agar and heliomycin

It was shown that a nutrient medium containing only salt components except agar-agar could be used for assay of the biological activity of heliomycin. The optimal results were observed on the medium containing ammonium chloride, urea, disubstituted sodium phosphate, glucose and agar-agar. The medium provided satisfactory growth of Bac. subtilis ATCC 6633 as the test culture and formation of clear inhibition growth zones of the sufficient size. The medium is standard and its use allows obtaining reproducible results in assay of the heliomycin biological activity.

Topics: Agar; Anti-Bacterial Agents; Bacillus subtilis; Biological Assay; Culture Media; Diffusion; Dose-Response Relationship, Drug; Polycyclic Compounds

A biological method for determination of geliomycin activity using agar diffusion is described. A nutrient medium containing Hottinger broth up to 35 mg per cent of amine nitrogen, 1.5 percent of agar-agar, tap water, pH 7.8 to 8.0 was used. Bacillus subtilis ATCC 6633 served as the test-culture. Geliomycin was dissolved in 0.1 N sodium hydroxide solution.

Topics: Agar; Anti-Bacterial Agents; Bacillus subtilis; Darkness; Diffusion; Dose-Response Relationship, Drug; Hydrogen-Ion Concentration; Lactones; Light; Ointments; Polycyclic Compounds; Solutions; Spectrophotometry