alanine has been researched along with azidohomoalanine in 71 studies
Timeframe | Studies, this research(%) | All Research% |
---|---|---|
pre-1990 | 0 (0.00) | 18.7374 |
1990's | 0 (0.00) | 18.2507 |
2000's | 9 (12.68) | 29.6817 |
2010's | 54 (76.06) | 24.3611 |
2020's | 8 (11.27) | 2.80 |
Authors | Studies |
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Link, AJ; Tirrell, DA | 1 |
Back, JW; David, O; de Jong, L; de Koning, LJ; de Koster, CG; Kasper, PT; Kramer, G; Masson, G; van Maarseveen, JH | 1 |
Dieterich, DC; Graumann, J; Lee, JJ; Link, AJ; Schuman, EM; Tirrell, DA | 1 |
Link, AJ; Tirrell, DA; Vink, MK | 2 |
Grabstein, K; Johnson, RS; Tirrell, DA; Wang, A; Winblade Nairn, N | 1 |
Morimoto, J; Murakami, H; Sako, Y; Suga, H | 1 |
Back, J; de Jong, L; de Koning, LJ; de Koster, CG; Dekker, HL; Hellingwerf, KJ; Kramer, G; Nessen, MA; Sprenger, RR; van Maarseveen, JH | 1 |
Back, J; Baskin, JM; Bertozzi, CR; de Jong, L; de Koning, LJ; de Koster, CG; Hiemstra, H; Kramer, G; Nessen, MA; Smeenk, LE; van Maarseveen, JH | 1 |
Deal, RB; Henikoff, JG; Henikoff, S | 1 |
Dieterich, DC; Gouzer, G; Hodas, JJ; Ngo, JT; Schuman, EM; Shadrin, IY; Tirrell, DA; Triller, A | 1 |
Deal, RB; Henikoff, S | 1 |
Back, J; de Jong, L; de Koster, CG; de Mattos, MJ; Kramer, G; Nessen, MA; Roseboom, W; Speijer, D; Sprenger, RR | 1 |
Banerjee, PS; Carrico, I; Chung, J; Dyer, RB; Nagarajan, S; Raleigh, DP; Taskent-Sezgin, H | 1 |
Burnham, DR; Chiu, DT; Jin, Y; Schneider, T; Smith, PB; Wu, C | 1 |
Drewe, WC; Roth, S; Thomas, NR | 1 |
Garner, AL; Janda, KD; Kaufmann, GF; Kim, SK; Kravchenko, VV; Lowery, CA; Mayorov, AV; Park, J; Struss, AK; Yu, J; Zhu, J | 1 |
David, L; Simon, RP; Zhou, A | 1 |
Patel, KG; Swartz, JR | 1 |
de Jong, L; de Koster, CG; Kasper, PT; Kramer, G | 1 |
Banerjee, PS; Carrico, IS | 1 |
Plückthun, A; Simon, M; Zangemeister-Wittke, U | 1 |
Choi, KY; Ezzatti, P; Lippert, DN; Mookherjee, N | 1 |
Budisa, N; Lee, SG; Paik, HJ; Raghunathan, G; Sokalingam, S; Soundrarajan, N; Yoo, TH | 1 |
Bloem, R; Buchli, B; Hamm, P; Jelesarov, I; Koziol, K; Samatanga, B; Waldauer, SA; Walser, R | 1 |
Agris, PF; Fanning, KD; Francis, SP; Harris, KA; Katz, J; Lacy, M; Nicholas, BD; Pagana, J; Shin, JB | 1 |
Eichelbaum, K; Krijgsveld, J | 1 |
Bredenbeck, J; Müller-Werkmeister, HM | 1 |
Biava, H; Budisa, N; Contestabile, R; di Salvo, ML; Ma, Y | 1 |
Banister, S; Chew, YL; Götz, J; Kassiou, M; Lam, H; Liang, V; Nicholas, HR; Song, X; Ullrich, M; Zaw, T | 1 |
Bian, F; David, L; Frankel, M; Hall, CL; Li, Y; Simon, RP; Wainwright, J; Zhou, A | 1 |
Babin, BM; Hatzenpichler, R; Orphan, VJ; Scheller, S; Tavormina, PL; Tirrell, DA | 1 |
Lin, Q; Ng, S; Shen, HM; Wang, J; Zhang, J | 1 |
Banister, S; Berber, S; Chew, YL; Götz, J; Kassiou, M; Lam, H; Liang, V; Nicholas, HR; Song, X; Ullrich, M; Zaw, T | 1 |
Ballikaya, S; Gebert, J; Kopitz, J; Lee, J; Schnölzer, M; Warnken, U | 1 |
Bowling, H; Chao, MV; Hom, N; Kirshenbaum, K; Klann, E; Neubert, TA; Zhang, G | 1 |
Antileo, ER; Dieterich, DC; Landgraf, P; Schuman, EM | 1 |
Chang, YC; Chen, EC; Chung, HW; Fang, W; Hsu, WL; Lee, YT; Wu, CY; Wu, HI | 1 |
Hellberg, K; Liu, C; Ma, Y; Martínez-Bartolomé, S; McClatchy, DB; Shaw, RJ; Stein, BD; Vasquez, D; Yates, JR | 1 |
Budisa, N; Haag, R; Ma, Y; Thota, BN | 1 |
Dieterich, DC; Freitag, CE; Landgraf, P; Müller, A; Stellmacher, A | 1 |
Bhuvanagiri, M; Hauer, C; Hentze, MW; Krijgsveld, J; Kulozik, AE; Leicht, S; Neu-Yilik, G; Sieber, J | 1 |
Brynildsen, MP; Henry, TC | 1 |
Aschrafi, A; Gioio, A; Kaplan, BB; Kos, A; Martens, GJ; Wanke, KA | 1 |
Bao, F; Koh, PL; Lee, YM; Lin, Q; Ng, S; Shen, HM; Wang, J; Zhang, J | 1 |
Chai, Q; Dutch, RE; Wang, Z; Webb, SR; Wei, Y | 1 |
Calve, S; Kinzer-Ursem, TL; Ocken, AR; Witten, AJ | 1 |
Daub, E; Honek, JF; Urquhart, T | 1 |
Chen, W; Donnelly, N; Hou, J; Marsh, JA; McShane, E; Selbach, M; Sin, C; Storchova, Z; Valleriani, A; Wang, X; Wells, JN; Zauber, H | 1 |
Chen, X; Li, Z; Liu, W; Qin, K; Sun, Y; Zhou, W; Zhu, Y | 1 |
Sheng, L; Wang, J; Zhang, S; Zhang, X; Zhao, H | 1 |
Mauck, RL; McLeod, CM | 1 |
Hua, ZC; Lee, YM; Lin, Q; Ng, S; Shen, HM; Shi, Y; Wang, J; Zhang, J | 1 |
Bailey-Serres, J; Glenn, WS; Hess, S; Ho, SH; Moradian, A; Stone, SE; Sweredoski, MJ; Tirrell, DA | 1 |
Lee, YM; Lim, TK; Lin, Q; Shen, HM; Wang, J; Zhang, J | 1 |
Itzhaki, LS; Ley, SV; Serrano, JC; Sipthorp, J; Xu, W | 1 |
Barkallah, S; Ma, Y; McClatchy, DB; Wood, WW; Yates, JR | 1 |
Bredenbeck, J; Eberl, KB; Kudlinzki, D; Lehner, F; Müller-Werkmeister, HM; Richter, C; Schwalbe, H; Silvers, R | 1 |
Huang, LL; Liu, K; Xie, HY; Xu, J; Zhang, Q; Zhao, D; Zhu, H | 1 |
Budisa, N; Di Salvo, ML; Ma, Y | 1 |
Ma, Y; Yates, JR | 1 |
Bernhard, E; Bethi, M; Borra, V; Murakami, K; Nakamura, T; Salem, ESB; Takahashi, T | 1 |
Brandl, F; Jones, AT; Lorenzer, C; Merten, H; Plückthun, A; Sayers, EJ; Streußnig, S; Tot, E; Watson, P; Winkler, AM; Winkler, J; Zangemeister-Wittke, U | 1 |
Gao, Y; Lavallée-Adam, M; Martínez-Bartolomé, S; McClatchy, DB; Yates, JR | 1 |
Ghazanfar, S; Han, X; Hou, Z; Liu, H; Wang, Z; Yang, J | 1 |
Meuwly, M; Salehi, SM | 1 |
Duncan, O; Fenske, R; Millar, AH; Tivendale, ND | 1 |
Jeon, T; Kang, C; Kim, J; Kim, MS; Lee, Y; Roh, K | 1 |
Cai, Z; Gygi, SP; Harry, BL; Huttlin, EL; Li, J; Mitchell, DC; Paulo, JA; Shen, N; Vaites, LP | 1 |
Arnold-Schild, D; Bopp, T; Chen, Y; Distler, U; Kirschner, F; Klein, M; Kücük, C; Leps, C; Ludt, A; Marini, F; Michna, T; Rajalingam, K; Riegel, K; Schild, H; Sielaff, M; Stein, L; Tenzer, S; Łącki, MK | 1 |
Jecmen, T; Sulc, M; Tuzhilkin, R | 1 |
1 review(s) available for alanine and azidohomoalanine
Article | Year |
---|---|
Proteomics and pulse azidohomoalanine labeling of newly synthesized proteins: what are the potential applications?
Topics: Alanine; Animals; Humans; Immunity; Isotope Labeling; Microbiota; Protein Biosynthesis; Proteomics; Proteostasis | 2018 |
1 trial(s) available for alanine and azidohomoalanine
Article | Year |
---|---|
Nascent proteomes in peripheral blood mononuclear cells as a novel source for biomarker discovery in human stroke.
Topics: Alanine; Biomarkers; Cells, Cultured; Cluster Analysis; Female; Humans; Leukocytes, Mononuclear; Male; Mass Spectrometry; Pilot Projects; Proteome; Proteomics; Staining and Labeling; Stroke | 2014 |
69 other study(ies) available for alanine and azidohomoalanine
Article | Year |
---|---|
Cell surface labeling of Escherichia coli via copper(I)-catalyzed [3+2] cycloaddition.
Topics: Alanine; Catalysis; Copper; Escherichia coli; Gene Expression; Membrane Proteins; Models, Chemical; Molecular Structure; Mutagenesis, Site-Directed; Mutation; Porins; Staining and Labeling | 2003 |
Mild and chemoselective peptide-bond cleavage of peptides and proteins at azido homoalanine.
Topics: Alanine; Amino Acid Sequence; Azides; Molecular Sequence Data; Molecular Structure; Peptides; Phosphines; Proteins | 2005 |
Labeling, detection and identification of newly synthesized proteomes with bioorthogonal non-canonical amino-acid tagging.
Topics: Affinity Labels; Alanine; Amino Acids; Animals; Azides; Gene Expression Profiling; Mice; Proteins; Proteomics | 2007 |
Preparation of the functionalizable methionine surrogate azidohomoalanine via copper-catalyzed diazo transfer.
Topics: Alanine; Biochemistry; Butyrates; Catalysis; Chromatography, Ion Exchange; Copper; Crystallization; Furans; Methionine; Sulfonamides | 2007 |
Synthesis of the functionalizable methionine surrogate azidohomoalanine using Boc-homoserine as precursor.
Topics: Alanine; Biochemistry; Chromatography, Ion Exchange; Crystallization; Formic Acid Esters; Homoserine | 2007 |
Processing of N-terminal unnatural amino acids in recombinant human interferon-beta in Escherichia coli.
Topics: Alanine; Amino Acid Sequence; Amino Acid Substitution; Amino Acids; Base Sequence; Binding Sites; Electrophoresis, Polyacrylamide Gel; Escherichia coli; Glutamic Acid; Glycine; Histidine; Humans; Interferon Type I; Molecular Sequence Data; Protein Engineering; Recombinant Proteins; Serine; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Substrate Specificity | 2008 |
Ribosomal synthesis of bicyclic peptides via two orthogonal inter-side-chain reactions.
Topics: Alanine; Alkynes; Amino Acid Sequence; Amino Acids; Aminobutyrates; Codon; Cysteine; Glycine; Peptides, Cyclic; Ribosomes; RNA, Messenger; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Templates, Genetic | 2008 |
Identification and quantitation of newly synthesized proteins in Escherichia coli by enrichment of azidohomoalanine-labeled peptides with diagonal chromatography.
Topics: Alanine; Chromatography; Chromatography, Liquid; Escherichia coli; Escherichia coli Proteins; Methionine; Molecular Structure; Peptides; Tandem Mass Spectrometry | 2009 |
Selective enrichment of azide-containing peptides from complex mixtures.
Topics: Alanine; Amino Acid Sequence; Azides; Cations; Chromatography, Ion Exchange; Cross-Linking Reagents; Cytochromes c; Glutarates; Kinetics; Mass Spectrometry; Methionine; Molecular Sequence Data; Peptides; Proteome; Proteomics | 2009 |
Genome-wide kinetics of nucleosome turnover determined by metabolic labeling of histones.
Topics: Alanine; Animals; Cell Line; Drosophila melanogaster; Drosophila Proteins; Genome, Insect; Histones; Kinetics; Methionine; Molecular Probe Techniques; Nucleosomes; Oligonucleotide Array Sequence Analysis | 2010 |
In situ visualization and dynamics of newly synthesized proteins in rat hippocampal neurons.
Topics: Alanine; Alkynes; Amino Acids; Animals; Cells, Cultured; Fluorescent Dyes; Glycine; Hippocampus; Immunohistochemistry; Microdialysis; Nerve Tissue Proteins; Neurons; Protein Biosynthesis; Proteomics; Quantum Dots; Rats; Rats, Sprague-Dawley; Staining and Labeling | 2010 |
Catching a glimpse of nucleosome dynamics.
Topics: Alanine; Animals; DNA; Drosophila; Histones; Nucleosomes; Polycomb-Group Proteins; Repressor Proteins | 2010 |
Proteome-wide alterations in Escherichia coli translation rates upon anaerobiosis.
Topics: Alanine; Anaerobiosis; Escherichia coli; Escherichia coli Proteins; Gene Expression Regulation, Bacterial; Protein Biosynthesis; Proteome | 2010 |
Azidohomoalanine: a conformationally sensitive IR probe of protein folding, protein structure, and electrostatics.
Topics: Alanine; Models, Molecular; Protein Conformation; Protein Folding; Protein Structure, Tertiary; Proteins; Ribosomal Proteins; Spectrophotometry, Infrared; Static Electricity | 2010 |
Ultrabright and bioorthogonal labeling of cellular targets using semiconducting polymer dots and click chemistry.
Topics: 3T3 Cells; Alanine; Animals; Click Chemistry; Fluorenes; Fluorescent Dyes; Humans; Mice; Molecular Structure; Particle Size; Polymers; Proteins; Semiconductors; Staining and Labeling; Surface Properties; Tumor Cells, Cultured | 2010 |
A concise and scalable route to L-azidohomoalanine.
Topics: Alanine; Aspartic Acid; Molecular Structure; Proteins | 2010 |
Synthesis of 'clickable' acylhomoserine lactone quorum sensing probes: unanticipated effects on mammalian cell activation.
Topics: Alanine; Animals; Cells; Click Chemistry; Homoserine; Humans; Lactones; Molecular Structure; Pseudomonas; Quorum Sensing | 2011 |
Nascent proteomes of ischemic-injured and ischemic-tolerant neuronal cells.
Topics: Alanine; Animals; Brain; Brain Ischemia; Cells, Cultured; Click Chemistry; Down-Regulation; Gene Expression Profiling; Mice; Neurons; Proteome; Proteomics; Up-Regulation | 2011 |
Surface functionalization of virus-like particles by direct conjugation using azide-alkyne click chemistry.
Topics: Alanine; Alkynes; Animals; Antigens; Cell Line; Cell-Free System; Click Chemistry; CpG Islands; DNA; Glycine; Granulocyte-Macrophage Colony-Stimulating Factor; Immunoglobulin Idiotypes; Lymphoma, B-Cell; Mice; Polyethylene Glycols; Substrate Specificity; Surface Properties; Vaccines, Virus-Like Particle | 2011 |
Quantitation of newly synthesized proteins by pulse labeling with azidohomoalanine.
Topics: Alanine; Chromatography; Chromatography, Liquid; Escherichia coli; Humans; Molecular Structure; Protein Biosynthesis; Proteins; Proteomics; Software; Staining and Labeling; Tandem Mass Spectrometry | 2011 |
Chemoselective modification of viral proteins bearing metabolically introduced "clickable" amino acids and sugars.
Topics: Adenoviridae; Alanine; Alkynes; Amino Acids; Animals; Azides; Carbohydrate Metabolism; Carbohydrates; Catalysis; Click Chemistry; Copper; Electrophoresis, Polyacrylamide Gel; Rhodamines; Staining and Labeling; Substrate Specificity; Viral Proteins | 2011 |
Facile double-functionalization of designed ankyrin repeat proteins using click and thiol chemistries.
Topics: Alanine; Ankyrin Repeat; Cell Adhesion Molecules; Click Chemistry; Epithelial Cells; Fluorescent Dyes; Humans; Hydrolysis; Kinetics; Models, Molecular; Molecular Structure; Polyethylene Glycols; Proteins; Sulfhydryl Compounds; Tumor Cells, Cultured | 2012 |
Defining TNF-α and IL-1β induced nascent proteins: combining bio-orthogonal non-canonical amino acid tagging and proteomics.
Topics: Alanine; Cell Line; Humans; Interleukin-1beta; Mass Spectrometry; Membrane Transport Proteins; Monocytes; Proteome; Proteomics; Tumor Necrosis Factor-alpha | 2012 |
Conjugation of proteins by installing BIO-orthogonally reactive groups at their N-termini.
Topics: Alanine; Alkynes; Click Chemistry; Glycine; Green Fluorescent Proteins; Methionine; Proteins | 2012 |
Ligand binding studied by 2D IR spectroscopy using the azidohomoalanine label.
Topics: Alanine; Ligands; Models, Molecular; Spectrophotometry, Infrared; Staining and Labeling | 2012 |
A novel role of cytosolic protein synthesis inhibition in aminoglycoside ototoxicity.
Topics: Alanine; Alkynes; Aminoglycosides; Animals; Anti-Bacterial Agents; Apoptosis; Blotting, Western; Cell Count; Chick Embryo; Cytosol; Ear Diseases; Enzyme Activation; Evoked Potentials, Auditory, Brain Stem; Glycine; Hair Cells, Auditory; Immunohistochemistry; JNK Mitogen-Activated Protein Kinases; Mice; Mice, Inbred CBA; Niacinamide; Organ Culture Techniques; Phenylurea Compounds; Protein Kinase Inhibitors; Protein Synthesis Inhibitors; RNA, Ribosomal; Sorafenib | 2013 |
Rapid temporal dynamics of transcription, protein synthesis, and secretion during macrophage activation.
Topics: Alanine; Animals; Cell Line; Cell Membrane; Databases, Protein; Gene Expression Regulation; Isotope Labeling; Lipopolysaccharides; Macrophage Activation; Macrophages; Mice; Protein Biosynthesis; Proteolysis; Proteome; Proteomics; RNA, Messenger; Signal Transduction; Time Factors; Transcription Factors; Transcription, Genetic; Transcriptome | 2014 |
A donor-acceptor pair for the real time study of vibrational energy transfer in proteins.
Topics: Absorption; Alanine; Azulenes; Energy Transfer; Oligopeptides; Proteins; Vibration | 2014 |
Coupling bioorthogonal chemistries with artificial metabolism: intracellular biosynthesis of azidohomoalanine and its incorporation into recombinant proteins.
Topics: Alanine; Bacterial Proteins; Click Chemistry; Escherichia coli; Fermentation; Genetic Engineering; Metabolic Networks and Pathways; Protein Processing, Post-Translational; Recombinant Proteins; Spectrometry, Mass, Electrospray Ionization | 2014 |
Altered proteostasis in aging and heat shock response in C. elegans revealed by analysis of the global and de novo synthesized proteome.
Topics: Aging; Alanine; Animals; Blotting, Western; Caenorhabditis elegans; Caenorhabditis elegans Proteins; Click Chemistry; Forecasting; Gene Expression Regulation; Genes, Helminth; Heat-Shock Response; Hot Temperature; Mass Spectrometry; Microscopy, Fluorescence; Protein Folding; Proteome; Proteostasis Deficiencies | 2014 |
In situ visualization of newly synthesized proteins in environmental microbes using amino acid tagging and click chemistry.
Topics: Alanine; Alkynes; Archaea; Archaeal Proteins; Azides; Bacteria; Bacterial Proteins; Biofilms; Click Chemistry; Fresh Water; Geologic Sediments; Humans; In Situ Hybridization, Fluorescence; Methionine; Microscopy, Fluorescence; Mouth; Staining and Labeling | 2014 |
Development of a novel method for quantification of autophagic protein degradation by AHA labeling.
Topics: Alanine; Animals; Autophagy; Biological Assay; Cell Survival; Cells, Cultured; Flow Cytometry; Fluorescent Dyes; Hep G2 Cells; Humans; Mice; Proteolysis; Staining and Labeling | 2014 |
Bio-orthogonal labeling as a tool to visualize and identify newly synthesized proteins in Caenorhabditis elegans.
Topics: Alanine; Amino Acids; Animals; Blotting, Western; Caenorhabditis elegans Proteins; Chemistry Techniques, Analytical; Click Chemistry; Fluorescent Antibody Technique; Mass Spectrometry; Staining and Labeling | 2014 |
De Novo proteome analysis of genetically modified tumor cells by a metabolic labeling/azide-alkyne cycloaddition approach.
Topics: Activin Receptors, Type II; Alanine; Alkynes; Apoptosis; Apoptosis Regulatory Proteins; Azides; Biotin; Click Chemistry; Cycloaddition Reaction; Doxycycline; Gene Expression Profiling; Gene Expression Regulation, Neoplastic; Genetic Complementation Test; HCT116 Cells; Humans; Molecular Sequence Annotation; Proteome; Recombinases; Signal Transduction; Staining and Labeling; Streptavidin | 2014 |
In-depth quantitative proteomic analysis of de novo protein synthesis induced by brain-derived neurotrophic factor.
Topics: Alanine; Brain-Derived Neurotrophic Factor; Chromatography, Liquid; HEK293 Cells; Humans; Mass Spectrometry; Protein Biosynthesis; Proteome; Proteomics; Reproducibility of Results | 2014 |
BONCAT: metabolic labeling, click chemistry, and affinity purification of newly synthesized proteomes.
Topics: Alanine; Chromatography, Affinity; Click Chemistry; Cycloaddition Reaction; HEK293 Cells; Humans; Proteome; Staining and Labeling | 2015 |
Glutamate Stimulates Local Protein Synthesis in the Axons of Rat Cortical Neurons by Activating α-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid (AMPA) Receptors and Metabotropic Glutamate Receptors.
Topics: Alanine; alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid; Animals; Axons; Brain-Derived Neurotrophic Factor; Calcium Channels; Calcium Signaling; Embryo, Mammalian; Gene Expression Regulation, Developmental; Glutamic Acid; Hippocampus; Kinetics; Primary Cell Culture; Protein Biosynthesis; Rats; Rats, Sprague-Dawley; Receptors, AMPA; Receptors, Metabotropic Glutamate; Synapses; TOR Serine-Threonine Kinases; TRPV Cation Channels | 2015 |
Pulsed Azidohomoalanine Labeling in Mammals (PALM) Detects Changes in Liver-Specific LKB1 Knockout Mice.
Topics: Alanine; Alkynes; AMP-Activated Protein Kinases; Animals; Azides; Biotin; Click Chemistry; Food, Formulated; Gene Expression; Integrases; Liver; Male; Methionine; Mice; Mice, Inbred C57BL; Mice, Knockout; Molecular Sequence Annotation; Protein Serine-Threonine Kinases; Proteome; Proteomics | 2015 |
Dendronylation: Residue-specific chemoselective attachment of oligoglycerol dendrimers on proteins with noncanonical amino acids.
Topics: Alanine; Amino Acid Substitution; Azides; Bacillus; Bacterial Proteins; Cell Line; Click Chemistry; Dendrimers; Escherichia coli; Glycerol; Methionine; Recombinant Proteins | 2015 |
Monitoring Astrocytic Proteome Dynamics by Cell Type-Specific Protein Labeling.
Topics: Alanine; Animals; Astrocytes; Azides; Brain-Derived Neurotrophic Factor; Coculture Techniques; Green Fluorescent Proteins; HEK293 Cells; Humans; Methionine-tRNA Ligase; Neuroglia; Norleucine; Protein Biosynthesis; Proteins; Proteome; Proteomics; Rats, Wistar | 2015 |
Proteomic Analysis Reveals Branch-specific Regulation of the Unfolded Protein Response by Nonsense-mediated mRNA Decay.
Topics: Alanine; Dithiothreitol; Endoplasmic Reticulum Stress; Gene Regulatory Networks; HeLa Cells; Humans; Isotope Labeling; Liquid-Liquid Extraction; Nonsense Mediated mRNA Decay; Proteomics; Tandem Mass Spectrometry; Unfolded Protein Response | 2016 |
Quantifying Current Events Identifies a Novel Endurance Regulator.
Topics: Alanine; Amino Acids; Bacteria; Bacterial Proteins; Biofilms; Microbiological Techniques; Proteome; Pseudomonas aeruginosa | 2016 |
Monitoring mRNA Translation in Neuronal Processes Using Fluorescent Non-Canonical Amino Acid Tagging.
Topics: Alanine; Alkynes; Amino Acids; Animals; Axons; Azides; Cerebral Cortex; Click Chemistry; Dendrites; Disks Large Homolog 4 Protein; Embryo, Mammalian; Fluorescent Dyes; Glycine; Intracellular Signaling Peptides and Proteins; Lab-On-A-Chip Devices; Membrane Proteins; Neurons; Primary Cell Culture; Protein Biosynthesis; Rats, Wistar; RNA, Messenger; Triazoles | 2016 |
Quantitative chemical proteomics profiling of de novo protein synthesis during starvation-mediated autophagy.
Topics: Alanine; Autophagy; Gene Ontology; HeLa Cells; Humans; Protein Biosynthesis; Proteomics; Reproducibility of Results; Signal Transduction; Staining and Labeling; Transcription, Genetic | 2016 |
Study of the degradation of a multidrug transporter using a non-radioactive pulse chase method.
Topics: Alanine; Anti-Bacterial Agents; Bacterial Outer Membrane Proteins; Click Chemistry; Drug Resistance, Multiple, Bacterial; Escherichia coli; Escherichia coli Proteins; Gene Expression Regulation, Bacterial; Lipoproteins; Membrane Transport Proteins; Multidrug Resistance-Associated Proteins; Protein Binding; Protein Stability; Proteolysis; Staining and Labeling; Sulfur Radioisotopes | 2016 |
Incorporation of non-canonical amino acids into the developing murine proteome.
Topics: Alanine; Alkynes; Amino Acids; Animals; Embryonic Development; Female; Glycine; Methionine; Mice; Pregnancy; Protein Biosynthesis; Proteome; Proteomics | 2016 |
Bioorthogonal Modification of the Major Sheath Protein of Bacteriophage M13: Extending the Versatility of Bionanomaterial Scaffolds.
Topics: Alanine; Alkynes; Azides; Bacteriophage M13; Biotin; Cycloaddition Reaction; Fluorescent Dyes; Gold; Microscopy, Electron, Transmission; Nanoparticles; Rhodamines; Spectrometry, Mass, Electrospray Ionization; Viral Proteins | 2016 |
Kinetic Analysis of Protein Stability Reveals Age-Dependent Degradation.
Topics: Alanine; Aneuploidy; Cell Line; Click Chemistry; Gene Amplification; Humans; Kinetics; Markov Chains; Proteasome Endopeptidase Complex; Protein Biosynthesis; Protein Stability; Proteins; Proteolysis; Proteome; Ubiquitin | 2016 |
Nitrilase-Activatable Noncanonical Amino Acid Precursors for Cell-Selective Metabolic Labeling of Proteomes.
Topics: Alanine; Alkynes; Aminohydrolases; Animals; CHO Cells; Cricetulus; Glycine; HeLa Cells; Humans; Microscopy, Fluorescence; Proteome; Proteomics; Staining and Labeling | 2016 |
Spatiotemporal fluorescence imaging of newly synthesized proteins in normal and cancerous cells with anticarcinogen modulation.
Topics: A549 Cells; Alanine; Amino Acids; Anticarcinogenic Agents; Cell Line; Cell Line, Tumor; Click Chemistry; Fluorescence; Humans; MCF-7 Cells; Microscopy, Confocal; Models, Chemical; Protein Biosynthesis; Proteins | 2017 |
High fidelity visualization of cell-to-cell variation and temporal dynamics in nascent extracellular matrix formation.
Topics: Alanine; Alkynes; Animals; Cattle; Cellular Microenvironment; Chondrocytes; Chondrogenesis; Extracellular Matrix; Extracellular Matrix Proteins; Fluorescent Antibody Technique, Direct; Fluorescent Dyes; Glycine; Mesenchymal Stem Cells; Microscopy, Confocal; Microscopy, Fluorescence; Quinolinium Compounds; Single-Cell Analysis | 2016 |
Nonradioactive quantification of autophagic protein degradation with L-azidohomoalanine labeling.
Topics: Alanine; Alkynes; Animals; Autophagy; Azides; Cell Line; Click Chemistry; Fluorescent Dyes; Mice; Proteins; Proteolysis; Staining and Labeling | 2017 |
Bioorthogonal Noncanonical Amino Acid Tagging (BONCAT) Enables Time-Resolved Analysis of Protein Synthesis in Native Plant Tissue.
Topics: Alanine; Arabidopsis; Arabidopsis Proteins; Azides; Chromatography, Liquid; Coloring Agents; Droughts; Hot Temperature; Immunoblotting; Light; Protein Biosynthesis; Reproducibility of Results; Seedlings; Sodium Chloride; Staining and Labeling; Stress, Physiological; Tandem Mass Spectrometry; Time Factors | 2017 |
Proteomic Profiling of De Novo Protein Synthesis in Starvation-Induced Autophagy Using Bioorthogonal Noncanonical Amino Acid Tagging.
Topics: Alanine; Amino Acids; Animals; Autophagy; Cell Culture Techniques; Chromatography, Ion Exchange; Click Chemistry; HeLa Cells; Humans; Protein Biosynthesis; Proteins; Proteomics; Spectrometry, Mass, Electrospray Ionization | 2017 |
A New Methodology for Incorporating Chiral Linkers into Stapled Peptides.
Topics: Alanine; Benzhydryl Compounds; Cell Line, Tumor; Cross-Linking Reagents; Humans; Peptide Library; Peptides; Protein Binding; Protein Stability; Protein Structure, Secondary; Proto-Oncogene Proteins c-mdm2; Solid-Phase Synthesis Techniques; Tumor Suppressor Protein p53 | 2017 |
HILAQ: A Novel Strategy for Newly Synthesized Protein Quantification.
Topics: Alanine; Cell Death; HEK293 Cells; Humans; Isotope Labeling; Neurodegenerative Diseases; Oxidative Stress; Protein Biosynthesis; Proteins; Proteome | 2017 |
Impact of Azidohomoalanine Incorporation on Protein Structure and Ligand Binding.
Topics: Alanine; Amino Acid Sequence; Circular Dichroism; Crystallography, X-Ray; Disks Large Homolog 4 Protein; Isotope Labeling; Ligands; Magnetic Resonance Spectroscopy; Mutagenesis; PDZ Domains; Peptides; Protein Binding; Protein Structure, Tertiary; Recombinant Proteins | 2017 |
Integrating Two Efficient and Specific Bioorthogonal Ligation Reactions with Natural Metabolic Incorporation in One Cell for Virus Dual Labeling.
Topics: Alanine; Animals; Capsid; Chlorocebus aethiops; Click Chemistry; DNA, Viral; Fluorescent Dyes; Nucleic Acid Conformation; Staining and Labeling; Vaccinia virus; Vero Cells | 2017 |
Self-Directed in Cell Production of Methionine Analogue Azidohomoalanine by Synthetic Metabolism and Its Incorporation into Model Proteins.
Topics: Alanine; Amino Acids; Escherichia coli; Gene Expression; Metabolic Engineering; Protein Biosynthesis; Protein Engineering; Proteins; Recombinant Proteins | 2018 |
Isolation of Primary Mouse Hepatocytes for Nascent Protein Synthesis Analysis by Non-radioactive L-azidohomoalanine Labeling Method.
Topics: Alanine; Animals; Cells, Cultured; Hepatocytes; Mice; Proteins | 2018 |
Targeted delivery and endosomal cellular uptake of DARPin-siRNA bioconjugates: Influence of linker stability on gene silencing.
Topics: Alanine; Cell Line, Tumor; Click Chemistry; Endosomes; Epithelial Cell Adhesion Molecule; Gene Silencing; HeLa Cells; Humans; Maleimides; MCF-7 Cells; Muscle Proteins; Nuclear Proteins; RNA, Small Interfering; Sulfhydryl Compounds; Triazoles | 2019 |
Quantitative analysis of global protein stability rates in tissues.
Topics: Age Factors; Alanine; Animals; Brain; Click Chemistry; Liver; Male; Mass Spectrometry; Mice; Organ Specificity; Protein Stability; Proteolysis; Proteome; Proteostasis | 2020 |
A terminal alkyne and disulfide functionalized agarose resin specifically enriches azidohomoalanine labeled nascent proteins.
Topics: Alanine; Alkynes; Chromatography, Liquid; Click Chemistry; Disulfides; HeLa Cells; Humans; Proteins; Proteome; Sepharose; Tandem Mass Spectrometry | 2021 |
Site-selective dynamics of azidolysozyme.
Topics: Alanine; Azides; Hydrophobic and Hydrophilic Interactions; Molecular Dynamics Simulation; Muramidase; Thermodynamics | 2021 |
In vivo homopropargylglycine incorporation enables sampling, isolation and characterization of nascent proteins from Arabidopsis thaliana.
Topics: Alanine; Alkynes; Arabidopsis; Arabidopsis Proteins; Gene Ontology; Glycine; Mass Spectrometry; Methionine; Nitrogen Isotopes; Plant Cells; Protein Processing, Post-Translational; Proteomics | 2021 |
A flow-cytometry-based assessment of global protein synthesis in human senescent cells.
Topics: Alanine; Animals; Cell Line; Cellular Senescence; Click Chemistry; Fibroblasts; Flow Cytometry; HEK293 Cells; Humans; Mice; Protein Biosynthesis; Proteins | 2021 |
Proteome-wide mapping of short-lived proteins in human cells.
Topics: Alanine; Cell Line; Cell Line, Tumor; Cycloheximide; Fucose; Geminin; HCT116 Cells; HEK293 Cells; Humans; Peptides; Principal Component Analysis; Protein Biosynthesis; Proteins; Proteome; Proteomics; Quality Control; RNA, Small Interfering; Telomere | 2021 |
Modulation of cellular transcriptome and proteome composition by azidohomoalanine-implications on click chemistry-based secretome analysis.
Topics: Alanine; Azides; Click Chemistry; Proteome; Secretome; Transcriptome | 2023 |
Photo-Methionine, Azidohomoalanine and Homopropargylglycine Are Incorporated into Newly Synthesized Proteins at Different Rates and Differentially Affect the Growth and Protein Expression Levels of Auxotrophic and Prototrophic
Topics: Alanine; Amino Acids; Escherichia coli; Methionine; Proteins; Racemethionine | 2023 |