adrenocorticotropin-zinc and tyrosyl-tryptophyl-alanyl-tryptophyl-phenylalaninamide

The tendency of peptides to adsorb to surfaces can raise a concern in variety of analytical fields where the qualitative/quantitative measurement of low concentration analytes (ng/mL-pg/mL) is required. To demonstrate the importance of using the optimal glassware/plasticware, four doping relevant model peptides (GHRP 5, TB-500, Insulin Lispro, Synachten) were chosen and their recovery from various surfaces were evaluated. Our experiments showed that choosing expensive consumables with low-bind characteristics is not beneficial in all cases. A careful selection of the consumables based on the evaluation of the physico/chemical features of the peptide is recommended.

Topics: Adsorption; Animals; Chromatography, High Pressure Liquid; Cosyntropin; Doping in Sports; Glass; Humans; Insulin Lispro; Oligopeptides; Polypropylenes