alanine and guanosine triphosphate

alanine has been researched along with guanosine triphosphate in 48 studies

Research

Studies (48)

TimeframeStudies, this research(%)All Research%
pre-19907 (14.58)18.7374
1990's16 (33.33)18.2507
2000's21 (43.75)29.6817
2010's3 (6.25)24.3611
2020's1 (2.08)2.80

Authors

AuthorsStudies
Irving, MG; Williams, JF2
Kawakami, M; Takemura, S; Tanada, S1
Kyprianou, N; Taylor-Papadimitriou, J1
Bartfai, T; Iverfeldt, K; Ogren, SO1
Gupta, SL; Lengyel, P; Sopori, ML; Waterson, J1
Golini, F; Thach, RE1
Marcus, A; Seal, SN1
Meredith, M; Metz, S; Rabaglia, M1
Bocchini, V; Krab, IM; Parmeggiani, A; Scarano, G1
Lee, YH; Lin, K; Okar, D; Pilkis, SJ1
Castle, D; Dent, P; Romero, G; Sturgill, TW; Vincent, LA; Wu, J1
Boekhoff, I; Breer, H; Restrepo, D1
Coley, C; Daniell, S; Naylor, LH; Strange, PG; Woodward, R1
Ahmadian, MR; Blechschmidt, B; Kreutzer, R; Sprinzl, M1
Cerione, RA; Erickson, JW; Mittal, R1
Hwang, MC; Hwang, YW; Sung, YJ1
Cheng, E; Cline, RT; Fraser, CM; Geoghagen, NS; Lee, NH1
Hoshino, S; Kanaho, Y; Katada, T; Kukimoto, I; Maehama, T; Nimota, K; Nishina, H; Takahashi, K1
Berghuis, AM; Gilman, AG; Lee, E; Raw, AS; Sprang, SR1
Dev, KK; Henley, JM; Roberts, PJ1
Finken-Eigen, M; Köhrer, K; Müller, S1
Liu, X; Malbon, CC; Wang, HY1
Das, S; Maitra, U1
Dagher, PC1
Brannetti, B; Ferrè, F; Helmer-Citterich, M; Valencia, A; Via, A1
Buckingham, R; Dinçbas-Renqvist, V; Ehrenberg, M; Engström, A; Heurgué-Hamard, V; Mora, L1
Bhatt, A; Lin, B; Maddock, JR; Pawloski, L; Pfeffer, SM; Skidmore, JM1
Pleiss, JA; Uhlenbeck, OC1
Gudkov, AT; Liljas, A; Martemyanov, KA; Yarunin, AS1
Barends, S; Karzai, AW; Kraal, B; Sauer, RT; Wower, J1
Brieba, LG; Padilla, R; Sousa, R1
Bearne, SL; Iyengar, A1
Frank, J; Gillet, R; Henne, A; Kaur, S; Ramakrishnan, V; Valle, M1
Bae, YS; Kim, YS; Lee, JY; Park, W; Son, MY1
Krishnamurthy, G; Smith, D; Tawa, G; Wang, Y; Young, KH1
Alves, I; Cowell, S; Davis, P; Hruby, VJ; Lee, YS; Porreca, F; Tang, X1
Leonard, M; Schmid, SL; Song, BD1
Fekete, CA; Hinnebusch, AG; Nielsen, KH; Valásek, L; Zhang, F1
Bar-Sagi, D; Boykevisch, S; Ford, B; Nassar, N; Skowronek, K1
Buschauer, A; Dove, S; Ghorai, P; Kraus, A; Preuss, H; Seifert, R1
Marco-Marín, C; Rubio, V1
Ledoux, S; Olejniczak, M; Uhlenbeck, OC1
Homma, M; Kojima, S; Kusumoto, A; Nishioka, N1
Gambaryan, S; Sickmann, A; Subramanian, H; Walter, U; Zahedi, RP1
Proud, CG; Stead, RL1
Ho, J; Ikura, M; Ishiyama, N; Marshall, CB; Mazhab-Jafari, MT; Stambolic, V1
Elfiky, AA1

Other Studies

48 other study(ies) available for alanine and guanosine triphosphate

ArticleYear
Class experiment: Kinetic studies on the regulation of rabbit liver pyruvate kinase.
    Comparative biochemistry and physiology. B, Comparative biochemistry, 1975, Aug-15, Volume: 51, Issue:4

    Topics: Adenosine Diphosphate; Alanine; Allosteric Regulation; Animals; Chromatography, DEAE-Cellulose; Enzyme Activation; Female; Fructosephosphates; Guanosine Triphosphate; Hexosediphosphates; Hydrogen-Ion Concentration; Isoenzymes; Kinetics; Liver; Magnesium; Mathematics; Pyruvate Kinase; Rabbits

1975
Properties of alanyl-oligonucleotide, puromycin, and Staphylococcus epidermidis glycyl-tRNA in interaction with elongation factor Tu:GTP complex.
    FEBS letters, 1975, Mar-01, Volume: 51, Issue:1

    Topics: Alanine; Binding Sites; Candida; Chromatography, Gel; Glycine; Guanosine Triphosphate; Peptide Elongation Factors; Protein Binding; Puromycin; RNA, Bacterial; RNA, Transfer; Staphylococcus

1975
Isolation of azatyrosine-induced revertants from ras-transformed human mammary epithelial cells.
    Oncogene, 1992, Volume: 7, Issue:1

    Topics: Alanine; Animals; Blotting, Northern; Breast; Cell Division; Cell Transformation, Viral; Cells, Cultured; Genes, ras; Guanosine Diphosphate; Guanosine Triphosphate; Humans; Mice; Mice, Nude; Oncogene Protein p21(ras); RNA, Messenger

1992
Substance P receptors in the rat spinal cord: the effect of GTP and of chronic antidepressant treatment.
    Acta physiologica Scandinavica, 1988, Volume: 132, Issue:2

    Topics: Alanine; Animals; Antidepressive Agents; Guanosine Triphosphate; Imipramine; Rats; Receptors, Neurokinin-1; Receptors, Neurotransmitter; Spinal Cord; Zimeldine

1988
Apparent changes in ribosome conformation during protein synthesis. Centrifugation at high speed distorts initiation, pretranslocaton, and posttranslocation complexes to a different extent.
    Biochemistry, 1972, Apr-11, Volume: 11, Issue:8

    Topics: Alanine; Bacillus; Bacterial Proteins; Bacteriophages; Carbon Isotopes; Centrifugation, Density Gradient; Chromatography, Gel; Escherichia coli; Formates; Fusidic Acid; Guanosine Triphosphate; Methionine; Peptide Biosynthesis; Peptide Chain Elongation, Translational; Peptide Chain Initiation, Translational; Puromycin; Ribosomes; RNA, Transfer; RNA, Viral; Thyrotropin; Tritium

1972
An investigation of the decoding of triplets adjacent to AUG during initiation.
    Biochemical and biophysical research communications, 1972, Jun-28, Volume: 47, Issue:6

    Topics: Alanine; Bacterial Proteins; Carbon Isotopes; Centrifugation, Density Gradient; Escherichia coli; Formaldehyde; Formates; Genetic Code; Guanine Nucleotides; Guanosine Triphosphate; Methionine; Peptide Biosynthesis; Peptide Chain Initiation, Translational; Ribosomes; RNA, Bacterial; RNA, Transfer; RNA, Viral; Serine; Tritium

1972
Kinetic studies on the regulation of rabbit liver pyruvate kinase.
    The Biochemical journal, 1973, Volume: 131, Issue:2

    Topics: Adenosine Diphosphate; Adenosine Triphosphate; Alanine; Allosteric Regulation; Animals; Chromatography, DEAE-Cellulose; Chromatography, Gel; Chromatography, Ion Exchange; Electrophoresis, Polyacrylamide Gel; Enzyme Activation; Female; Fructosephosphates; Guanosine Triphosphate; Hexosediphosphates; Hydrogen-Ion Concentration; Isoenzymes; Kinetics; Liver; Magnesium; Phosphoenolpyruvate; Potassium; Pyruvate Kinase; Rabbits; Spectrophotometry, Ultraviolet

1973
Translation of the initial codons of satellite tobacco necrosis virus ribonucleic acid in a cell-free system from wheat embryo.
    The Journal of biological chemistry, 1973, Oct-10, Volume: 248, Issue:19

    Topics: Adenosine Triphosphate; Alanine; Carbon Radioisotopes; Cell-Free System; Centrifugation, Density Gradient; Chlortetracycline; Chromatography, Paper; Dipeptides; Guanosine Triphosphate; Lysine; Magnesium; Methionine; Peptide Chain Initiation, Translational; Peptide Elongation Factors; Peptide Initiation Factors; Plant Cells; Plants; Protein Biosynthesis; Ribosomes; RNA, Messenger; RNA, Transfer; RNA, Viral; Satellite Viruses; Transfer RNA Aminoacylation; Triticum

1973
Cytosolic biosynthesis of GTP and ATP in normal rat pancreatic islets.
    Biochimica et biophysica acta, 1995, Apr-06, Volume: 1266, Issue:1

    Topics: Adenosine Triphosphate; Alanine; Animals; Cytosol; Guanosine Triphosphate; Insulin; Islets of Langerhans; Male; Mercaptopurine; Mycophenolic Acid; Rats; Rats, Sprague-Dawley

1995
Relevance of histidine-84 in the elongation factor Tu GTPase activity and in poly(Phe) synthesis: its substitution by glutamine and alanine.
    FEBS letters, 1995, May-29, Volume: 365, Issue:2-3

    Topics: Alanine; Amino Acid Sequence; Cloning, Molecular; Escherichia coli; Glutamine; GTP Phosphohydrolase-Linked Elongation Factors; Guanosine Diphosphate; Guanosine Triphosphate; Histidine; Kinetics; Mutagenesis, Site-Directed; Peptide Biosynthesis; Peptide Elongation Factor Tu; Peptides; Point Mutation; Recombinant Proteins

1995
Mechanism of modulation of rat liver fructose-2,6-bisphosphatase by nucleoside triphosphates.
    The Journal of biological chemistry, 1994, Apr-15, Volume: 269, Issue:15

    Topics: Adenosine Triphosphate; Alanine; Amino Acid Sequence; Animals; Arginine; Chromatography, Gel; Electrophoresis, Polyacrylamide Gel; Guanosine Triphosphate; Kinetics; Liver; Lysine; Molecular Weight; Mutagenesis, Site-Directed; Phosphofructokinase-2; Phosphoric Monoester Hydrolases; Point Mutation; Rats; Recombinant Proteins; Sequence Deletion

1994
Activation of the mitogen-activated protein kinase pathway in Triton X-100 disrupted NIH-3T3 cells by p21 ras and in vitro by plasma membranes from NIH 3T3 cells.
    Molecular biology of the cell, 1993, Volume: 4, Issue:5

    Topics: 3T3 Cells; Alanine; Animals; Cell Line, Transformed; Cell Membrane; Cell-Free System; Enzyme Activation; Guanosine Triphosphate; Insulin; Mice; Microscopy, Electron; Mitogen-Activated Protein Kinase 1; Octoxynol; Platelet-Derived Growth Factor; Polyethylene Glycols; Protein Serine-Threonine Kinases; Protein-Tyrosine Kinases; Proto-Oncogene Proteins p21(ras); Signal Transduction

1993
Rapid kinetic measurements of second messenger formation in olfactory cilia from channel catfish.
    The American journal of physiology, 1993, Volume: 264, Issue:4 Pt 1

    Topics: Alanine; Amino Acids; Animals; Cilia; Cyclic AMP; Cyclic GMP; Cysteine; Epithelium; Guanine Nucleotides; Guanosine 5'-O-(3-Thiotriphosphate); Guanosine Diphosphate; Guanosine Triphosphate; Ictaluridae; Inositol 1,4,5-Trisphosphate; Kinetics; Olfactory Mucosa; Second Messenger Systems; Thionucleotides

1993
Investigation of the role of conserved serine residues in the long form of the rat D2 dopamine receptor using site-directed mutagenesis.
    Journal of neurochemistry, 1996, Volume: 66, Issue:1

    Topics: Alanine; Allosteric Regulation; Animals; Cell Line, Transformed; Chlorocebus aethiops; Dopamine Agonists; Dopamine Antagonists; GTP-Binding Proteins; Guanosine Triphosphate; Hydrogen Bonding; Mutagenesis, Site-Directed; Protein Binding; Protein Structure, Tertiary; Rats; Receptors, Dopamine D2; Serine; Spiperone; Structure-Activity Relationship; Transfection

1996
Site-directed mutagenesis of Thermus thermophilus EF-Tu: the substitution of threonine-62 by serine or alanine.
    FEBS letters, 1995, Dec-18, Volume: 377, Issue:2

    Topics: Alanine; Base Sequence; Binding Sites; DNA Primers; GTP Phosphohydrolase-Linked Elongation Factors; Guanosine Diphosphate; Guanosine Triphosphate; Molecular Sequence Data; Mutagenesis, Site-Directed; Peptide Elongation Factor Tu; RNA, Transfer, Amino Acyl; Serine; Thermus thermophilus; Threonine

1995
Uncoupling of GTP binding from target stimulation by a single mutation in the transducin alpha subunit.
    Science (New York, N.Y.), 1996, Mar-08, Volume: 271, Issue:5254

    Topics: 3',5'-Cyclic-GMP Phosphodiesterases; Adenosine Diphosphate Ribose; Alanine; Animals; Base Sequence; Cattle; Enzyme Activation; Glutamic Acid; Guanosine 5'-O-(3-Thiotriphosphate); Guanosine Diphosphate; Guanosine Triphosphate; Molecular Sequence Data; Mutation; Protein Conformation; Protein Structure, Secondary; Protein Structure, Tertiary; Recombinant Fusion Proteins; Rhodopsin; Transducin

1996
The differential effects of the Gly-60 to Ala mutation on the interaction of H-Ras p21 with different downstream targets.
    The Journal of biological chemistry, 1996, Apr-05, Volume: 271, Issue:14

    Topics: Alanine; Amino Acid Sequence; Animals; Base Sequence; DNA Primers; Glycine; GTP Phosphohydrolases; GTPase-Activating Proteins; Guanosine Triphosphate; Macromolecular Substances; Mice; Molecular Sequence Data; Neurofibromin 1; Protein Serine-Threonine Kinases; Proteins; Proto-Oncogene Proteins; Proto-Oncogene Proteins c-raf; Proto-Oncogene Proteins p21(ras); ras GTPase-Activating Proteins; Signal Transduction; Structure-Activity Relationship

1996
Alanine scanning mutagenesis of conserved arginine/lysine-arginine/lysine-X-X-arginine/lysine G protein-activating motifs on m1 muscarinic acetylcholine receptors.
    Molecular pharmacology, 1996, Volume: 50, Issue:1

    Topics: Alanine; Amino Acid Sequence; Animals; Base Sequence; Binding Sites; Binding, Competitive; Carbachol; Cell Membrane; CHO Cells; Conserved Sequence; Cricetinae; DNA Primers; GTP-Binding Proteins; Guanosine Triphosphate; Kinetics; Molecular Sequence Data; Mutagenesis, Site-Directed; N-Methylscopolamine; Oxotremorine; Protein Structure, Secondary; Quinuclidinyl Benzilate; Receptor, Muscarinic M2; Receptors, Muscarinic; Recombinant Proteins; Scopolamine Derivatives; Second Messenger Systems; Transfection

1996
Significance of Thr182 in the nucleotide-exchange and GTP-hydrolysis reactions of the alpha subunit of GTP-binding protein Gi2.
    Journal of biochemistry, 1995, Volume: 118, Issue:5

    Topics: Alanine; Amino Acid Sequence; Animals; Base Sequence; Escherichia coli; GTP-Binding Protein alpha Subunit, Gi2; GTP-Binding Protein alpha Subunits, Gi-Go; GTP-Binding Proteins; Guanosine Triphosphate; Hydrolysis; Magnesium; Molecular Sequence Data; Mutagenesis, Site-Directed; Nucleotides; Peptide Fragments; Proto-Oncogene Proteins; Rats; Recombinant Proteins; Threonine

1995
Structure of the GDP-Pi complex of Gly203-->Ala gialpha1: a mimic of the ternary product complex of galpha-catalyzed GTP hydrolysis.
    Structure (London, England : 1993), 1996, Nov-15, Volume: 4, Issue:11

    Topics: Alanine; Binding Sites; Crystallography, X-Ray; Enzyme Activation; Glycine; GTP Phosphohydrolases; GTP-Binding Protein alpha Subunits, Gi-Go; Guanosine 5'-O-(3-Thiotriphosphate); Guanosine Diphosphate; Guanosine Triphosphate; Magnesium; Models, Molecular; Molecular Mimicry; Molecular Sequence Data; Mutation; Phosphates; Protein Conformation; Protein Structure, Secondary

1996
Characterisation of the interaction between guanyl nucleotides and AMPA receptors in rat brain.
    Neuropharmacology, 1996, Volume: 35, Issue:11

    Topics: Alanine; Animals; Autoradiography; Brain Chemistry; Excitatory Amino Acid Agonists; Female; Guanine Nucleotides; Guanosine Triphosphate; In Vitro Techniques; Membranes; Pyrimidines; Radioligand Assay; Rats; Rats, Wistar; Receptors, AMPA; Thiocyanates

1996
Cloning and characterization of a dominant-negative vps1 allele of the yeast Saccharomyces cerevisiae.
    Biological chemistry, 1997, Volume: 378, Issue:10

    Topics: Alanine; Alleles; Amino Acid Sequence; Animals; Asparagine; Binding Sites; Carrier Proteins; Cloning, Molecular; Gene Expression Regulation, Fungal; Genes, Fungal; GTP-Binding Proteins; Guanosine Triphosphate; Humans; Mice; Molecular Sequence Data; Rats; Saccharomyces cerevisiae; Sequence Homology, Amino Acid; Vesicular Transport Proteins

1997
Identification of amino acid residues of Gsalpha critical to repression of adipogenesis.
    The Journal of biological chemistry, 1998, May-08, Volume: 273, Issue:19

    Topics: 3T3 Cells; Adipocytes; Alanine; Amino Acid Sequence; Animals; Cell Differentiation; Cyclic AMP; GTP-Binding Protein alpha Subunits, Gs; Guanosine Triphosphate; Mice; Models, Molecular; Molecular Sequence Data; Mutagenesis, Site-Directed; Protein Structure, Tertiary; Recombinant Fusion Proteins; Sequence Alignment; Structure-Activity Relationship

1998
Mutational analysis of mammalian translation initiation factor 5 (eIF5): role of interaction between the beta subunit of eIF2 and eIF5 in eIF5 function in vitro and in vivo.
    Molecular and cellular biology, 2000, Volume: 20, Issue:11

    Topics: Alanine; Amino Acid Substitution; Animals; Artemia; Eukaryotic Initiation Factor-2; Eukaryotic Initiation Factor-3; Eukaryotic Initiation Factor-5; Fungal Proteins; Guanosine Triphosphate; Hydrolysis; Mammals; Mutagenesis; Nuclear Proteins; Peptide Initiation Factors; Protein Biosynthesis; Rabbits; Rats; Recombinant Fusion Proteins; Ribosomal Proteins; Saccharomyces cerevisiae; Saccharomyces cerevisiae Proteins

2000
Modeling ischemia in vitro: selective depletion of adenine and guanine nucleotide pools.
    American journal of physiology. Cell physiology, 2000, Volume: 279, Issue:4

    Topics: Adenosine Triphosphate; Alanine; Animals; Antibiotics, Antineoplastic; Antimycin A; Apoptosis; Cell Hypoxia; Cells, Cultured; Deoxyglucose; Enzyme Inhibitors; Guanosine; Guanosine Triphosphate; Ischemia; Kidney Cortex; Kidney Tubules, Proximal; LLC-PK1 Cells; Male; Models, Biological; Mycophenolic Acid; Necrosis; Oxidative Phosphorylation; Rats; Rats, Sprague-Dawley; Swine

2000
Three-dimensional view of the surface motif associated with the P-loop structure: cis and trans cases of convergent evolution.
    Journal of molecular biology, 2000, Nov-03, Volume: 303, Issue:4

    Topics: Adenosine Triphosphate; Alanine; Amino Acid Motifs; Amino Acid Sequence; Animals; Arginine; Asparagine; Binding Sites; Conserved Sequence; Evolution, Molecular; GTP-Binding Proteins; Guanosine Triphosphate; Humans; Models, Molecular; Molecular Sequence Data; Nucleoside-Phosphate Kinase; Protein Structure, Tertiary; Ribonucleases; Sequence Alignment; Static Electricity; Substrate Specificity

2000
A post-translational modification in the GGQ motif of RF2 from Escherichia coli stimulates termination of translation.
    The EMBO journal, 2000, Dec-15, Volume: 19, Issue:24

    Topics: Alanine; Amino Acid Sequence; Amino Acid Substitution; Escherichia coli; Guanosine Diphosphate; Guanosine Triphosphate; Kinetics; Peptide Chain Termination, Translational; Peptide Fragments; Peptide Termination Factors; Protein Processing, Post-Translational; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Threonine

2000
Alanine scan mutagenesis of the switch I domain of the Caulobacter crescentus CgtA protein reveals critical amino acids required for in vivo function.
    Molecular microbiology, 2001, Volume: 39, Issue:4

    Topics: Alanine; Amino Acid Sequence; Amino Acid Substitution; Bacterial Proteins; Binding Sites; Caulobacter crescentus; Guanine; Guanosine Diphosphate; Guanosine Triphosphate; Hydrolysis; Molecular Sequence Data; Monomeric GTP-Binding Proteins; Mutagenesis, Site-Directed; Phosphorylation; Promoter Regions, Genetic; Xylose

2001
Identification of thermodynamically relevant interactions between EF-Tu and backbone elements of tRNA.
    Journal of molecular biology, 2001, May-18, Volume: 308, Issue:5

    Topics: Alanine; Base Sequence; Binding Sites; Guanosine Triphosphate; Hydrogen Bonding; Models, Molecular; Molecular Sequence Data; Mutation; Nuclease Protection Assays; Nucleic Acid Conformation; Peptide Elongation Factor Tu; Phenylalanine; Protein Binding; Protein Conformation; Protons; RNA, Bacterial; RNA, Transfer; RNA, Transfer, Ala; RNA, Transfer, Phe; Thermodynamics; Thermus; Thermus thermophilus

2001
Mutations in the G-domain of elongation factor G from Thermus thermophilus affect both its interaction with GTP and fusidic acid.
    The Journal of biological chemistry, 2001, Aug-03, Volume: 276, Issue:31

    Topics: Alanine; Aspartic Acid; Binding Sites; Cell-Free System; Fusidic Acid; Genetic Variation; Guanosine Diphosphate; Guanosine Triphosphate; Guanylyl Imidodiphosphate; Kinetics; Lysine; Models, Molecular; Mutagenesis, Site-Directed; Peptide Elongation Factor G; Poly U; Protein Biosynthesis; Protein Structure, Tertiary; Recombinant Proteins; Thermus thermophilus; Threonine

2001
Simultaneous and functional binding of SmpB and EF-Tu-TP to the alanyl acceptor arm of tmRNA.
    Journal of molecular biology, 2001, Nov-16, Volume: 314, Issue:1

    Topics: Acylation; Alanine; Alanine-tRNA Ligase; Base Sequence; Binding Sites; Electrophoretic Mobility Shift Assay; Escherichia coli; Escherichia coli Proteins; Guanosine Triphosphate; Kinetics; Molecular Sequence Data; Nuclease Protection Assays; Nucleic Acid Conformation; Peptide Elongation Factor Tu; Protein Binding; Ribonucleases; Ribosomal Proteins; RNA-Binding Proteins; RNA, Bacterial; RNA, Transfer, Amino Acyl

2001
Role of T7 RNA polymerase His784 in start site selection and initial transcription.
    Biochemistry, 2002, Apr-23, Volume: 41, Issue:16

    Topics: Alanine; Bacteriophage T7; Base Sequence; Binding Sites; DNA-Directed RNA Polymerases; Glutamine; Guanosine; Guanosine Triphosphate; Histidine; Hydrogen Bonding; Mutagenesis, Site-Directed; Promoter Regions, Genetic; Stereoisomerism; Transcription Initiation Site; Viral Proteins

2002
Aspartate-107 and leucine-109 facilitate efficient coupling of glutamine hydrolysis to CTP synthesis by Escherichia coli CTP synthase.
    The Biochemical journal, 2003, Feb-01, Volume: 369, Issue:Pt 3

    Topics: Alanine; Amino Acid Sequence; Aspartic Acid; Carbon-Nitrogen Ligases; Circular Dichroism; Conserved Sequence; Cytidine Triphosphate; Dimerization; Escherichia coli Proteins; Glutamine; Guanosine Triphosphate; Hydrolysis; Leucine; Molecular Sequence Data; Mutagenesis, Site-Directed

2003
Visualizing tmRNA entry into a stalled ribosome.
    Science (New York, N.Y.), 2003, Apr-04, Volume: 300, Issue:5616

    Topics: Alanine; Anticodon; Bacterial Proteins; Codon; Codon, Terminator; Cryoelectron Microscopy; Guanosine Triphosphate; Image Processing, Computer-Assisted; Models, Molecular; Nucleic Acid Conformation; Open Reading Frames; Peptide Elongation Factor Tu; Protein Biosynthesis; Pyridones; Ribosomal Proteins; Ribosomes; RNA-Binding Proteins; RNA, Bacterial; RNA, Messenger; RNA, Transfer; Thermus thermophilus

2003
Phosphorylation of threonine 10 on CKBBP1/SAG/ROC2/Rbx2 by protein kinase CKII promotes the degradation of IkappaBalpha and p27Kip1.
    The Journal of biological chemistry, 2003, Aug-01, Volume: 278, Issue:31

    Topics: Alanine; Casein Kinase II; Cell Cycle Proteins; Cell Division; Cyclin-Dependent Kinase Inhibitor p27; Cysteine Endopeptidases; Enzyme Inhibitors; G1 Phase; Gene Expression; Guanosine Triphosphate; HeLa Cells; Humans; I-kappa B Proteins; Leupeptins; Multienzyme Complexes; Mutagenesis, Site-Directed; NF-KappaB Inhibitor alpha; Phosphorylation; Proteasome Endopeptidase Complex; Protein Serine-Threonine Kinases; RNA; RNA-Binding Proteins; S Phase; Structure-Activity Relationship; Threonine; Transfection; Tumor Suppressor Proteins; Ubiquitin-Protein Ligases

2003
Mutation of cysteine 214 in Gi1 alpha subunit abolishes its endogenous GTPase activity.
    The Biochemical journal, 2004, May-01, Volume: 379, Issue:Pt 3

    Topics: Adenosine Diphosphate; Adenylyl Cyclases; Alanine; Amino Acid Substitution; Animals; CHO Cells; Colforsin; Cricetinae; Cyclic AMP; Cysteine; GTP-Binding Protein alpha Subunits, Gi-Go; Guanosine Triphosphate; Hydrolysis; Kinetics; Mutation; Pertussis Toxin; Protein Binding; Protein Subunits; RGS Proteins; Thermodynamics; Trypsin; Tryptophan

2004
A novel 3-step enantioselective synthesis of pyrenylalanine with subsequent incorporation into opioid, CCK, and melanotropin ligands.
    Biochemical and biophysical research communications, 2004, May-28, Volume: 318, Issue:2

    Topics: Alanine; Amino Acid Sequence; Cholecystokinin; Cyclic AMP; Enkephalin, D-Penicillamine (2,5)-; Guanosine Triphosphate; Humans; Ligands; Melanocyte-Stimulating Hormones; Molecular Structure; Narcotics; Oligopeptides; Protein Binding; Pyrenes; Receptors, Melanocortin; Receptors, Opioid, delta; Stereoisomerism

2004
Dynamin GTPase domain mutants that differentially affect GTP binding, GTP hydrolysis, and clathrin-mediated endocytosis.
    The Journal of biological chemistry, 2004, Sep-24, Volume: 279, Issue:39

    Topics: Adenoviridae; Alanine; Baculoviridae; Binding Sites; Chromatography, Gel; Clathrin; Dynamins; Endocytosis; GTP Phosphohydrolases; Guanosine Triphosphate; Humans; Hydrolysis; Kinetics; Lipids; Models, Chemical; Mutation; Oligonucleotides; Point Mutation; Protein Binding; Protein Conformation; Protein Structure, Tertiary; Serine; Temperature; Threonine; Time Factors; Transferrin

2004
Interactions of eukaryotic translation initiation factor 3 (eIF3) subunit NIP1/c with eIF1 and eIF5 promote preinitiation complex assembly and regulate start codon selection.
    Molecular and cellular biology, 2004, Volume: 24, Issue:21

    Topics: Alanine; Amino Acid Sequence; Codon, Initiator; DNA-Binding Proteins; Eukaryotic Initiation Factor-1; Eukaryotic Initiation Factor-3; Eukaryotic Initiation Factor-5; Gene Expression Regulation, Fungal; Guanosine Triphosphate; Models, Molecular; Molecular Sequence Data; Mutagenesis, Site-Directed; Nuclear Proteins; Phenotype; Prokaryotic Initiation Factor-3; Protein Binding; Protein Biosynthesis; Protein Kinases; Protein Structure, Quaternary; Protein Subunits; Ribosomes; RNA, Transfer, Met; Saccharomyces cerevisiae; Saccharomyces cerevisiae Proteins

2004
Structure of the G60A mutant of Ras: implications for the dominant negative effect.
    The Journal of biological chemistry, 2005, Jul-08, Volume: 280, Issue:27

    Topics: Alanine; Amino Acid Substitution; Animals; Chlorocebus aethiops; COS Cells; Crystallography; Glycine; Guanosine Diphosphate; Guanosine Triphosphate; Mice; NIH 3T3 Cells; Oncogene Protein p21(ras); Oocytes; Protein Binding; Protein Structure, Secondary; Protein Structure, Tertiary; Xenopus

2005
Mutations of Cys-17 and Ala-271 in the human histamine H2 receptor determine the species selectivity of guanidine-type agonists and increase constitutive activity.
    The Journal of pharmacology and experimental therapeutics, 2007, Volume: 321, Issue:3

    Topics: Adenylyl Cyclases; Alanine; Animals; Baculoviridae; Cell Line; Cell Membrane; Cysteine; GTP Phosphohydrolases; GTP-Binding Protein alpha Subunits, Gs; Guanidine; Guanosine Triphosphate; Guinea Pigs; Histamine; Histamine Agonists; Histamine H2 Antagonists; Humans; Mutation, Missense; Receptors, Histamine H2; Recombinant Fusion Proteins; Species Specificity; Spodoptera; Transfection

2007
The site for the allosteric activator GTP of Escherichia coli UMP kinase.
    FEBS letters, 2009, Jan-05, Volume: 583, Issue:1

    Topics: Alanine; Allosteric Regulation; Amino Acid Substitution; Arginine; Binding Sites; Enzyme Activation; Escherichia coli; Guanosine Triphosphate; Guanylyl Imidodiphosphate; Histidine; Mutagenesis; Mutation; Nucleoside-Phosphate Kinase; Protein Conformation; Uridine Triphosphate

2009
A sequence element that tunes Escherichia coli tRNA(Ala)(GGC) to ensure accurate decoding.
    Nature structural & molecular biology, 2009, Volume: 16, Issue:4

    Topics: Alanine; Anticodon; Base Sequence; Codon; Conserved Sequence; Escherichia coli; Guanosine Triphosphate; Kinetics; Models, Molecular; Point Mutation; Protein Biosynthesis; RNA, Transfer, Ala

2009
Mutational analysis of the GTP-binding motif of FlhF which regulates the number and placement of the polar flagellum in Vibrio alginolyticus.
    Journal of biochemistry, 2009, Volume: 146, Issue:5

    Topics: Alanine; Amino Acid Motifs; Amino Acid Sequence; Bacterial Proteins; Cell Polarity; DNA Mutational Analysis; Flagella; Green Fluorescent Proteins; Guanosine Triphosphate; Immunoprecipitation; Molecular Sequence Data; Monomeric GTP-Binding Proteins; Movement; Mutant Proteins; Mutation; Plasmids; Protein Binding; Protein Transport; Recombinant Fusion Proteins; Vibrio alginolyticus

2009
Phosphorylation of CalDAG-GEFI by protein kinase A prevents Rap1b activation.
    Journal of thrombosis and haemostasis : JTH, 2013, Volume: 11, Issue:8

    Topics: Alanine; Amino Acid Sequence; Blood Platelets; Calcium Signaling; Cyclic AMP; Cyclic AMP-Dependent Protein Kinases; Enzyme Activation; Guanine Nucleotide Exchange Factors; Guanosine Triphosphate; HEK293 Cells; Humans; Immunoprecipitation; Mass Spectrometry; Molecular Sequence Data; Mutation; Phosphorylation; Plasmids; Platelet Aggregation; rab1 GTP-Binding Proteins; Sequence Homology, Amino Acid; Signal Transduction

2013
Rapamycin enhances eIF4E phosphorylation by activating MAP kinase-interacting kinase 2a (Mnk2a).
    FEBS letters, 2013, Aug-19, Volume: 587, Issue:16

    Topics: Alanine; Amino Acid Sequence; Animals; Cell Line, Tumor; Enzyme Activation; Eukaryotic Initiation Factor-4E; Fibroblasts; Gene Expression Regulation, Enzymologic; Guanosine Triphosphate; Humans; Intracellular Signaling Peptides and Proteins; MAP Kinase Signaling System; Mice; Molecular Sequence Data; Mutation; Phosphorylation; Protein Serine-Threonine Kinases; Protein Structure, Tertiary; Serine; Sirolimus

2013
Structure-guided mutation of the conserved G3-box glycine in Rheb generates a constitutively activated regulator of mammalian target of rapamycin (mTOR).
    The Journal of biological chemistry, 2014, May-02, Volume: 289, Issue:18

    Topics: Alanine; Amino Acid Motifs; Animals; Binding Sites; Cells, Cultured; Crystallography, X-Ray; Glycine; Guanosine Triphosphate; HeLa Cells; Humans; Hydrolysis; Mechanistic Target of Rapamycin Complex 1; Mice; Models, Molecular; Monomeric GTP-Binding Proteins; Multiprotein Complexes; Mutation; Neuropeptides; Phosphorylation; Protein Binding; Protein Structure, Tertiary; Ras Homolog Enriched in Brain Protein; Ribosomal Protein S6 Kinases, 70-kDa; Signal Transduction; TOR Serine-Threonine Kinases

2014
Anti-HCV, nucleotide inhibitors, repurposing against COVID-19.
    Life sciences, 2020, May-01, Volume: 248

    Topics: Adenosine Monophosphate; Alanine; Alphacoronavirus; Amino Acid Sequence; Antiviral Agents; Betacoronavirus; Catalytic Domain; Computational Biology; Coronavirus Infections; COVID-19; COVID-19 Drug Treatment; Drug Repositioning; Guanosine Monophosphate; Guanosine Triphosphate; Humans; Molecular Docking Simulation; Pneumonia, Viral; Protein Binding; Protein Conformation, alpha-Helical; Protein Conformation, beta-Strand; Protein Interaction Domains and Motifs; Ribavirin; RNA-Dependent RNA Polymerase; SARS-CoV-2; Sequence Alignment; Sequence Homology, Amino Acid; Sofosbuvir; Thermodynamics; Uridine Triphosphate; Viral Proteins

2020