alanine and azidohomoalanine

alanine has been researched along with azidohomoalanine in 71 studies

Research

Studies (71)

TimeframeStudies, this research(%)All Research%
pre-19900 (0.00)18.7374
1990's0 (0.00)18.2507
2000's9 (12.68)29.6817
2010's54 (76.06)24.3611
2020's8 (11.27)2.80

Authors

AuthorsStudies
Link, AJ; Tirrell, DA1
Back, JW; David, O; de Jong, L; de Koning, LJ; de Koster, CG; Kasper, PT; Kramer, G; Masson, G; van Maarseveen, JH1
Dieterich, DC; Graumann, J; Lee, JJ; Link, AJ; Schuman, EM; Tirrell, DA1
Link, AJ; Tirrell, DA; Vink, MK2
Grabstein, K; Johnson, RS; Tirrell, DA; Wang, A; Winblade Nairn, N1
Morimoto, J; Murakami, H; Sako, Y; Suga, H1
Back, J; de Jong, L; de Koning, LJ; de Koster, CG; Dekker, HL; Hellingwerf, KJ; Kramer, G; Nessen, MA; Sprenger, RR; van Maarseveen, JH1
Back, J; Baskin, JM; Bertozzi, CR; de Jong, L; de Koning, LJ; de Koster, CG; Hiemstra, H; Kramer, G; Nessen, MA; Smeenk, LE; van Maarseveen, JH1
Deal, RB; Henikoff, JG; Henikoff, S1
Dieterich, DC; Gouzer, G; Hodas, JJ; Ngo, JT; Schuman, EM; Shadrin, IY; Tirrell, DA; Triller, A1
Deal, RB; Henikoff, S1
Back, J; de Jong, L; de Koster, CG; de Mattos, MJ; Kramer, G; Nessen, MA; Roseboom, W; Speijer, D; Sprenger, RR1
Banerjee, PS; Carrico, I; Chung, J; Dyer, RB; Nagarajan, S; Raleigh, DP; Taskent-Sezgin, H1
Burnham, DR; Chiu, DT; Jin, Y; Schneider, T; Smith, PB; Wu, C1
Drewe, WC; Roth, S; Thomas, NR1
Garner, AL; Janda, KD; Kaufmann, GF; Kim, SK; Kravchenko, VV; Lowery, CA; Mayorov, AV; Park, J; Struss, AK; Yu, J; Zhu, J1
David, L; Simon, RP; Zhou, A1
Patel, KG; Swartz, JR1
de Jong, L; de Koster, CG; Kasper, PT; Kramer, G1
Banerjee, PS; Carrico, IS1
Plückthun, A; Simon, M; Zangemeister-Wittke, U1
Choi, KY; Ezzatti, P; Lippert, DN; Mookherjee, N1
Budisa, N; Lee, SG; Paik, HJ; Raghunathan, G; Sokalingam, S; Soundrarajan, N; Yoo, TH1
Bloem, R; Buchli, B; Hamm, P; Jelesarov, I; Koziol, K; Samatanga, B; Waldauer, SA; Walser, R1
Agris, PF; Fanning, KD; Francis, SP; Harris, KA; Katz, J; Lacy, M; Nicholas, BD; Pagana, J; Shin, JB1
Eichelbaum, K; Krijgsveld, J1
Bredenbeck, J; Müller-Werkmeister, HM1
Biava, H; Budisa, N; Contestabile, R; di Salvo, ML; Ma, Y1
Banister, S; Chew, YL; Götz, J; Kassiou, M; Lam, H; Liang, V; Nicholas, HR; Song, X; Ullrich, M; Zaw, T1
Bian, F; David, L; Frankel, M; Hall, CL; Li, Y; Simon, RP; Wainwright, J; Zhou, A1
Babin, BM; Hatzenpichler, R; Orphan, VJ; Scheller, S; Tavormina, PL; Tirrell, DA1
Lin, Q; Ng, S; Shen, HM; Wang, J; Zhang, J1
Banister, S; Berber, S; Chew, YL; Götz, J; Kassiou, M; Lam, H; Liang, V; Nicholas, HR; Song, X; Ullrich, M; Zaw, T1
Ballikaya, S; Gebert, J; Kopitz, J; Lee, J; Schnölzer, M; Warnken, U1
Bowling, H; Chao, MV; Hom, N; Kirshenbaum, K; Klann, E; Neubert, TA; Zhang, G1
Antileo, ER; Dieterich, DC; Landgraf, P; Schuman, EM1
Chang, YC; Chen, EC; Chung, HW; Fang, W; Hsu, WL; Lee, YT; Wu, CY; Wu, HI1
Hellberg, K; Liu, C; Ma, Y; Martínez-Bartolomé, S; McClatchy, DB; Shaw, RJ; Stein, BD; Vasquez, D; Yates, JR1
Budisa, N; Haag, R; Ma, Y; Thota, BN1
Dieterich, DC; Freitag, CE; Landgraf, P; Müller, A; Stellmacher, A1
Bhuvanagiri, M; Hauer, C; Hentze, MW; Krijgsveld, J; Kulozik, AE; Leicht, S; Neu-Yilik, G; Sieber, J1
Brynildsen, MP; Henry, TC1
Aschrafi, A; Gioio, A; Kaplan, BB; Kos, A; Martens, GJ; Wanke, KA1
Bao, F; Koh, PL; Lee, YM; Lin, Q; Ng, S; Shen, HM; Wang, J; Zhang, J1
Chai, Q; Dutch, RE; Wang, Z; Webb, SR; Wei, Y1
Calve, S; Kinzer-Ursem, TL; Ocken, AR; Witten, AJ1
Daub, E; Honek, JF; Urquhart, T1
Chen, W; Donnelly, N; Hou, J; Marsh, JA; McShane, E; Selbach, M; Sin, C; Storchova, Z; Valleriani, A; Wang, X; Wells, JN; Zauber, H1
Chen, X; Li, Z; Liu, W; Qin, K; Sun, Y; Zhou, W; Zhu, Y1
Sheng, L; Wang, J; Zhang, S; Zhang, X; Zhao, H1
Mauck, RL; McLeod, CM1
Hua, ZC; Lee, YM; Lin, Q; Ng, S; Shen, HM; Shi, Y; Wang, J; Zhang, J1
Bailey-Serres, J; Glenn, WS; Hess, S; Ho, SH; Moradian, A; Stone, SE; Sweredoski, MJ; Tirrell, DA1
Lee, YM; Lim, TK; Lin, Q; Shen, HM; Wang, J; Zhang, J1
Itzhaki, LS; Ley, SV; Serrano, JC; Sipthorp, J; Xu, W1
Barkallah, S; Ma, Y; McClatchy, DB; Wood, WW; Yates, JR1
Bredenbeck, J; Eberl, KB; Kudlinzki, D; Lehner, F; Müller-Werkmeister, HM; Richter, C; Schwalbe, H; Silvers, R1
Huang, LL; Liu, K; Xie, HY; Xu, J; Zhang, Q; Zhao, D; Zhu, H1
Budisa, N; Di Salvo, ML; Ma, Y1
Ma, Y; Yates, JR1
Bernhard, E; Bethi, M; Borra, V; Murakami, K; Nakamura, T; Salem, ESB; Takahashi, T1
Brandl, F; Jones, AT; Lorenzer, C; Merten, H; Plückthun, A; Sayers, EJ; Streußnig, S; Tot, E; Watson, P; Winkler, AM; Winkler, J; Zangemeister-Wittke, U1
Gao, Y; Lavallée-Adam, M; Martínez-Bartolomé, S; McClatchy, DB; Yates, JR1
Ghazanfar, S; Han, X; Hou, Z; Liu, H; Wang, Z; Yang, J1
Meuwly, M; Salehi, SM1
Duncan, O; Fenske, R; Millar, AH; Tivendale, ND1
Jeon, T; Kang, C; Kim, J; Kim, MS; Lee, Y; Roh, K1
Cai, Z; Gygi, SP; Harry, BL; Huttlin, EL; Li, J; Mitchell, DC; Paulo, JA; Shen, N; Vaites, LP1
Arnold-Schild, D; Bopp, T; Chen, Y; Distler, U; Kirschner, F; Klein, M; Kücük, C; Leps, C; Ludt, A; Marini, F; Michna, T; Rajalingam, K; Riegel, K; Schild, H; Sielaff, M; Stein, L; Tenzer, S; Łącki, MK1
Jecmen, T; Sulc, M; Tuzhilkin, R1

Reviews

1 review(s) available for alanine and azidohomoalanine

ArticleYear
Proteomics and pulse azidohomoalanine labeling of newly synthesized proteins: what are the potential applications?
    Expert review of proteomics, 2018, Volume: 15, Issue:7

    Topics: Alanine; Animals; Humans; Immunity; Isotope Labeling; Microbiota; Protein Biosynthesis; Proteomics; Proteostasis

2018

Trials

1 trial(s) available for alanine and azidohomoalanine

ArticleYear
Nascent proteomes in peripheral blood mononuclear cells as a novel source for biomarker discovery in human stroke.
    Stroke, 2014, Volume: 45, Issue:4

    Topics: Alanine; Biomarkers; Cells, Cultured; Cluster Analysis; Female; Humans; Leukocytes, Mononuclear; Male; Mass Spectrometry; Pilot Projects; Proteome; Proteomics; Staining and Labeling; Stroke

2014

Other Studies

69 other study(ies) available for alanine and azidohomoalanine

ArticleYear
Cell surface labeling of Escherichia coli via copper(I)-catalyzed [3+2] cycloaddition.
    Journal of the American Chemical Society, 2003, Sep-17, Volume: 125, Issue:37

    Topics: Alanine; Catalysis; Copper; Escherichia coli; Gene Expression; Membrane Proteins; Models, Chemical; Molecular Structure; Mutagenesis, Site-Directed; Mutation; Porins; Staining and Labeling

2003
Mild and chemoselective peptide-bond cleavage of peptides and proteins at azido homoalanine.
    Angewandte Chemie (International ed. in English), 2005, Dec-09, Volume: 44, Issue:48

    Topics: Alanine; Amino Acid Sequence; Azides; Molecular Sequence Data; Molecular Structure; Peptides; Phosphines; Proteins

2005
Labeling, detection and identification of newly synthesized proteomes with bioorthogonal non-canonical amino-acid tagging.
    Nature protocols, 2007, Volume: 2, Issue:3

    Topics: Affinity Labels; Alanine; Amino Acids; Animals; Azides; Gene Expression Profiling; Mice; Proteins; Proteomics

2007
Preparation of the functionalizable methionine surrogate azidohomoalanine via copper-catalyzed diazo transfer.
    Nature protocols, 2007, Volume: 2, Issue:8

    Topics: Alanine; Biochemistry; Butyrates; Catalysis; Chromatography, Ion Exchange; Copper; Crystallization; Furans; Methionine; Sulfonamides

2007
Synthesis of the functionalizable methionine surrogate azidohomoalanine using Boc-homoserine as precursor.
    Nature protocols, 2007, Volume: 2, Issue:8

    Topics: Alanine; Biochemistry; Chromatography, Ion Exchange; Crystallization; Formic Acid Esters; Homoserine

2007
Processing of N-terminal unnatural amino acids in recombinant human interferon-beta in Escherichia coli.
    Chembiochem : a European journal of chemical biology, 2008, Jan-25, Volume: 9, Issue:2

    Topics: Alanine; Amino Acid Sequence; Amino Acid Substitution; Amino Acids; Base Sequence; Binding Sites; Electrophoresis, Polyacrylamide Gel; Escherichia coli; Glutamic Acid; Glycine; Histidine; Humans; Interferon Type I; Molecular Sequence Data; Protein Engineering; Recombinant Proteins; Serine; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Substrate Specificity

2008
Ribosomal synthesis of bicyclic peptides via two orthogonal inter-side-chain reactions.
    Journal of the American Chemical Society, 2008, Jun-11, Volume: 130, Issue:23

    Topics: Alanine; Alkynes; Amino Acid Sequence; Amino Acids; Aminobutyrates; Codon; Cysteine; Glycine; Peptides, Cyclic; Ribosomes; RNA, Messenger; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Templates, Genetic

2008
Identification and quantitation of newly synthesized proteins in Escherichia coli by enrichment of azidohomoalanine-labeled peptides with diagonal chromatography.
    Molecular & cellular proteomics : MCP, 2009, Volume: 8, Issue:7

    Topics: Alanine; Chromatography; Chromatography, Liquid; Escherichia coli; Escherichia coli Proteins; Methionine; Molecular Structure; Peptides; Tandem Mass Spectrometry

2009
Selective enrichment of azide-containing peptides from complex mixtures.
    Journal of proteome research, 2009, Volume: 8, Issue:7

    Topics: Alanine; Amino Acid Sequence; Azides; Cations; Chromatography, Ion Exchange; Cross-Linking Reagents; Cytochromes c; Glutarates; Kinetics; Mass Spectrometry; Methionine; Molecular Sequence Data; Peptides; Proteome; Proteomics

2009
Genome-wide kinetics of nucleosome turnover determined by metabolic labeling of histones.
    Science (New York, N.Y.), 2010, May-28, Volume: 328, Issue:5982

    Topics: Alanine; Animals; Cell Line; Drosophila melanogaster; Drosophila Proteins; Genome, Insect; Histones; Kinetics; Methionine; Molecular Probe Techniques; Nucleosomes; Oligonucleotide Array Sequence Analysis

2010
In situ visualization and dynamics of newly synthesized proteins in rat hippocampal neurons.
    Nature neuroscience, 2010, Volume: 13, Issue:7

    Topics: Alanine; Alkynes; Amino Acids; Animals; Cells, Cultured; Fluorescent Dyes; Glycine; Hippocampus; Immunohistochemistry; Microdialysis; Nerve Tissue Proteins; Neurons; Protein Biosynthesis; Proteomics; Quantum Dots; Rats; Rats, Sprague-Dawley; Staining and Labeling

2010
Catching a glimpse of nucleosome dynamics.
    Cell cycle (Georgetown, Tex.), 2010, Sep-01, Volume: 9, Issue:17

    Topics: Alanine; Animals; DNA; Drosophila; Histones; Nucleosomes; Polycomb-Group Proteins; Repressor Proteins

2010
Proteome-wide alterations in Escherichia coli translation rates upon anaerobiosis.
    Molecular & cellular proteomics : MCP, 2010, Volume: 9, Issue:11

    Topics: Alanine; Anaerobiosis; Escherichia coli; Escherichia coli Proteins; Gene Expression Regulation, Bacterial; Protein Biosynthesis; Proteome

2010
Azidohomoalanine: a conformationally sensitive IR probe of protein folding, protein structure, and electrostatics.
    Angewandte Chemie (International ed. in English), 2010, Oct-04, Volume: 49, Issue:41

    Topics: Alanine; Models, Molecular; Protein Conformation; Protein Folding; Protein Structure, Tertiary; Proteins; Ribosomal Proteins; Spectrophotometry, Infrared; Static Electricity

2010
Ultrabright and bioorthogonal labeling of cellular targets using semiconducting polymer dots and click chemistry.
    Angewandte Chemie (International ed. in English), 2010, Dec-03, Volume: 49, Issue:49

    Topics: 3T3 Cells; Alanine; Animals; Click Chemistry; Fluorenes; Fluorescent Dyes; Humans; Mice; Molecular Structure; Particle Size; Polymers; Proteins; Semiconductors; Staining and Labeling; Surface Properties; Tumor Cells, Cultured

2010
A concise and scalable route to L-azidohomoalanine.
    Nature protocols, 2010, Volume: 5, Issue:12

    Topics: Alanine; Aspartic Acid; Molecular Structure; Proteins

2010
Synthesis of 'clickable' acylhomoserine lactone quorum sensing probes: unanticipated effects on mammalian cell activation.
    Bioorganic & medicinal chemistry letters, 2011, May-01, Volume: 21, Issue:9

    Topics: Alanine; Animals; Cells; Click Chemistry; Homoserine; Humans; Lactones; Molecular Structure; Pseudomonas; Quorum Sensing

2011
Nascent proteomes of ischemic-injured and ischemic-tolerant neuronal cells.
    International journal of computational biology and drug design, 2011, Volume: 4, Issue:1

    Topics: Alanine; Animals; Brain; Brain Ischemia; Cells, Cultured; Click Chemistry; Down-Regulation; Gene Expression Profiling; Mice; Neurons; Proteome; Proteomics; Up-Regulation

2011
Surface functionalization of virus-like particles by direct conjugation using azide-alkyne click chemistry.
    Bioconjugate chemistry, 2011, Mar-16, Volume: 22, Issue:3

    Topics: Alanine; Alkynes; Animals; Antigens; Cell Line; Cell-Free System; Click Chemistry; CpG Islands; DNA; Glycine; Granulocyte-Macrophage Colony-Stimulating Factor; Immunoglobulin Idiotypes; Lymphoma, B-Cell; Mice; Polyethylene Glycols; Substrate Specificity; Surface Properties; Vaccines, Virus-Like Particle

2011
Quantitation of newly synthesized proteins by pulse labeling with azidohomoalanine.
    Methods in molecular biology (Clifton, N.J.), 2011, Volume: 753

    Topics: Alanine; Chromatography; Chromatography, Liquid; Escherichia coli; Humans; Molecular Structure; Protein Biosynthesis; Proteins; Proteomics; Software; Staining and Labeling; Tandem Mass Spectrometry

2011
Chemoselective modification of viral proteins bearing metabolically introduced "clickable" amino acids and sugars.
    Methods in molecular biology (Clifton, N.J.), 2011, Volume: 751

    Topics: Adenoviridae; Alanine; Alkynes; Amino Acids; Animals; Azides; Carbohydrate Metabolism; Carbohydrates; Catalysis; Click Chemistry; Copper; Electrophoresis, Polyacrylamide Gel; Rhodamines; Staining and Labeling; Substrate Specificity; Viral Proteins

2011
Facile double-functionalization of designed ankyrin repeat proteins using click and thiol chemistries.
    Bioconjugate chemistry, 2012, Feb-15, Volume: 23, Issue:2

    Topics: Alanine; Ankyrin Repeat; Cell Adhesion Molecules; Click Chemistry; Epithelial Cells; Fluorescent Dyes; Humans; Hydrolysis; Kinetics; Models, Molecular; Molecular Structure; Polyethylene Glycols; Proteins; Sulfhydryl Compounds; Tumor Cells, Cultured

2012
Defining TNF-α and IL-1β induced nascent proteins: combining bio-orthogonal non-canonical amino acid tagging and proteomics.
    Journal of immunological methods, 2012, Aug-31, Volume: 382, Issue:1-2

    Topics: Alanine; Cell Line; Humans; Interleukin-1beta; Mass Spectrometry; Membrane Transport Proteins; Monocytes; Proteome; Proteomics; Tumor Necrosis Factor-alpha

2012
Conjugation of proteins by installing BIO-orthogonally reactive groups at their N-termini.
    PloS one, 2012, Volume: 7, Issue:10

    Topics: Alanine; Alkynes; Click Chemistry; Glycine; Green Fluorescent Proteins; Methionine; Proteins

2012
Ligand binding studied by 2D IR spectroscopy using the azidohomoalanine label.
    The journal of physical chemistry. B, 2012, Nov-26, Volume: 116, Issue:46

    Topics: Alanine; Ligands; Models, Molecular; Spectrophotometry, Infrared; Staining and Labeling

2012
A novel role of cytosolic protein synthesis inhibition in aminoglycoside ototoxicity.
    The Journal of neuroscience : the official journal of the Society for Neuroscience, 2013, Feb-13, Volume: 33, Issue:7

    Topics: Alanine; Alkynes; Aminoglycosides; Animals; Anti-Bacterial Agents; Apoptosis; Blotting, Western; Cell Count; Chick Embryo; Cytosol; Ear Diseases; Enzyme Activation; Evoked Potentials, Auditory, Brain Stem; Glycine; Hair Cells, Auditory; Immunohistochemistry; JNK Mitogen-Activated Protein Kinases; Mice; Mice, Inbred CBA; Niacinamide; Organ Culture Techniques; Phenylurea Compounds; Protein Kinase Inhibitors; Protein Synthesis Inhibitors; RNA, Ribosomal; Sorafenib

2013
Rapid temporal dynamics of transcription, protein synthesis, and secretion during macrophage activation.
    Molecular & cellular proteomics : MCP, 2014, Volume: 13, Issue:3

    Topics: Alanine; Animals; Cell Line; Cell Membrane; Databases, Protein; Gene Expression Regulation; Isotope Labeling; Lipopolysaccharides; Macrophage Activation; Macrophages; Mice; Protein Biosynthesis; Proteolysis; Proteome; Proteomics; RNA, Messenger; Signal Transduction; Time Factors; Transcription Factors; Transcription, Genetic; Transcriptome

2014
A donor-acceptor pair for the real time study of vibrational energy transfer in proteins.
    Physical chemistry chemical physics : PCCP, 2014, Feb-21, Volume: 16, Issue:7

    Topics: Absorption; Alanine; Azulenes; Energy Transfer; Oligopeptides; Proteins; Vibration

2014
Coupling bioorthogonal chemistries with artificial metabolism: intracellular biosynthesis of azidohomoalanine and its incorporation into recombinant proteins.
    Molecules (Basel, Switzerland), 2014, Jan-15, Volume: 19, Issue:1

    Topics: Alanine; Bacterial Proteins; Click Chemistry; Escherichia coli; Fermentation; Genetic Engineering; Metabolic Networks and Pathways; Protein Processing, Post-Translational; Recombinant Proteins; Spectrometry, Mass, Electrospray Ionization

2014
Altered proteostasis in aging and heat shock response in C. elegans revealed by analysis of the global and de novo synthesized proteome.
    Cellular and molecular life sciences : CMLS, 2014, Volume: 71, Issue:17

    Topics: Aging; Alanine; Animals; Blotting, Western; Caenorhabditis elegans; Caenorhabditis elegans Proteins; Click Chemistry; Forecasting; Gene Expression Regulation; Genes, Helminth; Heat-Shock Response; Hot Temperature; Mass Spectrometry; Microscopy, Fluorescence; Protein Folding; Proteome; Proteostasis Deficiencies

2014
In situ visualization of newly synthesized proteins in environmental microbes using amino acid tagging and click chemistry.
    Environmental microbiology, 2014, Volume: 16, Issue:8

    Topics: Alanine; Alkynes; Archaea; Archaeal Proteins; Azides; Bacteria; Bacterial Proteins; Biofilms; Click Chemistry; Fresh Water; Geologic Sediments; Humans; In Situ Hybridization, Fluorescence; Methionine; Microscopy, Fluorescence; Mouth; Staining and Labeling

2014
Development of a novel method for quantification of autophagic protein degradation by AHA labeling.
    Autophagy, 2014, Volume: 10, Issue:5

    Topics: Alanine; Animals; Autophagy; Biological Assay; Cell Survival; Cells, Cultured; Flow Cytometry; Fluorescent Dyes; Hep G2 Cells; Humans; Mice; Proteolysis; Staining and Labeling

2014
Bio-orthogonal labeling as a tool to visualize and identify newly synthesized proteins in Caenorhabditis elegans.
    Nature protocols, 2014, Volume: 9, Issue:9

    Topics: Alanine; Amino Acids; Animals; Blotting, Western; Caenorhabditis elegans Proteins; Chemistry Techniques, Analytical; Click Chemistry; Fluorescent Antibody Technique; Mass Spectrometry; Staining and Labeling

2014
De Novo proteome analysis of genetically modified tumor cells by a metabolic labeling/azide-alkyne cycloaddition approach.
    Molecular & cellular proteomics : MCP, 2014, Volume: 13, Issue:12

    Topics: Activin Receptors, Type II; Alanine; Alkynes; Apoptosis; Apoptosis Regulatory Proteins; Azides; Biotin; Click Chemistry; Cycloaddition Reaction; Doxycycline; Gene Expression Profiling; Gene Expression Regulation, Neoplastic; Genetic Complementation Test; HCT116 Cells; Humans; Molecular Sequence Annotation; Proteome; Recombinases; Signal Transduction; Staining and Labeling; Streptavidin

2014
In-depth quantitative proteomic analysis of de novo protein synthesis induced by brain-derived neurotrophic factor.
    Journal of proteome research, 2014, Dec-05, Volume: 13, Issue:12

    Topics: Alanine; Brain-Derived Neurotrophic Factor; Chromatography, Liquid; HEK293 Cells; Humans; Mass Spectrometry; Protein Biosynthesis; Proteome; Proteomics; Reproducibility of Results

2014
BONCAT: metabolic labeling, click chemistry, and affinity purification of newly synthesized proteomes.
    Methods in molecular biology (Clifton, N.J.), 2015, Volume: 1266

    Topics: Alanine; Chromatography, Affinity; Click Chemistry; Cycloaddition Reaction; HEK293 Cells; Humans; Proteome; Staining and Labeling

2015
Glutamate Stimulates Local Protein Synthesis in the Axons of Rat Cortical Neurons by Activating α-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid (AMPA) Receptors and Metabotropic Glutamate Receptors.
    The Journal of biological chemistry, 2015, Aug-21, Volume: 290, Issue:34

    Topics: Alanine; alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid; Animals; Axons; Brain-Derived Neurotrophic Factor; Calcium Channels; Calcium Signaling; Embryo, Mammalian; Gene Expression Regulation, Developmental; Glutamic Acid; Hippocampus; Kinetics; Primary Cell Culture; Protein Biosynthesis; Rats; Rats, Sprague-Dawley; Receptors, AMPA; Receptors, Metabotropic Glutamate; Synapses; TOR Serine-Threonine Kinases; TRPV Cation Channels

2015
Pulsed Azidohomoalanine Labeling in Mammals (PALM) Detects Changes in Liver-Specific LKB1 Knockout Mice.
    Journal of proteome research, 2015, Nov-06, Volume: 14, Issue:11

    Topics: Alanine; Alkynes; AMP-Activated Protein Kinases; Animals; Azides; Biotin; Click Chemistry; Food, Formulated; Gene Expression; Integrases; Liver; Male; Methionine; Mice; Mice, Inbred C57BL; Mice, Knockout; Molecular Sequence Annotation; Protein Serine-Threonine Kinases; Proteome; Proteomics

2015
Dendronylation: Residue-specific chemoselective attachment of oligoglycerol dendrimers on proteins with noncanonical amino acids.
    Bioorganic & medicinal chemistry letters, 2015, Nov-15, Volume: 25, Issue:22

    Topics: Alanine; Amino Acid Substitution; Azides; Bacillus; Bacterial Proteins; Cell Line; Click Chemistry; Dendrimers; Escherichia coli; Glycerol; Methionine; Recombinant Proteins

2015
Monitoring Astrocytic Proteome Dynamics by Cell Type-Specific Protein Labeling.
    PloS one, 2015, Volume: 10, Issue:12

    Topics: Alanine; Animals; Astrocytes; Azides; Brain-Derived Neurotrophic Factor; Coculture Techniques; Green Fluorescent Proteins; HEK293 Cells; Humans; Methionine-tRNA Ligase; Neuroglia; Norleucine; Protein Biosynthesis; Proteins; Proteome; Proteomics; Rats, Wistar

2015
Proteomic Analysis Reveals Branch-specific Regulation of the Unfolded Protein Response by Nonsense-mediated mRNA Decay.
    Molecular & cellular proteomics : MCP, 2016, Volume: 15, Issue:5

    Topics: Alanine; Dithiothreitol; Endoplasmic Reticulum Stress; Gene Regulatory Networks; HeLa Cells; Humans; Isotope Labeling; Liquid-Liquid Extraction; Nonsense Mediated mRNA Decay; Proteomics; Tandem Mass Spectrometry; Unfolded Protein Response

2016
Quantifying Current Events Identifies a Novel Endurance Regulator.
    Trends in microbiology, 2016, Volume: 24, Issue:5

    Topics: Alanine; Amino Acids; Bacteria; Bacterial Proteins; Biofilms; Microbiological Techniques; Proteome; Pseudomonas aeruginosa

2016
Monitoring mRNA Translation in Neuronal Processes Using Fluorescent Non-Canonical Amino Acid Tagging.
    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 2016, Volume: 64, Issue:5

    Topics: Alanine; Alkynes; Amino Acids; Animals; Axons; Azides; Cerebral Cortex; Click Chemistry; Dendrites; Disks Large Homolog 4 Protein; Embryo, Mammalian; Fluorescent Dyes; Glycine; Intracellular Signaling Peptides and Proteins; Lab-On-A-Chip Devices; Membrane Proteins; Neurons; Primary Cell Culture; Protein Biosynthesis; Rats, Wistar; RNA, Messenger; Triazoles

2016
Quantitative chemical proteomics profiling of de novo protein synthesis during starvation-mediated autophagy.
    Autophagy, 2016, 10-02, Volume: 12, Issue:10

    Topics: Alanine; Autophagy; Gene Ontology; HeLa Cells; Humans; Protein Biosynthesis; Proteomics; Reproducibility of Results; Signal Transduction; Staining and Labeling; Transcription, Genetic

2016
Study of the degradation of a multidrug transporter using a non-radioactive pulse chase method.
    Analytical and bioanalytical chemistry, 2016, Volume: 408, Issue:27

    Topics: Alanine; Anti-Bacterial Agents; Bacterial Outer Membrane Proteins; Click Chemistry; Drug Resistance, Multiple, Bacterial; Escherichia coli; Escherichia coli Proteins; Gene Expression Regulation, Bacterial; Lipoproteins; Membrane Transport Proteins; Multidrug Resistance-Associated Proteins; Protein Binding; Protein Stability; Proteolysis; Staining and Labeling; Sulfur Radioisotopes

2016
Incorporation of non-canonical amino acids into the developing murine proteome.
    Scientific reports, 2016, 08-30, Volume: 6

    Topics: Alanine; Alkynes; Amino Acids; Animals; Embryonic Development; Female; Glycine; Methionine; Mice; Pregnancy; Protein Biosynthesis; Proteome; Proteomics

2016
Bioorthogonal Modification of the Major Sheath Protein of Bacteriophage M13: Extending the Versatility of Bionanomaterial Scaffolds.
    Bioconjugate chemistry, 2016, Oct-19, Volume: 27, Issue:10

    Topics: Alanine; Alkynes; Azides; Bacteriophage M13; Biotin; Cycloaddition Reaction; Fluorescent Dyes; Gold; Microscopy, Electron, Transmission; Nanoparticles; Rhodamines; Spectrometry, Mass, Electrospray Ionization; Viral Proteins

2016
Kinetic Analysis of Protein Stability Reveals Age-Dependent Degradation.
    Cell, 2016, Oct-20, Volume: 167, Issue:3

    Topics: Alanine; Aneuploidy; Cell Line; Click Chemistry; Gene Amplification; Humans; Kinetics; Markov Chains; Proteasome Endopeptidase Complex; Protein Biosynthesis; Protein Stability; Proteins; Proteolysis; Proteome; Ubiquitin

2016
Nitrilase-Activatable Noncanonical Amino Acid Precursors for Cell-Selective Metabolic Labeling of Proteomes.
    ACS chemical biology, 2016, 12-16, Volume: 11, Issue:12

    Topics: Alanine; Alkynes; Aminohydrolases; Animals; CHO Cells; Cricetulus; Glycine; HeLa Cells; Humans; Microscopy, Fluorescence; Proteome; Proteomics; Staining and Labeling

2016
Spatiotemporal fluorescence imaging of newly synthesized proteins in normal and cancerous cells with anticarcinogen modulation.
    Talanta, 2017, Jan-01, Volume: 162

    Topics: A549 Cells; Alanine; Amino Acids; Anticarcinogenic Agents; Cell Line; Cell Line, Tumor; Click Chemistry; Fluorescence; Humans; MCF-7 Cells; Microscopy, Confocal; Models, Chemical; Protein Biosynthesis; Proteins

2017
High fidelity visualization of cell-to-cell variation and temporal dynamics in nascent extracellular matrix formation.
    Scientific reports, 2016, 12-12, Volume: 6

    Topics: Alanine; Alkynes; Animals; Cattle; Cellular Microenvironment; Chondrocytes; Chondrogenesis; Extracellular Matrix; Extracellular Matrix Proteins; Fluorescent Antibody Technique, Direct; Fluorescent Dyes; Glycine; Mesenchymal Stem Cells; Microscopy, Confocal; Microscopy, Fluorescence; Quinolinium Compounds; Single-Cell Analysis

2016
Nonradioactive quantification of autophagic protein degradation with L-azidohomoalanine labeling.
    Nature protocols, 2017, Volume: 12, Issue:2

    Topics: Alanine; Alkynes; Animals; Autophagy; Azides; Cell Line; Click Chemistry; Fluorescent Dyes; Mice; Proteins; Proteolysis; Staining and Labeling

2017
Bioorthogonal Noncanonical Amino Acid Tagging (BONCAT) Enables Time-Resolved Analysis of Protein Synthesis in Native Plant Tissue.
    Plant physiology, 2017, Volume: 173, Issue:3

    Topics: Alanine; Arabidopsis; Arabidopsis Proteins; Azides; Chromatography, Liquid; Coloring Agents; Droughts; Hot Temperature; Immunoblotting; Light; Protein Biosynthesis; Reproducibility of Results; Seedlings; Sodium Chloride; Staining and Labeling; Stress, Physiological; Tandem Mass Spectrometry; Time Factors

2017
Proteomic Profiling of De Novo Protein Synthesis in Starvation-Induced Autophagy Using Bioorthogonal Noncanonical Amino Acid Tagging.
    Methods in enzymology, 2017, Volume: 588

    Topics: Alanine; Amino Acids; Animals; Autophagy; Cell Culture Techniques; Chromatography, Ion Exchange; Click Chemistry; HeLa Cells; Humans; Protein Biosynthesis; Proteins; Proteomics; Spectrometry, Mass, Electrospray Ionization

2017
A New Methodology for Incorporating Chiral Linkers into Stapled Peptides.
    Chembiochem : a European journal of chemical biology, 2017, 06-19, Volume: 18, Issue:12

    Topics: Alanine; Benzhydryl Compounds; Cell Line, Tumor; Cross-Linking Reagents; Humans; Peptide Library; Peptides; Protein Binding; Protein Stability; Protein Structure, Secondary; Proto-Oncogene Proteins c-mdm2; Solid-Phase Synthesis Techniques; Tumor Suppressor Protein p53

2017
HILAQ: A Novel Strategy for Newly Synthesized Protein Quantification.
    Journal of proteome research, 2017, 06-02, Volume: 16, Issue:6

    Topics: Alanine; Cell Death; HEK293 Cells; Humans; Isotope Labeling; Neurodegenerative Diseases; Oxidative Stress; Protein Biosynthesis; Proteins; Proteome

2017
Impact of Azidohomoalanine Incorporation on Protein Structure and Ligand Binding.
    Chembiochem : a European journal of chemical biology, 2017, 12-05, Volume: 18, Issue:23

    Topics: Alanine; Amino Acid Sequence; Circular Dichroism; Crystallography, X-Ray; Disks Large Homolog 4 Protein; Isotope Labeling; Ligands; Magnetic Resonance Spectroscopy; Mutagenesis; PDZ Domains; Peptides; Protein Binding; Protein Structure, Tertiary; Recombinant Proteins

2017
Integrating Two Efficient and Specific Bioorthogonal Ligation Reactions with Natural Metabolic Incorporation in One Cell for Virus Dual Labeling.
    Analytical chemistry, 2017, 11-07, Volume: 89, Issue:21

    Topics: Alanine; Animals; Capsid; Chlorocebus aethiops; Click Chemistry; DNA, Viral; Fluorescent Dyes; Nucleic Acid Conformation; Staining and Labeling; Vaccinia virus; Vero Cells

2017
Self-Directed in Cell Production of Methionine Analogue Azidohomoalanine by Synthetic Metabolism and Its Incorporation into Model Proteins.
    Methods in molecular biology (Clifton, N.J.), 2018, Volume: 1728

    Topics: Alanine; Amino Acids; Escherichia coli; Gene Expression; Metabolic Engineering; Protein Biosynthesis; Protein Engineering; Proteins; Recombinant Proteins

2018
Isolation of Primary Mouse Hepatocytes for Nascent Protein Synthesis Analysis by Non-radioactive L-azidohomoalanine Labeling Method.
    Journal of visualized experiments : JoVE, 2018, 10-23, Issue:140

    Topics: Alanine; Animals; Cells, Cultured; Hepatocytes; Mice; Proteins

2018
Targeted delivery and endosomal cellular uptake of DARPin-siRNA bioconjugates: Influence of linker stability on gene silencing.
    European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V, 2019, Volume: 141

    Topics: Alanine; Cell Line, Tumor; Click Chemistry; Endosomes; Epithelial Cell Adhesion Molecule; Gene Silencing; HeLa Cells; Humans; Maleimides; MCF-7 Cells; Muscle Proteins; Nuclear Proteins; RNA, Small Interfering; Sulfhydryl Compounds; Triazoles

2019
Quantitative analysis of global protein stability rates in tissues.
    Scientific reports, 2020, 09-29, Volume: 10, Issue:1

    Topics: Age Factors; Alanine; Animals; Brain; Click Chemistry; Liver; Male; Mass Spectrometry; Mice; Organ Specificity; Protein Stability; Proteolysis; Proteome; Proteostasis

2020
A terminal alkyne and disulfide functionalized agarose resin specifically enriches azidohomoalanine labeled nascent proteins.
    Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 2021, Feb-15, Volume: 1165

    Topics: Alanine; Alkynes; Chromatography, Liquid; Click Chemistry; Disulfides; HeLa Cells; Humans; Proteins; Proteome; Sepharose; Tandem Mass Spectrometry

2021
Site-selective dynamics of azidolysozyme.
    The Journal of chemical physics, 2021, Apr-28, Volume: 154, Issue:16

    Topics: Alanine; Azides; Hydrophobic and Hydrophilic Interactions; Molecular Dynamics Simulation; Muramidase; Thermodynamics

2021
In vivo homopropargylglycine incorporation enables sampling, isolation and characterization of nascent proteins from Arabidopsis thaliana.
    The Plant journal : for cell and molecular biology, 2021, Volume: 107, Issue:4

    Topics: Alanine; Alkynes; Arabidopsis; Arabidopsis Proteins; Gene Ontology; Glycine; Mass Spectrometry; Methionine; Nitrogen Isotopes; Plant Cells; Protein Processing, Post-Translational; Proteomics

2021
A flow-cytometry-based assessment of global protein synthesis in human senescent cells.
    STAR protocols, 2021, 09-17, Volume: 2, Issue:3

    Topics: Alanine; Animals; Cell Line; Cellular Senescence; Click Chemistry; Fibroblasts; Flow Cytometry; HEK293 Cells; Humans; Mice; Protein Biosynthesis; Proteins

2021
Proteome-wide mapping of short-lived proteins in human cells.
    Molecular cell, 2021, 11-18, Volume: 81, Issue:22

    Topics: Alanine; Cell Line; Cell Line, Tumor; Cycloheximide; Fucose; Geminin; HCT116 Cells; HEK293 Cells; Humans; Peptides; Principal Component Analysis; Protein Biosynthesis; Proteins; Proteome; Proteomics; Quality Control; RNA, Small Interfering; Telomere

2021
Modulation of cellular transcriptome and proteome composition by azidohomoalanine-implications on click chemistry-based secretome analysis.
    Journal of molecular medicine (Berlin, Germany), 2023, Volume: 101, Issue:7

    Topics: Alanine; Azides; Click Chemistry; Proteome; Secretome; Transcriptome

2023
Photo-Methionine, Azidohomoalanine and Homopropargylglycine Are Incorporated into Newly Synthesized Proteins at Different Rates and Differentially Affect the Growth and Protein Expression Levels of Auxotrophic and Prototrophic
    International journal of molecular sciences, 2023, Jul-22, Volume: 24, Issue:14

    Topics: Alanine; Amino Acids; Escherichia coli; Methionine; Proteins; Racemethionine

2023