agar and sodium-metabisulfite

agar has been researched along with sodium-metabisulfite* in 2 studies

Other Studies

2 other study(ies) available for agar and sodium-metabisulfite

Article	Year
Biphasic culture system for rapid Campylobacter cultivation. Applied and environmental microbiology, 1983, Volume: 45, Issue:1 We developed a biphasic culture system consisting of 4 ml of brucella agar (BA) and 6 ml of brucella broth (BB) in 25-cm2 tissue culture flasks, which were incubated in air (BB/BAa) or in a gas mixture of 5% O2, 10% CO2, and 85% N2 (BB/BAg). These media were also used with a supplement consisting of ferrous sulfate, sodium metabisulfite, and sodium pyruvate and incubated as above (FB/FAa and FB/FAg, respectively). Highly satisfactory growth of Campylobacter jejuni 301 was obtained with all medium-gas phase combinations provided that the number of viable cells in the inoculum was large (greater than or equal to 10(6)/ml). The use of FB/FAa permitted the inoculum to be reduced to 100 cells per ml. With an adjusted gas phase (BB/BAg and FB/FAg), near-optimal growth was obtained from an inoculum of 1 to 10 cells per ml. Under most of these conditions the generation time was approximately 90 min. During the logarithmic growth phase, the cells retained their typical spiral morphology and high motility. These media also proved to be highly satisfactory for the cultivation of fresh isolates as well as other stock strains of Campylobacter. When the broth phase of the cultures, after addition of 15% glycerol, was quickly frozen and maintained at -70 degrees C, all strains thus far examined were readily recoverable and satisfactorily cultivated without additional passage. Topics: Agar; Campylobacter; Campylobacter fetus; Carbon Dioxide; Culture Media; Ferrous Compounds; Hydrogen-Ion Concentration; Movement; Nitrogen; Oxygen; Pyruvates; Pyruvic Acid; Sulfites	1983
[Determination of the formation of hydrogen sulfide in a dry differentiating medium of the Kligler agar type]. Zhurnal mikrobiologii, epidemiologii i immunobiologii, 1983, Issue:4 The indicator system for hydrogen sulfide detection in media analogous to Kligler's agar, intended for the primary identification of enterobacteria, has been studied. The indicator system under study, in contrast to the known formulas, contains sodium metabisulfite for improving the capacity for detecting hydrogen sulfide production in bacteria which produce it in small amounts from inorganic sulfur compounds. Topics: Agar; Culture Media; Enterobacteriaceae; Hydrogen Sulfide; Sulfites	1983

Article

Year

Biphasic culture system for rapid Campylobacter cultivation.

Applied and environmental microbiology, 1983, Volume: 45, Issue:1

We developed a biphasic culture system consisting of 4 ml of brucella agar (BA) and 6 ml of brucella broth (BB) in 25-cm2 tissue culture flasks, which were incubated in air (BB/BAa) or in a gas mixture of 5% O2, 10% CO2, and 85% N2 (BB/BAg). These media were also used with a supplement consisting of ferrous sulfate, sodium metabisulfite, and sodium pyruvate and incubated as above (FB/FAa and FB/FAg, respectively). Highly satisfactory growth of Campylobacter jejuni 301 was obtained with all medium-gas phase combinations provided that the number of viable cells in the inoculum was large (greater than or equal to 10(6)/ml). The use of FB/FAa permitted the inoculum to be reduced to 100 cells per ml. With an adjusted gas phase (BB/BAg and FB/FAg), near-optimal growth was obtained from an inoculum of 1 to 10 cells per ml. Under most of these conditions the generation time was approximately 90 min. During the logarithmic growth phase, the cells retained their typical spiral morphology and high motility. These media also proved to be highly satisfactory for the cultivation of fresh isolates as well as other stock strains of Campylobacter. When the broth phase of the cultures, after addition of 15% glycerol, was quickly frozen and maintained at -70 degrees C, all strains thus far examined were readily recoverable and satisfactorily cultivated without additional passage.

Topics: Agar; Campylobacter; Campylobacter fetus; Carbon Dioxide; Culture Media; Ferrous Compounds; Hydrogen-Ion Concentration; Movement; Nitrogen; Oxygen; Pyruvates; Pyruvic Acid; Sulfites

1983

[Determination of the formation of hydrogen sulfide in a dry differentiating medium of the Kligler agar type].

Zhurnal mikrobiologii, epidemiologii i immunobiologii, 1983, Issue:4

The indicator system for hydrogen sulfide detection in media analogous to Kligler's agar, intended for the primary identification of enterobacteria, has been studied. The indicator system under study, in contrast to the known formulas, contains sodium metabisulfite for improving the capacity for detecting hydrogen sulfide production in bacteria which produce it in small amounts from inorganic sulfur compounds.

Topics: Agar; Culture Media; Enterobacteriaceae; Hydrogen Sulfide; Sulfites

1983