alpha-chymotrypsin and salicylaldehyde

A new method for cross-linking of protein was proposed in our previous work. The method is based on the spontaneous chelate formation process involving three components, salicylaldehyde moiety, alpha-amino acid residue and copper(II). In this paper versatility of the method as a purpose of immobilization of enzyme was described. Chymotrypsin-salicylaldehyde conjugate was immobilized to the agarose gel attached alpha-amino acid residue in the presence of copper(II) ion The enzyme was not eluted from the gel by washing with a copper free buffer though it was exclusively eluted by a medium containing EDTA. Catalytic activity of the chymotrypsin salicylaldehyde conjugate was not changed upon the immobilization. The method was proposed as a new tool for reversible immobilization of enzyme.

Topics: Aldehydes; Animals; Catalysis; Cattle; Chelating Agents; Chymotrypsin; Chymotrypsinogen; Copper; Enzyme Activation; Enzymes, Immobilized; Hydrolysis; Kinetics; Schiff Bases