alcohol-oxidase and 3-3--5-5--tetramethylbenzidine

The peroxidase-like activity of hollow Prussian Blue nanocubes (hPBNCs) is used, in combination with the enzyme alcohol oxidase (AOx), in a colorimetric ethanol assay. Different from other nanozymes, the large cavity structure of the hPBNCs provides a larger surface and more binding sites for AOx to be bound on their surface or in the pores. This extremely enhances the sensitivity of the assay system. In the presence of ethanol, AOx is capable of catalyzing the oxidation of alcohols to aldehydes, accompanied by the generation of hydrogen peroxide (H

Topics: Alcohol Oxidoreductases; Animals; Benzidines; Biomimetic Materials; Colorimetry; Ethanol; Ferrocyanides; Hydrogen Peroxide; Limit of Detection; Male; Mice, Inbred C57BL; Nanostructures; Oxidation-Reduction; Peroxidase