agar has been researched along with 4-methylumbelliferyl-phosphate* in 1 studies
1 other study(ies) available for agar and 4-methylumbelliferyl-phosphate
Article | Year |
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[A simple and rapid method capable of detecting methicillin-resistant Staphylococcus aureus (MRSA) by single culture on an agar plate].
A novel simple agar plate capable of detecting methicillin-resistant Staphylococcus aureus (MRSA) by single culture was developed. The agar plate included 1) mannitol and a pH indicator, and 4-methylumbelliferyl phosphoric acid, both for identification of Staphylococcus aureus by its ability to digest mannitol and produce phosphatase; detection of the former by change in color of pH indicator and the latter by fluorescence of 4-methylumbelliferone, 2) oxacillin at a minimum concentration just to eliminate methicillin-sensitive Staphylococcus aureus (MSSA), and 3) phenylethyl alcohol to inhibit growth of most gram negative rods. The method was simple, sensitive, time-saving, and quite effective in the identification of MRSA directly from clinical specimens. Neither false positive nor false negative results were observed by the present method. Topics: Agar; Bacteriological Techniques; Culture Media; Fluorescence; Hydrogen-Ion Concentration; Hymecromone; Mannitol; Methicillin Resistance; Oxacillin; Staphylococcus aureus | 1993 |