Compounds > adenosine-5--o-(3-thiotriphosphate)

adenosine-5--o-(3-thiotriphosphate) and 3-phosphoglycerate

adenosine-5--o-(3-thiotriphosphate) has been researched along with 3-phosphoglycerate* in 1 studies

Other Studies

1 other study(ies) available for adenosine-5--o-(3-thiotriphosphate) and 3-phosphoglycerate

Article	Year
A radiometric method for the determination of NADH in subpicomole amounts. Journal of biochemical and biophysical methods, 1988, Volume: 15, Issue:6 A radiometric method has been devised for the determination of small quantities of NADH formed in preceding dehydrogenase reactions. In a coupled enzymatic reaction, phosphoglycerate kinase (PGK) catalyzes the transfer of [32P]orthophosphate from [gamma-32P]ATP to 3-phosphoglycerate; the intermediate, 1,3-[1-32P]diphosphoglycerate, is dephosphorylated by glyceraldehyde-3-phosphate dehydrogenase (GAP-DH). [32P]Orthophosphate is released proportionally to NADH and can be measured after adsorption of [gamma-32P]ATP to activated charcoal. With this method, 0.2 pmol of NADH are detectable in the presence of a 10(4)-fold excess of NAD over NADH. Topics: Adenosine Triphosphate; Diphosphoglyceric Acids; Glyceraldehyde-3-Phosphate Dehydrogenases; Glyceric Acids; Hydrogen-Ion Concentration; Microchemistry; NAD; Phosphates; Phosphoglycerate Kinase; Phosphorus Radioisotopes; Radiometry	1988

Article

Year

A radiometric method for the determination of NADH in subpicomole amounts.

Journal of biochemical and biophysical methods, 1988, Volume: 15, Issue:6

A radiometric method has been devised for the determination of small quantities of NADH formed in preceding dehydrogenase reactions. In a coupled enzymatic reaction, phosphoglycerate kinase (PGK) catalyzes the transfer of [32P]orthophosphate from [gamma-32P]ATP to 3-phosphoglycerate; the intermediate, 1,3-[1-32P]diphosphoglycerate, is dephosphorylated by glyceraldehyde-3-phosphate dehydrogenase (GAP-DH). [32P]Orthophosphate is released proportionally to NADH and can be measured after adsorption of [gamma-32P]ATP to activated charcoal. With this method, 0.2 pmol of NADH are detectable in the presence of a 10(4)-fold excess of NAD over NADH.

Topics: Adenosine Triphosphate; Diphosphoglyceric Acids; Glyceraldehyde-3-Phosphate Dehydrogenases; Glyceric Acids; Hydrogen-Ion Concentration; Microchemistry; NAD; Phosphates; Phosphoglycerate Kinase; Phosphorus Radioisotopes; Radiometry

1988