agar and lactobionic-acid

agar has been researched along with lactobionic-acid* in 2 studies

Other Studies

2 other study(ies) available for agar and lactobionic-acid

ArticleYear
Efficient isolation of new lactobionic acid-producing microorganisms from environmental samples by colloidal calcium carbonate agar plate-based screening.
    Bioprocess and biosystems engineering, 2022, Volume: 45, Issue:3

    Lactobionic acid (LBA) has recently emerged as an important substance in various industries, such as cosmetics, foods, and pharmaceuticals. In this study, we developed a simple, efficient, and high-throughput method for screening LBA-producing microorganisms. First, an agar plate was prepared to isolate LBA-producing microorganisms by utilizing the property of LBA to solubilize colloidal calcium carbonate (CaCO

    Topics: Agar; Calcium Carbonate; Disaccharides; Phylogeny; RNA, Ribosomal, 16S

2022
Production of lactobionic acid at high salt concentrations by Acinetobacter halotolerans isolated from seaside soil.
    Bioprocess and biosystems engineering, 2022, Volume: 45, Issue:10

    A lactobionic acid (LBA)-producing bacterium isolated from seaside soils was identified as Acinetobacter halotolerans and designated as strain KRICT-1. We determined whether KRICT-1 can produce LBA at high salt concentrations. The KRICT-1 strain grew on a nutrient broth (NB) agar plate with up to 7.0% NaCl, indicating high NaCl tolerance, and 30 °C was the optimum growth temperature for LBA production. We produced LBA using the KRICT-1 strain in NB medium containing various concentrations of NaCl. While Pseudomonas taetrolens, an efficient LBA-producing bacterium, could produce LBA with up to 5.5% NaCl, the KRICT-1 strain could produce LBA at up to 7.0% NaCl and produced more LBA than P. taetrolens with over 5.5% NaCl. We produced LBA using NB medium containing 7.0% NaCl by batch fermentation of the KRICT-1 strain in a 5 L fermenter. The LBA production titer and productivity of the KRICT-1 strain were 32.1 g/L and 0.22 g/L/h, respectively, which were approximately 1.35- and 1.38-fold higher than those (23.7 g/L and 0.16 g/L/h) obtained from flask culture. Additionally, quinoprotein glucose dehydrogenase is an LBA-producing enzyme in A. halotolerans. We demonstrated that the A. halotolerans KRICT-1 strain is appropriate for LBA production at high salt concentrations.

    Topics: Acinetobacter; Agar; Bacteria; Culture Media; Disaccharides; Sodium Chloride; Soil

2022