alpha-chymotrypsin and 3-3-5-trimethylcyclohexanol

We sought to verify whether isoflavin-beta (Iso-β), a mixture of isoflavones with antioxidant properties, could prevent thyrotoxicosis-induced loss of muscle mass and the participation of oxidative stress (OS) in the mechanisms of this prevention.. Two experimental periods of thyrotoxicosis induction were used in Wistar rats: 3 and 5 days to assess Iso-β effects before and after thyrotoxicosis-induced muscle wasting. After euthanasia, peritoneal fat and gastrocnemius muscle were collected, weighed, and muscle OS was assessed.. Iso-β prevented the loss of gastrocnemius mass in thyrotoxic rats through the prevention of muscle OS generation during thyrotoxicosis, increasing muscle total antioxidant capacity and decreasing mitochondrial cytochrome c oxidase activity, lipid peroxidation, and protein carbonyl content.. Iso-β decreased oxidative modification of proteins, which is known to exert a major role during proteolysis induction and is present in thyrotoxic myopathy, highlighting the potential action of Iso-β in this complication of the disease. Muscle Nerve 56: 975-981, 2017.

Topics: Animals; Antioxidants; Chymotrypsin; Cyclohexanols; Disease Models, Animal; Drug Administration Schedule; Electron Transport Complex IV; Glycerol; Isoflavones; Male; Muscle, Skeletal; Muscular Atrophy; Oxidative Stress; Protein Carbonylation; Rats; Rats, Wistar; Superoxide Dismutase; tert-Butylhydroperoxide; Thiobarbituric Acid Reactive Substances; Thyrotoxicosis