aflatoxin-b and aflatoxicol

The aim was to evaluate the excretion of residues of aflatoxin B(1) (AFB(1)), aflatoxin M(1) (AFM(1)), aflatoxin B(2a) (AFB(2a)) and aflatoxicol (AFL) in eggs of laying Japanese quail fed rations with low levels of aflatoxin B(1) for 90 days. The quail were randomly assigned into four experimental groups and given prepared rations containing either 0 (controls), 25, 50 or 100 microg AFB(1) kg(-1) feed. Thirty-two eggs per treatment were collected on days 1-7, 10, 20, 30, 60 and 90 of the aflatoxin treatment period, and submitted to aflatoxin analysis by high-performance liquid chromatography. Average egg production and feed consumption were not affected ( p > 0.05) by AFB(1). Egg weight was significantly lower ( p<0.05) only for groups exposed to 100 microg AFB(1) kg(-1). Residues of aflatoxins were detected in eggs at levels that ranged from 0.01 to 0.08 microg kg(-1) (AFB(1)), 0.03-0.37 microg kg(-1) (AFM(1)), 0.01-1.03 microg kg(-1)(AFB(2a)) and 0.01-0.03 microg kg(-1) (AFL). Results indicate that the excretion of aflatoxin residues in quail eggs might occur at relatively low concentrations under conditions of long-term exposure of quail to low levels of AFB(1).

Topics: Aflatoxin B1; Aflatoxin M1; Aflatoxins; Animal Feed; Animals; Chromatography, High Pressure Liquid; Coturnix; Eggs; Food Contamination; Mycotoxins

Larvae of the navel orangeworm (NOW), Amyelois transitella (Walker), a major pest of almonds and pistachios, and the codling moth (CM), Cydia pomonella (L.), the principal pest of walnuts and pome fruits, are commonly found in tree nut kernels that can be contaminated with aflatoxin, a potent carcinogen. The ability of larvae of these insects to metabolize aflatoxin B1 (AFB1) was examined. A field strain of NOW produced three AFB1 biotransformation products, chiefly aflatoxicol (AFL), and minor amounts of aflatoxin B2a (AFB2a) and aflatoxin M1 (AFM1). With AFL as a substrate, NOW larvae produced AFB1 and aflatoxicol M1 (AFLM1). A lab strain of CM larvae produced no detectable levels of AFB1 biotransformation products in comparison to a field strain which produced trace amounts of only AFL. Neither NOW nor CM produced AFB1-8,9-epoxide (AFBO), the principal carcinogenic metabolite of AFB1. In comparison, metabolism of AFB1 by chicken liver yielded mainly AFL, whereas mouse liver produced mostly AFM1 at a rate eightfold greater than AFL. Mouse liver also produced AFBO. The relatively high production of AFL by NOW compared to CM may reflect an adaptation to detoxify AFB1. NOW larvae frequently inhabit environments highly contaminated with fungi and, hence, aflatoxin. Only low amounts, if any, of this mycotoxin occur in the chief CM hosts, walnuts, and pome fruits. Characterizations of enzymes and co-factors involved in biotransformation of AFB1 are discussed.

Topics: Aflatoxin B1; Aflatoxin M1; Aflatoxins; Animals; Chickens; Glutathione; Larva; Mice; Mice, Inbred C57BL; Molecular Structure; Moths; NAD; NADP

Aflatoxin analysis of 40 percutaneous needle liver biopsies in 27 children with protein-energy malnutrition and 13 children with miscellaneous liver disease in The Sudan is reported. Aflatoxins B1, B2 and aflatoxicol were detected in 5 of the 16 biopsies from kwashiorkor but in none of 11 biopsies from marasmus or marasmic kwashiorkor. Aflatoxins G1, G2 and M2 were detected in 5 of 12 children with chronic liver disease. A very high concentration of aflatoxicol was found in a breast-fed infant with neonatal hepatitis of unknown etiology.

Topics: Aflatoxin B1; Aflatoxins; Breast Feeding; Child, Preschool; Female; Humans; Infant; Kwashiorkor; Liver; Protein-Energy Malnutrition; Sudan

Effects of aflatoxin B1 and three of its metabolites on cellular immune response were assessed with an assay based on inhibition of tritiated thymidine uptake by phytohemeagglutinin stimulated lymphocytes. In this in vitro system aflatoxin B1 and aflatoxin Q1 were strongly inhibitory (more than 50% inhibition) at concentrations of 10 mu/ml, whereas aflatoxicol and aflatoxin B2 alpha exhibited little inhibition at 10 micrograms/ml and only 45 to 50% inhibition at 25 micrograms/ml. Contrasts with single degrees of freedom and orthogonal polynomial analysis revealed that the pair of aflatoxin B1 and aflatoxin Q1 differed linearly and quadratically from the pair of aflatoxin B2 alpha and aflatoxicol, but within each pair there were no differences. Limited data with aflatoxin M1 revealed that it was slightly more active than aflatoxicol in the assay, but minimal replication prevented rigorous statistical testing. It may be theorized that the moderate to strong inhibition of blastogenesis by aflatoxin B1 and its metabolites could inhibit T lymphocyte functions, such as killer, helper, effector, or other immune processes, and thus compromise the immunological surveillance mechanism.

Topics: Aflatoxin B1; Aflatoxin M1; Aflatoxins; Animals; Carcinogens; Cattle; Depression, Chemical; Female; Lymphocyte Activation; Lymphocytes; Phytohemagglutinins

1. Difference spectroscopy studies indicated that tetrahydrodeoxyaflatoxin B1 and aflatoxicol bind slightly to DNA, whereas aflatoxins B2a, G2a, G2 and aflatoxicol bind to bovine and porcine spleen DNAse II but aflatoxins B1, B2, G1 and tetrahydrodeoxyaflatoxin did not. 2. Kinetic studies showed that aflatoxins B1, G1 and B2 activated bovine and porcine spleen DNAse II while aflatoxins B2a, G2a and G2 had an inhibiting effect. 3. Dissociation constants for the enzyme: substrate-aflatoxin complexes (KAS) as well as the inhibition constants (Ki) were obtained from kinetic studies.

Topics: Aflatoxin B1; Aflatoxins; Animals; Carcinogens; Cattle; Endodeoxyribonucleases; Enzyme Activation; Hydrogen-Ion Concentration; Spleen; Swine

Topics: Aflatoxin B1; Aflatoxin M1; Aflatoxins; Chromatography, High Pressure Liquid; Humans; Methods