adonifoline and hordenine

To investigate the alkaloids from Senecio scandens.. Compounds were isolated with silica gel and Sephadex LH-20 column chromatography and their structures were determined by spectral analysis and chemical evidence. The hepatic cytotoxicity of isolated compounds was tested by MTT method in vitro.. Six alkaloids were obtained and identified as adonifoline (1), 7-angeloylturneforcidine (2), hordenine (3), 1, 3, 6, 6-tetramethyl-5, 6, 7, 8-tetrahydro-isoquinolin-8-one (4), 4-(pyrrolidin-2-one) -phenyl acetic acid (5), (4-pyrrolidinophenyl) acetic acid (6).. Compound 6 is a new natural product, compounds 3, 4 were obtained from the genus Senecio for the first time, compounds 2, 5 were obtained from this plant for the first time. Compound 1 showed significant growth inhibitory effect against hepatocyte at 100 micromol x L(-1).

Topics: Acetic Acid; Alkaloids; Animals; Cell Proliferation; Cells, Cultured; Chromatography, High Pressure Liquid; Dextrans; Hepatocytes; Lactones; Mice; Mice, Inbred ICR; Pyrrolizidine Alkaloids; Senecio; Silica Gel; Tyramine