alpha-chymotrypsin and tamoxifen-aziridine

The conformation of estrogen receptor (ER) and its in vitro transformation by RNase, Urea and ATP were analysed using the uteri of young (16 weeks) and old (92 weeks) rats. Following the digestion of ER with proteolytic enzymes like trypsin and chymotrypsin and the analysis of cleaved fragments by SDS-PAGE, similar pattern is observed in both ages. In vitro transformation of ER by RNase, Urea and ATP shows that the degree of transformation is lower in old than young. Furthermore, the transformed ER from old is less capable of binding to DNA than that from young. Thus our results show that the conformation of ER probably does not change with age, but the degree of transformation and the ability of transformed receptor to bind to DNA decrease with age.

Topics: Adenosine Triphosphate; Aging; Animals; Chymotrypsin; DNA; Electrophoresis, Polyacrylamide Gel; Estradiol; Female; Protein Conformation; Rats; Receptors, Estrogen; Ribonucleases; Tamoxifen; Trypsin; Urea; Uterus