adenosine diphosphate has been researched along with tryptophan in 139 studies
Timeframe | Studies, this research(%) | All Research% |
---|---|---|
pre-1990 | 17 (12.23) | 18.7374 |
1990's | 50 (35.97) | 18.2507 |
2000's | 70 (50.36) | 29.6817 |
2010's | 2 (1.44) | 24.3611 |
2020's | 0 (0.00) | 2.80 |
Authors | Studies |
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Yoshino, H | 1 |
Kägi, JH; Mertens, ML; Nagayama, K; Vasák, M; Wüthrich, K | 1 |
Atkins, WM; Villafranca, JJ | 1 |
Hiratsuka, T | 1 |
France, RM; Grossman, SH; Mattheis, JR | 1 |
Aviram, M; Cogan, U; Mokady, S | 1 |
Deléage, G; Di Pietro, A; Divita, G; Gautheron, DC; Roux, B | 1 |
Harwalkar, VA; Stein, LA | 1 |
Brochon, JC; Desmadril, M; Mouawad, L; Perahia, D; Yon, JM | 1 |
Horowitz, PM; Mann, CJ; Mitchell, T; Nishimura, JS; Ybarra, J | 1 |
Nakatsuka, M; Nomura, J; Ohta, T; Satoh, K; Shibata, Y; Tanaka, T | 1 |
Cioni, P; Strambini, GB | 1 |
Arata, T; Inoue, A; Osafune, M; Tanii, I | 1 |
Steinberg, M; Tyson, PA | 1 |
Bagshaw, CR; Warriner, KE; Wells, C | 1 |
Bobyleva-Guarriero, V; Lardy, HA; Wehbie, RS | 1 |
Fasman, GD; Szent-Györgyi, AG; Werber, MM | 2 |
Jörnvall, H; Koberstein, R; Noack, K; Rasched, I; Sund, H; Witzemann, V | 1 |
Högenauer, G | 1 |
Hashimoto, T; Miyazawa, S; Sakurai, T; Shindo, Y | 1 |
Buckingham, S; Maurer, HM; Spielvogel, AR; Wolff, JA | 1 |
Kuczenski, RT; Suelter, CH | 1 |
Costa-Tiozzo, R; Gál, EM | 1 |
Vekshin, NL | 1 |
Buchberger, A; Bukau, B; McCarty, JS; Milkereit, P; Reinstein, J; Schröder, H; Theyssen, H; Virgallita, G | 1 |
Auzat, I; Garel, JR; Gawlita, E | 1 |
Blanchoin, L; Carlier, MF; Didry, D; Marchand, JB; Perelroizen, I | 1 |
Johnson, JL; Reinhart, GD | 2 |
Bryant, FR; Stole, E | 1 |
Grell, E; Senior, AE; Weber, J; Wilke-Mounts, S | 1 |
Divita, G; Elalaoui, A; Goody, RS; Imbach, JL; Maury, G | 1 |
Chatterjee, IB; Mukhopadhyay, CK | 1 |
Grell, E; Lee, RS; Senior, AE; Weber, J; Wilke-Mounts, S | 1 |
Di Pietro, A; Divita, G; Guillot, D; Reboud, AM; Reboud, JP; Sontag, B | 1 |
Cardemil, E; Encinas, MV; Goldie, H; Rojas, MC | 1 |
Brindle, KM; Fulton, AM; Williams, SP | 1 |
Di Pietro, A; Divita, G; Gautheron, DC; Goody, RS | 1 |
Mann, CJ; Mitchell, T; Nishimura, JS; Ybarra, J | 1 |
Kim, E; Motoki, M; Muhlrad, A; Reisler, E; Seguro, K | 1 |
Bobkova, E; Botstein, D; Doyle, TC; Miller, CJ; Reisler, E | 1 |
Bowman, C; Senior, AE; Weber, J | 1 |
Goody, RS; Simon, I; Zerial, M | 1 |
Cheung, CW; Mas, MT | 1 |
de Wit, JG; den Blaauwen, T; Driessen, AJ; Fekkes, P; Kuiper, W | 1 |
Branlant, G; Gabellieri, E; Rahuel-Clermont, S; Strambini, GB | 1 |
Gibbons, DL; Gorovits, BM; Hay, N; Hixson, JD; Horowitz, PM; Lund, P; Ybarra, J | 1 |
Bukau, B; Packschies, L; Reinstein, J; Schuster, HP; Theyssen, H | 1 |
Brandolin, G; Dianoux, AC; Fiore, C; Lauquin, GJ; Le Saux, A; Roux, P; Schwimmer, C; Trézéguet, V; Vignais, PV | 1 |
Ajtai, K; Burghardt, TP; Park, S | 2 |
Rosen, BP; Zhou, T | 1 |
Boam, DJ; Coggins, JR; Idziak, C; Kelly, SM; Krell, T; Lapthorn, AJ; Price, NC | 1 |
Slepenkov, SV; Witt, SN | 1 |
Löbau, S; Senior, AE; Weber, J | 1 |
Senior, AE; Weber, J | 1 |
Morii, H; Shimizu, T | 1 |
Allison, WS; Dou, C; Fortes, PA | 1 |
Kasturi, SR; Maity, H | 1 |
Nadanaciva, S; Senior, AE; Weber, J | 2 |
Isaev-Ivanov, VV; Lantsov, VA | 1 |
Burston, SG; Clarke, AR; Cliff, MJ; Hay, N; Kad, NM; Lund, PA; Webb, MR | 1 |
Berger, CL; Chrin, L; Rovner, AS; Yengo, CM | 1 |
Nadanaciva, S; Senior, AE; Weber, J; Wilke-Mounts, S | 1 |
De La Cruz, EM; Ostap, EM; Rosenfeld, SS; Sweeney, HL; Wells, AL | 1 |
Gimi, K; Gros, P; Lerner-Marmarosh, N; Senior, AE; Urbatsch, IL | 1 |
Deutscher, J; Di Pietro, A; Fieulaine, S; Galinier, A; Gonzalo, P; Jault, JM; Nessler, S | 1 |
Jang, SB; Peters, JW; Seefeldt, LC | 1 |
Digits, JA; Hedstrom, L | 1 |
Baitin, DM; Isaev-Ivanov, VV; Kozlov, MG; Kuramitsu, S; Lanzov, VA; Masui, R | 1 |
Andersen, J; Champagne, D; Francischetti, IM; Ribeiro, JM | 1 |
Cheung, HC; Jefferson, GM; Rosenfeld, SS; Stein, R; Wriggers, W; Xing, J | 1 |
Buchet, R; Hagemann, H; Marcillat, O; Vial, C | 1 |
Fujiwara, K; Hayakawa, K; Konishi, K; Martinez, HM; Morales, MF; Onishi, H; Tanokura, M | 1 |
Joel, PB; Sweeney, HL; Trybus, KM | 1 |
Estes, JE; Gershman, LC; Kinosian, HJ; Selden, LA | 1 |
De La Cruz, EM; Ostap, EM; Sweeney, HL; Wells, AL | 1 |
Bagshaw, CR; Málnási-Csizmadia, A; Woolley, RJ | 1 |
Reinhart, GD; Riley-Lovingshimer, MR | 2 |
Bagshaw, CR; Botchway, SW; Kovacs, M; Malnasi-Csizmadia, A; Woolley, RJ | 1 |
Ajtai, K; Burghardt, TP; Muhlrad, A; Peyser, YM | 1 |
Allison, WS; Dong, K; Ren, H | 1 |
Burghardt, TP; Park, S | 1 |
Geeves, MA; Nyitrai, M; Szent-Györgyi, AG | 1 |
Chu, JW; Qu, Q; Sharom, FJ | 1 |
Biswas, EE; Biswas, SB; Flowers, S | 1 |
Cardemil, E; Espinosa, V; Kettlun, AM; Valenzuela, MA; Zanocco, A | 1 |
Goldman, YE; Ostap, EM; Shaw, MA | 1 |
Ito, K; Sutoh, K; Suzuki, Y; Uyeda, TQ; Yamamoto, K | 1 |
Horii, T; Sekiguchi, M; Takahashi, M; Yamazaki, J | 1 |
Galinier, A; Granet, Y; Grangeasse, C; Jault, JM; Lavergne, JP; Nessler, S; Pompeo, F | 1 |
Li, CC; Song, C; Wang, Q; Yang, X | 1 |
Iizuka, R; Maruyama, T; Miki, K; Odaka, M; Shomura, Y; Yohda, M; Yoshida, T | 1 |
Bagshaw, CR; Conibear, PB; Floyd, DN; Kovács, M; Málnási-Csizmadia, A; Wakelin, S; Woolley, RJ | 1 |
Duffieux, F; Jacquet, E; Lallemand, JY; Masscheleyn, S; Oberkampf, M; Pamlard, O; Ramaen, O; Stoven, V | 1 |
Ding, H; Mukerji, I; Oliver, D | 1 |
Krishnamurthy, G; Smith, D; Tawa, G; Wang, Y; Young, KH | 1 |
Daiho, T; Danko, S; Suzuki, H; Yamasaki, K | 1 |
Campbell, ID; Clarkson, J; Harris, DA; Shu, JC; Yudkin, MD | 1 |
Tapley, TL; Vickery, LE | 1 |
Belknap, B; Ikebe, M; Ikebe, R; Inoue, A; Sato, O; White, HD | 1 |
Hayaishi, O | 1 |
Bryan, KE; Rubenstein, PA | 1 |
Barman, T; Candau, R; Iorga, B; Lionne, C; Travers, F | 1 |
Bagshaw, CR; Conibear, PB; Málnási-Csizmadia, A | 1 |
Corvest, V; Falson, P; Haraux, F; Mueller, DM; Sigalat, C; Venard, R | 1 |
Jang, SB; Jeong, MS | 1 |
Bujalowski, W; Jezewska, MJ; Lucius, AL | 1 |
Flachner, B; Gráczer, E; Osváth, S; Szilágyi, AN; Varga, A; Vas, M | 1 |
Berger, CL; Chrin, LR; Gaffney, DP; Robertson, CI | 1 |
Catalano, CE; Gaussier, H; Maluf, NK; Ortega, ME | 1 |
Benabdelhak, H; Holland, IB; Jenewein, S; Schmitt, L; Wiedenmann, A; Zaitseva, J | 1 |
Feng, Z; Qin, G; Xicheng, W | 1 |
Edamatsu, M; Mizuno, N; Morii, H; Ogawa, K; Shimizu, T; Shimizu, Y; Toyoshima, YY | 1 |
Kannan, N; Neuwald, AF | 1 |
Jefferson, GM; King, PH; Rosenfeld, SS; Xing, J | 1 |
Iizuka, R; Inobe, T; Ishii, N; Kuwajima, K; Maki, K; Takahashi, K; Yohda, M; Yoshida, T; Zako, T | 1 |
Bujalowski, W; Galletto, R; Jezewska, MJ; Maillard, R | 1 |
Bhattacharyya, D; Chakraborty, A; Datta, AK; Datta, R; Sen, B | 1 |
Bukau, B; Mayer, MP; Vogel, M | 1 |
Hiromasa, Y; Hu, L; Roche, TE | 1 |
Russell, PL; Sharom, FJ | 1 |
Bagshaw, CR; Geeves, MA; Gyimesi, M; Kintses, B; Málnási-Csizmadia, A; Pearson, DS; Zeng, W | 1 |
Bruckner, RC; Ferreira, P; Jorns, MS; Zhao, G | 1 |
Bulygin, VV; Milgrom, YM | 1 |
Jahn, W | 1 |
Herdendorf, TJ; Miziorko, HM | 1 |
Mao, HZ; Weber, J | 1 |
Hiromasa, Y; Roche, TE | 1 |
Leskovar, A; Reinstein, J | 1 |
Aittoniemi, J; Ashcroft, FM; de Wet, H; Flanagan, SE; Hattersley, AT; Lafond, M; Pearson, ER; Proks, P; Sansom, MS | 1 |
Conilleau, S; Iwai, S; Kurumizaka, H; Renodon-Cornière, A; Takahashi, M; Takizawa, Y; Tran, V | 1 |
Balakrishna, AM; Grüber, G; Hunke, C; Kumar, A; Manimekalai, MS; Sewald, N; Weigelt, S | 1 |
Herman, P; Lee, JC | 1 |
Levitsky, DI; Logvinova, DS; Markov, DI; Nikolaeva, OP; Sluchanko, NN; Ushakov, DS | 1 |
Gayathri, N; Keshava Prasad, TS; Kumar, M; Nalini, A; Padmanabhan, B; Srinivas Bharath, MM; Sunitha, B | 1 |
1 review(s) available for adenosine diphosphate and tryptophan
Article | Year |
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Engineering Dictyostelium discoideum myosin II for the introduction of site-specific fluorescence probes.
Topics: Adenosine Diphosphate; Animals; Dictyostelium; Fluorescent Dyes; Genes, Reporter; Green Fluorescent Proteins; Kinetics; Luminescent Proteins; Myosin Type II; Protein Engineering; Protozoan Proteins; Tryptophan | 2002 |
138 other study(ies) available for adenosine diphosphate and tryptophan
Article | Year |
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The role of tryptophanyl residues in heavy meromyosin as studied by chemical modification with 2-hydroxy-5-nitrobenzyl bromide.
Topics: 2-Hydroxy-5-nitrobenzyl Bromide; Adenosine Diphosphate; Adenosine Triphosphatases; Adenosine Triphosphate; Diphosphates; Macromolecular Substances; Magnesium; Manganese; Myosin Subfragments; Phosphates; Potassium Chloride; Protein Binding; Trypsin; Tryptophan | 1976 |
Creatine kinase. Nuclear magnetic resonance and fluorescence evidence for interaction of adenosine 5'-diphosphate with aromatic residue(s).
Topics: Adenosine Diphosphate; Animals; Binding Sites; Creatine Kinase; Fourier Analysis; Kinetics; Magnetic Resonance Spectroscopy; Phenylalanine; Protein Binding; Protein Conformation; Rabbits; Spectrometry, Fluorescence; Tryptophan; Tyrosine | 1979 |
Time-resolved fluorescence studies of tryptophan mutants of Escherichia coli glutamine synthetase: conformational analysis of intermediates and transition-state complexes.
Topics: Adenosine Diphosphate; Adenosine Triphosphate; Escherichia coli; Glutamate-Ammonia Ligase; Hydrogen-Ion Concentration; Kinetics; Mutagenesis, Site-Directed; Quantum Theory; Recombinant Proteins; Spectrometry, Fluorescence; Time Factors; Tryptophan | 1992 |
Spatial proximity of ATP-sensitive tryptophanyl residue(s) and Cys-697 in myosin ATPase.
Topics: Adenosine Diphosphate; Adenosine Triphosphate; Anilino Naphthalenesulfonates; Animals; Cysteine; Myosins; Rabbits; Spectrometry, Fluorescence; Sulfhydryl Reagents; Tryptophan | 1992 |
Heterogeneous flexibilities of the active site domains of homodimeric creatine kinase: effect of substrate.
Topics: Adenosine Diphosphate; Animals; Binding Sites; Creatine; Creatine Kinase; In Vitro Techniques; Ligands; Motion; Muscles; Rabbits; Spectrometry, Fluorescence; Tryptophan | 1992 |
Dietary tryptophan enhances platelet aggregation in rats.
Topics: Adenosine Diphosphate; Animals; Diet; Female; Hydroxyindoleacetic Acid; Lipids; Male; Platelet Aggregation; Rats; Serotonin; Tryptophan; Weaning | 1990 |
Intrinsic tryptophan fluorescence of Schizosaccharomyces pombe mitochondrial F1-ATPase. A powerful probe for phosphate and nucleotide interactions.
Topics: Acrylamide; Acrylamides; Adenosine Diphosphate; Adenosine Triphosphate; Guanidine; Guanidines; Iodides; Macromolecular Substances; Mitochondria; Protein Conformation; Proton-Translocating ATPases; Schizosaccharomyces; Spectrometry, Fluorescence; Tryptophan | 1991 |
Characterization of an actin flow artifact that occurs during the presteady state dissociation of actosubfragment-1.
Topics: Actins; Adenosine Diphosphate; Adenosine Triphosphate; Animals; False Positive Reactions; Fluorescence; Kinetics; Light; Myosins; Peptide Fragments; Phosphates; Rabbits; Scattering, Radiation; Swine; Tryptophan | 1991 |
The effects of ligands on the conformation of phosphoglycerate kinase: fluorescence anisotropy decay and theoretical interpretation.
Topics: Adenosine Diphosphate; Animals; Birefringence; Fluorescence Polarization; Glyceric Acids; Glycolysis; Horses; Isomerism; Kinetics; Ligands; Macromolecular Substances; Phosphoglycerate Kinase; Protein Conformation; Spectrophotometry, Ultraviolet; Temperature; Tryptophan | 1990 |
Intrinsic fluorescence of succinyl-CoA synthetase and four tryptophan mutants. Tryptophan 76 and tryptophan 248 of the beta-subunit are responsive to CoA binding.
Topics: Adenosine Diphosphate; Adenosine Triphosphate; Coenzyme A; Coenzyme A Ligases; Iodides; Mutation; Phenylalanine; Protein Binding; Spectrometry, Fluorescence; Succinate-CoA Ligases; Succinates; Tryptophan | 1990 |
Effect of some purine metabolites contained in oyster on platelet aggregation.
Topics: Adenosine Diphosphate; Adult; Animals; Blood Glucose; Diabetes Mellitus, Experimental; Humans; Male; Nucleosides; Nucleotides; Ostreidae; Platelet Aggregation; Rats; Rats, Inbred Strains; Tissue Extracts; Tryptophan | 1989 |
Dynamical structure of glutamate dehydrogenase as monitored by tryptophan phosphorescence. Signal transmission following binding of allosteric effectors.
Topics: Adenosine Diphosphate; Allosteric Site; Animals; Cattle; Glutamate Dehydrogenase; Guanosine Triphosphate; Luminescent Measurements; Macromolecular Substances; Oxygen; Protein Conformation; Tryptophan | 1989 |
ATPase characteristics of myosin from nematode Caenorhabditis elegans purified by an improved method. Formation of myosin-phosphate-ADP complex and ATP-induced fluorescence enhancement.
Topics: Actins; Actomyosin; Adenosine Diphosphate; Adenosine Triphosphatases; Adenosine Triphosphate; Animals; Electrophoresis, Polyacrylamide Gel; Light; Myosins; Nematoda; Scattering, Radiation; Spectrometry, Fluorescence; Tryptophan | 1985 |
Accessibility of tryptophan residues in Na,K-ATPase.
Topics: Acrylamide; Acrylamides; Adenosine Diphosphate; Animals; Chymotrypsin; Dogs; In Vitro Techniques; Ouabain; Potassium; Protein Conformation; Protein Denaturation; Sodium; Sodium-Potassium-Exchanging ATPase; Spectrometry, Fluorescence; Tryptophan | 1987 |
Fluorescence studies on the nucleotide- and Ca2+-binding domains of molluscan myosin.
Topics: Adenosine Diphosphate; Adenosine Triphosphate; Animals; Calcium; Electrophoresis, Polyacrylamide Gel; Mollusca; Myosin Subfragments; Myosins; Peptide Fragments; Protein Binding; Spectrometry, Fluorescence; Tryptophan | 1985 |
The role of malate in hormone-induced enhancement of mitochondrial respiration.
Topics: Adenosine Diphosphate; Angiotensin II; Animals; Epinephrine; Glucagon; Hormones; Malates; Male; Mitochondria, Liver; Norepinephrine; Oxygen Consumption; Rats; Rats, Inbred Strains; Tryptophan; Vasopressins | 1986 |
Fluorescence studies on the interaction between heavy meromyosin and magnesium ions. Effect of EDTA.
Topics: Adenosine Diphosphate; Adenosine Triphosphate; Binding Sites; Carbon Radioisotopes; Edetic Acid; Kinetics; Magnesium; Mathematics; Myosin Subfragments; Myosins; Protein Binding; Protein Conformation; Spectrometry, Fluorescence; Tryptophan | 1973 |
Fluorescence studies on heavy meromyosin-substrate interaction.
Topics: Adenosine Diphosphate; Adenosine Triphosphatases; Adenosine Triphosphate; Animals; Calcium; Chromatography, Gel; Ethylmaleimide; Hydrolysis; Iodides; Magnesium; Mathematics; Muscle Proteins; Myosin Subfragments; Myosins; Nucleotides; Organomercury Compounds; Osmolar Concentration; Protein Binding; Protein Conformation; Rabbits; Spectrometry, Fluorescence; Structure-Activity Relationship; Tryptophan | 1972 |
Studies of glutamate dehydrogenase: chemical modification and quantitative determination of tryptophan residues.
Topics: Adenosine Diphosphate; Amino Acid Sequence; Amino Acids; Animals; Benzyl Compounds; Binding Sites; Cattle; Chromatography, Gel; Chymotrypsin; Circular Dichroism; Dansyl Compounds; Glutamate Dehydrogenase; Guanine Nucleotides; Hydrogen-Ion Concentration; Kinetics; Liver; NAD; Oxidation-Reduction; Peptide Fragments; Phenols; Protein Binding; Protein Conformation; Spectrophotometry, Ultraviolet; Sulfhydryl Compounds; Tryptophan; Ultracentrifugation | 1974 |
Binding of UGA to wild type and suppressor tryptophan tRNA from E. coli.
Topics: Adenosine Diphosphate; Animals; Base Sequence; Binding Sites; Carbon Radioisotopes; Chromatography, Gel; Cytosine Nucleotides; Escherichia coli; Genetic Code; Mutation; Oligonucleotides; Phosphoric Diester Hydrolases; RNA, Bacterial; RNA, Transfer; Snakes; Suppression, Genetic; Transfer RNA Aminoacylation; Tritium; Tryptophan; Uracil Nucleotides; Venoms | 1974 |
The effect of tryptophan administration on fatty acid synthesis in the liver of the fasted normal rat.
Topics: Acetates; Adenosine Diphosphate; Adenosine Monophosphate; Adenosine Triphosphate; Alanine; Animals; Carbon Radioisotopes; Cholesterol; Chromatography, Thin Layer; Citric Acid Cycle; Fasting; Fatty Acids; Fatty Acids, Nonesterified; Glycerol; Intestine, Small; Lipids; Liver; Male; Phospholipids; Pyruvate Dehydrogenase Complex; Quinolines; Rats; Time Factors; Triglycerides; Tritium; Tryptophan; Water | 1974 |
"Impotent" platelets in albinos with prolonged bleeding times.
Topics: Adenosine Diphosphate; Adolescent; Albinism; Blood Cell Count; Blood Coagulation Disorders; Blood Coagulation Factors; Blood Coagulation Tests; Blood Platelet Disorders; Blood Platelets; Child; Collagen; Female; Humans; Lipoproteins; Male; Microscopy, Electron; Norepinephrine; Platelet Adhesiveness; Serotonin; Tryptophan | 1972 |
Interactions of Fructose 1,6-diphosphate, substrates, and monovalent cations with yeast pyruvate kinase monitored by changes in enzyme fluorescence.
Topics: Adenosine Diphosphate; Allosteric Regulation; Chemical Phenomena; Chemistry; Chlorides; Enzyme Activation; Fluorescence; Fluorometry; Fructose; Fructosephosphates; Hexosephosphates; Kinetics; Magnesium; Phosphoenolpyruvate; Potassium Chloride; Protein Binding; Protein Conformation; Pyruvate Kinase; Quaternary Ammonium Compounds; Saccharomyces; Temperature; Tryptophan; Ultracentrifugation | 1971 |
Synaptosomal uptake: the modifying effect of mitochondria.
Topics: Adenosine Diphosphate; Animals; Brain; Centrifugation, Density Gradient; Electron Transport Complex IV; Ficoll; Fumarate Hydratase; In Vitro Techniques; Kinetics; L-Lactate Dehydrogenase; Malates; Male; Mitochondria; Norepinephrine; Oligomycins; Oxygen Consumption; Pyruvates; Rats; Synaptosomes; Tryptophan | 1980 |
Luminescence studies on the conformational behavior of horse-liver alcohol dehydrogenase.
Topics: Adenosine; Adenosine Diphosphate; Alcohol Dehydrogenase; Alcohol Oxidoreductases; Animals; Energy Transfer; Ethanol; Horses; Liver; Luminescent Measurements; Mathematics; NAD; Protein Conformation; Tryptophan | 1984 |
Nucleotide-induced conformational changes in the ATPase and substrate binding domains of the DnaK chaperone provide evidence for interdomain communication.
Topics: Adenosine Diphosphate; Adenosine Triphosphatases; Adenosine Triphosphate; Animals; Binding Sites; Escherichia coli Proteins; Fluorescence; HSP70 Heat-Shock Proteins; Mice; Mice, Inbred BALB C; Peptide Fragments; Protein Conformation; Tryptophan | 1995 |
Slow ligand-induced transitions in the allosteric phosphofructokinase from Escherichia coli.
Topics: Adenosine Diphosphate; Adenylyl Imidodiphosphate; Allosteric Regulation; Allosteric Site; Crystallography, X-Ray; Escherichia coli; Fructosephosphates; Kinetics; Ligands; Phosphoenolpyruvate; Phosphofructokinase-1; Protein Conformation; Spectrometry, Fluorescence; Time Factors; Tryptophan | 1995 |
Interaction of profilin with G-actin and poly(L-proline).
Topics: Actins; Adenosine Diphosphate; Adenosine Triphosphate; Animals; Calcium; Cations, Divalent; Cattle; Contractile Proteins; Kinetics; Microfilament Proteins; Osmolar Concentration; Peptides; Profilins; Rabbits; Spectrometry, Fluorescence; Spleen; Structure-Activity Relationship; Tryptophan; Ultracentrifugation | 1994 |
Influence of MgADP on phosphofructokinase from Escherichia coli. Elucidation of coupling interactions with both substrates.
Topics: Adenosine Diphosphate; Adenosine Triphosphate; Allosteric Regulation; Binding Sites; Escherichia coli; Fructosephosphates; Kinetics; Ligands; Phosphofructokinase-1; Spectrometry, Fluorescence; Thermodynamics; Tryptophan | 1994 |
Influence of substrates and MgADP on the time-resolved intrinsic fluorescence of phosphofructokinase from Escherichia coli. Correlation of tryptophan dynamics to coupling entropy.
Topics: Adenosine Diphosphate; Adenosine Triphosphate; Escherichia coli; Fluorescence Polarization; Iodides; Kinetics; Phosphofructokinase-1; Spectrometry, Fluorescence; Thermodynamics; Tryptophan | 1994 |
Introduction of a tryptophan reporter group into loop 1 of the recA protein. Examination of the conformational states of the recA-ssDNA complex by fluorescence spectroscopy.
Topics: Adenosine Diphosphate; Adenosine Triphosphate; Amino Acid Sequence; Base Sequence; Binding Sites; DNA Primers; DNA, Single-Stranded; Histidine; Kinetics; Molecular Sequence Data; Mutagenesis, Site-Directed; Nucleic Acid Conformation; Protein Conformation; Rec A Recombinases; Recombinant Proteins; Spectrometry, Fluorescence; Tryptophan | 1994 |
Tryptophan-free Escherichia coli F1-ATPase.
Topics: Adenosine Diphosphate; Adenosine Triphosphate; Aurovertins; Azides; Base Sequence; Dicyclohexylcarbodiimide; Escherichia coli; Hydrogen-Ion Concentration; Molecular Sequence Data; Mutagenesis, Site-Directed; Mutation; Oxidative Phosphorylation; Proton-Translocating ATPases; Spectrometry, Fluorescence; Tryptophan | 1994 |
Intrinsic tryptophan fluorescence of bovine liver adenosine kinase, characterization of ligand binding sites and conformational changes.
Topics: Adenosine; Adenosine Diphosphate; Adenosine Kinase; Adenosine Monophosphate; Adenosine Triphosphate; Animals; Binding Sites; Cattle; Electrophoresis, Polyacrylamide Gel; Ligands; Liver; Magnesium Chloride; Molecular Weight; Protein Conformation; Spectrometry, Fluorescence; Tryptophan | 1994 |
NADPH-initiated cytochrome P450-mediated free metal ion-independent oxidative damage of microsomal proteins. Exclusive prevention by ascorbic acid.
Topics: Adenosine Diphosphate; Animals; Ascorbic Acid; Cytochrome P-450 Enzyme Inhibitors; Cytochrome P-450 Enzyme System; Guinea Pigs; Hydrolysis; Iron; Male; Microsomes, Liver; NADP; Oxidation-Reduction; Proteins; Tryptophan; Tyrosine | 1994 |
Specific placement of tryptophan in the catalytic sites of Escherichia coli F1-ATPase provides a direct probe of nucleotide binding: maximal ATP hydrolysis occurs with three sites occupied.
Topics: Adenosine Diphosphate; Adenosine Triphosphate; Adenylyl Imidodiphosphate; Amino Acid Sequence; Base Sequence; Binding Sites; Escherichia coli; Hydrolysis; Molecular Sequence Data; Mutagenesis, Site-Directed; Oligodeoxyribonucleotides; Proton-Translocating ATPases; Recombinant Proteins; Spectrometry, Fluorescence; Tryptophan | 1993 |
Intrinsic tryptophan fluorescence of rat liver elongation factor eEF-2 to monitor the interaction with guanylic and adenylic nucleotides and related conformational changes.
Topics: Adenine Nucleotides; Adenosine Diphosphate; Adenosine Triphosphate; Animals; Guanine Nucleotides; Guanosine Diphosphate; Guanosine Triphosphate; Kinetics; Mathematics; Peptide Elongation Factor 2; Peptide Elongation Factors; Protein Conformation; Rats; Spectrometry, Fluorescence; Tryptophan | 1993 |
Comparative steady-state fluorescence studies of cytosolic rat liver (GTP), Saccharomyces cerevisiae (ATP) and Escherichia coli (ATP) phospho enol pyruvate carboxykinases.
Topics: Acrylamide; Acrylamides; Adenosine Diphosphate; Adenosine Triphosphate; Animals; Binding Sites; Cytosol; Escherichia coli; Fluorescence; Guanosine Triphosphate; Liver; Manganese; Nucleotides; Phosphoenolpyruvate Carboxykinase (GTP); Protein Conformation; Rats; Saccharomyces cerevisiae; Sodium Iodide; Tryptophan | 1993 |
Estimation of the intracellular free ADP concentration by 19F NMR studies of fluorine-labeled yeast phosphoglycerate kinase in vivo.
Topics: Adenine Nucleotides; Adenosine Diphosphate; Cytosol; Diphosphoglyceric Acids; Enzyme Induction; Fluorine Radioisotopes; Glyceric Acids; Histocytochemistry; Hydrogen-Ion Concentration; Ligands; Magnetic Resonance Spectroscopy; Phosphoglycerate Kinase; Recombinant Proteins; Saccharomyces cerevisiae; Transformation, Genetic; Tryptophan | 1993 |
Structural mapping of catalytic site with respect to alpha-subunit and noncatalytic site in yeast mitochondrial F1-ATPase using fluorescence resonance energy transfer.
Topics: Adenosine Diphosphate; Adenosine Triphosphate; Binding Sites; Catalysis; Energy Transfer; Fluorescent Dyes; Guanosine Diphosphate; Mitochondria; Proton-Translocating ATPases; Schizosaccharomyces; Spectrometry, Fluorescence; Tryptophan | 1993 |
Sensitivity of Escherichia coli succinyl-CoA mutants at Trp beta 76 to clostripain and to trypsin. ADP and ATP protect against cleavage by clostripain at Arg beta 80.
Topics: Acyl Coenzyme A; Adenosine Diphosphate; Adenosine Triphosphate; Arginine; Base Sequence; Cysteine Endopeptidases; Electrophoresis, Polyacrylamide Gel; Escherichia coli; Hydrolysis; Molecular Sequence Data; Mutation; Oligodeoxyribonucleotides; Phosphorylation; Succinate-CoA Ligases; Trypsin; Tryptophan | 1993 |
Conformational changes in subdomain 2 of G-actin: fluorescence probing by dansyl ethylenediamine attached to Gln-41.
Topics: Actins; Adenosine Diphosphate; Adenosine Triphosphate; Animals; Binding Sites; Biophysical Phenomena; Biophysics; Biopolymers; Calcium; Dansyl Compounds; Energy Transfer; Ethylenediamines; Fluorescent Dyes; Glutamine; In Vitro Techniques; Magnesium; Muscle, Skeletal; Protein Conformation; Rabbits; Transglutaminases; Tryptophan | 1995 |
Mutational analysis of the role of hydrophobic residues in the 338-348 helix on actin in actomyosin interactions.
Topics: Actin Cytoskeleton; Actins; Actomyosin; Adenosine Diphosphate; Adenosine Triphosphate; Base Sequence; Circular Dichroism; Electrophoresis, Polyacrylamide Gel; Fluorescence; Kinetics; Molecular Sequence Data; Mutagenesis, Site-Directed; Myosin Subfragments; Myosins; Phalloidine; Protein Binding; Protein Structure, Secondary; Tryptophan; Yeasts | 1996 |
Specific tryptophan substitution in catalytic sites of Escherichia coli F1-ATPase allows differentiation between bound substrate ATP and product ADP in steady-state catalysis.
Topics: Adenosine Diphosphate; Adenosine Triphosphate; Adenylyl Imidodiphosphate; Base Sequence; Binding Sites; Catalysis; Escherichia coli; Fluorescent Dyes; Hydrolysis; Kinetics; Magnesium; Models, Biological; Molecular Sequence Data; Mutagenesis, Insertional; Oligodeoxyribonucleotides; Proton-Translocating ATPases; Spectrometry, Fluorescence; Substrate Specificity; Tryptophan | 1996 |
Kinetics of interaction of Rab5 and Rab7 with nucleotides and magnesium ions.
Topics: Adenosine Diphosphate; Adenosine Triphosphate; Base Sequence; Cell-Free System; DNA Primers; Escherichia coli; GTP Phosphohydrolases; GTP-Binding Proteins; Guanosine Diphosphate; Guanosine Triphosphate; Kinetics; Magnesium; Molecular Sequence Data; rab GTP-Binding Proteins; rab5 GTP-Binding Proteins; rab7 GTP-Binding Proteins; Recombinant Proteins; Spectrometry, Fluorescence; Tryptophan | 1996 |
Substrate-induced conformational changes in yeast 3-phosphoglycerate kinase monitored by fluorescence of single tryptophan probes.
Topics: Adenosine Diphosphate; Adenosine Monophosphate; Adenosine Triphosphate; Binding Sites; Catalysis; Diphosphoglyceric Acids; Fluorescence; Fluorescent Dyes; Glyceric Acids; Magnesium; Mutagenesis; Phosphoglycerate Kinase; Point Mutation; Protein Conformation; Protein Structure, Tertiary; Saccharomyces cerevisiae; Spectrometry, Fluorescence; Titrimetry; Tryptophan | 1996 |
Domain interactions of the peripheral preprotein Translocase subunit SecA.
Topics: Adenosine Diphosphate; Adenosine Triphosphatases; Adenylyl Imidodiphosphate; Amino Acid Sequence; Bacterial Proteins; Calorimetry, Differential Scanning; Escherichia coli; Escherichia coli Proteins; Membrane Transport Proteins; Models, Chemical; Mutagenesis, Site-Directed; Peptide Fragments; Recombinant Proteins; Restriction Mapping; Scattering, Radiation; SEC Translocation Channels; SecA Proteins; Sequence Deletion; Spectrometry, Fluorescence; Thermodynamics; Trypsin; Tryptophan | 1996 |
Effects of NAD+ binding on the luminescence of tryptophans 84 and 310 of glyceraldehyde-3-phosphate dehydrogenase from Bacillus stearothermophilus.
Topics: Adenosine Diphosphate; Adenosine Monophosphate; Fluorescence; Geobacillus stearothermophilus; Glyceraldehyde-3-Phosphate Dehydrogenases; Kinetics; Luminescent Measurements; Mutagenesis, Site-Directed; Mutation; NAD; Protein Conformation; Tryptophan | 1996 |
Intrinsic fluorescence studies of the chaperonin GroEL containing single Tyr --> Trp replacements reveal ligand-induced conformational changes.
Topics: Adenosine Diphosphate; Adenylyl Imidodiphosphate; Chaperonin 60; Magnesium; Mutagenesis, Site-Directed; Potassium; Protein Conformation; Spectrometry, Fluorescence; Tryptophan; Tyrosine | 1996 |
The second step of ATP binding to DnaK induces peptide release.
Topics: Adenosine Diphosphate; Adenosine Triphosphate; Escherichia coli Proteins; Fluorescent Dyes; HSP70 Heat-Shock Proteins; Hydrolysis; Molecular Conformation; Peptides; Protein Binding; Tryptophan | 1996 |
Conformational changes of the yeast mitochondrial adenosine diphosphate/adenosine triphosphate carrier studied through its intrinsic fluorescence. 2. Assignment of tryptophanyl residues of the carrier to the responses to specific ligands.
Topics: Adenosine Diphosphate; Atractyloside; Binding Sites; Bongkrekic Acid; Kinetics; Mitochondria; Mitochondrial ADP, ATP Translocases; Models, Structural; Mutagenesis, Site-Directed; Point Mutation; Protein Conformation; Protein Structure, Secondary; Recombinant Proteins; Saccharomyces cerevisiae; Spectrometry, Fluorescence; Tryptophan | 1996 |
Mechanism for coupling free energy in ATPase to the myosin active site.
Topics: Adenosine Diphosphate; Adenosine Triphosphate; Animals; Binding Sites; Kinetics; Muscle, Skeletal; Myosin Subfragments; Myosins; Rabbits; Spectrometry, Fluorescence; Thermodynamics; Tryptophan | 1997 |
Tryptophan fluorescence reports nucleotide-induced conformational changes in a domain of the ArsA ATPase.
Topics: Adenosine Diphosphate; Adenosine Triphosphatases; Adenosine Triphosphate; Amino Acid Sequence; Arsenite Transporting ATPases; Arsenites; Consensus Sequence; Escherichia coli; Hydrolysis; Ion Pumps; Membrane Proteins; Molecular Sequence Data; Multienzyme Complexes; Operon; Oxidation-Reduction; Protein Conformation; Spectrometry, Fluorescence; Tryptophan | 1997 |
The interaction of shikimate kinase from Erwinia chrystanthemi with substrates.
Topics: Adenosine Diphosphate; Binding Sites; Dickeya chrysanthemi; Kinetics; Phosphotransferases (Alcohol Group Acceptor); Shikimic Acid; Spectrometry, Fluorescence; Tryptophan | 1997 |
Kinetics of the reactions of the Escherichia coli molecular chaperone DnaK with ATP: evidence that a three-step reaction precedes ATP hydrolysis.
Topics: Adenosine Diphosphate; Adenosine Triphosphate; Bacterial Proteins; Escherichia coli; Escherichia coli Proteins; HSP70 Heat-Shock Proteins; Hydrolysis; Kinetics; Models, Chemical; Molecular Chaperones; Spectrometry, Fluorescence; Thermodynamics; Tryptophan | 1998 |
Catalytic site nucleotide binding and hydrolysis in F1F0-ATP synthase.
Topics: Adenosine Diphosphate; Adenosine Triphosphate; Binding Sites; Catalysis; Hydrolysis; Kinetics; Magnesium; Mutagenesis, Site-Directed; Proteolipids; Proton Pumps; Proton-Translocating ATPases; Spectrometry, Fluorescence; Tryptophan; Tyrosine | 1998 |
Effects of the inhibitors azide, dicyclohexylcarbodiimide, and aurovertin on nucleotide binding to the three F1-ATPase catalytic sites measured using specific tryptophan probes.
Topics: Adenosine Diphosphate; Adenosine Triphosphate; Aurovertins; Catalytic Domain; Dicyclohexylcarbodiimide; Enzyme Inhibitors; Molecular Probes; Protein Binding; Proton-Translocating ATPases; Sodium Azide; Spectrometry, Fluorescence; Tryptophan | 1998 |
Spectroscopic studies of the ncd motor domain.ADP complex: CD spectrum of ADP induced by binding to the motor domain of ncd.
Topics: Adenosine Diphosphate; Animals; Circular Dichroism; Creatine Kinase; Drosophila melanogaster; Drosophila Proteins; Fluorescent Dyes; Kinesins; Macromolecular Substances; Models, Molecular; Protein Binding; Protein Structure, Tertiary; Spectrometry, Fluorescence; Spectrophotometry, Ultraviolet; Tryptophan; Tyrosine | 1998 |
The alpha 3(beta Y341W)3 gamma subcomplex of the F1-ATPase from the thermophilic Bacillus PS3 fails to dissociate ADP when MgATP is hydrolyzed at a single catalytic site and attains maximal velocity when three catalytic sites are saturated with MgATP.
Topics: Adenosine Diphosphate; Adenosine Triphosphate; Animals; Azides; Bacillus; Binding Sites; Catalysis; Cattle; Cross-Linking Reagents; Detergents; Dimethylamines; Fluorescence Polarization; Hydrolysis; Kinetics; Mutagenesis, Site-Directed; Proton-Translocating ATPases; Spectrometry, Fluorescence; Tryptophan; Tyrosine | 1998 |
Inhibition of myosin ATPase by metal fluoride complexes.
Topics: Acrylamide; Actins; Adenosine Diphosphate; Binding Sites; Circular Dichroism; Enzyme Inhibitors; Fluorescence; Fluorides; Gallium; Magnesium Compounds; Myosins; Tryptophan | 1999 |
Interaction of bis(1-anilino-8-naphthalenesulfonate) with yeast hexokinase: a steady-state fluorescence study.
Topics: Adenosine Diphosphate; Anilino Naphthalenesulfonates; Energy Transfer; Fluorescence; Fluorescent Dyes; Glucose; Hexokinase; Tryptophan; Yeasts | 1998 |
The role of beta-Arg-182, an essential catalytic site residue in Escherichia coli F1-ATPase.
Topics: Adenosine Diphosphate; Adenosine Triphosphate; Aluminum; Amino Acid Substitution; Arginine; Binding Sites; Catalysis; Escherichia coli; Fluorine; Glutamine; Lysine; Mutagenesis, Site-Directed; Proton-Translocating ATPases; Spectrometry, Fluorescence; Tryptophan | 1999 |
[Conformational changes in the bacterial RecA protein upon its interaction with ligands: differential spectrofluorometry of tyrosine and tryptophan protein residues].
Topics: Adenosine Diphosphate; Adenosine Triphosphate; DNA, Single-Stranded; Escherichia coli; Ligands; Protein Binding; Protein Conformation; Rec A Recombinases; Spectrometry, Fluorescence; Tryptophan; Tyrosine | 1999 |
A kinetic analysis of the nucleotide-induced allosteric transitions of GroEL.
Topics: Adenosine Diphosphate; Adenosine Triphosphate; Allosteric Regulation; Amino Acid Substitution; Binding, Competitive; Chaperonin 60; Escherichia coli; Fluorescence; Fluorometry; Hydrolysis; Kinetics; Models, Chemical; Nucleotides; Phosphates; Protein Conformation; Thermodynamics; Tryptophan; Tyrosine | 1999 |
Intrinsic tryptophan fluorescence identifies specific conformational changes at the actomyosin interface upon actin binding and ADP release.
Topics: Actins; Actomyosin; Adenosine Diphosphate; Adenosine Triphosphate; Animals; Binding Sites; In Vitro Techniques; Microscopy, Electron; Models, Molecular; Molecular Motor Proteins; Muscle, Smooth; Protein Conformation; Protein Engineering; Recombinant Proteins; Spectrometry, Fluorescence; Tryptophan | 1999 |
Importance of F1-ATPase residue alpha-Arg-376 for catalytic transition state stabilization.
Topics: Adenosine Diphosphate; Adenosine Triphosphate; Aluminum; Arginine; Binding Sites; Catalysis; Cysteine; Enzyme Stability; Escherichia coli; Fluorine; Glutamine; Lysine; Models, Molecular; Mutagenesis, Site-Directed; Proton-Translocating ATPases; Spectrometry, Fluorescence; Tryptophan; Tyrosine | 1999 |
The kinetic mechanism of myosin V.
Topics: Actin Cytoskeleton; Actins; Actomyosin; Adenosine Diphosphate; Animals; Calmodulin-Binding Proteins; Chickens; Fluorescence; Kinetics; Myosin Type V; Myosins; Nerve Tissue Proteins; Phosphates; Rabbits; Tryptophan | 1999 |
Large scale purification of detergent-soluble P-glycoprotein from Pichia pastoris cells and characterization of nucleotide binding properties of wild-type, Walker A, and Walker B mutant proteins.
Topics: Adenosine Diphosphate; Adenosine Triphosphate; Animals; ATP Binding Cassette Transporter, Subfamily B; ATP-Binding Cassette Transporters; Chromatography; Dose-Response Relationship, Drug; Electrophoresis, Polyacrylamide Gel; Fermentation; Fluorescent Dyes; Kinetics; Mice; Microsomes; Mutagenesis; Pichia; Protein Binding; Spectrometry, Fluorescence; Tryptophan | 1999 |
The HPr kinase from Bacillus subtilis is a homo-oligomeric enzyme which exhibits strong positive cooperativity for nucleotide and fructose 1,6-bisphosphate binding.
Topics: Adenosine Diphosphate; Adenosine Triphosphate; Bacillus subtilis; Bacterial Proteins; Binding, Competitive; Dose-Response Relationship, Drug; Fructosediphosphates; Iodides; Kinetics; Nucleotides; Phosphorylation; Protein Binding; Protein Conformation; Protein Serine-Threonine Kinases; Spectrometry, Fluorescence; Tryptophan; Ultracentrifugation | 2000 |
Modulating the midpoint potential of the [4Fe-4S] cluster of the nitrogenase Fe protein.
Topics: Adenosine Diphosphate; Adenosine Triphosphate; Amino Acid Substitution; Bacterial Proteins; Crystallography, X-Ray; Iron-Sulfur Proteins; Models, Molecular; Nitrogenase; Oxidoreductases; Phenylalanine; Tryptophan | 2000 |
Drug selectivity is determined by coupling across the NAD+ site of IMP dehydrogenase.
Topics: Adenosine Diphosphate; Animals; Cricetinae; Cricetulus; Drug Resistance; Drug Synergism; Enzyme Inhibitors; Humans; IMP Dehydrogenase; Mutagenesis, Site-Directed; Mycophenolic Acid; NAD; Ribavirin; Spectrometry, Fluorescence; Tritrichomonas foetus; Tryptophan | 2000 |
Fluorescence and excitation Escherichia coli RecA protein spectra analyzed separately for tyrosine and tryptophan residues.
Topics: Adenosine Diphosphate; Adenosine Triphosphate; DNA, Bacterial; DNA, Single-Stranded; Escherichia coli; Ligands; Macromolecular Substances; Magnesium; Protein Conformation; Rec A Recombinases; Spectrometry, Fluorescence; Spectrophotometry; Tryptophan; Tyrosine | 2000 |
Purification, cloning, expression, and mechanism of action of a novel platelet aggregation inhibitor from the salivary gland of the blood-sucking bug, Rhodnius prolixus.
Topics: Adenosine Diphosphate; Amino Acid Sequence; Animals; Arachidonic Acid; Blood Platelets; Calcium; Cattle; Cell Adhesion; Cell Aggregation; Chromatography, High Pressure Liquid; Chromatography, Ion Exchange; Cloning, Molecular; Collagen; Crotalid Venoms; Cyclic AMP; DNA, Complementary; Dose-Response Relationship, Drug; Humans; Insect Proteins; Lectins, C-Type; Molecular Sequence Data; Platelet Aggregation Inhibitors; Rabbits; Rhodnius; Salivary Glands; Salivary Proteins and Peptides; Sequence Homology, Amino Acid; Thromboxane A2; Time Factors; Tryptophan | 2000 |
Kinesin has three nucleotide-dependent conformations. Implications for strain-dependent release.
Topics: Adenosine Diphosphate; Adenosine Triphosphatases; Binding Sites; Catalytic Domain; Computer Simulation; Crystallography, X-Ray; Humans; Kinesins; Kinetics; Microtubules; Models, Biological; Models, Molecular; Mutagenesis, Site-Directed; Nucleotides; Protein Conformation; Protein Structure, Tertiary; Spectrometry, Fluorescence; Time Factors; Tryptophan | 2000 |
Magnesium-adenosine diphosphate binding sites in wild-type creatine kinase and in mutants: role of aromatic residues probed by Raman and infrared spectroscopies.
Topics: Adenosine Diphosphate; Amino Acid Substitution; Animals; Binding Sites; Creatine Kinase; Isoenzymes; Magnesium; Muscle, Skeletal; Mutagenesis, Site-Directed; Rabbits; Solutions; Spectrometry, Fluorescence; Spectrophotometry, Infrared; Spectrum Analysis, Raman; Tryptophan; Tyrosine | 2000 |
New probes of the F1-ATPase catalytic transition state reveal that two of the three catalytic sites can assume a transition state conformation simultaneously.
Topics: Adenosine Diphosphate; Arginine; Binding Sites; Catalysis; Drug Synergism; Enzyme Activation; Enzyme Inhibitors; Escherichia coli; Glutamine; Inosine Diphosphate; Lysine; Mutagenesis, Site-Directed; Protein Conformation; Proton-Translocating ATPases; Scandium; Spectrometry, Fluorescence; Titrimetry; Tryptophan; Tyrosine | 2000 |
On the tryptophan residue of smooth muscle myosin that responds to binding of nucleotide.
Topics: Adenosine Diphosphate; Adenosine Triphosphate; Animals; Base Sequence; Binding Sites; DNA Primers; Muscle, Smooth; Mutagenesis; Myosins; Rabbits; Recombinant Proteins; Spectrometry, Fluorescence; Tryptophan | 2000 |
Two conserved lysines at the 50/20-kDa junction of myosin are necessary for triggering actin activation.
Topics: Actins; Adenosine Diphosphate; Adenosine Triphosphatases; Adenosine Triphosphate; Animals; Chickens; Conserved Sequence; In Vitro Techniques; Lysine; Molecular Weight; Mutation; Myosin Subfragments; Myosins; Protein Hydrolysates; Tryptophan | 2001 |
Interdependence of profilin, cation, and nucleotide binding to vertebrate non-muscle actin.
Topics: Actins; Adenosine Diphosphate; Adenosine Triphosphate; Animals; Binding Sites; Cations, Divalent; Cattle; Contractile Proteins; Ethenoadenosine Triphosphate; Kinetics; Magnesium; Microfilament Proteins; Models, Chemical; Profilins; Protein Denaturation; Protein Isoforms; Spectrometry, Fluorescence; Thermodynamics; Tryptophan | 2000 |
Actin and light chain isoform dependence of myosin V kinetics.
Topics: Actins; Adenosine Diphosphate; Adenosine Triphosphate; Amino Acid Motifs; Animals; Calmodulin-Binding Proteins; Chickens; Kinetics; Muscle, Skeletal; Myosin Light Chains; Myosin Type V; Myosins; Nerve Tissue Proteins; ortho-Aminobenzoates; Protein Binding; Protein Isoforms; Rabbits; Spectrometry, Fluorescence; Tryptophan | 2000 |
Resolution of conformational states of Dictyostelium myosin II motor domain using tryptophan (W501) mutants: implications for the open-closed transition identified by crystallography.
Topics: Adenosine Diphosphate; Adenosine Triphosphate; Adenylyl Imidodiphosphate; Aluminum Compounds; Animals; Binding Sites; Crystallography, X-Ray; Dictyostelium; Fluorides; Hydrolysis; Kinetics; Molecular Motor Proteins; Mutagenesis, Site-Directed; Myosins; Protein Conformation; Protein Structure, Tertiary; Spectrometry, Fluorescence; Tryptophan | 2000 |
Equilibrium binding studies of a tryptophan-shifted mutant of phosphofructokinase from Bacillus stearothermophilus.
Topics: Adenosine Diphosphate; Adenosine Triphosphate; Allosteric Regulation; Binding, Competitive; Enzyme Inhibitors; Geobacillus stearothermophilus; Kinetics; Mutagenesis, Site-Directed; Phenylalanine; Phosphoenolpyruvate; Phosphofructokinase-1; Protein Binding; Protein Conformation; Temperature; Thermodynamics; Tryptophan | 2001 |
The dynamics of the relay loop tryptophan residue in the Dictyostelium myosin motor domain and the origin of spectroscopic signals.
Topics: Adenosine Diphosphate; Adenosine Triphosphate; Animals; Anisotropy; Cloning, Molecular; Dictyostelium; Kinetics; Models, Chemical; Myosins; Phenylalanine; Protein Structure, Tertiary; Spectrometry, Fluorescence; Spectrophotometry; Time Factors; Tryptophan | 2001 |
Effect of ionic strength on the conformation of myosin subfragment 1-nucleotide complexes.
Topics: Adenosine Diphosphate; Adenosine Triphosphatases; Adenosine Triphosphate; Alkylation; Animals; Circular Dichroism; Cysteine; Lysine; Muscle, Skeletal; Myosin Subfragments; Nucleotides; Osmolar Concentration; Potassium; Protein Binding; Protein Conformation; Rabbits; Time Factors; Trinitrobenzenes; Tryptophan; Vanadates | 2001 |
The fluorescence spectrum of the introduced tryptophans in the alpha 3(beta F155W)3gamma subcomplex of the F1-ATPase from the thermophilic Bacillus PS3 cannot be used to distinguish between the number of nucleoside di- and triphosphates bound to catalytic
Topics: Adenosine Diphosphate; Adenosine Triphosphate; Adenylyl Imidodiphosphate; Bacillus; Catalytic Domain; Mutation; Protein Subunits; Proton-Translocating ATPases; Spectrometry, Fluorescence; Tryptophan | 2002 |
Tyrosine mediated tryptophan ATP sensitivity in skeletal myosin.
Topics: Adenosine Diphosphate; Adenosine Triphosphate; Animals; Models, Chemical; Muscle, Skeletal; Myosin Subfragments; Protein Conformation; Rabbits; Spectrometry, Fluorescence; Spectrophotometry; Tryptophan; Tyrosine | 2002 |
A kinetic model of the co-operative binding of calcium and ADP to scallop (Argopecten irradians) heavy meromyosin.
Topics: Adenosine Diphosphate; Adenosine Triphosphate; Animals; Ca(2+) Mg(2+)-ATPase; Calcium; In Vitro Techniques; Kinetics; Models, Biological; Mollusca; Myosin Subfragments; ortho-Aminobenzoates; Protein Binding; Protein Conformation; Spectrometry, Fluorescence; Tryptophan | 2002 |
Transition state P-glycoprotein binds drugs and modulators with unchanged affinity, suggesting a concerted transport mechanism.
Topics: Adenosine Diphosphate; Anilino Naphthalenesulfonates; Animals; ATP Binding Cassette Transporter, Subfamily B, Member 1; Azides; Benzimidazoles; Biological Transport; CHO Cells; Cobalt; Cricetinae; Dihydropyridines; Doxorubicin; Magnesium; Pharmaceutical Preparations; Photoaffinity Labels; Protein Binding; Spectrometry, Fluorescence; Tryptophan; Vanadates; Vinblastine | 2003 |
Conformational dynamics of DnaB helicase upon DNA and nucleotide binding: analysis by intrinsic tryptophan fluorescence quenching.
Topics: Adenosine Diphosphate; Adenosine Triphosphate; Amino Acid Sequence; Bacterial Proteins; Binding Sites; DNA Helicases; DNA-Binding Proteins; DnaB Helicases; Enzyme Activation; Escherichia coli Proteins; Models, Molecular; Molecular Sequence Data; Mutagenesis, Site-Directed; Oligonucleotides; Potassium Iodide; Protein Conformation; Protein Structure, Tertiary; Recombinant Proteins; Sequence Homology, Amino Acid; Spectrometry, Fluorescence; Titrimetry; Tryptophan | 2003 |
Differences in nucleotide-binding site of isoapyrases deduced from tryptophan fluorescence.
Topics: Acrylamide; Adenosine Diphosphate; Adenosine Triphosphate; Apyrase; Binding Sites; Cesium; Iodides; Isoenzymes; Kinetics; Nucleotides; Photobleaching; Solanum tuberosum; Spectrometry, Fluorescence; Substrate Specificity; Tryptophan | 2003 |
Mechanism of inhibition of skeletal muscle actomyosin by N-benzyl-p-toluenesulfonamide.
Topics: Actins; Adenosine Diphosphate; Adenosine Triphosphate; Animals; Binding Sites; Enzyme Inhibitors; Hydrolysis; In Vitro Techniques; Kinetics; Models, Biological; Muscle Contraction; Muscle, Skeletal; Myosin Subfragments; Phosphates; Pyrenes; Rabbits; Spectrometry, Fluorescence; Sulfonamides; Toluene; Tryptophan | 2003 |
Requirement of domain-domain interaction for conformational change and functional ATP hydrolysis in myosin.
Topics: Actins; Adenosine Diphosphate; Adenosine Triphosphatases; Adenosine Triphosphate; Animals; Cell Division; Cell Movement; Dictyostelium; Fluorescence; Fluorescence Resonance Energy Transfer; Hydrolysis; Mutagenesis, Site-Directed; Myosins; Phenotype; Protein Structure, Tertiary; Tryptophan | 2003 |
Regulation of RecA protein binding to DNA by opposing effects of ATP and ADP on inter-domain contacts: analysis by urea-induced unfolding of wild-type and C-terminal truncated RecA.
Topics: Adenosine Diphosphate; Adenosine Triphosphate; Circular Dichroism; DNA; DNA, Bacterial; DNA, Single-Stranded; Escherichia coli; Ligands; Models, Molecular; Nucleic Acid Conformation; Protein Binding; Protein Conformation; Protein Folding; Protein Structure, Tertiary; Rec A Recombinases; Recombination, Genetic; Spectrometry, Fluorescence; Tryptophan; Urea | 2003 |
Regulation and mutational analysis of the HPr kinase/phosphorylase from Bacillus subtilis.
Topics: Adenosine Diphosphate; Adenosine Triphosphate; Amino Acid Motifs; Amino Acid Sequence; Bacillus subtilis; Binding Sites; Fluorescence Resonance Energy Transfer; Fructosediphosphates; Kinetics; Magnesium; Models, Molecular; Molecular Sequence Data; Mutagenesis, Site-Directed; Phosphates; Phosphoprotein Phosphatases; Phosphorylation; Protein Serine-Threonine Kinases; Recombinant Proteins; Tryptophan | 2003 |
D1 ring is stable and nucleotide-independent, whereas D2 ring undergoes major conformational changes during the ATPase cycle of p97-VCP.
Topics: Adenosine Diphosphate; Adenosine Triphosphatases; Adenosine Triphosphate; Binding Sites; Blotting, Western; Cell Cycle Proteins; Chromatography, Gel; Chromatography, High Pressure Liquid; Cloning, Molecular; Electrophoresis, Polyacrylamide Gel; Hydrolysis; Models, Biological; Models, Genetic; Molecular Chaperones; Mutagenesis, Site-Directed; Mutation; Nucleotides; Protein Conformation; Protein Structure, Tertiary; Recombinant Fusion Proteins; Spectrometry, Fluorescence; Time Factors; Tryptophan; Valosin Containing Protein | 2003 |
ATP binding is critical for the conformational change from an open to closed state in archaeal group II chaperonin.
Topics: Adenosine Diphosphate; Adenosine Triphosphate; Ammonium Sulfate; Bacterial Proteins; Chaperonins; Electrophoresis, Polyacrylamide Gel; Endopeptidases; Escherichia coli; Fluorescence; Hydrolysis; Mutagenesis, Site-Directed; Potassium; Protein Conformation; Protein Folding; Spectrometry, Fluorescence; Structure-Activity Relationship; Thermococcus; Tryptophan | 2003 |
Biochemical characterization and NMR studies of the nucleotide-binding domain 1 of multidrug-resistance-associated protein 1: evidence for interaction between ATP and Trp653.
Topics: Adenosine Diphosphate; Adenosine Triphosphatases; Adenosine Triphosphate; Amino Acid Sequence; Binding Sites; HL-60 Cells; Humans; Magnesium; Molecular Sequence Data; Multidrug Resistance-Associated Proteins; Nuclear Magnetic Resonance, Biomolecular; Protein Structure, Tertiary; Recombinant Proteins; Sequence Alignment; Spectrometry, Fluorescence; Tryptophan | 2003 |
Nucleotide and phospholipid-dependent control of PPXD and C-domain association for SecA ATPase.
Topics: Adenosine Diphosphate; Adenosine Triphosphatases; Amino Acid Substitution; Anilino Naphthalenesulfonates; Bacillus subtilis; Bacterial Proteins; Cysteine; Escherichia coli Proteins; Fluorescence Resonance Energy Transfer; Membrane Transport Proteins; Models, Molecular; Phospholipids; Protein Structure, Tertiary; Recombinant Proteins; SEC Translocation Channels; SecA Proteins; Temperature; Tryptophan | 2003 |
Mutation of cysteine 214 in Gi1 alpha subunit abolishes its endogenous GTPase activity.
Topics: Adenosine Diphosphate; Adenylyl Cyclases; Alanine; Amino Acid Substitution; Animals; CHO Cells; Colforsin; Cricetinae; Cyclic AMP; Cysteine; GTP-Binding Protein alpha Subunits, Gi-Go; Guanosine Triphosphate; Hydrolysis; Kinetics; Mutation; Pertussis Toxin; Protein Binding; Protein Subunits; RGS Proteins; Thermodynamics; Trypsin; Tryptophan | 2004 |
Distinct natures of beryllium fluoride-bound, aluminum fluoride-bound, and magnesium fluoride-bound stable analogues of an ADP-insensitive phosphoenzyme intermediate of sarcoplasmic reticulum Ca2+-ATPase: changes in catalytic and transport sites during ph
Topics: Adenosine Diphosphate; Adenosine Monophosphate; Aluminum Compounds; Animals; Beryllium; Biochemical Phenomena; Biochemistry; Calcimycin; Calcium; Calcium-Transporting ATPases; Catalytic Domain; Cell Membrane; Chelating Agents; Crystallography, X-Ray; Cytoplasm; Dose-Response Relationship, Drug; Fluorides; Hydrogen-Ion Concentration; Hydrolysis; Magnesium Compounds; Models, Chemical; Muscle, Skeletal; Phosphorylation; Protein Binding; Protein Conformation; Protein Structure, Tertiary; Rabbits; Sarcoplasmic Reticulum; Sarcoplasmic Reticulum Calcium-Transporting ATPases; Spectrometry, Fluorescence; Thapsigargin; Time Factors; Tryptophan; Vanadates | 2004 |
Fluorescence and kinetic analysis of the SpoIIAB phosphorylation reaction, a key regulator of sporulation in Bacillus subtilis.
Topics: Adenosine Diphosphate; Adenosine Triphosphate; Bacillus subtilis; Bacterial Proteins; Binding Sites; Fluorescence Polarization; Kinetics; Ligands; Mutagenesis, Site-Directed; Phenylalanine; Phosphates; Phosphorylation; Spectrometry, Fluorescence; Spores, Bacterial; Tryptophan | 2004 |
Preferential substrate binding orientation by the molecular chaperone HscA.
Topics: Adenosine Diphosphate; Adenosine Triphosphatases; Adenosine Triphosphate; Animals; Bridged Bicyclo Compounds; Cysteine; Dose-Response Relationship, Drug; Escherichia coli; Escherichia coli Proteins; Fluorescent Dyes; HSP70 Heat-Shock Proteins; Iron-Sulfur Proteins; Kinetics; Microscopy, Fluorescence; Models, Chemical; Models, Molecular; Molecular Chaperones; Mutagenesis, Site-Directed; Mutation; Peptides; Protein Binding; Protein Isoforms; Protein Structure, Tertiary; Rats; Spectrometry, Fluorescence; Tryptophan | 2004 |
Human deafness mutation of myosin VI (C442Y) accelerates the ADP dissociation rate.
Topics: Actins; Adenosine Diphosphate; Adenosine Triphosphatases; Adenosine Triphosphate; Animals; Calcium; Deafness; Humans; Mice; Molecular Motor Proteins; Mutation, Missense; Myosin Heavy Chains; Protein Binding; Tryptophan | 2004 |
Musajo Memorial Award Lecture: "Metabolism to function--an odyssey with tryptophan".
Topics: Adenosine Diphosphate; Animals; History, 20th Century; Humans; Mixed Function Oxygenases; Tryptophan; Tryptophan Oxygenase | 2003 |
An intermediate form of ADP-F-actin.
Topics: Actins; Adenosine Diphosphate; Adenosine Triphosphate; Biopolymers; Fluorescence; Fungal Proteins; Hydrogen-Ion Concentration; Hydrolysis; Muscles; Mutation; Phosphates; Protein Structure, Quaternary; Time Factors; Tryptophan; Yeasts | 2005 |
Does phosphate release limit the ATPases of soleus myofibrils? Evidence that (A)M. ADP.Pi states predominate on the cross-bridge cycle.
Topics: Adenosine Diphosphate; Adenosine Triphosphatases; Adenosine Triphosphate; Animals; Calcium; Models, Biological; Muscle Contraction; Muscle, Skeletal; Myofibrils; Phosphates; Psoas Muscles; Rabbits; Temperature; Tryptophan | 2004 |
The effect of F-actin on the relay helix position of myosin II, as revealed by tryptophan fluorescence, and its implications for mechanochemical coupling.
Topics: Actins; Actomyosin; Adenosine Diphosphate; Adenosine Triphosphate; Animals; Dictyostelium; Molecular Motor Proteins; Myosin Type II; Protein Binding; Protein Structure, Secondary; Protein Structure, Tertiary; Protozoan Proteins; Rabbits; Signal Transduction; Spectrometry, Fluorescence; Tryptophan | 2004 |
The binding mechanism of the yeast F1-ATPase inhibitory peptide: role of catalytic intermediates and enzyme turnover.
Topics: Adenosine Diphosphate; Adenosine Triphosphatases; Adenosine Triphosphate; ATPase Inhibitory Protein; Binding Sites; Biochemistry; Catalysis; Catalytic Domain; Dose-Response Relationship, Drug; Escherichia coli; Hydrolysis; Kinetics; Models, Biological; Models, Chemical; Protein Binding; Proteins; Saccharomyces cerevisiae; Spectrometry, Fluorescence; Time Factors; Tryptophan | 2005 |
Structural basis for the changes in redox potential in the nitrogenase Phe135Trp Fe protein with MgADP Bound.
Topics: Adenosine Diphosphate; Azotobacter vinelandii; Binding Sites; Catalysis; Ions; Iron-Sulfur Proteins; Magnesium; Models, Chemical; Models, Molecular; Models, Statistical; Nitrogenase; Oxidation-Reduction; Phenylalanine; Protein Binding; Protein Conformation; Tryptophan; X-Ray Diffraction | 2004 |
Binding of six nucleotide cofactors to the hexameric helicase RepA protein of plasmid RSF1010. 1. Direct evidence of cooperative interactions between the nucleotide-binding sites of a hexameric helicase.
Topics: Adenosine Diphosphate; Adenosine Triphosphate; Binding Sites; Coenzymes; DNA Helicases; DNA Replication; DNA-Binding Proteins; Models, Chemical; ortho-Aminobenzoates; Plasmids; Protein Subunits; Spectrometry, Fluorescence; Thermodynamics; Trans-Activators; Tryptophan | 2005 |
Examination of MgATP binding in a tryptophan-shift mutant of phosphofructokinase from Bacillus stearothermophilus.
Topics: Adenosine Diphosphate; Adenosine Triphosphate; Allosteric Site; Base Sequence; Binding Sites; Fructosephosphates; Genetic Variation; Geobacillus stearothermophilus; Mutation; Phosphoenolpyruvate; Phosphofructokinases; Spectrometry, Fluorescence; Tryptophan | 2005 |
Correlation between conformational stability of the ternary enzyme-substrate complex and domain closure of 3-phosphoglycerate kinase.
Topics: Adenosine Diphosphate; Adenosine Triphosphate; Animals; Calorimetry; Enzyme Stability; Fluorometry; Hot Temperature; Models, Molecular; Multiprotein Complexes; Muscles; Mutation; Phosphoglycerate Kinase; Protein Binding; Protein Denaturation; Protein Folding; Protein Structure, Tertiary; Structural Homology, Protein; Swine; Thermodynamics; Tryptophan; Yeasts | 2005 |
Structural rearrangements in the active site of smooth-muscle myosin.
Topics: Acrylamide; Actins; Adenosine Diphosphate; Adenosine Triphosphatases; Adenosine Triphosphate; Animals; Binding Sites; Cell Line; Chickens; Crystallography, X-Ray; Dictyostelium; DNA, Complementary; Dose-Response Relationship, Drug; Fluorescence Resonance Energy Transfer; Hydrolysis; Insecta; Kinetics; Models, Chemical; Models, Molecular; Mutagenesis, Site-Directed; Mutation; Myosin Type II; Myosins; Nucleotides; Protein Conformation; Protein Structure, Tertiary; Temperature; Time Factors; Tryptophan | 2005 |
Nucleotides regulate the conformational state of the small terminase subunit from bacteriophage lambda: implications for the assembly of a viral genome-packaging motor.
Topics: Acrylamide; Adenosine Diphosphate; Adenosine Triphosphate; Azides; Bacteriophage lambda; Binding, Competitive; Dimerization; DNA-Binding Proteins; DNA, Viral; Endodeoxyribonucleases; Genome, Viral; Molecular Motor Proteins; Photoaffinity Labels; Protein Binding; Protein Conformation; Protein Subunits; Sequence Deletion; Spectrometry, Fluorescence; Tryptophan; Viral Proteins; Virus Assembly | 2005 |
Functional characterization and ATP-induced dimerization of the isolated ABC-domain of the haemolysin B transporter.
Topics: Adenosine Diphosphate; Adenosine Triphosphatases; Adenosine Triphosphate; Alanine; ATP-Binding Cassette Transporters; Bacterial Proteins; Buffers; Carrier Proteins; Dimerization; Escherichia coli Proteins; Hemolysin Proteins; Histidine; Hydrogen-Ion Concentration; Hydrolysis; Models, Chemical; Mutagenesis, Site-Directed; Peptide Fragments; Protein Binding; Protein Structure, Tertiary; Tryptophan; Tyrosine | 2005 |
The kinetic study of arginine kinase from the sea cucumber Stichopus japonicus with 5,5'-dithiobis-(2-nitrobenzoic acid).
Topics: Adenosine Diphosphate; Adenosine Triphosphate; Animals; Arginine Kinase; Binding Sites; Catalysis; Cysteine; Dimerization; Dithionitrobenzoic Acid; Dithiothreitol; Dose-Response Relationship, Drug; Kinetics; Magnesium; Models, Chemical; Protein Binding; Protein Conformation; Protein Structure, Tertiary; Sea Cucumbers; Spectrometry, Fluorescence; Substrate Specificity; Sulfhydryl Compounds; Time Factors; Tryptophan | 2005 |
Removal of tightly bound ADP induces distinct structural changes of the two tryptophan-containing regions of the ncd motor domain.
Topics: Adenosine Diphosphate; Adenosine Triphosphatases; Animals; Binding Sites; Circular Dichroism; Drosophila Proteins; Kinesins; Models, Molecular; Mutation; Phenylalanine; Protein Structure, Tertiary; Spectrometry, Fluorescence; Spectrophotometry, Ultraviolet; Tryptophan; Tubulin; Tyrosine | 2005 |
Did protein kinase regulatory mechanisms evolve through elaboration of a simple structural component?
Topics: Adenosine Diphosphate; Amino Acid Motifs; Amino Acid Sequence; Animals; Binding Sites; Databases, Protein; Evolution, Molecular; Glutamic Acid; Histidine; Humans; Models, Genetic; Models, Molecular; Molecular Conformation; Molecular Sequence Data; Monte Carlo Method; Protein Conformation; Protein Kinases; Protein Structure, Tertiary; Sequence Homology, Amino Acid; Tryptophan; Tyrosine; Water | 2005 |
Docking and rolling, a model of how the mitotic motor Eg5 works.
Topics: Adenosine Diphosphate; Adenosine Triphosphatases; Adenosine Triphosphate; Binding Sites; Carboxylic Acids; Catalytic Domain; Cysteine; Dose-Response Relationship, Drug; Evolution, Molecular; Fluorescence Resonance Energy Transfer; Humans; Kinesins; Kinetics; Microtubules; Mitosis; Models, Biological; Models, Chemical; Models, Molecular; Mutation; Protein Binding; Protein Structure, Tertiary; Tryptophan | 2005 |
Characterization of archaeal group II chaperonin-ADP-metal fluoride complexes: implications that group II chaperonins operate as a "two-stroke engine".
Topics: Adenosine Diphosphate; Adenosine Triphosphatases; Aluminum Compounds; Archaea; Archaeal Proteins; Beryllium; Chaperonin Containing TCP-1; Chaperonins; Cytosol; Fluorides; Green Fluorescent Proteins; Microscopy, Electron; Models, Biological; Nucleotides; Peptide Hydrolases; Protein Binding; Protein Conformation; Protein Folding; Protein Structure, Tertiary; Scattering, Radiation; Spectrometry, Fluorescence; Temperature; Thermococcus; Tryptophan; X-Rays | 2005 |
The nucleotide-binding site of the Escherichia coli DnaC protein: molecular topography of DnaC protein-nucleotide cofactor complexes.
Topics: Adenosine Diphosphate; Adenosine Triphosphate; Binding Sites; Escherichia coli; Escherichia coli Proteins; Hydrophobic and Hydrophilic Interactions; Magnesium Chloride; ortho-Aminobenzoates; Protein Conformation; Spectrometry, Fluorescence; Tryptophan; Tyrosine | 2005 |
Reversal of ADP-mediated aggregation of adenosine kinase by cyclophilin leads to its reactivation.
Topics: Adenosine Diphosphate; Adenosine Kinase; Adenosine Monophosphate; Animals; Base Sequence; Binding Sites; Cyclophilins; Kinetics; Leishmania donovani; Spectrometry, Fluorescence; Time Factors; Tryptophan | 2006 |
Allosteric regulation of Hsp70 chaperones by a proline switch.
Topics: Adenosine Diphosphate; Adenosine Triphosphate; Allosteric Regulation; Amino Acid Substitution; Animals; Binding Sites; HSP70 Heat-Shock Proteins; Hydrogen Bonding; Models, Molecular; Mutagenesis, Site-Directed; Proline; Protein Conformation; Tryptophan | 2006 |
Ligand-induced effects on pyruvate dehydrogenase kinase isoform 2.
Topics: Adenosine Diphosphate; Adenosine Triphosphate; Dimerization; Down-Regulation; Glutathione Transferase; Humans; Ligands; Molecular Conformation; Mutation; Protein Binding; Protein Kinases; Protein Serine-Threonine Kinases; Pyruvate Dehydrogenase Acetyl-Transferring Kinase; Pyruvic Acid; Tryptophan | 2006 |
Conformational and functional characterization of trapped complexes of the P-glycoprotein multidrug transporter.
Topics: Acrylamide; Adenosine Diphosphate; Adenosine Triphosphate; Animals; ATP Binding Cassette Transporter, Subfamily B, Member 1; Cesium; CHO Cells; Cricetinae; Cricetulus; Fluorescence; Iodine; Kinetics; Multidrug Resistance-Associated Proteins; Protein Conformation; Trinitrobenzenes; Tryptophan | 2006 |
Reversible movement of switch 1 loop of myosin determines actin interaction.
Topics: Actins; Adenosine Diphosphate; Adenosine Triphosphate; Animals; Cryoelectron Microscopy; Crystallography, X-Ray; Dictyostelium; Magnesium; Models, Chemical; Models, Molecular; Myosins; Protein Binding; Protein Conformation; Protein Structure, Tertiary; Tryptophan | 2007 |
A mobile tryptophan is the intrinsic charge transfer donor in a flavoenzyme essential for nikkomycin antibiotic biosynthesis.
Topics: Adenosine Diphosphate; Aminoglycosides; Binding Sites; Crystallization; Flavin-Adenine Dinucleotide; Flavoproteins; Mutation; Picolinic Acids; Tryptophan | 2007 |
Studies of nucleotide binding to the catalytic sites of Escherichia coli betaY331W-F1-ATPase using fluorescence quenching.
Topics: Adenosine Diphosphate; Adenosine Triphosphate; Binding Sites; Catalytic Domain; Escherichia coli; Kinetics; Ligands; Magnesium; Mutation; Nucleotides; Phosphates; Protein Binding; Proton-Translocating ATPases; Spectrometry, Fluorescence; Tryptophan | 2007 |
The association of actin and myosin in the presence of gamma-amido-ATP proceeds mainly via a complex with myosin in the closed conformation.
Topics: Actins; Adenosine Diphosphate; Adenylyl Imidodiphosphate; Animals; Dictyostelium; Fluorescence; Myosins; ortho-Aminobenzoates; Protein Conformation; Spectrometry, Fluorescence; Tryptophan | 2007 |
Functional evaluation of conserved basic residues in human phosphomevalonate kinase.
Topics: Adenosine Diphosphate; Adenosine Triphosphate; Amino Acid Sequence; Amino Acids, Basic; Binding Sites; Catalysis; Conserved Sequence; Escherichia coli; Humans; Hydrogen-Ion Concentration; Kinetics; Molecular Sequence Data; Mutation; Phosphotransferases (Phosphate Group Acceptor); Recombinant Proteins; Sequence Homology, Amino Acid; Spectrometry, Fluorescence; Transformation, Genetic; Tryptophan | 2007 |
Identification of the betaTP site in the x-ray structure of F1-ATPase as the high-affinity catalytic site.
Topics: Adenosine Diphosphate; Adenosine Triphosphate; Catalytic Domain; Crystallography, X-Ray; Lysine; Magnesium; Models, Molecular; Mutation; Protein Binding; Protein Structure, Quaternary; Protein Subunits; Proton-Translocating ATPases; Tryptophan | 2007 |
Critical role of specific ions for ligand-induced changes regulating pyruvate dehydrogenase kinase isoform 2.
Topics: Adenosine Diphosphate; Adenosine Triphosphate; Binding Sites; Fluorescence; Humans; Ions; Ligands; Magnesium Chloride; Models, Molecular; Phosphates; Potassium; Protein Binding; Protein Conformation; Protein Serine-Threonine Kinases; Pyruvate Dehydrogenase Acetyl-Transferring Kinase; Pyruvic Acid; Quaternary Ammonium Compounds; Tryptophan | 2008 |
Photophysical properties of popular fluorescent adenosine nucleotide analogs used in enzyme mechanism probing.
Topics: Adenosine Diphosphate; Adenosine Triphosphatases; Adenosine Triphosphate; Animals; Endopeptidase Clp; Escherichia coli Proteins; Fluorescence Polarization; Fluorescent Dyes; Heat-Shock Proteins; HSP70 Heat-Shock Proteins; HSP90 Heat-Shock Proteins; Kinetics; Nucleic Acid Conformation; ortho-Aminobenzoates; Rabbits; Thermus thermophilus; Tryptophan | 2008 |
A mutation (R826W) in nucleotide-binding domain 1 of ABCC8 reduces ATPase activity and causes transient neonatal diabetes.
Topics: Adenosine Diphosphate; Adenosine Triphosphatases; Adenosine Triphosphate; Amino Acid Sequence; Amino Acid Substitution; Arginine; ATP-Binding Cassette Transporters; Child; Diabetes Mellitus; Humans; Infant, Newborn; Infant, Newborn, Diseases; Ion Channel Gating; Kinetics; Male; Molecular Sequence Data; Mutant Proteins; Mutation; Potassium Channels, Inwardly Rectifying; Protein Structure, Secondary; Protein Structure, Tertiary; Receptors, Drug; Sulfonylurea Receptors; Tryptophan | 2008 |
Structural analysis of the human Rad51 protein-DNA complex filament by tryptophan fluorescence scanning analysis: transmission of allosteric effects between ATP binding and DNA binding.
Topics: Adenosine Diphosphate; Adenosine Triphosphate; Allosteric Regulation; Amino Acid Substitution; Animals; Circular Dichroism; DNA; Fluorescence Resonance Energy Transfer; Humans; Models, Molecular; Protein Binding; Protein Engineering; Protein Structure, Tertiary; Protein Subunits; Rad51 Recombinase; Spectrometry, Fluorescence; Tryptophan | 2008 |
Spectroscopic and crystallographic studies of the mutant R416W give insight into the nucleotide binding traits of subunit B of the A1Ao ATP synthase.
Topics: Adenosine Diphosphate; Adenosine Triphosphate; Amino Acid Substitution; Archaeal Proteins; Crystallography, X-Ray; Models, Molecular; Nucleotides; Protein Subunits; Proton-Translocating ATPases; Spectrometry, Fluorescence; Tryptophan | 2009 |
Functional energetic landscape in the allosteric regulation of muscle pyruvate kinase. 2. Fluorescence study.
Topics: Adenosine Diphosphate; Allosteric Regulation; Animals; Energy Metabolism; Entropy; Enzyme Activation; Ligands; Models, Chemical; Muscle, Skeletal; Phenylalanine; Phosphoenolpyruvate; Protein Binding; Protein Structure, Tertiary; Pyruvate Kinase; Rabbits; Spectrometry, Fluorescence; Tryptophan | 2009 |
Does Interaction between the Motor and Regulatory Domains of the Myosin Head Occur during ATPase Cycle? Evidence from Thermal Unfolding Studies on Myosin Subfragment 1.
Topics: Adenosine Diphosphate; Adenosine Triphosphatases; Animals; Calorimetry, Differential Scanning; Fluorescence; Humans; Models, Molecular; Myosin Subfragments; Protein Binding; Protein Denaturation; Protein Structure, Tertiary; Protein Unfolding; Rabbits; Temperature; Tryptophan | 2015 |
Muscle biopsies from human muscle diseases with myopathic pathology reveal common alterations in mitochondrial function.
Topics: Adenosine Diphosphate; Adenosine Triphosphate; Adolescent; Adult; Aged; Aspartate Aminotransferases; Biopsy; Child; Citrate (si)-Synthase; Female; Humans; Malate Dehydrogenase; Male; Middle Aged; Mitochondria; Mitochondrial Proteins; Models, Molecular; Multienzyme Complexes; Muscles; Muscular Diseases; Superoxide Dismutase; Tryptophan; Young Adult | 2016 |