alpha-cyperone and n-hexane

alpha-cyperone has been researched along with n-hexane* in 1 studies

Other Studies

1 other study(ies) available for alpha-cyperone and n-hexane

Article	Year
α-Cyperone, isolated from the rhizomes of Cyperus rotundus, inhibits LPS-induced COX-2 expression and PGE2 production through the negative regulation of NFκB signalling in RAW 264.7 cells. Journal of ethnopharmacology, 2013, May-02, Volume: 147, Issue:1 The rhizomes of Cyperus rotundus (Cyperaceae) have been used in Asian traditional medicine for the treatment of several inflammatory diseases. However, the anti-inflammatory effects of α-cyperone, a major active compound of Cyperus rotundus, are poorly understood.. PGE2 and cytokines released from cells were measured using an EIA assay kit. The expression of iNOS, COX-2, TNF-α, and IL-6 was measured by real-time RT-PCR and/or Western blot analysis. A luciferase assay was performed to measure the effect of α-cyperone on NFκB activity.. The n-hexane fraction of the 80% EtOH extract from the rhizomes of Cyperus rotundus was found to inhibit both NO and PGE2 production in RAW 264.7 cells. α-Cyperone isolated from the n-hexane fraction significantly inhibited PGE2 production by suppressing the LPS-induced expression of inducible COX-2 at both the mRNA and the protein levels. In contrast, α-cyperone had little effect on NO production and iNOS expression. Additionally, α-cyperone downregulated the production and mRNA expression of the inflammatory cytokine IL-6. Moreover, treatment with α-cyperone suppressed the transcriptional activity of NFκB and the nuclear translocation of the p65 NFκB subunit in LPS-induced RAW 264.7 cells.. The anti-inflammatory activity of α-cyperone is associated with the down-regulation of COX-2 and IL-6 via the negative regulation of the NFκB pathway in LPS-stimulated RAW 264.7 cells. Topics: Animals; Anti-Inflammatory Agents; Blotting, Western; Cell Line; Cyclooxygenase 2; Cyperus; Dinoprostone; Dose-Response Relationship, Drug; Down-Regulation; Ethanol; Genes, Reporter; Hexanes; Immunoenzyme Techniques; Interleukin-6; Lipopolysaccharides; Macrophages; Medicine, Korean Traditional; Mice; Naphthalenes; NF-kappa B; Nitric Oxide Synthase Type II; Phytotherapy; Plant Extracts; Plants, Medicinal; Real-Time Polymerase Chain Reaction; Reverse Transcriptase Polymerase Chain Reaction; Rhizome; RNA, Messenger; Signal Transduction; Solvents; Transfection; Tumor Necrosis Factor-alpha	2013

Article

Year

α-Cyperone, isolated from the rhizomes of Cyperus rotundus, inhibits LPS-induced COX-2 expression and PGE2 production through the negative regulation of NFκB signalling in RAW 264.7 cells.

Journal of ethnopharmacology, 2013, May-02, Volume: 147, Issue:1

The rhizomes of Cyperus rotundus (Cyperaceae) have been used in Asian traditional medicine for the treatment of several inflammatory diseases. However, the anti-inflammatory effects of α-cyperone, a major active compound of Cyperus rotundus, are poorly understood.. PGE2 and cytokines released from cells were measured using an EIA assay kit. The expression of iNOS, COX-2, TNF-α, and IL-6 was measured by real-time RT-PCR and/or Western blot analysis. A luciferase assay was performed to measure the effect of α-cyperone on NFκB activity.. The n-hexane fraction of the 80% EtOH extract from the rhizomes of Cyperus rotundus was found to inhibit both NO and PGE2 production in RAW 264.7 cells. α-Cyperone isolated from the n-hexane fraction significantly inhibited PGE2 production by suppressing the LPS-induced expression of inducible COX-2 at both the mRNA and the protein levels. In contrast, α-cyperone had little effect on NO production and iNOS expression. Additionally, α-cyperone downregulated the production and mRNA expression of the inflammatory cytokine IL-6. Moreover, treatment with α-cyperone suppressed the transcriptional activity of NFκB and the nuclear translocation of the p65 NFκB subunit in LPS-induced RAW 264.7 cells.. The anti-inflammatory activity of α-cyperone is associated with the down-regulation of COX-2 and IL-6 via the negative regulation of the NFκB pathway in LPS-stimulated RAW 264.7 cells.

Topics: Animals; Anti-Inflammatory Agents; Blotting, Western; Cell Line; Cyclooxygenase 2; Cyperus; Dinoprostone; Dose-Response Relationship, Drug; Down-Regulation; Ethanol; Genes, Reporter; Hexanes; Immunoenzyme Techniques; Interleukin-6; Lipopolysaccharides; Macrophages; Medicine, Korean Traditional; Mice; Naphthalenes; NF-kappa B; Nitric Oxide Synthase Type II; Phytotherapy; Plant Extracts; Plants, Medicinal; Real-Time Polymerase Chain Reaction; Reverse Transcriptase Polymerase Chain Reaction; Rhizome; RNA, Messenger; Signal Transduction; Solvents; Transfection; Tumor Necrosis Factor-alpha

2013