alpha-chymotrypsin has been researched along with acryloyl-chloride* in 3 studies
3 other study(ies) available for alpha-chymotrypsin and acryloyl-chloride
| Article | Year |
|---|---|
Just one word: plastics.
Topics: Acrylates; Chymotrypsin; Enzymes, Immobilized; Plastics; Solubility; Solvents; Subtilisins | 1997 |
Enzymatic peptide synthesis in organic solvent mediated by gels of copolymerized acrylic derivatives of alpha-chymotrypsin and polyoxyethylene.
Copolymers of acrylated derivatives of alpha-chymotrypsin and polyethylene glycol (PEG) have been prepared and used as biocatalysts for the synthesis of model peptides in organic solvent containing a low quantity of water. Other peptide couplings have been tried to point out the chemico- and stereoselectivity and examples of segment couplings are given. Topics: Acrylates; Acylation; Catalysis; Chymotrypsin; Peptide Biosynthesis; Peptides; Polyethylene Glycols; Polymers; Stereoisomerism | 1991 |
[The heterogeneous character of protein modification during substitution of their amino groups. Acylation of alpha-chymotrypsin].
Thin-layer isoelectric focusing of chymotrypsin modified by stepwise acylation with acryloyl chloride or maleic anhydride revealed a high heterogeneity of modification products, with a maximal number of components near 50% of substituted amino groups. Disc electrophoresis failed to establish the products diversity and could not therefore be used for heterogeneity control. The activity of the modified enzyme towards proteins and low molecular weight substrates depended on the modification reagent and correlated with the electrostatic enzyme--substrate interaction. The low hydrolytic activity towards N-acetyl-L-tyrosine p-nitroanilide was due to the increase in the Michaelis constant; the value of the catalytic constant remained unchanged. Topics: Acrylates; Acylation; Chymotrypsin; Kinetics; Maleic Anhydrides | 1983 |