agar has been researched along with benzylaminopurine* in 1 studies
1 other study(ies) available for agar and benzylaminopurine
Article | Year |
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Production of withaferin A in shoot cultures of Withania somnifera.
Multiple shoot cultures of Withania somnifera were established from single shoot tip explants and their potential for the production of two principle withanolides, withaferin A and withanolide D was investigated. Shoot tips grown on MS medium supplemented with BA (1 mg l(-1)) induced 10.0 +/- 1.15 microshoots per explants and shoot cultures accumulated both withanolides (withaferin A = 0.04%, withanolide D = 0.06%). Supplementation of MSSM (solid) agar medium with 4% sucrose enhanced accumulation of both withaferin A (0.16%) and withanolide D (0.08%). Reduction of the agar concentration to 0.16% increased the number of microshoots induced per explant to 25.5. MSSM liquid medium containing 10% coconut milk favoured a maximum increase in biomass (27 fold); number of microshoots induced (37.6 +/- 1.45) as well as accumulation of withaferin A (0.14%). Topics: Adenine; Agar; Antineoplastic Agents, Phytogenic; Benzyl Compounds; Carbon; Culture Media; Culture Techniques; Ergosterol; Kinetin; Plant Extracts; Plant Growth Regulators; Plant Shoots; Purines; Solanaceae; Withanolides | 2001 |