alpha-chymotrypsin and laminaran

alpha-chymotrypsin has been researched along with laminaran* in 1 studies

Other Studies

1 other study(ies) available for alpha-chymotrypsin and laminaran

Article	Year
The role of hemocytes, serine protease inhibitors and pathogen-associated patterns in prophenoloxidase activation in the desert locust, Schistocerca gregaria. Peptides, 2008, Volume: 29, Issue:2 The prophenoloxidase-activating system is an important component of the innate immune response of insects, involved in wound healing and melanotic encapsulation. In this paper we show that in the desert locust, Schistocerca gregaria, hemocytes, challenged with microbial elicitors, are indispensable for the limited proteolytic activation of prophenoloxidase (proPO) in plasma. In addition, we assessed the influence of serine protease inhibitors on the induction of PO-activity in plasma. While soybean Bowman-Birk inhibitor (SBBI) inhibited the PO activation by laminarin-treated hemocytes, the endogenous pacifastin-related inhibitors, SGPI-1 (S. gregaria pacifastin-related inhibitor-1) and SGPI-2 did not affect the PO-activity under similar conditions. On the other hand, real-time PCR analysis revealed that the transcripts, encoding SGPI-1-3, were more abundant in the fat body of immune challenged animals, as compared to control animals. Topics: Animals; Catechol Oxidase; Chymotrypsin; Dose-Response Relationship, Drug; Enzyme Activation; Enzyme Precursors; Fat Body; Female; Gene Expression; Glucans; Grasshoppers; Hemocytes; Hemolymph; Host-Pathogen Interactions; Humans; Insect Proteins; Lipopolysaccharides; Models, Biological; Peptides; Polysaccharides; Serine Proteinase Inhibitors; Trypsin Inhibitor, Bowman-Birk Soybean; Trypsin Inhibitors	2008

Article

Year

The role of hemocytes, serine protease inhibitors and pathogen-associated patterns in prophenoloxidase activation in the desert locust, Schistocerca gregaria.

Peptides, 2008, Volume: 29, Issue:2

The prophenoloxidase-activating system is an important component of the innate immune response of insects, involved in wound healing and melanotic encapsulation. In this paper we show that in the desert locust, Schistocerca gregaria, hemocytes, challenged with microbial elicitors, are indispensable for the limited proteolytic activation of prophenoloxidase (proPO) in plasma. In addition, we assessed the influence of serine protease inhibitors on the induction of PO-activity in plasma. While soybean Bowman-Birk inhibitor (SBBI) inhibited the PO activation by laminarin-treated hemocytes, the endogenous pacifastin-related inhibitors, SGPI-1 (S. gregaria pacifastin-related inhibitor-1) and SGPI-2 did not affect the PO-activity under similar conditions. On the other hand, real-time PCR analysis revealed that the transcripts, encoding SGPI-1-3, were more abundant in the fat body of immune challenged animals, as compared to control animals.

Topics: Animals; Catechol Oxidase; Chymotrypsin; Dose-Response Relationship, Drug; Enzyme Activation; Enzyme Precursors; Fat Body; Female; Gene Expression; Glucans; Grasshoppers; Hemocytes; Hemolymph; Host-Pathogen Interactions; Humans; Insect Proteins; Lipopolysaccharides; Models, Biological; Peptides; Polysaccharides; Serine Proteinase Inhibitors; Trypsin Inhibitor, Bowman-Birk Soybean; Trypsin Inhibitors

2008