was applied to study the generation and stability of the 3-NT moiety in recombinant proteins produced in E.coli. Nitrated alpha-synuclein (ASYN) was selected as exemplary protein, relevant in Parkinson's disease (PD). A procedure was established to obtain pure tyrosine-modified ASYN in mg amounts. However, a rapid (t

Topics: alpha-Synuclein; Cloning, Molecular; Escherichia coli; Escherichia coli Proteins; Gene Expression; Genetic Vectors; Green Fluorescent Proteins; Humans; Metabolic Networks and Pathways; Oxidation-Reduction; Protein Engineering; Recombinant Proteins; Tyrosine