alcohol-oxidase has been researched along with glyoxylic-acid* in 1 studies
1 other study(ies) available for alcohol-oxidase and glyoxylic-acid
| Article | Year |
|---|---|
Expression of alcohol oxidase gene from Ochrobactrum sp. AIU 033 in recombinant Escherichia coli through the twin-arginine translocation pathway.
We cloned a set of genes encoding alcohol oxidase from Ochrobactrum sp. AIU 033 (OcAOD), which exhibits the appropriate substrate specificity for glyoxylic acid production from glycolic acid. The set of genes for OcAOD contained two open reading frames consisting of 555-bp (aodB) and 1572-bp (aodA) nucleotides, which encode the precursor for the β-subunit and α-subunit of OcAOD, respectively. We expressed the cloned genes as an active product in Escherichia coli BL21(DE3). The recombinant OcAOD oxidized glycolic acid and primary alcohols with C2-C8 but not glyoxylic acid (as is the case for native OcAOD), whereas the K Topics: Alcohol Oxidoreductases; Arginine; Biological Transport; Cloning, Molecular; Escherichia coli; Gene Expression Regulation, Bacterial; Gene Expression Regulation, Enzymologic; Glyoxylates; Metabolic Engineering; Metabolic Networks and Pathways; Ochrobactrum; Organisms, Genetically Modified; Periplasm; Protein Subunits; Recombinant Proteins; Sequence Analysis, DNA | 2019 |