acrylamide has been researched along with tryptophan in 246 studies
| Timeframe | Studies, this research(%) | All Research% |
|---|---|---|
| pre-1990 | 33 (13.41) | 18.7374 |
| 1990's | 82 (33.33) | 18.2507 |
| 2000's | 100 (40.65) | 29.6817 |
| 2010's | 30 (12.20) | 24.3611 |
| 2020's | 1 (0.41) | 2.80 |
| Authors | Studies |
|---|---|
| Martorana, GE; Meucci, E; Miggiano, GA; Mordente, A; Santini, SA | 1 |
| Horowitz, PM; Lee, JC; Yeh, LC | 1 |
| Gonzalez, L; Maegley, KA; Reich, NO; Smith, DW | 1 |
| Cherek, H; Gryczynski, I; Joshi, N; Lakowicz, JR; Szmacinski, H | 1 |
| Berman, HA; Nowak, MW | 1 |
| Karkaria, CE; Rosen, BP; Steiner, RF | 1 |
| Deléage, G; Di Pietro, A; Divita, G; Gautheron, DC; Roux, B | 1 |
| Pain, RH; Varley, PG | 1 |
| Ilich, P; Prendergast, FG | 1 |
| Narasimhulu, S | 2 |
| Drake, SK; Frerman, FE; Loehr, JP; Watmough, NJ | 1 |
| Barkley, MD; Laine, RA; Zhu, BC | 1 |
| Betley, MJ; Singh, BR | 1 |
| Chang, LS; Yang, CC | 1 |
| Auclair, F; Mantsch, HH; Surewicz, K; Surewicz, WK | 1 |
| Eftink, MR; Wasylewski, Z | 1 |
| Berger, JW; Vanderkooi, JM | 1 |
| Deibel, MR; Epps, DE; Yem, AW | 1 |
| D'Onofrio, M; Giganti, MG; Grimaldi, S; Pascale, E; Pozzi, D; Verna, R | 1 |
| Steinberg, M; Tyson, PA | 2 |
| Hug, DH; O'Donnell, PS | 1 |
| Stryjewski, W; Wasylewski, Z | 1 |
| Chen, LX; Fleming, GR; Longworth, JW | 1 |
| Eftink, MR; Hagaman, KA | 3 |
| Eftink, MR; Ghiron, CA | 2 |
| Baudet, S; Jullien, M; Le Bras, G; Rodier, F | 1 |
| Haase, FC; Kumar, GK; Phillips, NF; Wood, HG | 1 |
| Chai, YG; Lee, J; Robinson, GW; Singh, BR; Song, PS | 1 |
| Carlson, W; Epps, DE; Heinrikson, R; Hui, J; Poorman, R | 1 |
| Borkman, RF; Phillips, SR | 1 |
| Augusteyn, RC; Putilina, T; Seifert, R | 1 |
| Lerman, S; Moran, M | 1 |
| Chang, GG; Chen, YH; Lee, HJ | 1 |
| Cherek, H; Gryczynski, I; Johnson, ML; Joshi, N; Lakowicz, JR | 1 |
| Gryczynski, I; Johnson, ML; Joshi, NB; Lakowicz, JR; Szmacinski, H | 1 |
| Behnke, WD; Hundley, P; McIntyre, JC | 1 |
| Borkman, RF; Phillips, SR; Wilson, LJ | 1 |
| Beltramini, M; Lerch, K | 1 |
| Torgerson, PM | 1 |
| Armenian, AG; Gasparian, VK; Mardanian, SS; Nalbandian, RM | 1 |
| Giménez-Gallego, G; Lozano, RM; Muñoz-Willery, I; Núñez De Castro, I; Pineda-Lucena, A; Zazo, M | 1 |
| Haavik, J; Knappskog, PM | 1 |
| Chakrabarti, A; Majee, S | 1 |
| Merrill, AR; Palmer, LR; Szabo, AG | 1 |
| Bandyopadhyay, S; Banik, U; Bhattacharyya, B; Mandal, NC; Roy, S | 1 |
| Anzenbacher, P; Balny, C; Lange, R; Maurin, L; Müller, S | 1 |
| Hedstrom, J; Norman, JA; Prendergast, FG; Punyiczki, M; Rosenberg, A; Somogyi, B | 1 |
| Gryczynski, I; Johnson, ML; Kuśba, J; Lakowicz, JR; Zelent, B | 2 |
| Bujalowski, W; Klonowska, MM | 1 |
| Chang, YC; Ludescher, RD | 2 |
| Augusteyn, RC; Chandrasekher, G; Ghiggino, KP; Vassett, P | 1 |
| Friedman, JM; Harrington, JP; Hirsch, RE; Vidugiris, GJ | 1 |
| Manabe, K; Nakazawa, M; Song, PS; Wells, TA | 1 |
| English, AM; Ferreira-Rajabi, L; Fox, T; Hill, BC | 1 |
| Bhattacharyya, A; Bhattacharyya, B; Roy, S | 1 |
| Cardemil, E; Encinas, MV; Goldie, H; Rojas, MC | 1 |
| Augusteyn, RC; Ghiggino, KP; Putilina, T | 1 |
| Consler, TG; Kaback, HR; Weitzman, C | 1 |
| Billheimer, JT; Colles, SM; McLean, LR; Moncecchi, D; Myers-Payne, SC; Schroeder, F; Woodford, JK | 1 |
| Małecki, J; Wasylewski, M; Wasylewski, Z | 1 |
| Mishra, VK; Palgunachari, MN | 1 |
| Itoh, Y; Nagase, H; Stack, MS; Young, TN | 1 |
| Helmkamp, GM; Tremblay, JM; Voziyan, PA; Yarbrough, LR | 1 |
| Drwiega, M; Kaszycki, P; Wasylewski, Z | 1 |
| Gabrielsen, OS; Hegvold, AB | 1 |
| Bousquet, JA; Ettner, N | 1 |
| Cui, D; Lin, Q; Wang, Q | 1 |
| Fidler, V; Fleming, GR; Hof, M | 1 |
| Craescu, CT; Gallay, J; Rouviere, N; Vincent, M | 1 |
| Cheung, HC; Dong, WJ; She, M; Umeda, PK | 1 |
| Awasthi, YC; Bandorowicz-Pikuła, J; Pikuła, S; Wrzosek, A | 1 |
| Gao, P; Sun, YQ; Yan, BX | 1 |
| Cester, N; Curatola, A; Fumelli, P; Kotyk, A; Kvasnicka, P; Mazzanti, L; Rabini, RA; Staffolani, R; Zolese, G | 1 |
| Bieth, JG; Cadène, M; Mély, Y; Sylte, I | 1 |
| Don, Y; Li, H; Liu, N; Wu, H; Yang, K; Zhang, Y | 1 |
| Denhardt, DT; Lackland, H; Ludescher, RD; Rajan, SS | 1 |
| Frère, JM; Guillaume, G; Lejeune, A; Pain, RH; Schmid, FX; Vanhove, M; Virden, R | 1 |
| Kosk-Kosicka, D; Lopez, MM | 1 |
| Blandin, P; Desmadril, M; Garcia, P; Mérola, F; Minard, P; Receveur, V | 1 |
| Breukink, E; de Kruijff, B; Demel, RA; Kuipers, OP; Siezen, RJ; van Dalen, A; van Kraaij, C | 1 |
| Brennan, JD; Hogue, CW; Zheng, L | 1 |
| Bazin, M; Brochon, JC; Jurado, J; Kuznetsov, SV; Laval, J; Santus, R; Sidorkina, OM; Tauc, P | 1 |
| Aleksiev, B; Betzel, C; Dolashka, P; Genov, N; Mancheva, I; Rajashankar, KR | 1 |
| Gallay, J; Lewit-Bentley, A; Sopkova, J; Takahashi, M; Vincent, M | 1 |
| Alvarez, C; Campos, AM; Lanio, ME; Lissi, EA; Martinez, D; Morera, V; Pazos, IF | 1 |
| Busti, P; Delorenzi, NJ; Gatti, CA | 1 |
| Komath, SS; Padma, P; Swamy, MJ | 1 |
| Amrani, A; Azarkan, M; Looze, Y; Nijs, M; Smolders, N; Vincentelli, J; Zerhouni, S | 1 |
| Park, HS; Park, JW | 1 |
| Li, J; Meighen, EA; Szittner, R | 1 |
| Ajtai, K; Burghardt, TP; Park, S | 1 |
| Bain, AJ; Bryant, J; Chadborn, N; O'Shea, P | 1 |
| Imoto, T; Nishimoto, E; Yamasaki, N; Yamashita, S | 1 |
| Kay, CM; Kiss, RS; Ryan, RO | 1 |
| Kleinschmidt, JH; Tamm, LK | 1 |
| Das, I; Datta, AK; Ghosh, M; Sinha, KM | 1 |
| Biktashev, AG; Khaitlina, SY; Kuznetsova, IM; Turoverov, KK | 1 |
| Bułaj, G; Dobryszycki, P; Kochman, M; Rymarczuk, M | 1 |
| Dobryszycki, P; Gapiński, J; Kochman, M; Rymarczuk, M | 1 |
| Ling, V; Ruysschaert, JM; Shapiro, AB; Sonveaux, N; Vigano, C | 1 |
| Engelborghs, Y; Gastmans, M; Volckaert, G | 1 |
| Merrill, AR; Tory, MC | 1 |
| Guzman, G; Miller, TE; Moncrieffe, MC; Potter, JD; Prendergast, FG; Venyaminov, SY | 1 |
| Masaki, K; Mizuguchi, M; Nitta, K | 1 |
| Biktashev, AG; Khaitlina, SY; Kuznetsova, IM; Turoverov, KK; Uversky, VN; Vassilenko, KS | 1 |
| Dirr, HW; Wallace, LA | 2 |
| Behere, DV; Deva, MS | 1 |
| Anderluh, G; Barlic, A; MaCek, P; Malovrh, P; Menestrina, G; Podlesek, Z | 1 |
| France, RM; Grossman, SH | 1 |
| Bandyopadhyay, S; Deb, S; Roy, S | 1 |
| Senior, AE; Weber, J | 1 |
| Kay, C; Prenner, EJ; Ryan, RO; Weers, PM | 1 |
| Barnes, JA; Gomes, AV; Vogel, HJ | 1 |
| Burghardt, TP; Park, S | 1 |
| Mitra, S; Sau, AK | 1 |
| Gelamo, EL; Tabak, M | 1 |
| Liu, R; Sharom, FJ; Siemiarczuk, A | 1 |
| Fidy, J; Laberge, M; Polgár, L; Szeltner, Z; Tölgyesi, F; Ullrich, B | 1 |
| Abugo, O; Collins, JH; Digel, J; Gryczynski, Z; Kobayashi, T; Lakowicz, JR | 1 |
| Duncan, MW; Golding, J; Henderson, T; Hochgrebe, T; Hou, JY; Lynch, G; Poljak, A; Zoellner, H | 1 |
| Choi, G; Choi, KY; Ha, NC; Hong, BH; Kim, MS; Oh, BH | 1 |
| D'Auria, S; Di Cesare, N; Gryczynski, I; Lakowicz, JR; Rossi, M | 1 |
| Beal, PA; Stephens, OM; Yi-Brunozzi, HY | 1 |
| Chen, CH; Martin, DL; Rust, E | 1 |
| Corbalán-García, S; del Mar Martínez-Senac, M; Gómez-Fernández, JC | 1 |
| Chang, CC; Chang, LS; Lin, SR; Wang, JJ | 1 |
| Counts Gerber, N; Kosarikov, DN; Lee, JM; Uversky, VN | 1 |
| Pinheiro, TJ; Sanghera, N | 1 |
| Bhattacharyya, D; Brahma, A; Mukherjee, C; Nayar, S | 1 |
| Kasturi, SR; Kuchroo, K; Maity, H | 1 |
| Cioni, P; Strambini, GB | 1 |
| Bandyopadhyay, S; Bose, S; Deb, S; Roy, S | 1 |
| Bera, AK; Bernhardt, R; Fischer, B; Hannemann, F; Lisurek, M; Teuchner, K | 1 |
| Breukink, E; de Kruijff, B; van Heusden, HE | 1 |
| Jarori, GK; Maity, H | 1 |
| Cardemil, E; Espinosa, V; Kettlun, AM; Valenzuela, MA; Zanocco, A | 1 |
| Chatterji, D; Ghosh, P; Ramakrishnan, C | 1 |
| Bettati, S; Campanini, B; Cook, PF; Hazlett, TL; Mozzarelli, A; Raboni, S; Vaccari, S; Zhang, L | 1 |
| Federkeil, SL; Jickling, G; Turner, RJ; Winstone, TL | 1 |
| De Maeyer, M; Engelborghs, Y; Hao, Q; Hellings, M; Peumans, WJ; Van Damme, EJ; Verheyden, S | 1 |
| Broos, J; Gabellieri, E; Jackson, JB; Strambini, GB; van Boxel, GI | 1 |
| Genov, N; Idakieva, K; Parvanova, K; Todinova, S | 1 |
| Arellano, JB; Balsera, M; De Las Rivas, J; Devos, D; Pazos, F; Valencia, A | 1 |
| Hidaka, H; Iwamoto, Y; Muramatsu, T; Oda, T | 1 |
| Fraaije, MW; Janssen, DB; Lutje Spelberg, JH; Tang, L; van Merode, AE | 1 |
| Kadoya, A; Miyake, H; Ohyashiki, T | 1 |
| Iannuzzi, C; Irace, G; Malmo, C; Sirangelo, I | 1 |
| Kusior, D; Olszowska, E; Olszowski, S; Piwowarczyk, M; Stelmaszynska, T | 1 |
| Bravo, A; Gómez, I; Miranda-CassoLuengo, R; Muñoz-Garay, C; Rausell, C; Rudiño-Piñera, E; Soberón, M | 1 |
| Hahm, KS; Kim, JI; Kim, Y; Lim, SS; Shin, SY; Song, YM; Yang, ST | 1 |
| Jung, SJ; Kim, HE; Lee, BJ; Seo, MD; Won, HS | 1 |
| Canters, GW; Cioni, P; de Waal, E; Strambini, GB | 1 |
| Andrade, SM; Carvalho, TI; Costa, SM; Viseu, MI | 1 |
| Bârzu, O; Gallay, J; Gilles, AM; Li de la Sierra, IM; Munier-Lehmann, H; Renouard, M; Sakamoto, H; Vincent, M | 1 |
| Fang, H; Wang, G; Xia, X | 1 |
| Esbjörner, EK; Goksör, M; Lincoln, P; Nordén, B; Persson, D; Thorén, PE | 1 |
| Cheng, Z; Ching Lee, J; Daly, TJ; Herman, P; Surendran, R | 1 |
| Ahmad, B; Ashraf, MT; Khan, RH; Naseem, F | 1 |
| Gensch, T; Hellingwerf, KJ; Hendriks, J | 1 |
| Kinne, RK; Kipp, H; Raja, MM | 1 |
| Fernández, M; Fuentes, L; Oyola, J; Quiñones, E | 1 |
| Davidson, WS; Horace, EM; Jandacek, RJ; Maiorano, JN | 1 |
| Driessen, AJ; Gbaguidi, B; Konings, WN; Mazurkiewicz, P; Ruysschaert, JM; Vigano, C | 1 |
| Kazakov, VI; Kuznetsova, IM; Shavlovsky, MM; Stepanenko, OV; Turoverov, KK; Uversky, VN; Verkhusha, VV | 1 |
| Bren, N; Burghardt, TP; Gao, F; Hansen, S; Henchman, RH; McCammon, JA; Sine, SM; Taylor, P | 1 |
| An, J; Bao, JK; Chen, F; Gao, S; Gu, Y; Wu, CF; Wu, QQ; Yu, Y | 1 |
| Japelj, B; Jerala, R; Majerle, A; Pristovsek, P | 1 |
| Nicholls, IA; Olofsson, L; Wikman, S | 1 |
| Igarashi, K; Kawano, K; Matsubara, K; Matsuura, A; Mizuguchi, M; Mori, Y; Saitoh, T; Shinoda, H; Shinohara, Y; Takeuchi, M | 1 |
| Chen, J; Pang, Y; Wu, P; Zhu, H | 1 |
| den Blaauwen, T; Driessen, AJ; Natale, P; van der Does, C | 1 |
| Berger, CL; Chrin, LR; Gaffney, DP; Robertson, CI | 1 |
| Catalano, CE; Gaussier, H; Maluf, NK; Ortega, ME | 1 |
| Chattopadhyay, A; Kelkar, DA; Rawat, SS | 1 |
| Chen, A; Greenfield, NJ; He, H; Nair, SK; Thomas, T; Thomas, TJ | 1 |
| Hemminga, MA; Hesselink, RW; Koehorst, RB; Nazarov, PV | 1 |
| Crespo, JG; Lima, JC; Portugal, CA | 1 |
| Ahmad, B; Ansari, MA; Khan, RH; Sen, P | 1 |
| Jenei, A; Mátyus, L; Szöllosi, J | 1 |
| Bodner, N; Dusa, A; Edridge, S; Fink, AL; Hong, DP; Kaylor, J | 1 |
| Ceulemans, A; De Maeyer, M; Engelborghs, Y; Hellings, M; Moors, SL | 1 |
| Bankston, JR; Glaaser, IW; Kass, RS; Liu, H; Tateyama, M | 1 |
| Russell, PL; Sharom, FJ | 1 |
| Chaudhuri, TK; Deb, JK; Jana, S | 1 |
| Chattopadhyay, A; Kelkar, DA | 1 |
| Acebal, C; Arroyo, M; Campillo, N; Castillón, MP; de la Mata, I; García, JL; Hormigo, D; Menéndez, M | 1 |
| Arora-Sharawat, A; Chattopadhyay, A | 1 |
| Gaikwad, SM; Shashidhara, KS | 1 |
| Alston, RW; Grimsley, GR; Lasagna, M; Pace, CN; Reinhart, GD; Scholtz, JM | 1 |
| Fedorov, A; Hemminga, MA; Hesselink, RW; Prieto, M | 1 |
| Domanov, Y; Kinnunen, PK; Kontinen, VP; Pietiäinen, M; Sood, R | 1 |
| He, XW; Li, WY; Qin, L; Zhang, YK | 1 |
| Dong, A; Ji, C; Kong, J; Li, H; Ou, Y; Shen, X; Tao, W; Yu, S | 1 |
| Herman, P; Vecer, J | 1 |
| Browne, H; Falanga, A; Fattorusso, R; Galdiero, M; Galdiero, S; Isernia, C; Pedone, C; Raiola, L; Vitiello, M | 1 |
| Cordeiro-da-Silva, A; de Castro, B; Gameiro, P; Lima, SA | 1 |
| Gonnelli, M; Strambini, GB | 4 |
| Anderson, SR; Broderick, D; Deinzer, ML; Harder, ME; Leid, M; Malencik, DA; Schimerlik, MI; Yan, X | 1 |
| Bourbon-Freie, A; Dub, RE; Lowe, ME; Xiao, X | 1 |
| Boone, TC; Carpenter, JF; Kerwin, BA; Mason, BD; Roy, S; Schöneich, CS | 1 |
| Biverståhl, H; Bodor, A; Lind, J; Mäler, L | 1 |
| Narahari, A; Swamy, MJ | 1 |
| Jung, HH; Kim, HJ; Kim, JI; Lee, JY; Lee, SK; Shin, SY; Yang, ST | 1 |
| Gaikwad, S; Gowda, NM; Kumar, A; Pundle, A | 1 |
| Ju, H; Lei, J; Ouyang, R; Qu, P | 1 |
| de Foresta, B; Gallay, J; Garrigos, M; Vincent, M | 1 |
| Blom, SM; Jensen, HS; Schmitt, N | 1 |
| Ansari, MA; Anwar, T; Atif, SM; Iqbal, A; Kashif, M; Khan, N; Owais, M; Rehan, M; Zubair, S | 1 |
| Falanga, A; Galdiero, M; Galdiero, S; Isernia, C; Pedone, C; Raiola, L; Russo, L; Vitiello, M | 1 |
| Chattopadhyay, A; Chaudhuri, A; Haldar, S; Kelkar, DA | 1 |
| Wang, SS; Wen, WS | 1 |
| Daubner, SC; Fitzpatrick, PF; Lasagna, M; Reinhart, GD; Wang, S | 1 |
| Chen, Y; Erickson, HP | 1 |
| Bauer, CE; Dragnea, V; Gardner, KH; Wu, Q; Yuan, H | 1 |
| Chandra, V; Dave, S; Gupta, P; Mahajan, S | 1 |
| Cantisani, M; D'Errico, G; Falanga, A; Galdiero, M; Galdiero, S; Perillo, E; Tarallo, R; Vitiello, G; Vitiello, M | 1 |
| Ghosh, G; Mandal, DK | 1 |
| Ho, J; Wong, KF | 1 |
| Breukink, E; Irving, N; Mantovani, HC; Paiva, AD | 1 |
| Iram, A; Naeem, A | 1 |
| Ahmad, E; Ashraf, R; Bhushan, B; Chaturvedi, SK; Khan, JM; Khan, RH; Qadeer, A; Rabbani, G | 1 |
| Amoussouvi, A; Haralampiev, I; Herrmann, A; Stöckl, M; Wietek, J | 1 |
| He, F; Joshi, SB; Kerwin, BA; Middaugh, CR; Sahni, N; Thakkar, SV; Volkin, DB | 1 |
| Mandal, DK; Sen, D | 1 |
| Podile, AR; Swamy, MJ; Tarafdar, PK; Vedantam, LV | 1 |
| Auton, M; Blancas-Mejia, LM; Madde, P; Tischer, A | 1 |
| Klinman, JP; Meadows, CW; Ou, R | 1 |
| Ahmad, A; Azmi, S; Ghosh, JK; Kumar, A; Mishra, NN; Shukla, PK; Srivastava, S; Tripathi, JK | 1 |
| Wang, Y; Yu, LP; Zhang, R | 1 |
| Dong, W; Ma, L; Shang, D; Sun, L; Sun, Y | 1 |
| Chattopadhyay, K; Ghosh, R; Ghosh, S; Mukherjee, M | 1 |
| Hilt, S; Longo, ML; Risbud, SH; Voss, JC; Zeno, WF | 1 |
| Chaari, A; Chevillot-Biraud, A; Fahy, C; Rholam, M | 1 |
| Breydo, L; Miti, T; Muschol, M; Niyangoda, C; Uversky, V | 1 |
| Ghosh, R; Ghosh, S; Mukherjee, M; Mukherjee, P; Mukhopadhyay, TK; Roy, P; Sardar, PS | 1 |
2 review(s) available for acrylamide and tryptophan
| Article | Year |
|---|---|
Fluorescence quenching studies with proteins.
Topics: Acetamides; Acrylamide; Acrylamides; Alcohol Oxidoreductases; Azurin; Chemical Phenomena; Chemistry, Physical; Ethylene Chlorohydrin; Fluorescence; Hydrogen Peroxide; Indoles; Kinetics; Liver; Luminescent Measurements; Mathematics; Plant Proteins; Proteins; Pyridinium Compounds; Sodium Iodide; Succinimides; Tryptophan; Tyrosine | 1981 |
Frequency domain fluorometry with pulsed light-emitting diodes.
Topics: Acrylamide; Computers; Equipment Design; Fluorescence Polarization; Fluorometry; Lasers; Light; NAD; Spectrometry, Fluorescence; Spectrophotometry, Ultraviolet; Time Factors; Tryptophan | 2008 |
244 other study(ies) available for acrylamide and tryptophan
| Article | Year |
|---|---|
Conformational stability of bovine alpha-crystallin. Evidence for a destabilizing effect of ascorbate.
Topics: Acrylamide; Acrylamides; Animals; Ascorbic Acid; Cattle; Crystallins; Guanidine; Guanidines; Hot Temperature; Hydrogen-Ion Concentration; In Vitro Techniques; Iodides; Light; Nephelometry and Turbidimetry; Protein Conformation; Protein Denaturation; Scattering, Radiation; Spectrometry, Fluorescence; Tryptophan; Urea | 1992 |
Yeast 5S rRNA binding to ribosomal protein L1a alters the fluorescence of tryptophan residues lying outside the binding site.
Topics: Acrylamide; Acrylamides; Binding Sites; Fluorescence; Fungal Proteins; Potassium Iodide; Protein Binding; Ribosomal Proteins; RNA-Binding Proteins; RNA, Ribosomal, 5S; Saccharomyces cerevisiae; Tryptophan | 1992 |
Cofactor and DNA interactions in EcoRI DNA methyltransferase. Fluorescence spectroscopy and phenylalanine replacement for tryptophan 183.
Topics: Acrylamide; Acrylamides; Base Sequence; DNA; Fluorescence Polarization; Genetic Vectors; Molecular Sequence Data; Mutagenesis, Site-Directed; Phenylalanine; S-Adenosylmethionine; Site-Specific DNA-Methyltransferase (Adenine-Specific); Tryptophan | 1992 |
Anisotropy decays of single tryptophan proteins measured by GHz frequency-domain fluorometry with collisional quenching.
Topics: Acrylamide; Acrylamides; Adrenocorticotropic Hormone; Amino Acid Sequence; Birefringence; Fluorescence Polarization; Macromolecular Substances; Micrococcal Nuclease; Molecular Sequence Data; Molecular Structure; Oligopeptides; Oxygen; Plant Proteins; Protein Conformation; Ribonuclease T1; Spectrometry, Fluorescence; Tryptophan | 1991 |
Fluorescence studies on the interactions of myelin basic protein in electrolyte solutions.
Topics: Acrylamide; Acrylamides; Animals; Brain Chemistry; Cattle; Diffusion; Electrolytes; Fluorescence; Macromolecular Substances; Myelin Basic Protein; Protein Conformation; Spectrum Analysis; Tryptophan; Urea | 1991 |
Ligand interactions in the ArsA protein, the catalytic component of an anion-translocating adenosinetriphosphatase.
Topics: Acrylamide; Acrylamides; Adenosine Triphosphatases; Adenosine Triphosphate; Anions; Arsenite Transporting ATPases; Catalysis; Escherichia coli; Fluorescence Polarization; Ion Pumps; Ligands; Magnesium; Multienzyme Complexes; Protein Conformation; Tryptophan | 1991 |
Intrinsic tryptophan fluorescence of Schizosaccharomyces pombe mitochondrial F1-ATPase. A powerful probe for phosphate and nucleotide interactions.
Topics: Acrylamide; Acrylamides; Adenosine Diphosphate; Adenosine Triphosphate; Guanidine; Guanidines; Iodides; Macromolecular Substances; Mitochondria; Protein Conformation; Proton-Translocating ATPases; Schizosaccharomyces; Spectrometry, Fluorescence; Tryptophan | 1991 |
Relation between stability, dynamics and enzyme activity in 3-phosphoglycerate kinases from yeast and Thermus thermophilus.
Topics: Acrylamide; Acrylamides; Enzyme Stability; Hot Temperature; Kinetics; Phosphoglycerate Kinase; Protein Conformation; Saccharomyces cerevisiae; Spectrometry, Fluorescence; Thermodynamics; Thermus; Tryptophan | 1991 |
Electronic states of the indole-acrylamide molecular pair.
Topics: Acrylamide; Acrylamides; Electrochemistry; Indoles; Photochemistry; Spectrometry, Fluorescence; Tryptophan | 1991 |
Inhibition of substrate binding to the adrenal cytochrome P450C-21 by acrylamide and its implications for solvent accessibility of the binding site in the microsomes.
Topics: 17-alpha-Hydroxyprogesterone; Acrylamide; Acrylamides; Adrenal Cortex; Animals; Binding Sites; Cattle; Hydroxyprogesterones; Intracellular Membranes; Kinetics; Membrane Lipids; Microsomes; Solvents; Spectrometry, Fluorescence; Steroid 21-Hydroxylase; Tryptophan | 1991 |
Tryptophan fluorescence in electron-transfer flavoprotein:ubiquinone oxidoreductase: fluorescence quenching by a brominated pseudosubstrate.
Topics: Acrylamide; Acrylamides; Animals; Cesium; Chlorides; Electron-Transferring Flavoproteins; Fatty Acid Desaturases; Flavoproteins; Hydrogen-Ion Concentration; In Vitro Techniques; Iron-Sulfur Proteins; Kinetics; Multienzyme Complexes; Oxidation-Reduction; Oxidoreductases Acting on CH-NH Group Donors; Sodium Iodide; Spectrometry, Fluorescence; Structure-Activity Relationship; Submitochondrial Particles; Swine; Tryptophan; Ubiquinone; Urea | 1991 |
Intrinsic tryptophan fluorescence measurements suggest that polylactosaminyl glycosylation affects the protein conformation of the gelatin-binding domain from human placental fibronectin.
Topics: Acrylamide; Acrylamides; Amino Sugars; beta-Galactosidase; Binding Sites; Chymotrypsin; Electrophoresis, Polyacrylamide Gel; Female; Fibronectins; Gelatin; Glycoproteins; Glycoside Hydrolases; Glycosylation; Humans; Mathematics; Placenta; Protein Conformation; Spectrometry, Fluorescence; Tryptophan | 1990 |
Comparative structural analysis of staphylococcal enterotoxins A and E.
Topics: Acrylamide; Acrylamides; Amino Acid Sequence; Chemical Phenomena; Chemistry, Physical; Circular Dichroism; Enterotoxins; Epitopes; Molecular Sequence Data; Protein Conformation; Spectrometry, Fluorescence; Tryptophan | 1989 |
Assessment of the role of tryptophan residues in phospholipase A2 by fluorescence quenching.
Topics: Acrylamide; Acrylamides; Binding Sites; Elapid Venoms; Fluorescence; Iodides; Phospholipases A; Phospholipases A2; Substrate Specificity; Tryptophan | 1989 |
Interaction of Shigella toxin with globotriaosyl ceramide receptor-containing membranes: a fluorescence study.
Topics: Acrylamide; Acrylamides; Bacterial Toxins; Dimyristoylphosphatidylcholine; Globosides; Glycosphingolipids; Lipid Bilayers; Liposomes; Phosphatidylcholines; Protein Conformation; Shiga Toxins; Shigella dysenteriae; Spectrometry, Fluorescence; Trihexosylceramides; Tryptophan | 1989 |
Fluorescence lifetime and solute quenching studies with the single tryptophan containing protein parvalbumin from codfish.
Topics: Acrylamide; Acrylamides; Animals; Calcium; Fishes; Kinetics; Muscle Proteins; Parvalbumins; Spectrometry, Fluorescence; Tryptophan | 1989 |
Characterization of lens alpha-crystallin tryptophan microenvironments by room temperature phosphorescence spectroscopy.
Topics: Acrylamide; Acrylamides; Crystallins; Guanidine; Guanidines; Iodides; Luminescent Measurements; Nitrites; Protein Conformation; Protein Denaturation; Spectrum Analysis; Temperature; Thermodynamics; Tryptophan; Urea | 1989 |
Characterization of the tryptophan environments of interleukins 1 alpha and 1 beta by fluorescence quenching and lifetime measurements.
Topics: Acrylamide; Acrylamides; Cesium; Electrophoresis, Polyacrylamide Gel; Humans; Interleukin-1; Iodides; Kinetics; Protein Conformation; Recombinant Proteins; Spectrometry, Fluorescence; Tryptophan | 1989 |
Effect of ouabain binding on the fluorescent properties of the Na+/K+-ATPase.
Topics: Acrylamide; Acrylamides; Animals; Fluorescence; Guanidine; Guanidines; Hydrogen-Ion Concentration; Iodides; Ouabain; Protein Conformation; Sodium-Potassium-Exchanging ATPase; Swine; Tryptophan | 1988 |
Quenching of the tryptophan fluorescence of Na,K-ATPase with acrylamide.
Topics: Acrylamide; Acrylamides; Animals; Chymotrypsin; Dogs; Kidney; Kinetics; Protein Conformation; Sodium-Potassium-Exchanging ATPase; Spectrometry, Fluorescence; Tryptophan | 1988 |
Tryptophanyl fluorescence quenching of urocanase from Pseudomonas putida by acrylamide, cesium, iodide, and imidazolepropionate.
Topics: Acrylamide; Acrylamides; Cesium; Hydro-Lyases; Imidazoles; Iodides; Kinetics; Pseudomonas; Spectrometry, Fluorescence; Tryptophan; Urocanate Hydratase | 1985 |
The resolution of heterogeneous fluorescence of multitryptophan-containing proteins studied by a fluorescence-quenching method.
Topics: Acrylamide; Acrylamides; Alcohol Dehydrogenase; Alcohol Oxidoreductases; Animals; Fluorescence; Horses; Muramidase; Phosphoglycerate Kinase; Protein Conformation; Proteins; Tryptophan; X-Ray Diffraction | 1986 |
Accessibility of tryptophan residues in Na,K-ATPase.
Topics: Acrylamide; Acrylamides; Adenosine Diphosphate; Animals; Chymotrypsin; Dogs; In Vitro Techniques; Ouabain; Potassium; Protein Conformation; Protein Denaturation; Sodium; Sodium-Potassium-Exchanging ATPase; Spectrometry, Fluorescence; Tryptophan | 1987 |
Picosecond time-resolved fluorescence of ribonuclease T1. A pH and substrate analogue binding study.
Topics: Acrylamide; Acrylamides; Binding Sites; Endoribonucleases; Fluorescence Polarization; Guanine Nucleotides; Guanosine Monophosphate; Kinetics; Protein Binding; Protein Conformation; Ribonuclease T1; Spectrometry, Fluorescence; Time Factors; Tryptophan; X-Ray Diffraction | 1987 |
Viscosity dependence of the solute quenching of the tryptophanyl fluorescence of proteins.
Topics: Acrylamide; Acrylamides; Glucagon; Parvalbumins; Protein Conformation; Proteins; Ribonuclease T1; Solvents; Spectrometry, Fluorescence; Tryptophan; Viscosity | 1986 |
Frequency domain measurements of the fluorescence lifetime of ribonuclease T1.
Topics: Acrylamide; Acrylamides; Aspergillus oryzae; Calorimetry; Endoribonucleases; Kinetics; Ribonuclease T1; Spectrometry, Fluorescence; Time Factors; Tryptophan | 1987 |
Allosteric transition of aspartokinase I-homoserine dehydrogenase I studied by time-resolved fluorescence.
Topics: Acrylamide; Acrylamides; Allosteric Regulation; Aspartokinase Homoserine Dehydrogenase; Energy Transfer; Escherichia coli; Fluoroimmunoassay; Multienzyme Complexes; Protein Conformation; Quantum Theory; Spectrometry, Fluorescence; Threonine; Tryptophan | 1988 |
Involvement and identification of a tryptophanyl residue at the pyruvate binding site of transcarboxylase.
Topics: Acrylamide; Acrylamides; Amino Acid Sequence; Binding Sites; Carboxyl and Carbamoyl Transferases; Dinitrobenzenes; Molecular Sequence Data; Propionibacterium; Protein Conformation; Pyruvates; Pyruvic Acid; Spectrometry, Fluorescence; Sulfenic Acids; Transferases; Tryptophan | 1988 |
A photoreversible conformational change in 124 kDa Avena phytochrome.
Topics: Acrylamide; Acrylamides; Anions; Cations, Monovalent; Cesium; Chlorides; Edible Grain; Fluorescence; Fluorescent Dyes; Light; Phytochrome; Plant Proteins; Potassium Iodide; Protein Conformation; Seeds; Spectrometry, Fluorescence; Tryptophan | 1988 |
Quenching of tryptophanyl fluorescence of bovine adrenal P-450C-21 and inhibition of substrate binding by acrylamide.
Topics: Acrylamide; Acrylamides; Adrenal Cortex; Animals; Cattle; Kinetics; Microsomes; Protein Binding; Protein Conformation; Spectrometry, Fluorescence; Steroid 21-Hydroxylase; Steroid Hydroxylases; Tryptophan | 1988 |
Inaccessibility of tryptophan residues of recombinant human renin to quenching agents.
Topics: Acrylamide; Acrylamides; Cesium; Chlorides; Fluorescence; Glycosylation; Humans; Iodides; Protein Conformation; Protein Denaturation; Recombinant Proteins; Renin; Spectrometry, Fluorescence; Tryptophan | 1987 |
Fluorescence quenching studies of the structures of calf gamma-II, III, and IV crystallins.
Topics: Acrylamide; Acrylamides; Animals; Cattle; Chemical Phenomena; Chemistry; Circular Dichroism; Crystallins; Iodides; Spectrometry, Fluorescence; Tryptophan | 1988 |
Quenching of tryptophan fluorescence in bovine lens proteins by acrylamide and iodide.
Topics: Acrylamide; Acrylamides; Animals; Cattle; Crystallins; Iodides; Oxidation-Reduction; Protein Conformation; Spectrometry, Fluorescence; Tryptophan | 1988 |
Acrylamide and iodide fluorescence quenching studies on whole human lenses and their protein extracts.
Topics: Acrylamide; Acrylamides; Adolescent; Adult; Aged; Aging; Child; Child, Preschool; Crystallins; Fluorescence; Humans; Iodides; Lens, Crystalline; Magnetic Resonance Spectroscopy; Middle Aged; Tryptophan; Ultraviolet Rays | 1988 |
Fluorescence studies on the dissociation and denaturation of pigeon liver malic enzyme.
Topics: Acrylamide; Acrylamides; Algorithms; Animals; Columbidae; Fluorescence; Guanidine; Guanidines; Liver; Malate Dehydrogenase; Molecular Weight; Protein Denaturation; Tryptophan | 1988 |
Enhanced resolution of fluorescence anisotropy decays by simultaneous analysis of progressively quenched samples. Applications to anisotropic rotations and to protein dynamics.
Topics: Acrylamide; Acrylamides; Bee Venoms; Fluorescence Polarization; Indoles; Melitten; Propylene Glycol; Propylene Glycols; Protein Conformation; Tryptophan | 1987 |
Diffusion coefficients of quenchers in proteins from transient effects in the intensity decays.
Topics: Acrylamide; Acrylamides; Diffusion; Fluorescence; Fluorometry; Micrococcal Nuclease; Oxygen; Tryptophan | 1987 |
The role of aromatic side chain residues in micelle binding by pancreatic colipase. Fluorescence studies of the porcine and equine proteins.
Topics: Acrylamide; Acrylamides; Animals; Binding Sites; Cesium; Chlorides; Colipases; Deoxycholic Acid; Horses; Kinetics; Micelles; Pancreas; Potassium Iodide; Protein Binding; Proteins; Spectrometry, Fluorescence; Structure-Activity Relationship; Swine; Taurodeoxycholic Acid; Tryptophan | 1987 |
Acrylamide and iodide fluorescence quenching as a structural probe of tryptophan microenvironment in bovine lens crystallins.
Topics: Acrylamide; Acrylamides; Animals; Cattle; Chemical Phenomena; Chemistry; Crystallins; Fluorescence; Iodides; Models, Biological; Tryptophan | 1986 |
Fluorescence lifetime and anisotropy studies with liver alcohol dehydrogenase and its complexes.
Topics: Acrylamide; Acrylamides; Alcohol Dehydrogenase; Animals; Fluorescence Polarization; Horses; Indoles; Kinetics; Liver; NAD; Protein Binding; Spectrometry, Fluorescence; Tryptophan | 1986 |
Fluorescence quenching of the buried tryptophan residue of cod parvalbumin.
Topics: Acrylamide; Acrylamides; Animals; Fishes; Muscle Proteins; Parvalbumins; Protein Conformation; Spectrometry, Fluorescence; Tryptophan | 1985 |
Fluorescence properties of Neurospora tyrosinase.
Topics: Acrylamide; Acrylamides; Anilino Naphthalenesulfonates; Binding Sites; Catechol Oxidase; Energy Transfer; Fluorescent Dyes; Monophenol Monooxygenase; Neurospora; Neurospora crassa; Potassium Iodide; Spectrometry, Fluorescence; Tryptophan | 1982 |
Tryptophan emission from myosin subfragment 1: acrylamide and nucleotide effect monitored by decay-associated spectra.
Topics: Acrylamide; Acrylamides; Adenosine Triphosphate; Animals; Kinetics; Muscles; Myosin Subfragments; Myosins; Peptide Fragments; Protein Binding; Rabbits; Spectrometry, Fluorescence; Tryptophan | 1984 |
[Luminescent properties of NADPH: adrenodoxin reductase].
Topics: Acrylamide; Acrylamides; Adrenal Cortex; Animals; Apoenzymes; Ferredoxin-NADP Reductase; Flavin-Adenine Dinucleotide; Fluorescence; Luminescent Measurements; Mitochondria; NADH, NADPH Oxidoreductases; Protein Denaturation; Temperature; Tryptophan; Urea | 1984 |
Effect of low pH and heparin on the structure of acidic fibroblast growth factor.
Topics: 3T3 Cells; Acrylamide; Acrylamides; Animals; Cell Division; Fibroblast Growth Factor 1; Heparin; Hydrogen-Ion Concentration; Kinetics; Mice; Models, Structural; Osmolar Concentration; Protein Conformation; Recombinant Proteins; Spectrometry, Fluorescence; Tryptophan | 1994 |
Tryptophan fluorescence of human phenylalanine hydroxylase produced in Escherichia coli.
Topics: Acrylamide; Acrylamides; Base Sequence; Biopterins; DNA, Complementary; Escherichia coli; Humans; In Vitro Techniques; Kinetics; Molecular Sequence Data; Mutagenesis, Site-Directed; Phenylalanine; Phenylalanine Hydroxylase; Polymerase Chain Reaction; Recombinant Fusion Proteins; Spectrometry, Fluorescence; Tryptophan | 1995 |
A DNA-binding antitumor antibiotic binds to spectrin.
Topics: Acrylamide; Acrylamides; Animals; DNA; Fluorescence; Goats; Macromolecular Substances; Magnesium; Plicamycin; Protein Conformation; Spectrin; Spectrometry, Fluorescence; Tryptophan | 1995 |
Acrylamide quenching of the intrinsic fluorescence of tryptophan residues genetically engineered into the soluble colicin E1 channel peptide. Structural characterization of the insertion-competent state.
Topics: Acrylamide; Acrylamides; Amino Acid Sequence; Colicins; Fluorescence; Genetic Engineering; Ion Channels; Molecular Sequence Data; Mutagenesis, Site-Directed; Peptide Fragments; Protein Conformation; Tryptophan | 1993 |
Role of the C-terminal tail region in the self-assembly of lambda-repressor.
Topics: Acrylamide; Acrylamides; Amino Acids; DNA-Binding Proteins; Hydrogen-Ion Concentration; Peptide Mapping; Repressor Proteins; Spectrometry, Fluorescence; Structure-Activity Relationship; Tryptophan; Viral Proteins; Viral Regulatory and Accessory Proteins | 1995 |
Interaction of tryptophan residues of cytochrome P450scc with a highly specific fluorescence quencher, a substrate analogue, compared to acrylamide and iodide.
Topics: Acrylamide; Acrylamides; Amino Acid Sequence; Binding Sites; Cholesterol; Cholesterol Side-Chain Cleavage Enzyme; Fluorescence; Fluorescent Dyes; Molecular Sequence Data; Potassium Iodide; Spectrometry, Fluorescence; Tryptophan | 1994 |
Coupling between external viscosity and the intramolecular dynamics of ribonuclease T1: a two-phase model for the quenching of protein fluorescence.
Topics: Acrylamide; Acrylamides; Fluorescence; Glycerol; Models, Chemical; Ribonuclease T1; Spectrometry, Fluorescence; Tryptophan; Viscosity | 1994 |
Distance-dependent fluorescence quenching of tryptophan by acrylamide.
Topics: Acrylamide; Acrylamides; Kinetics; Mathematics; Models, Theoretical; Spectrometry, Fluorescence; Time Factors; Tryptophan | 1994 |
Close proximity of tryptophan residues and ATP-binding site in Escherichia coli primary replicative helicase DnaB protein. Molecular topography of the enzyme.
Topics: Acrylamide; Acrylamides; Adenine Nucleotides; Bacterial Proteins; Binding Sites; DNA Helicases; DNA Replication; DnaB Helicases; Energy Transfer; Escherichia coli; In Vitro Techniques; Protein Denaturation; Spectrometry, Fluorescence; Spectrophotometry, Ultraviolet; Tryptophan | 1994 |
Local conformation of rabbit skeletal myosin rod filaments probed by intrinsic tryptophan fluorescence.
Topics: Acrylamide; Acrylamides; Animals; Fluorescence Polarization; Muscles; Myosins; Protein Conformation; Rabbits; Time Factors; Tryptophan | 1994 |
Probing the microenvironments of tryptophan residues in the monomeric crystallins of the bovine lens.
Topics: Acrylamide; Acrylamides; Crystallins; Potassium Iodide; Spectrometry, Fluorescence; Tryptophan | 1994 |
Alteration of tryptophan fluorescence properties upon dissociation of Lumbricus terrestris hemoglobin.
Topics: Acrylamide; Acrylamides; Animals; Carboxyhemoglobin; Hemoglobins; Hydrogen-Ion Concentration; Oligochaeta; Oxyhemoglobins; Spectrometry, Fluorescence; Tryptophan | 1994 |
Tryptophan fluorescence quenching in rabbit skeletal myosin rod.
Topics: Acrylamide; Acrylamides; Animals; Iodides; Muscles; Myosins; Protein Conformation; Protein Structure, Secondary; Rabbits; Solvents; Spectrometry, Fluorescence; Tryptophan | 1993 |
A conformational change associated with the phototransformation of Pisum phytochrome A as probed by fluorescence quenching.
Topics: 2-Hydroxy-5-nitrobenzyl Bromide; Acrylamide; Acrylamides; Cesium; Fabaceae; Light; Phytochrome; Plants, Medicinal; Spectrometry, Fluorescence; Thallium; Time Factors; Tryptophan | 1994 |
Quenching of intrinsic fluorescence of yeast cytochrome c peroxidase by covalently- and noncovalently-bound quenchers.
Topics: Acrylamide; Acrylamides; Cesium; Cyanides; Cytochrome-c Peroxidase; Fluorescence; Iodides; Ruthenium; Ruthenium Compounds; Saccharomyces cerevisiae; Tryptophan | 1993 |
A study of colchicine tubulin complex by donor quenching of fluorescence energy transfer.
Topics: Acrylamide; Acrylamides; Alcohol Dehydrogenase; Animals; Colchicine; Dimethyl Sulfoxide; Energy Transfer; Horses; Liver; NAD; Spectrometry, Fluorescence; Tryptophan; Tubulin | 1993 |
Comparative steady-state fluorescence studies of cytosolic rat liver (GTP), Saccharomyces cerevisiae (ATP) and Escherichia coli (ATP) phospho enol pyruvate carboxykinases.
Topics: Acrylamide; Acrylamides; Adenosine Diphosphate; Adenosine Triphosphate; Animals; Binding Sites; Cytosol; Escherichia coli; Fluorescence; Guanosine Triphosphate; Liver; Manganese; Nucleotides; Phosphoenolpyruvate Carboxykinase (GTP); Protein Conformation; Rats; Saccharomyces cerevisiae; Sodium Iodide; Tryptophan | 1993 |
Studies on the location of aromatic amino acids in alpha-crystallin.
Topics: Acrylamide; Acrylamides; Amino Acids; Animals; Cattle; Crystallins; Hydrogen-Ion Concentration; Iodides; Protein Conformation; Spectrometry, Fluorescence; Temperature; Tryptophan; Urea | 1993 |
Fluorescence of native single-Trp mutants in the lactose permease from Escherichia coli: structural properties and evidence for a substrate-induced conformational change.
Topics: Acrylamide; Acrylamides; Amino Acid Sequence; Anilino Naphthalenesulfonates; Escherichia coli; Escherichia coli Proteins; Iodides; Lactose; Membrane Transport Proteins; Molecular Sequence Data; Monosaccharide Transport Proteins; Mutagenesis, Site-Directed; Protein Conformation; Protein Structure, Secondary; Spectrometry, Fluorescence; Structure-Activity Relationship; Sulfhydryl Reagents; Symporters; Tryptophan | 1995 |
Cholesterol interaction with recombinant human sterol carrier protein-2.
Topics: Acrylamide; Acrylamides; Binding Sites; Carrier Proteins; Cholesterol; Circular Dichroism; Drug Interactions; Ergosterol; Fluorometry; Humans; Microsomes; Plant Proteins; Recombinant Proteins; Sterols; Tryptophan | 1995 |
Fluorescence study of Escherichia coli cyclic AMP receptor protein.
Topics: Acrylamide; Acrylamides; Bacterial Proteins; Circular Dichroism; Cyclic AMP; Cyclic AMP Receptor Protein; Escherichia coli; Fluorescence; Iodides; Kinetics; Protein Conformation; Spectrometry, Fluorescence; Tryptophan | 1995 |
Interaction of model class A1, class A2, and class Y amphipathic helical peptides with membranes.
Topics: Acrylamide; Acrylamides; Amino Acid Sequence; Apolipoproteins; Calorimetry, Differential Scanning; Chromatography, High Pressure Liquid; Circular Dichroism; Computer Simulation; Gels; Lipid Bilayers; Membrane Lipids; Molecular Sequence Data; Peptides; Phospholipids; Protein Structure, Secondary; Scattering, Radiation; Spectrometry, Fluorescence; Tryptophan | 1996 |
Fluorescence quenching studies of matrix metalloproteinases (MMPs): evidence for structural rearrangement of the proMMP-2/TIMP-2 complex upon mercurial activation.
Topics: Acrylamide; Acrylamides; Enzyme Activation; Enzyme Precursors; Female; Fluorescence; Gelatinases; Humans; In Vitro Techniques; Macromolecular Substances; Metalloendopeptidases; Molecular Structure; Phenylmercuric Acetate; Protease Inhibitors; Proteins; Spectrometry, Fluorescence; Tissue Inhibitor of Metalloproteinase-2; Tryptophan | 1996 |
Truncations of the C-terminus have different effects on the conformation and activity of phosphatidylinositol transfer protein.
Topics: Acrylamide; Acrylamides; Anilino Naphthalenesulfonates; Animals; Carrier Proteins; Circular Dichroism; Cysteine; Dithionitrobenzoic Acid; Escherichia coli; Kinetics; Membrane Proteins; Mutation; Phosphatidylcholines; Phosphatidylinositols; Phospholipid Transfer Proteins; Protein Conformation; Protein Denaturation; Protein Folding; Protein Structure, Secondary; Rats; Recombinant Proteins; Sequence Deletion; Tryptophan | 1996 |
A fluorescence study of Tn10-encoded tet repressor.
Topics: Acrylamide; Acrylamides; Bacterial Proteins; DNA Nucleotidyltransferases; Escherichia coli; Fluorescence; Helix-Turn-Helix Motifs; Molecular Conformation; Mutation; Potassium Iodide; Repressor Proteins; Spectrometry, Fluorescence; Succinimides; Temperature; Transposases; Tryptophan | 1996 |
The importance of the linker connecting the repeats of the c-Myb oncoprotein may be due to a positioning function.
Topics: Acrylamide; Acrylamides; Animals; Binding Sites; Chickens; Chymotrypsin; Conserved Sequence; DNA Probes; DNA-Binding Proteins; Electrophoresis, Polyacrylamide Gel; Helix-Turn-Helix Motifs; Models, Molecular; Mutagenesis, Site-Directed; Polydeoxyribonucleotides; Protein Structure, Secondary; Proto-Oncogene Proteins; Proto-Oncogene Proteins c-myb; Repetitive Sequences, Nucleic Acid; Spectrometry, Fluorescence; Trans-Activators; Transcription Factors; Trypsin; Tryptophan | 1996 |
A possible tertiary structure change induced by acrylamide in the DNA-binding domain of the Tn10-encoded Tet repressor. A fluorescence study.
Topics: Acrylamide; Acrylamides; DNA-Binding Proteins; Fluorescence Polarization; Helix-Turn-Helix Motifs; Kinetics; Protein Structure, Tertiary; Repressor Proteins; Spectrometry, Fluorescence; Thermodynamics; Tryptophan | 1996 |
Fluorescence studies on the interaction of a synthetic signal peptide and its analog with liposomes.
Topics: Acrylamide; Acrylamides; Amino Acid Sequence; Chymotrypsin; Dithionite; Escherichia coli; Liposomes; Membrane Potentials; Molecular Sequence Data; Phosphatidylcholines; Phosphatidylserines; Phosphoenolpyruvate Sugar Phosphotransferase System; Protein Sorting Signals; Sodium Iodide; Spectrometry, Fluorescence; Tryptophan | 1997 |
Time-resolved fluorescence study of a calcium-induced conformational change in prothrombin fragment 1.
Topics: Acrylamide; Acrylamides; Animals; Calcium; Cattle; Peptide Fragments; Protein Precursors; Prothrombin; Spectrometry, Fluorescence; Tryptophan | 1996 |
Immunosuppressor binding to the immunophilin FKBP59 affects the local structural dynamics of a surface beta-strand: time-resolved fluorescence study.
Topics: Acrylamide; Acrylamides; Carrier Proteins; DNA-Binding Proteins; Fluorescence Polarization; Heat-Shock Proteins; Immunosuppressive Agents; Models, Molecular; Polyenes; Protein Structure, Secondary; Sirolimus; Spectrometry, Fluorescence; Tacrolimus; Tacrolimus Binding Proteins; Temperature; Thermodynamics; Tryptophan | 1997 |
Time-resolved fluorescence study of the single tryptophans of engineered skeletal muscle troponin C.
Topics: Acrylamide; Acrylamides; Animals; Calcium; Chickens; Cloning, Molecular; Crystallography, X-Ray; Fluorescence Polarization; Kinetics; Magnesium; Models, Molecular; Muscle Fibers, Fast-Twitch; Muscle, Skeletal; Mutagenesis, Site-Directed; Protein Conformation; Quantum Theory; Recombinant Proteins; Spectrometry, Fluorescence; Time Factors; Troponin C; Tryptophan | 1997 |
Fluorescence spectroscopic studies on interactions between liver annexin VI and nucleotides--a possible role for a tryptophan residue.
Topics: Acrylamide; Acrylamides; Adenine Nucleotides; Adenosine Triphosphate; Animals; Annexin A6; Azides; Binding Sites; Energy Metabolism; Humans; In Vitro Techniques; Liver; Nucleotides; Protein Conformation; Spectrometry, Fluorescence; Swine; Tryptophan | 1997 |
Intrinsic fluorescence in endoglucanase and cellobiohydrolase from Trichoderma pseudokiningii S-38: effects of pH, quenching agents, and ligand binding.
Topics: Acrylamide; Acrylamides; Cellulase; Cellulose 1,4-beta-Cellobiosidase; Fluorescence; Hydrogen-Ion Concentration; Kinetics; Ligands; Potassium Iodide; Spectrometry, Fluorescence; Substrate Specificity; Trichoderma; Tryptophan | 1997 |
Modifications induced by insulin-dependent diabetes mellitus on human placental Na+/K+-adenosine triphosphatase.
Topics: Acrylamide; Acrylamides; Adult; Anthracenes; Binding Sites; Diabetes Mellitus, Type 1; Diffusion; Diphenylhexatriene; Enzyme Activation; Female; Fluorescence Polarization; Fluorescent Dyes; Humans; Isothiocyanates; Kinetics; Membrane Fluidity; Microsomes; Ouabain; Placenta; Pregnancy; Pregnancy in Diabetics; Sodium-Potassium-Exchanging ATPase; Spectrometry, Fluorescence; Tryptophan | 1997 |
Mapping the suramin-binding sites of human neutrophil elastase: investigation by fluorescence resonance energy transfer and molecular modeling.
Topics: Acrylamide; Acrylamides; Amino Acid Chloromethyl Ketones; Binding Sites; Energy Transfer; Enzyme Inhibitors; Humans; Iodides; Kinetics; Leukocyte Elastase; Models, Molecular; Neutrophils; Protein Binding; Protein Conformation; Spectrometry, Fluorescence; Spectrophotometry; Suramin; Tryptophan | 1997 |
Functional implications of disulfide bond, Cys45-Cys50, in recombinant prochymosin.
Topics: Acrylamide; Acrylamides; Animals; Caseins; Cattle; Chymosin; Circular Dichroism; Cysteine; Disulfides; Enzyme Activation; Enzyme Precursors; Enzyme Stability; Escherichia coli; Kinetics; Mutagenesis, Site-Directed; Protein Conformation; Protein Denaturation; Protein Folding; Recombinant Proteins; Spectrometry, Fluorescence; Substrate Specificity; Tryptophan; Urea | 1997 |
Analysis of the conformational stability of the active domain of recombinant mouse TIMP-1 by intrinsic fluorescence.
Topics: Acrylamide; Acrylamides; Animals; Binding Sites; Guanidine; Mice; Protein Conformation; Protein Denaturation; Protein Folding; Recombinant Proteins; Spectrometry, Fluorescence; Temperature; Thermodynamics; Tissue Inhibitor of Metalloproteinase-1; Tryptophan | 1998 |
A collapsed intermediate with nonnative packing of hydrophobic residues in the folding of TEM-1 beta-lactamase.
Topics: Acrylamide; Acrylamides; Anilino Naphthalenesulfonates; Bacterial Proteins; beta-Lactamases; Circular Dichroism; Escherichia coli; Fluorescence Polarization; Kinetics; Protein Folding; Spectrometry, Fluorescence; Tryptophan | 1998 |
Spectroscopic analysis of halothane binding to the plasma membrane Ca2+-ATPase.
Topics: Acrylamide; Acrylamides; Anesthetics, Inhalation; Binding Sites; Calcium-Transporting ATPases; Ethers; Halothane; Kinetics; Phosphatidylcholines; Protein Conformation; Protein Denaturation; Spectrometry, Fluorescence; Tryptophan; Urea | 1998 |
Steady state and time-resolved fluorescence study of residual structures in an unfolded form of yeast phosphoglycerate kinase.
Topics: Acrylamide; Acrylamides; Cold Temperature; Diffusion; Guanidine; Kinetics; Mutagenesis, Site-Directed; Phenylalanine; Phosphoglycerate Kinase; Protein Conformation; Protein Denaturation; Protein Folding; Saccharomyces cerevisiae; Solvents; Spectrometry, Fluorescence; Tryptophan; Tyrosine; Viscosity | 1998 |
The orientation of nisin in membranes.
Topics: Acrylamide; Acrylamides; Amino Acid Sequence; Lipid Bilayers; Models, Molecular; Molecular Sequence Data; Mutagenesis, Site-Directed; Nisin; Phosphatidylcholines; Phosphatidylglycerols; Spectrometry, Fluorescence; Spin Labels; Tryptophan | 1998 |
Effects of metal binding affinity on the chemical and thermal stability of site-directed mutants of rat oncomodulin.
Topics: Acrylamide; Acrylamides; Animals; Calcium; Calcium-Binding Proteins; Fluorescence; Guanidine; Hydrogen Bonding; Metals; Mutagenesis, Site-Directed; Mutation; Neoplasm Proteins; Protein Binding; Protein Denaturation; Protein Folding; Protein Structure, Secondary; Rats; Temperature; Terbium; Thermodynamics; Tryptophan | 1998 |
Effect of single mutations on the structural dynamics of a DNA repair enzyme, the Escherichia coli formamidopyrimidine-DNA glycosylase--a fluorescence study using tryptophan residues as reporter groups.
Topics: Acrylamide; Acrylamides; Bacterial Proteins; DNA Ligases; DNA-Formamidopyrimidine Glycosylase; Escherichia coli; Escherichia coli Proteins; N-Glycosyl Hydrolases; Point Mutation; Protein Conformation; Spectrometry, Fluorescence; Tryptophan | 1998 |
Time-resolved and steady-state fluorescence quenching of N-acetyl-L-tryptophanamide by acrylamide and iodide.
Topics: Acrylamide; Acrylamides; Fluorescence; Mathematical Computing; Propylene Glycol; Proteins; Spectrometry, Fluorescence; Time Factors; Tryptophan; Viscosity | 1998 |
Spectroscopic properties and stability of the neurotoxic complex. Vipoxin and its components.
Topics: Acrylamide; Acrylamides; Drug Stability; Energy Transfer; Enzyme Inhibitors; Guanidine; Kinetics; Models, Molecular; Neurotoxins; Phospholipases A; Phospholipases A2; Protein Conformation; Spectrometry, Fluorescence; Tryptophan; Tyrosine; Viper Venoms | 1998 |
Conformational flexibility of domain III of annexin V studied by fluorescence of tryptophan 187 and circular dichroism: the effect of pH.
Topics: Acrylamide; Acrylamides; Annexin A5; Circular Dichroism; Fluorescence Polarization; Humans; Hydrogen-Ion Concentration; Protein Conformation; Protein Structure, Secondary; Protein Structure, Tertiary; Solubility; Spectrometry, Fluorescence; Thermodynamics; Tryptophan; Water | 1998 |
Modification of sticholysin II hemolytic activity by free radicals.
Topics: Acrylamide; Amidines; Animals; Cnidarian Venoms; Erythrocytes; Fluorescence; Free Radicals; Hemolysin Proteins; Humans; Oxidants; Sea Anemones; Sialyltransferases; Tryptophan | 1998 |
Some aspects of beta-lactoglobulin structural properties in solution studied by fluorescence quenching.
Topics: Acrylamide; Binding Sites; Fluorescence; Kinetics; Lactoglobulins; Palmitic Acid; Protein Conformation; Protein Denaturation; Sodium Nitrite; Solutions; Spectrometry, Fluorescence; Tryptophan; Urea | 1998 |
Fluorescence quenching and time-resolved fluorescence studies on Momordica charantia (bitter gourd) seed lectin.
Topics: Acrylamide; Cesium; Fluorescence; Iodides; Lactose; Lectins; N-Glycosyl Hydrolases; Plant Proteins; Protein Denaturation; Ribosome Inactivating Proteins, Type 2; Spectrometry, Fluorescence; Tryptophan; Urea | 1998 |
Purification and characterization of papaya glutamine cyclotransferase, a plant enzyme highly resistant to chemical, acid and thermal denaturation.
Topics: Acrylamide; Aminoacyltransferases; Cysteine Endopeptidases; Glycoproteins; Guanidine; Hydrogen-Ion Concentration; Latex; Plant Proteins; Protein Denaturation; Protein Folding; Spectrometry, Fluorescence; Temperature; Tryptophan | 1998 |
Conformational changes of the leukocyte NADPH oxidase subunit p47(phox) during activation studied through its intrinsic fluorescence.
Topics: Acrylamide; Anions; Binding Sites; Cytochrome b Group; Enzyme Activation; Fatty Acids; Fluorescence; NADPH Oxidases; Neutrophils; Phosphoproteins; Phosphorylation; Protein Conformation; Protein Kinase C; Recombinant Proteins; Sodium Dodecyl Sulfate; Surface-Active Agents; Tryptophan | 1998 |
Tryptophan fluorescence of the lux-specific Vibrio harveyi acyl-ACP thioesterase and its tryptophan mutants: structural properties and ligand-induced conformational change.
Topics: Acrylamide; Acyltransferases; Bacterial Proteins; Kinetics; Ligands; Mutagenesis, Site-Directed; Protein Conformation; Recombinant Proteins; Spectrometry, Fluorescence; Substrate Specificity; Thiolester Hydrolases; Tryptophan; Tyrosine; Vibrio | 1998 |
Inhibition of myosin ATPase by metal fluoride complexes.
Topics: Acrylamide; Actins; Adenosine Diphosphate; Binding Sites; Circular Dichroism; Enzyme Inhibitors; Fluorescence; Fluorides; Gallium; Magnesium Compounds; Myosins; Tryptophan | 1999 |
Ligand-dependent conformational equilibria of serum albumin revealed by tryptophan fluorescence quenching.
Topics: Acrylamide; Biophysical Phenomena; Biophysics; Chromatography, Gel; Fluorescence Polarization; Humans; In Vitro Techniques; Iodides; Kinetics; Ligands; Oleic Acid; Protein Conformation; Serum Albumin; Spectrometry, Fluorescence; Tryptophan | 1999 |
Resolution and characterization of tryptophyl fluorescence of hen egg-white lysozyme by quenching- and time-resolved spectroscopy.
Topics: Acetylglucosamine; Acrylamide; Animals; Chickens; Chromatography, Ion Exchange; Female; Kinetics; Muramidase; Mutagenesis, Site-Directed; Protein Conformation; Spectrometry, Fluorescence; Tryptophan | 1999 |
Amphipathic alpha-helix bundle organization of lipid-free chicken apolipoprotein A-I.
Topics: Acrylamide; Anilino Naphthalenesulfonates; Animals; Apolipoprotein A-I; Cesium; Chickens; Chlorides; Circular Dichroism; Fatty Acids; Fluorescence Polarization; Fluorescent Dyes; Humans; Lipids; Potassium Iodide; Protein Structure, Secondary; Spectrometry, Fluorescence; Spin Labels; Trifluoroethanol; Tryptophan | 1999 |
Time-resolved distance determination by tryptophan fluorescence quenching: probing intermediates in membrane protein folding.
Topics: Acrylamide; Bacterial Outer Membrane Proteins; Biological Transport; Escherichia coli; Fluorescence Polarization; Lipid Bilayers; Normal Distribution; Protein Folding; Spectrometry, Fluorescence; Temperature; Time Factors; Tryptophan | 1999 |
Molecular cloning and expression of adenosine kinase from Leishmania donovani: identification of unconventional P-loop motif.
Topics: Acrylamide; Adenosine Kinase; Adenosine Triphosphate; Amino Acid Sequence; Animals; Base Sequence; Binding Sites; Cloning, Molecular; Consensus Sequence; Escherichia coli; Fluorescence; Humans; Kinetics; Leishmania donovani; Molecular Sequence Data; Molecular Weight; Open Reading Frames; Potassium Iodide; Recombinant Proteins; RNA, Messenger; Sequence Homology, Amino Acid; Trans-Splicing; Tryptophan | 1999 |
The structure and dynamics of partially folded actin.
Topics: Acrylamide; Actins; Models, Molecular; Protein Conformation; Protein Denaturation; Protein Folding; Spectrometry, Fluorescence; Temperature; Tryptophan; Urea | 1999 |
Effect of acrylamide on aldolase structure. I. Induction of intermediate states.
Topics: Acrylamide; Animals; Binding Sites; Circular Dichroism; Fructose-Bisphosphate Aldolase; Kinetics; Muscles; Protein Conformation; Rabbits; Spectrometry, Fluorescence; Tryptophan | 1999 |
Effect of acrylamide on aldolase structure. II. Characterization of aldolase unfolding intermediates.
Topics: Acrylamide; Dose-Response Relationship, Drug; Fructose-Bisphosphate Aldolase; Light; Models, Molecular; Protein Conformation; Protein Folding; Scattering, Radiation; Spectrometry, Fluorescence; Sulfhydryl Compounds; Tryptophan | 1999 |
Ligand-mediated tertiary structure changes of reconstituted P-glycoprotein. A tryptophan fluorescence quenching analysis.
Topics: Acrylamide; Adenosine Triphosphate; Affinity Labels; Animals; Antibiotics, Antineoplastic; ATP Binding Cassette Transporter, Subfamily B, Member 1; Azides; CHO Cells; Cricetinae; Dihydropyridines; Drug Resistance, Multiple; Fluorescence; Ligands; Nucleotides; Protein Binding; Protein Conformation; Protein Structure, Tertiary; Proteolipids; Tryptophan | 1999 |
Tryptophan microstate reshuffling upon the binding of cyclosporin A to human cyclophilin A.
Topics: Acrylamide; Amino Acid Substitution; Base Sequence; Cyclosporine; DNA Primers; Humans; Molecular Sequence Data; Peptidylprolyl Isomerase; Spectrometry, Fluorescence; Tryptophan | 1999 |
Adventures in membrane protein topology. A study of the membrane-bound state of colicin E1.
Topics: Acrylamide; Amino Acid Sequence; Colicins; Fluorescent Dyes; Ion Channels; Liposomes; Membrane Proteins; Models, Molecular; Molecular Sequence Data; Mutation; Naphthalenes; Peptide Fragments; Protein Conformation; Protein Structure, Secondary; Spectrometry, Fluorescence; Tryptophan | 1999 |
Optical spectroscopic characterization of single tryptophan mutants of chicken skeletal troponin C: evidence for interdomain interaction.
Topics: Acrylamide; Animals; Calcium; Chickens; Circular Dichroism; Muscle, Skeletal; Mutagenesis, Site-Directed; Peptide Fragments; Potassium Iodide; Protein Structure, Secondary; Spectrometry, Fluorescence; Spectrophotometry, Ultraviolet; Temperature; Titrimetry; Troponin C; Tryptophan | 1999 |
The molten globule state of a chimera of human alpha-lactalbumin and equine lysozyme.
Topics: Acrylamide; Amino Acid Sequence; Anilino Naphthalenesulfonates; Animals; Circular Dichroism; Enzyme Stability; Fluorescent Dyes; Guanidine; Horses; Humans; Hydrogen-Ion Concentration; Lactalbumin; Molecular Sequence Data; Muramidase; Protein Denaturation; Protein Folding; Protein Structure, Secondary; Recombinant Fusion Proteins; Solvents; Spectrometry, Fluorescence; Thermodynamics; Tryptophan | 1999 |
Effect of self-association on the structural organization of partially folded proteins: inactivated actin.
Topics: Acrylamide; Actins; Anilino Naphthalenesulfonates; Animals; Guanidine; Protein Conformation; Protein Folding; Protein Multimerization; Protein Unfolding; Rabbits; Solvents; Spectrometry, Fluorescence; Spectrophotometry, Ultraviolet; Tryptophan | 1999 |
Role of the C-terminal helix 9 in the stability and ligandin function of class alpha glutathione transferase A1-1.
Topics: Acrylamide; Anilino Naphthalenesulfonates; Binding Sites; Enzyme Stability; Fluorescent Dyes; Glutathione Transferase; Humans; Isoenzymes; Kinetics; Ligands; Mutagenesis, Site-Directed; Peptide Fragments; Phenylalanine; Protein Folding; Protein Structure, Secondary; Protein Structure, Tertiary; Sequence Deletion; Spectrometry, Fluorescence; Sulfobromophthalein; Tryptophan | 1999 |
Fluorescence and circular dichroism spectroscopic studies on bovine lactoperoxidase.
Topics: Acrylamide; Animals; Cattle; Cesium; Circular Dichroism; Fluorescent Dyes; Guanidine; Iodine; Lactoperoxidase; Milk; Spectrometry, Fluorescence; Tryptophan; Urea | 1999 |
Folding and assembly of dimeric human glutathione transferase A1-1.
Topics: Acrylamide; Anilino Naphthalenesulfonates; Binding Sites; Chromatography, High Pressure Liquid; Circular Dichroism; Dimerization; Enzyme Activation; Fluorescent Dyes; Glutathione Transferase; Humans; Isoenzymes; Protein Folding; Protein Structure, Secondary; Protein Structure, Tertiary; Spectrometry, Fluorescence; Stereoisomerism; Thermodynamics; Tryptophan | 1999 |
Structure-function studies of tryptophan mutants of equinatoxin II, a sea anemone pore-forming protein.
Topics: Acrylamide; Amino Acid Sequence; Animals; Bromosuccinimide; Cattle; Cell Death; Cnidarian Venoms; Cytotoxins; Erythrocytes; Fluorescence; Hemolysis; Liposomes; Molecular Sequence Data; Mutation; Nitrogen; Porins; Protein Structure, Secondary; Recombinant Proteins; Sea Anemones; Spectroscopy, Fourier Transform Infrared; Structure-Activity Relationship; Tryptophan; Water | 2000 |
Acrylamide quenching of apo- and holo-alpha-lactalbumin in guanidine hydrochloride.
Topics: Acrylamide; Animals; Apoproteins; Cattle; Guanidine; Kinetics; Lactalbumin; Protein Denaturation; Protein Folding; Protein Structure, Tertiary; Spectrometry, Fluorescence; Tryptophan | 2000 |
DNA sequence dependent and independent conformational changes in multipartite operator recognition by lambda-repressor.
Topics: Acrylamide; Anilino Naphthalenesulfonates; Bacteriophage lambda; Base Sequence; Binding Sites; Circular Dichroism; DNA-Binding Proteins; Fluorescent Dyes; Operator Regions, Genetic; Protein Binding; Protein Conformation; Repressor Proteins; Spectrometry, Fluorescence; Tryptophan; Viral Proteins; Viral Regulatory and Accessory Proteins | 2000 |
Features of F(1)-ATPase catalytic and noncatalytic sites revealed by fluorescence lifetimes and acrylamide quenching of specifically inserted tryptophan residues.
Topics: Acrylamide; Binding Sites; Escherichia coli; Kinetics; Magnesium; Models, Molecular; Mutation; Nucleotides; Protein Binding; Protein Conformation; Proton-Translocating ATPases; Spectrometry, Fluorescence; Tryptophan | 2000 |
Lipid binding of the exchangeable apolipoprotein apolipophorin III induces major changes in fluorescence properties of tryptophans 115 and 130.
Topics: Acrylamide; Animals; Apolipoproteins; Circular Dichroism; Dimyristoylphosphatidylcholine; Grasshoppers; Guanidine; Insect Proteins; Lipids; Mutation; Nephelometry and Turbidimetry; Protein Binding; Protein Structure, Secondary; Spectrometry, Fluorescence; Trypsin; Tryptophan | 2000 |
Spectroscopic characterization of the interaction between calmodulin-dependent protein kinase I and calmodulin.
Topics: Acrylamide; Amino Acid Sequence; Binding Sites; Calcium-Calmodulin-Dependent Protein Kinase Type 1; Calcium-Calmodulin-Dependent Protein Kinases; Calmodulin; Circular Dichroism; Electrophoresis, Polyacrylamide Gel; Methionine; Molecular Sequence Data; Mutation; Peptide Fragments; Protein Binding; Protein Conformation; Spectrometry, Fluorescence; Tryptophan | 2000 |
Isolating and localizing ATP-sensitive tryptophan emission in skeletal myosin subfragment 1.
Topics: Acrylamide; Adenosine Triphosphate; Animals; Binding Sites; Models, Chemical; Muscle, Skeletal; Myosin Subfragments; Myosins; Protein Conformation; Quantum Theory; Rabbits; Spectrometry, Fluorescence; Sulfonium Compounds; Tryptophan | 2000 |
Steady state and picosecond time-resolved fluorescence studies on native, desulpho and deflavo xanthine oxidase.
Topics: Acrylamide; Cesium; Chlorides; Potassium Iodide; Protein Conformation; Spectrometry, Fluorescence; Tryptophan; Xanthine Oxidase | 2000 |
Spectroscopic studies on the interaction of bovine (BSA) and human (HSA) serum albumins with ionic surfactants.
Topics: Acrylamide; Animals; Buffers; Cattle; Circular Dichroism; Humans; Myristic Acid; Protein Structure, Secondary; Quaternary Ammonium Compounds; Serum Albumin; Serum Albumin, Bovine; Sodium Dodecyl Sulfate; Spectrometry, Fluorescence; Surface-Active Agents; Tromethamine; Tryptophan | 2000 |
Intrinsic fluorescence of the P-glycoprotein multidrug transporter: sensitivity of tryptophan residues to binding of drugs and nucleotides.
Topics: Acrylamide; Amino Acid Sequence; Animals; ATP Binding Cassette Transporter, Subfamily B, Member 1; Cricetinae; Iodides; Models, Molecular; Molecular Sequence Data; Nucleotides; Peptides; Pharmaceutical Preparations; Spectrometry, Fluorescence; Tryptophan | 2000 |
Trp42 rotamers report reduced flexibility when the inhibitor acetyl-pepstatin is bound to HIV-1 protease.
Topics: Acrylamide; Computer Simulation; Escherichia coli; HIV Protease; Kinetics; Models, Molecular; Mutagenesis; Pepstatins; Protein Binding; Protein Conformation; Spectrometry, Fluorescence; Time Factors; Tryptophan | 2000 |
Calcium- and magnesium-dependent interactions between the C-terminus of troponin I and the N-terminal, regulatory domain of troponin C.
Topics: Acrylamide; Amino Acid Substitution; Animals; Binding Sites; Calcium; Iodides; Magnesium; Muscle Contraction; Mutagenesis, Site-Directed; Protein Structure, Tertiary; Rabbits; Sequence Deletion; Spectrometry, Fluorescence; Troponin C; Troponin I; Tryptophan | 2001 |
Fluorometric and mass spectrometric analysis of nonenzymatic glycosylated albumin.
Topics: Acrylamide; Anilino Naphthalenesulfonates; Binding Sites; Cyanogen Bromide; Fluorescent Dyes; Fluorometry; Glucose; Glycation End Products, Advanced; Glycosylation; Humans; Models, Molecular; Peptide Fragments; Peptide Mapping; Serum Albumin; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Trypsin; Tryptophan | 2001 |
Pseudoreversion of the catalytic activity of Y14F by the additional substitution(s) of tyrosine with phenylalanine in the hydrogen bond network of delta 5-3-ketosteroid isomerase from Pseudomonas putida biotype B.
Topics: Acrylamide; Amino Acid Substitution; Androstenedione; Catalysis; Crystallography, X-Ray; Deuterium Oxide; Enzyme Activation; Hydrogen Bonding; Kinetics; Mutagenesis, Site-Directed; Phenylalanine; Pseudomonas putida; Spectrometry, Fluorescence; Steroid Isomerases; Structure-Activity Relationship; Substrate Specificity; Tryptophan; Tyrosine | 2001 |
On the effect of sodium dodecyl sulfate on the structure of beta-galactosidase from Escherichia coli. A fluorescence study.
Topics: Acrylamide; beta-Galactosidase; Escherichia coli; Fluorescence Polarization; Models, Molecular; Protein Structure, Tertiary; Sodium Dodecyl Sulfate; Spectrometry, Fluorescence; Temperature; Tryptophan | 2001 |
Conformational changes that occur during an RNA-editing adenosine deamination reaction.
Topics: 2-Aminopurine; Acrylamide; Adenosine Deaminase; Animals; Base Sequence; Binding Sites; DNA Primers; Fluorescence; Hydrolysis; Nucleic Acid Conformation; Rats; RNA Editing; Tryptophan | 2001 |
Cofactor and tryptophan accessibility and unfolding of brain glutamate decarboxylase.
Topics: Acrylamide; Animals; Anions; Binding Sites; Brain; Cell Line; Cesium; Circular Dichroism; Dimerization; Dose-Response Relationship, Drug; Glutamate Decarboxylase; Guanidine; Insecta; Iodine; Ions; Kinetics; Protein Binding; Protein Conformation; Protein Denaturation; Protein Folding; Protein Structure, Secondary; Protein Structure, Tertiary; Rats; Spectrometry, Fluorescence; Tryptophan | 2001 |
Conformation of the C-terminal domain of the pro-apoptotic protein Bax and mutants and its interaction with membranes.
Topics: Acrylamide; Amino Acid Sequence; Apoptosis; bcl-2-Associated X Protein; Cell Membrane; Diphenylhexatriene; Gene Deletion; Kinetics; Liposomes; Mitochondria; Molecular Sequence Data; Mutation; Peptides; Protein Binding; Protein Conformation; Protein Structure, Secondary; Protein Structure, Tertiary; Proto-Oncogene Proteins; Proto-Oncogene Proteins c-bcl-2; Spectrometry, Fluorescence; Spectrophotometry; Spectrophotometry, Infrared; Tryptophan | 2001 |
Probing the structural diversities of long alpha-neurotoxins by fluorescence quenching studies.
Topics: Acrylamide; Animals; Bungarus; Colubridae; Computer Simulation; Fluorescence; Iodides; Models, Molecular; Neurotoxins; Protein Conformation; Snake Venoms; Structure-Activity Relationship; Tryptophan | 2001 |
Role of conformational changes in the heme-dependent regulation of human soluble guanylate cyclase.
Topics: Acrylamide; Circular Dichroism; Enzyme Activation; Guanylate Cyclase; Heme; Humans; Indazoles; Models, Chemical; Models, Molecular; Nitric Oxide; Nitric Oxide Donors; Protein Conformation; Protein Structure, Secondary; Spectrometry, Fluorescence; Tryptophan | 2001 |
Binding of prion protein to lipid membranes and implications for prion conversion.
Topics: 1,2-Dipalmitoylphosphatidylcholine; Acrylamide; Animals; Cholesterol; Circular Dichroism; Cricetinae; Fluoresceins; Fluorescence; Fourier Analysis; Hydrogen-Ion Concentration; Hydrophobic and Hydrophilic Interactions; Kinetics; Lipid Bilayers; Liposomes; Membrane Microdomains; Mesocricetus; Models, Molecular; Phosphatidylglycerols; Prions; Protein Binding; Protein Structure, Secondary; Spectroscopy, Fourier Transform Infrared; Sphingomyelins; Static Electricity; Tryptophan | 2002 |
Second derivative fluorescence spectra of indole compounds.
Topics: Acrylamide; Protein Conformation; Protein Denaturation; Protein Folding; Proteins; Spectrometry, Fluorescence; Spectrum Analysis; Tryptophan; Tyrosine | 2002 |
Effect of urea denaturation on tryptophan fluorescence and nucleotide binding on tubulin studied by fluorescence and NMR spectroscopic methods.
Topics: Acrylamide; Animals; Brain; Dose-Response Relationship, Drug; Goats; Guanosine Triphosphate; Hydrogen-Ion Concentration; Magnetic Resonance Spectroscopy; Nucleotides; Protein Binding; Protein Denaturation; Protein Folding; Spectrometry, Fluorescence; Time Factors; Tryptophan; Tubulin; Urea | 2001 |
Effect of heavy water on protein flexibility.
Topics: Acrylamide; Animals; Biophysical Phenomena; Biophysics; Deuterium Oxide; In Vitro Techniques; Kinetics; Luminescence; Protein Conformation; Protein Folding; Proteins; Thermodynamics; Tryptophan | 2002 |
Half-of-the-sites reactivity of F235C lambda-repressor: implications for the structure of the whole repressor.
Topics: Acrylamide; Circular Dichroism; Cysteine; DNA-Binding Proteins; Fluorescent Dyes; Mutagenesis, Site-Directed; Protein Denaturation; Protein Structure, Tertiary; Repressor Proteins; Spectrometry, Fluorescence; Sulfhydryl Compounds; Tryptophan; Urea; Viral Proteins; Viral Regulatory and Accessory Proteins | 2002 |
Unfolding and conformational studies on bovine adrenodoxin probed by engineered intrinsic tryptophan fluorescence.
Topics: Acrylamide; Adrenodoxin; Amino Acid Substitution; Animals; Cattle; Energy Transfer; Hot Temperature; Kinetics; Models, Molecular; Mutagenesis, Site-Directed; Protein Conformation; Protein Denaturation; Protein Folding; Recombinant Proteins; Spectrometry, Fluorescence; Tryptophan | 2002 |
Lipid II induces a transmembrane orientation of the pore-forming peptide lantibiotic nisin.
Topics: Acrylamide; Bacteria; Lipid Metabolism; Nisin; Spectrometry, Fluorescence; Spin Labels; Tryptophan; Uridine Diphosphate N-Acetylmuramic Acid | 2002 |
Fluorescence quenching of dimeric and monomeric forms of yeast hexokinase (PII): effect of substrate binding steady-state and time-resolved fluorescence studies.
Topics: Acrylamide; Crystallography; Dimerization; Enzyme Activation; Glucose; Hexokinase; Isoenzymes; Macromolecular Substances; Molecular Conformation; Potassium Iodide; Spectrometry, Fluorescence; Substrate Specificity; Tryptophan; Yeasts | 2002 |
Differences in nucleotide-binding site of isoapyrases deduced from tryptophan fluorescence.
Topics: Acrylamide; Adenosine Diphosphate; Adenosine Triphosphate; Apyrase; Binding Sites; Cesium; Iodides; Isoenzymes; Kinetics; Nucleotides; Photobleaching; Solanum tuberosum; Spectrometry, Fluorescence; Substrate Specificity; Tryptophan | 2003 |
Inter-subunit recognition and manifestation of segmental mobility in Escherichia coli RNA polymerase: a case study with omega-beta' interaction.
Topics: Acrylamide; Amino Acid Sequence; Cysteine; DNA-Directed RNA Polymerases; Escherichia coli; Fluorescence Polarization; Models, Molecular; Molecular Sequence Data; Mutagenesis, Site-Directed; Naphthalenesulfonates; Protein Denaturation; Protein Folding; Protein Renaturation; Protein Structure, Secondary; Protein Subunits; Spectrometry, Fluorescence; Thermus; Tryptophan | 2003 |
Surface-exposed tryptophan residues are essential for O-acetylserine sulfhydrylase structure, function, and stability.
Topics: Acrylamide; Binding Sites; Circular Dichroism; Cysteine Synthase; Enzyme Stability; Fluorescence; Protein Denaturation; Protein Folding; Protein Structure, Secondary; Structure-Activity Relationship; Tryptophan | 2003 |
Examination of EmrE conformational differences in various membrane mimetic environments.
Topics: Acrylamide; Amino Acid Sequence; Antiporters; Cell Membrane; Circular Dichroism; Drug Resistance, Multiple, Bacterial; Escherichia coli; Escherichia coli Proteins; Fluorescence Polarization; Humans; Membrane Proteins; Molecular Sequence Data; Protein Conformation; Protein Structure, Secondary; Sodium Dodecyl Sulfate; Solubility; Spectrometry, Fluorescence; Tryptophan | 2003 |
The dead-end elimination method, tryptophan rotamers, and fluorescence lifetimes.
Topics: Acrylamide; Algorithms; Biophysics; Crystallography, X-Ray; Dose-Response Relationship, Drug; Electrons; Fluorescence; Hydrogen; Kinetics; Models, Statistical; Molecular Conformation; Mutation; Protein Conformation; Spectrometry, Fluorescence; Time Factors; Trichosanthes; Tryptophan | 2003 |
Tryptophan phosphorescence spectroscopy reveals that a domain in the NAD(H)-binding component (dI) of transhydrogenase from Rhodospirillum rubrum has an extremely rigid and conformationally homogeneous protein core.
Topics: Acrylamide; Dimerization; Escherichia coli; Glycerol; Kinetics; Luminescent Measurements; Models, Molecular; NAD; NADP; NADP Transhydrogenases; Oxidation-Reduction; Protein Binding; Protein Conformation; Protein Structure, Tertiary; Protons; Rhodospirillum rubrum; Spectrometry, Fluorescence; Spectrophotometry; Temperature; Time Factors; Tryptophan | 2003 |
C-terminal functional unit of Rapana thomasiana (marine snail, gastropod) hemocyanin isoform RtH1: isolation and characterization.
Topics: Acrylamide; Amino Acids; Animals; Hemocyanins; Hydrogen-Ion Concentration; Peptide Fragments; Protein Conformation; Protein Isoforms; Protein Subunits; Seawater; Snails; Temperature; Tryptophan; Tyrosine | 2003 |
The single tryptophan of the PsbQ protein of photosystem II is at the end of a 4-alpha-helical bundle domain.
Topics: Acrylamide; Cesium; Imaging, Three-Dimensional; Iodides; Models, Molecular; Photosynthetic Reaction Center Complex Proteins; Photosystem II Protein Complex; Protein Conformation; Protein Folding; Protein Structure, Tertiary; Spectrometry, Fluorescence; Spectrum Analysis; Structural Homology, Protein; Tryptophan | 2003 |
A study of tryptophan fluorescence quenching of bifunctional alginate lyase from a marine bacterium Pseudoalteromonas sp. strain No. 272 by acrylamide.
Topics: Acrylamide; Methylation; Oligosaccharides; Polysaccharide-Lyases; Pseudoalteromonas; Spectrometry, Fluorescence; Substrate Specificity; Tryptophan | 2003 |
Steady-state kinetics and tryptophan fluorescence properties of halohydrin dehalogenase from Agrobacterium radiobacter. Roles of W139 and W249 in the active site and halide-induced conformational change.
Topics: Acrylamide; Amino Acid Sequence; Bacterial Proteins; Binding Sites; Bromides; Catalysis; Hydrogen-Ion Concentration; Hydrolases; Kinetics; Molecular Sequence Data; Mutagenesis, Site-Directed; Phenylalanine; Protein Binding; Protein Conformation; Rhizobium; Spectrometry, Fluorescence; Stereoisomerism; Substrate Specificity; Tryptophan | 2003 |
Increase in molecular rigidity of the protein conformation of brain Na+-K+-ATPase by modification with 4-hydroxy-2-nonenal.
Topics: Acrylamide; Aldehydes; Animals; Anisotropy; Brain; Dose-Response Relationship, Drug; Ethylmaleimide; Fluorescence; Guanidine; Lipid Peroxidation; Molecular Conformation; Protein Conformation; Sodium-Potassium-Exchanging ATPase; Sulfhydryl Compounds; Swine; Tryptophan | 2003 |
Tryptophanyl substitutions in apomyoglobin affect conformation and dynamic properties of AGH subdomain.
Topics: Acrylamide; Amino Acid Substitution; Animals; Apoproteins; Horses; Iodides; Myoglobin; Protein Conformation; Spectrometry, Fluorescence; Tryptophan; Whales | 2003 |
Hypochlorite action on plasma fibronectin promotes its extended conformation in complexes with antibodies.
Topics: Acrylamide; Antibodies; Antigen-Antibody Complex; Chlorine; Enzyme-Linked Immunosorbent Assay; Fibronectins; Fluorescence; Humans; Hypochlorous Acid; Luminescent Measurements; Tryptophan; Tyrosine | 2003 |
Tryptophan spectroscopy studies and black lipid bilayer analysis indicate that the oligomeric structure of Cry1Ab toxin from Bacillus thuringiensis is the membrane-insertion intermediate.
Topics: Acrylamide; Bacillus thuringiensis; Bacillus thuringiensis Toxins; Bacterial Proteins; Bacterial Toxins; Endotoxins; Hemolysin Proteins; Ion Channels; Lipid Bilayers; Liposomes; Membrane Proteins; Phosphatidylcholines; Phospholipids; Solutions; Spectrometry, Fluorescence; Tryptophan; Water | 2004 |
Effects of L- or D-Pro incorporation into hydrophobic or hydrophilic helix face of amphipathic alpha-helical model peptide on structure and cell selectivity.
Topics: Acrylamide; Biochemistry; Circular Dichroism; Dose-Response Relationship, Drug; Escherichia coli; Kinetics; Lipids; Peptide Biosynthesis; Peptides; Proline; Spectrometry, Fluorescence; Tryptophan | 2004 |
Systematic peptide engineering and structural characterization to search for the shortest antimicrobial peptide analogue of gaegurin 5.
Topics: Acrylamide; Amino Acid Sequence; Amino Acids; Anti-Bacterial Agents; Antimicrobial Cationic Peptides; Bacteria; Binding Sites; Circular Dichroism; Dose-Response Relationship, Drug; Drug Design; Erythrocytes; Hemolysis; Humans; Kinetics; Magnetic Resonance Spectroscopy; Micelles; Models, Molecular; Molecular Sequence Data; Peptides; Protein Conformation; Protein Engineering; Protein Precursors; Protein Structure, Tertiary; Sequence Homology, Amino Acid; Spectrometry, Fluorescence; Spectrophotometry; Time Factors; Tryptophan | 2004 |
Effects of cavity-forming mutations on the internal dynamics of azurin.
Topics: Acrylamide; Amino Acid Substitution; Azurin; Mutation; Porosity; Protein Conformation; Protein Folding; Protein Structure, Tertiary; Structure-Activity Relationship; Temperature; Tryptophan | 2004 |
Conformational changes of beta-lactoglobulin in sodium bis(2-ethylhexyl) sulfosuccinate reverse micelles. A fluorescence and CD study.
Topics: Acrylamide; Animals; Cattle; Circular Dichroism; Dioctyl Sulfosuccinic Acid; Kinetics; Lactoglobulins; Micelles; Models, Molecular; Protein Conformation; Spectrometry, Fluorescence; Tryptophan; Water | 2004 |
Insight into the activation mechanism of Bordetella pertussis adenylate cyclase by calmodulin using fluorescence spectroscopy.
Topics: 2-Naphthylamine; Acrylamide; Adenosine Triphosphate; Adenylyl Cyclases; Amino Acid Substitution; Bordetella pertussis; Calmodulin; Catalytic Domain; Entropy; Enzyme Activation; Fluorescence Polarization; Kinetics; Models, Chemical; Molecular Weight; ortho-Aminobenzoates; Protein Binding; Recombinant Proteins; Spectrometry, Fluorescence; Tryptophan | 2004 |
Role of hydration in the conformational transitions between unliganded and liganded forms of loop 13 of the Na+/glucose cotransporter 1.
Topics: Acrylamide; Anilino Naphthalenesulfonates; Circular Dichroism; Glycerol; Hydrophobic and Hydrophilic Interactions; Kinetics; Ligands; Membrane Glycoproteins; Monosaccharide Transport Proteins; Phlorhizin; Protein Binding; Protein Conformation; Protein Denaturation; Sodium-Glucose Transporter 1; Spectrometry, Fluorescence; Thermodynamics; Tryptophan; Urea; Water | 2004 |
Membrane binding and translocation of cell-penetrating peptides.
Topics: Acrylamide; Amino Acid Sequence; Bromine; Carrier Proteins; Cell Membrane Permeability; Cell-Penetrating Peptides; Coumarins; Fluorescence Resonance Energy Transfer; Gene Products, tat; Lipid Bilayers; Lysophospholipids; Membrane Microdomains; Molecular Sequence Data; Oligonucleotides; Peptides; Phosphatidylcholines; Phosphatidylglycerols; Protein Binding; Protein Conformation; Protein Transport; Spectrometry, Fluorescence; Tryptophan | 2004 |
HIV Rev self-assembly is linked to a molten-globule to compact structural transition.
Topics: Acrylamide; Binding Sites; Circular Dichroism; Cloning, Molecular; Escherichia coli; Gene Products, rev; HIV-1; Hydrogen-Ion Concentration; Hydrophobic and Hydrophilic Interactions; Protein Denaturation; Protein Folding; rev Gene Products, Human Immunodeficiency Virus; Sodium Chloride; Spectrometry, Fluorescence; Thermodynamics; Tryptophan | 2004 |
Molten globule-like folding intermediate of asialofetuin at acidic pH.
Topics: Acids; Acrylamide; alpha-Fetoproteins; Animals; Asialoglycoproteins; Cattle; Circular Dichroism; Fetuins; Glycosylation; Hydrogen-Ion Concentration; Protein Conformation; Protein Folding; Spectrometry, Fluorescence; Temperature; Tryptophan | 2004 |
Tryptophan fluorescence monitors structural changes accompanying signalling state formation in the photocycle of photoactive yellow protein.
Topics: Acrylamide; Bacterial Proteins; Cloning, Molecular; Darkness; Escherichia coli; Halorhodospira halophila; Kinetics; Light; Luminescent Proteins; Recombinant Proteins; Signal Transduction; Spectrometry, Fluorescence; Tryptophan | 2004 |
C-terminus loop 13 of Na+ glucose cotransporter SGLT1 contains a binding site for alkyl glucosides.
Topics: Acrylamide; Amino Acid Sequence; Binding Sites; Binding, Competitive; Glucose; Glucosides; Hexanols; Ligands; Membrane Glycoproteins; Molecular Sequence Data; Monosaccharide Transport Proteins; Mutagenesis, Site-Directed; Peptide Fragments; Photoaffinity Labels; Protein Structure, Secondary; Sodium; Sodium-Glucose Transporter 1; Spectrometry, Fluorescence; Stereoisomerism; Tryptophan | 2004 |
Conformational changes in azurin from Pseudomona aeruginosa induced through chemical and physical protocols.
Topics: Acrylamide; Azurin; Biophysical Phenomena; Biophysics; Copper; Gadolinium; Guanidine; Hot Temperature; Ligands; Phase Transition; Protein Conformation; Protein Folding; Pseudomonas aeruginosa; Spectrometry, Fluorescence; Temperature; Thermodynamics; Time Factors; Tryptophan | 2004 |
Identification and structural ramifications of a hinge domain in apolipoprotein A-I discoidal high-density lipoproteins of different size.
Topics: Acrylamide; Amino Acid Sequence; Animals; Apolipoprotein A-I; Humans; Lipoproteins, HDL; Models, Molecular; Molecular Sequence Data; Mutation; Particle Size; Protein Conformation; Spin Labels; Tryptophan | 2004 |
Proton motive force mediates a reorientation of the cytosolic domains of the multidrug transporter LmrP.
Topics: Acrylamide; Aspartic Acid; Bacterial Proteins; Benzimidazoles; Biological Transport; Cell Membrane; Cytosol; Dose-Response Relationship, Drug; Drug Resistance, Multiple; Hydrogen-Ion Concentration; Lactococcus lactis; Ligands; Liposomes; Membrane Transport Proteins; Protein Binding; Protein Structure, Tertiary; Proteolipids; Protons; Sepharose; Spectroscopy, Fourier Transform Infrared; Tetracycline; Time Factors; Tryptophan | 2004 |
Comparative studies on the structure and stability of fluorescent proteins EGFP, zFP506, mRFP1, "dimer2", and DsRed1.
Topics: Acrylamide; Amino Acid Sequence; Circular Dichroism; Dose-Response Relationship, Drug; Escherichia coli; Fluorescence Resonance Energy Transfer; Fluorescent Dyes; Gadolinium; Green Fluorescent Proteins; Kinetics; Luminescent Proteins; Molecular Sequence Data; Plasmids; Protein Binding; Protein Conformation; Protein Denaturation; Protein Structure, Quaternary; Recombinant Proteins; Red Fluorescent Protein; Sequence Homology, Amino Acid; Spectrometry, Fluorescence; Spectroscopy, Fourier Transform Infrared; Structure-Activity Relationship; Tryptophan; Ultraviolet Rays | 2004 |
Agonist-mediated conformational changes in acetylcholine-binding protein revealed by simulation and intrinsic tryptophan fluorescence.
Topics: Acetylcholine; Acrylamide; Animals; Binding Sites; Carrier Proteins; Crystallography, X-Ray; Humans; Ligands; Models, Molecular; Protein Binding; Protein Conformation; Protein Structure, Tertiary; Snails; Spectrometry, Fluorescence; Time Factors; Tryptophan | 2005 |
Effect of amino acid residue and oligosaccharide chain chemical modifications on spectral and hemagglutinating activity of Millettia dielsiana Harms. ex Diels. lectin.
Topics: Acrylamide; Amino Acids; Arginine; Binding Sites; Carbohydrates; Circular Dichroism; Hemagglutination; Mannose; Millettia; Oligosaccharides; Oxygen; Plant Lectins; Protein Conformation; Spectrometry, Fluorescence; Spectrophotometry; Tryptophan; Ultraviolet Rays | 2005 |
Structural origin of endotoxin neutralization and antimicrobial activity of a lactoferrin-based peptide.
Topics: Acrylamide; Amino Acid Motifs; Anti-Infective Agents; Antimicrobial Cationic Peptides; Cell Differentiation; Dose-Response Relationship, Drug; Endotoxins; Humans; Lactoferrin; Lipopolysaccharides; Magnetic Resonance Spectroscopy; Micelles; Models, Chemical; Models, Molecular; Peptides; Phosphates; Protein Binding; Protein Conformation; Protein Folding; Protein Structure, Tertiary; Sodium Dodecyl Sulfate; Spectrometry, Fluorescence; Static Electricity; Teichoic Acids; Tryptophan | 2005 |
TBADH activity in water-miscible organic solvents: correlations between enzyme performance, enantioselectivity and protein structure through spectroscopic studies.
Topics: Acetonitriles; Acrylamide; Alcohol Dehydrogenase; Circular Dichroism; Ethanol; Methanol; Oxidation-Reduction; Pentanones; Protein Conformation; Protein Structure, Quaternary; Protein Structure, Secondary; Protein Subunits; Spectrometry, Fluorescence; Stereoisomerism; Thermoanaerobacter; Tryptophan; Water | 2005 |
Dimeric transthyretin variant assembles into spherical neurotoxins.
Topics: Acrylamide; Amino Acid Substitution; Anilino Naphthalenesulfonates; Cell Death; Cell Line, Tumor; Chromatography, Gel; Dimerization; Genetic Variation; Humans; Isoleucine; Microscopy, Atomic Force; Nephelometry and Turbidimetry; Neuroblastoma; Neurotoxins; Prealbumin; Protein Binding; Protein Conformation; Protein Processing, Post-Translational; Protein Structure, Quaternary; Protein Structure, Secondary; Protein Structure, Tertiary; Serine; Spectrometry, Fluorescence; Thermodynamics; Tryptophan | 2005 |
The characterization of plasma membrane Ca2+-ATPase in rich sphingomyelin-cholesterol domains.
Topics: Acrylamide; Calcium-Transporting ATPases; Cell Membrane; Cholesterol; Fluorescence; Phase Transition; Phosphatidylcholines; Proteolipids; Spectrometry, Fluorescence; Sphingomyelins; Tryptophan | 2005 |
Conformational state of the SecYEG-bound SecA probed by single tryptophan fluorescence spectroscopy.
Topics: Acrylamide; Adenosine Triphosphatases; Amino Acid Substitution; Bacterial Proteins; Escherichia coli Proteins; Genetic Complementation Test; Membrane Proteins; Membrane Transport Proteins; Protein Binding; Protein Conformation; Protein Transport; SEC Translocation Channels; SecA Proteins; Solubility; Spectrometry, Fluorescence; Temperature; Tryptophan | 2005 |
Structural rearrangements in the active site of smooth-muscle myosin.
Topics: Acrylamide; Actins; Adenosine Diphosphate; Adenosine Triphosphatases; Adenosine Triphosphate; Animals; Binding Sites; Cell Line; Chickens; Crystallography, X-Ray; Dictyostelium; DNA, Complementary; Dose-Response Relationship, Drug; Fluorescence Resonance Energy Transfer; Hydrolysis; Insecta; Kinetics; Models, Chemical; Models, Molecular; Mutagenesis, Site-Directed; Mutation; Myosin Type II; Myosins; Nucleotides; Protein Conformation; Protein Structure, Tertiary; Temperature; Time Factors; Tryptophan | 2005 |
Nucleotides regulate the conformational state of the small terminase subunit from bacteriophage lambda: implications for the assembly of a viral genome-packaging motor.
Topics: Acrylamide; Adenosine Diphosphate; Adenosine Triphosphate; Azides; Bacteriophage lambda; Binding, Competitive; Dimerization; DNA-Binding Proteins; DNA, Viral; Endodeoxyribonucleases; Genome, Viral; Molecular Motor Proteins; Photoaffinity Labels; Protein Binding; Protein Conformation; Protein Subunits; Sequence Deletion; Spectrometry, Fluorescence; Tryptophan; Viral Proteins; Virus Assembly | 2005 |
Effect of structural transition of the host assembly on dynamics of an ion channel peptide: a fluorescence approach.
Topics: Acrylamide; Bacillus; Circular Dichroism; Dose-Response Relationship, Drug; Gramicidin; Ion Channels; Ions; Kinetics; Micelles; Models, Statistical; Protein Conformation; Sodium Dodecyl Sulfate; Solvents; Spectrometry, Fluorescence; Spectrophotometry; Spectrum Analysis, Raman; Time Factors; Tryptophan; Ultraviolet Rays; Water | 2005 |
Conformational dynamics of estrogen receptors alpha and beta as revealed by intrinsic tryptophan fluorescence and circular dichroism.
Topics: Acrylamide; Circular Dichroism; Estradiol; Estrogen Receptor alpha; Estrogen Receptor beta; Female; Humans; Male; Microscopy, Atomic Force; Particle Size; Protein Conformation; Protein Denaturation; Protein Folding; Protein Isoforms; Recombinant Proteins; Tryptophan | 2005 |
Membrane-bound peptides mimicking transmembrane Vph1p helix 7 of yeast V-ATPase: a spectroscopic and polarity mismatch study.
Topics: Acrylamide; Amino Acid Sequence; Amino Acids; Arginine; Cell Membrane; Circular Dichroism; Electron Spin Resonance Spectroscopy; Histidine; Lipid Bilayers; Lipids; Models, Statistical; Molecular Sequence Data; Mutation; Peptides; Protein Binding; Protein Conformation; Protein Structure, Secondary; Protein Structure, Tertiary; Saccharomyces cerevisiae; Spectrometry, Fluorescence; Spectrophotometry; Tryptophan; Vacuolar Proton-Translocating ATPases | 2005 |
Anomalous "unquenching" of the fluorescence decay times of beta-lactoglobulin induced by the known quencher acrylamide.
Topics: Acrylamide; Fluorescence; Hydrogen-Ion Concentration; Lactoglobulins; Models, Molecular; Molecular Structure; Time Factors; Tryptophan | 2006 |
Low versus high molecular weight poly(ethylene glycol)-induced states of stem bromelain at low pH: stabilization of molten globule and unfolded states.
Topics: Acrylamide; Anilino Naphthalenesulfonates; Biopolymers; Bromelains; Circular Dichroism; Enzyme Stability; Ethylene Glycol; Guanidine; Hydrogen-Ion Concentration; Molecular Conformation; Molecular Weight; Polyethylene Glycols; Protein Conformation; Protein Denaturation; Protein Folding; Protein Structure, Secondary; Protein Structure, Tertiary; Spectrometry, Fluorescence; Spectrophotometry; Tryptophan | 2006 |
Steady-state fluorescence quenching applications for studying protein structure and dynamics.
Topics: Acrylamide; Algorithms; Cesium; Chlorides; Energy Transfer; Fluorescence; Fluorescence Resonance Energy Transfer; Protein Conformation; Proteins; Temperature; Tryptophan | 2006 |
Characterization of oligomers during alpha-synuclein aggregation using intrinsic tryptophan fluorescence.
Topics: Acrylamide; alpha-Synuclein; Amino Acid Substitution; Amyloid; Anilino Naphthalenesulfonates; Fluorescence; Fluorescence Polarization; Fluorescence Resonance Energy Transfer; Protein Structure, Secondary; Solvents; Time Factors; Tryptophan; Tyrosine | 2006 |
Tryptophan rotamers as evidenced by X-ray, fluorescence lifetimes, and molecular dynamics modeling.
Topics: Acrylamide; Bacillus; Biophysics; Kinetics; Models, Molecular; Models, Statistical; Mutation; Solvents; Spectrometry, Fluorescence; Thermodynamics; Time Factors; Tryptophan; X-Rays | 2006 |
A carboxyl-terminal hydrophobic interface is critical to sodium channel function. Relevance to inherited disorders.
Topics: Acrylamide; Amino Acid Sequence; Amino Acid Substitution; Arrhythmias, Cardiac; Cell Line; Computational Biology; EF Hand Motifs; Humans; Hydrophobic and Hydrophilic Interactions; Ion Channel Gating; Molecular Sequence Data; Muscle Proteins; NAV1.5 Voltage-Gated Sodium Channel; Peptide Fragments; Protein Structure, Secondary; Protein Subunits; Sodium Channels; Spectrometry, Fluorescence; Thermodynamics; Tryptophan | 2006 |
Conformational and functional characterization of trapped complexes of the P-glycoprotein multidrug transporter.
Topics: Acrylamide; Adenosine Diphosphate; Adenosine Triphosphate; Animals; ATP Binding Cassette Transporter, Subfamily B, Member 1; Cesium; CHO Cells; Cricetinae; Cricetulus; Fluorescence; Iodine; Kinetics; Multidrug Resistance-Associated Proteins; Protein Conformation; Trinitrobenzenes; Tryptophan | 2006 |
Effects of guanidine hydrochloride on the conformation and enzyme activity of streptomycin adenylyltransferase monitored by circular dichroism and fluorescence spectroscopy.
Topics: Acrylamide; Anilino Naphthalenesulfonates; Circular Dichroism; Guanidine; Mycobacterium tuberculosis; Nucleotidyltransferases; Potassium Iodide; Protein Binding; Protein Conformation; Protein Structure, Secondary; Spectrometry, Fluorescence; Tryptophan | 2006 |
Modulation of gramicidin channel conformation and organization by hydrophobic mismatch in saturated phosphatidylcholine bilayers.
Topics: Acrylamide; Centrifugation, Density Gradient; Circular Dichroism; Gramicidin; Hydrophobic and Hydrophilic Interactions; Ion Channels; Lipid Bilayers; Phosphatidylcholines; Protein Structure, Quaternary; Spectrometry, Fluorescence; Time Factors; Tryptophan | 2007 |
The role of cofactor binding in tryptophan accessibility and conformational stability of His-tagged D-amino acid oxidase from Trigonopsis variabilis.
Topics: Acrylamide; Amino Acid Sequence; D-Amino-Acid Oxidase; Models, Molecular; Molecular Sequence Data; Protein Binding; Protein Conformation; Protein Denaturation; Spectrophotometry, Ultraviolet; Tryptophan; Yeasts | 2007 |
Effect of structural transition of the host assembly on dynamics of a membrane-bound tryptophan analogue.
Topics: Acrylamide; Anisotropy; Cell Membrane; Membrane Proteins; Micelles; Models, Chemical; Sodium Dodecyl Sulfate; Spectrometry, Fluorescence; Tryptophan | 2007 |
Fluorescence quenching and time-resolved fluorescence studies of alpha-mannosidase from Aspergillus fischeri (NCIM 508).
Topics: Acrylamide; alpha-Mannosidase; Aspergillus; Bromosuccinimide; Cesium; Chlorides; Potassium Iodide; Protein Conformation; Protein Denaturation; Spectrometry, Fluorescence; Succinimides; Tryptophan | 2007 |
Tryptophan fluorescence reveals the presence of long-range interactions in the denatured state of ribonuclease Sa.
Topics: Acrylamide; Acrylamides; Amino Acid Sequence; Disulfides; Fluorescence; Iodides; Molecular Conformation; Molecular Sequence Data; Peptides; Proteins; Ribonucleases; Spectrometry, Fluorescence; Time Factors; Tryptophan; Urea | 2008 |
Membrane-bound peptides from V-ATPase subunit a do not interact with an indole-type inhibitor.
Topics: Acrylamide; Amino Acid Sequence; Amino Acids; Arginine; Fluorescence Resonance Energy Transfer; Indoles; Lipid Bilayers; Lipids; Membrane Proteins; Molecular Sequence Data; Peptides; Phosphatidylcholines; Phosphatidylglycerols; Piperidines; Protein Binding; Protein Conformation; Protein Structure, Secondary; Protein Structure, Tertiary; Saccharomyces cerevisiae; Spectrometry, Fluorescence; Spectrophotometry; Tryptophan; Vacuolar Proton-Translocating ATPases | 2008 |
Binding of LL-37 to model biomembranes: insight into target vs host cell recognition.
Topics: Acrylamide; Amyloid; Antimicrobial Cationic Peptides; Cathelicidins; Circular Dichroism; Fluorescence Resonance Energy Transfer; Humans; Kinetics; Lipid Bilayers; Liposomes; Microbial Sensitivity Tests; Phosphatidylcholines; Protein Binding; Protein Structure, Secondary; Spectrometry, Fluorescence; Time Factors; Tryptophan | 2008 |
Molecularly imprinted polymer prepared with bonded beta-cyclodextrin and acrylamide on functionalized silica gel for selective recognition of tryptophan in aqueous media.
Topics: Acrylamide; Adsorption; beta-Cyclodextrins; Chromatography, High Pressure Liquid; Molecular Imprinting; Silica Gel; Silicon Dioxide; Stereoisomerism; Tryptophan | 2008 |
The secondary structure of calcineurin regulatory region and conformational change induced by calcium/calmodulin binding.
Topics: Acrylamide; Calcineurin; Calcium; Calmodulin; Catalytic Domain; Humans; Models, Biological; Molecular Conformation; Peptides; Protein Binding; Protein Conformation; Protein Structure, Secondary; Protein Structure, Tertiary; Spectroscopy, Fourier Transform Infrared; Tryptophan | 2008 |
Analysis of a membrane interacting region of herpes simplex virus type 1 glycoprotein H.
Topics: Acrylamide; Amino Acid Sequence; Amino Acids; Circular Dichroism; Gene Expression Regulation, Viral; Kinetics; Magnetic Resonance Spectroscopy; Models, Biological; Molecular Sequence Data; Mutation; Peptide Hydrolases; Protein Structure, Secondary; Tryptophan; Viral Envelope Proteins | 2008 |
Benzodiazepine-mediated structural changes in the multidrug transporter P-glycoprotein: an intrinsic fluorescence quenching analysis.
Topics: Acrylamide; Adenosine Triphosphate; Animals; Anti-Anxiety Agents; ATP Binding Cassette Transporter, Subfamily B; Benzodiazepines; Flurazepam; Iodides; Mice; Micelles; Pichia; Protein Conformation; Spectrometry, Fluorescence; Tryptophan | 2008 |
No effect of covalently linked poly(ethylene glycol) chains on protein internal dynamics.
Topics: Acrylamide; Azurin; Enzyme Stability; Luminescent Measurements; Oxygen; Polyethylene Glycols; Protein Conformation; Protein Folding; Proteins; Ribonuclease T1; Spectrometry, Fluorescence; Tryptophan | 2009 |
Equilibrium unfolding of the retinoid X receptor ligand binding domain and characterization of an unfolding intermediate.
Topics: Acrylamide; Alitretinoin; Circular Dichroism; Dose-Response Relationship, Drug; Fluorescein; Fluorescent Dyes; Humans; Ligands; Nitrates; Protein Denaturation; Protein Folding; Protein Multimerization; Protein Structure, Quaternary; Protein Structure, Tertiary; Retinoid X Receptors; Thermodynamics; Tretinoin; Tryptophan; Ultracentrifugation | 2009 |
Trp-107 and trp-253 account for the increased steady state fluorescence that accompanies the conformational change in human pancreatic triglyceride lipase induced by tetrahydrolipstatin and bile salt.
Topics: Acrylamide; Colipases; Humans; Kinetics; Lactones; Lipase; Mutant Proteins; Orlistat; Protein Conformation; Spectrometry, Fluorescence; Structure-Activity Relationship; Taurodeoxycholic Acid; Time Factors; Tryptophan | 2009 |
Light-induced aggregation of type I soluble tumor necrosis factor receptor.
Topics: Acrylamide; Excipients; Oxidation-Reduction; Protein Conformation; Protein Stability; Receptors, Tumor Necrosis Factor, Type I; Sodium Azide; Sulfhydryl Compounds; Tryptophan; Ultraviolet Rays | 2009 |
Acrylamide quenching of Trp phosphorescence in liver alcohol dehydrogenase: evidence of gated quencher penetration.
Topics: Acrylamide; Alcohol Dehydrogenase; Animals; Crystallography, X-Ray; Diffusion; Dose-Response Relationship, Drug; Horses; Liver; Luminescent Measurements; Models, Chemical; Solutions; Temperature; Tryptophan | 2009 |
Biophysical studies of the membrane location of the voltage-gated sensors in the HsapBK and KvAP K(+) channels.
Topics: Acrylamide; Biophysical Phenomena; Circular Dichroism; Desulfurococcaceae; Dimyristoylphosphatidylcholine; Humans; Ion Channel Gating; Large-Conductance Calcium-Activated Potassium Channels; Lipid Bilayers; Micelles; Models, Molecular; Nuclear Magnetic Resonance, Biomolecular; Phospholipid Ethers; Potassium Channels; Protein Structure, Tertiary; Spectrometry, Fluorescence; Tryptophan | 2009 |
Tryptophan exposure and accessibility in the chitooligosaccharide-specific phloem exudate lectin from pumpkin (Cucurbita maxima). A fluorescence study.
Topics: Acrylamide; Cucurbita; Fluorescence; Ligands; Oligosaccharides; Plant Lectins; Protein Denaturation; Spectrometry, Fluorescence; Tryptophan | 2009 |
Different modes of antibiotic action of homodimeric and monomeric bactenecin, a cathelicidin-derived antibacterial peptide.
Topics: Acrylamide; Anti-Infective Agents; Cell Membrane; Dimerization; Escherichia coli; Fluorescence; Kinetics; Lipid Bilayers; Liposomes; Peptide Fragments; Peptides, Cyclic; Sodium Chloride; Staphylococcus aureus; Tryptophan | 2009 |
Rhodotorula aurantiaca penicillin V acylase: active site characterization and fluorometric studies.
Topics: Acrylamide; Bromosuccinimide; Catalytic Domain; Circular Dichroism; Fluorescence; Kinetics; Penicillin Amidase; Phenylmethylsulfonyl Fluoride; Rhodotorula; Spectrometry, Fluorescence; Tryptophan | 2009 |
Enantioseparation and amperometric detection of chiral compounds by in situ molecular imprinting on the microchannel wall.
Topics: Acrylamide; Chemical Fractionation; Cross-Linking Reagents; Electrochemistry; Methacrylates; Microfluidic Analytical Techniques; Molecular Imprinting; Stereoisomerism; Tryptophan | 2009 |
Fluorescence quenching of buried Trp residues by acrylamide does not require penetration of the protein fold.
Topics: Acrylamide; Animals; Fish Proteins; Fishes; Fluorescence; Parvalbumins; Protein Conformation; Protein Folding; Proteins; Ribonuclease T1; Tryptophan | 2010 |
Interaction with membrane mimics of transmembrane fragments 16 and 17 from the human multidrug resistance ABC transporter 1 (hMRP1/ABCC1) and two of their tryptophan variants.
Topics: Acrylamide; Amino Acid Sequence; Anisotropy; Buffers; Cell Membrane; Circular Dichroism; Culture Media; Glucosides; Halogenation; Humans; Micelles; Molecular Mimicry; Molecular Sequence Data; Multidrug Resistance-Associated Proteins; Mutant Proteins; Peptide Fragments; Phosphorylcholine; Protein Binding; Protein Structure, Secondary; Spectrometry, Fluorescence; Time Factors; Titrimetry; Tryptophan | 2010 |
The acrylamide (S)-2 as a positive and negative modulator of Kv7 channels expressed in Xenopus laevis oocytes.
Topics: Acrylamide; Acrylamides; Animals; Binding Sites; Carbamates; Humans; Ion Channel Gating; KCNQ Potassium Channels; Kinetics; Membrane Potentials; Mutant Proteins; Oocytes; Oxazines; Phenylenediamines; Tryptophan; Xenopus laevis | 2009 |
Identification and characterization of molten globule-like state of hen egg-white lysozyme in presence of salts under alkaline conditions.
Topics: Acrylamide; Animals; Chickens; Circular Dichroism; Electrophoresis, Polyacrylamide Gel; Hydrogen-Ion Concentration; Muramidase; Potassium Chloride; Protein Conformation; Protein Denaturation; Protein Folding; Salts; Spectrometry, Fluorescence; Tryptophan | 2010 |
The presence of a single N-terminal histidine residue enhances the fusogenic properties of a Membranotropic peptide derived from herpes simplex virus type 1 glycoprotein H.
Topics: Acrylamide; Algorithms; Herpesvirus 1, Human; Histidine; Kinetics; Lipids; Liposomes; Magnetic Resonance Spectroscopy; Peptides; Protein Binding; Protein Structure, Tertiary; Recombinant Fusion Proteins; Surface Plasmon Resonance; Tryptophan; Viral Envelope Proteins | 2010 |
Exploring tryptophan dynamics in acid-induced molten globule state of bovine alpha-lactalbumin: a wavelength-selective fluorescence approach.
Topics: Acids; Acrylamide; Animals; Cattle; Ethylene Chlorohydrin; Fluorescence; Fluorescence Polarization; Hydrogen-Ion Concentration; Lactalbumin; Protein Denaturation; Protein Folding; Time Factors; Tryptophan | 2010 |
Examining the influence of ultraviolet C irradiation on recombinant human γD-crystallin.
Topics: Acrylamide; Chemical Precipitation; Circular Dichroism; Disulfides; Electrophoresis, Polyacrylamide Gel; gamma-Crystallins; Guanidine; Humans; Molecular Weight; Nephelometry and Turbidimetry; Polymerization; Protein Structure, Quaternary; Protein Structure, Secondary; Protein Structure, Tertiary; Recombinant Proteins; Solubility; Spectrometry, Fluorescence; Tryptophan; Ultraviolet Rays | 2010 |
Amplitude spectrum of structural fluctuations in proteins from the internal diffusion of solutes of increasing molecular size: a Trp phosphorescence quenching study.
Topics: Acrylamide; Alcohol Dehydrogenase; Alkaline Phosphatase; Azurin; Diffusion; Glyceraldehyde-3-Phosphate Dehydrogenases; Kinetics; Luminescent Measurements; Models, Molecular; Protein Conformation; Proteins; Solutions; Temperature; Tryptophan | 2011 |
Fluorescence spectroscopy as a probe of the effect of phosphorylation at serine 40 of tyrosine hydroxylase on the conformation of its regulatory domain.
Topics: Acrylamide; Fluorescence Polarization; Phosphorylation; Phosphoserine; Protein Structure, Tertiary; Spectrometry, Fluorescence; Tryptophan; Tyrosine 3-Monooxygenase | 2011 |
Conformational changes of FtsZ reported by tryptophan mutants.
Topics: Acrylamide; Bacterial Proteins; Cytoskeletal Proteins; Microscopy, Electron; Models, Molecular; Mutation; Protein Conformation; Spectrometry, Fluorescence; Tryptophan | 2011 |
Mutational and structural studies of the PixD BLUF output signal that affects light-regulated interactions with PixE.
Topics: Acrylamide; Amino Acid Sequence; Bacterial Proteins; Chromatography, Gel; Crystallography, X-Ray; Darkness; Flavin-Adenine Dinucleotide; Fluorescence; Light; Light Signal Transduction; Magnetic Resonance Spectroscopy; Models, Molecular; Molecular Sequence Data; Mutant Proteins; Mutation; Peptides; Photoreceptors, Microbial; Protein Binding; Protein Structure, Tertiary; Sequence Alignment; Solvents; Spectrophotometry, Ultraviolet; Synechocystis; Tryptophan | 2011 |
Trifluoroethanol stabilizes the molten globule state and induces non-amyloidic turbidity in stem bromelain near its isoelectric point.
Topics: Absorption; Acrylamide; Amyloid; Anilino Naphthalenesulfonates; Bromelains; Circular Dichroism; Enzyme Stability; Guanidine; Isoelectric Point; Nephelometry and Turbidimetry; Protein Binding; Protein Conformation; Protein Denaturation; Spectrometry, Fluorescence; Temperature; Trifluoroethanol; Tryptophan | 2011 |
Biophysical characterization and membrane interaction of the two fusion loops of glycoprotein B from herpes simplex type I virus.
Topics: Acrylamide; Amino Acid Motifs; Biophysics; Crystallography, X-Ray; Herpesvirus 1, Human; Lipids; Peptides; Protein Structure, Secondary; Spectrometry, Fluorescence; Tryptophan; Tyrosine; Viral Envelope Proteins | 2012 |
Differing structural characteristics of molten globule intermediate of peanut lectin in urea and guanidine-HCl.
Topics: Acrylamide; Anilino Naphthalenesulfonates; Bromosuccinimide; Guanidine; Peanut Agglutinin; Protein Binding; Protein Denaturation; Protein Structure, Tertiary; Protein Unfolding; Temperature; Tryptophan; Urea | 2012 |
Stabilization of ferrochelatase via lysine residues on the carboxyl terminal extension.
Topics: Acrylamide; Animals; Chironomidae; Enzyme Stability; Ferrochelatase; Guanidine; Insect Proteins; Lysine; Mutation; Protein Conformation; Protein Denaturation; Spectrometry, Fluorescence; Structural Homology, Protein; Tryptophan | 2013 |
Interaction with lipid II induces conformational changes in bovicin HC5 structure.
Topics: Acrylamide; Amino Acid Sequence; Bacteriocins; Circular Dichroism; Hydrogen-Ion Concentration; Membranes, Artificial; Molecular Sequence Data; Protein Binding; Protein Conformation; Protein Stability; Spectrometry, Fluorescence; Streptococcus bovis; Tryptophan; Uridine Diphosphate N-Acetylmuramic Acid | 2012 |
Detection and analysis of protofibrils and fibrils of hemoglobin: implications for the pathogenesis and cure of heme loss related maladies.
Topics: Absorption; Acrylamide; Anilino Naphthalenesulfonates; Animals; Cattle; Dose-Response Relationship, Drug; Hematologic Diseases; Heme; Hemoglobins; Protein Multimerization; Spectrometry, Fluorescence; Trifluoroethanol; Tryptophan | 2013 |
Monomeric banana lectin at acidic pH overrules conformational stability of its native dimeric form.
Topics: Acids; Acrylamide; Anilino Naphthalenesulfonates; Chromatography, Gel; Circular Dichroism; Hydrodynamics; Hydrogen-Ion Concentration; Kinetics; Lectins; Light; Musa; Protein Binding; Protein Denaturation; Protein Multimerization; Protein Stability; Protein Structure, Secondary; Protein Structure, Tertiary; Protein Unfolding; Scattering, Radiation; Software; Spectrometry, Fluorescence; Temperature; Tryptophan; Urea | 2013 |
Membrane bound α-synuclein is fully embedded in the lipid bilayer while segments with higher flexibility remain.
Topics: Acrylamide; alpha-Synuclein; Amino Acid Sequence; Cell Membrane; Humans; Lipid Bilayers; Lipids; Micelles; Molecular Sequence Data; Parkinson Disease; Phosphatidylcholines; Phosphatidylserines; Protein Binding; Protein Structure, Tertiary; Tryptophan | 2013 |
Understanding the relevance of local conformational stability and dynamics to the aggregation propensity of an IgG1 and IgG2 monoclonal antibodies.
Topics: Acrylamide; Antibodies, Monoclonal; Circular Dichroism; Fluorescence; Immunoglobulin G; Kinetics; Multiprotein Complexes; Protein Conformation; Protein Folding; Protein Multimerization; Protein Stability; Temperature; Tryptophan | 2013 |
A comparative study of unfolding of Erythrina cristagalli Lectin in chemical denaturant and Fluoroalcohols.
Topics: Acrylamide; Anilino Naphthalenesulfonates; Bromosuccinimide; Erythrina; Guanidine; Hydrogen-Ion Concentration; Oxidation-Reduction; Plant Lectins; Propanols; Protein Binding; Protein Denaturation; Protein Structure, Tertiary; Spectrometry, Fluorescence; Trifluoroethanol; Tryptophan | 2014 |
Thermally stable harpin, HrpZPss is sensitive to chemical denaturants: probing tryptophan environment, chemical and thermal unfolding by fluorescence spectroscopy.
Topics: Acrylamide; Bacterial Outer Membrane Proteins; Calorimetry, Differential Scanning; Guanidine; Kinetics; Protein Denaturation; Protein Stability; Protein Unfolding; Spectrometry, Fluorescence; Tryptophan; Urea | 2013 |
A molten globule intermediate of the von Willebrand factor A1 domain firmly tethers platelets under shear flow.
Topics: Acrylamide; Blood Platelets; Chromatography, Gel; Disulfides; Guanidine; Humans; Oxidation-Reduction; Platelet Adhesiveness; Protein Denaturation; Protein Structure, Tertiary; Rheology; Shear Strength; Spectrometry, Fluorescence; Temperature; Tryptophan; von Willebrand Factor | 2014 |
Picosecond-resolved fluorescent probes at functionally distinct tryptophans within a thermophilic alcohol dehydrogenase: relationship of temperature-dependent changes in fluorescence to catalysis.
Topics: Acrylamide; Alcohol Dehydrogenase; Bacterial Proteins; Biocatalysis; Catalytic Domain; Enzyme Stability; Fluorescence; Fluorescent Dyes; Geobacillus stearothermophilus; Kinetics; Mutagenesis, Site-Directed; Phenylalanine; Spectrometry, Fluorescence; Temperature; Thermodynamics; Tryptophan; Zinc | 2014 |
Design and characterization of short antimicrobial peptides using leucine zipper templates with selectivity towards microorganisms.
Topics: 3T3 Cells; Acrylamide; Animals; Anti-Bacterial Agents; Antimicrobial Cationic Peptides; Drug Design; Erythrocytes; Humans; Leucine; Leucine Zippers; Lipids; Mice; Tryptophan | 2014 |
Specific and ultrasensitive ciprofloxacin detection by responsive photonic crystal sensor.
Topics: Acrylamide; Ciprofloxacin; Hydrogen-Ion Concentration; Optics and Photonics; Osmolar Concentration; Tryptophan; Water Pollutants, Chemical; Zinc | 2014 |
Insights into the membrane interaction mechanism and antibacterial properties of chensinin-1b.
Topics: Acrylamide; Amino Acid Sequence; Animals; Anti-Bacterial Agents; Antimicrobial Cationic Peptides; Cell Membrane; Cell Membrane Permeability; Circular Dichroism; Escherichia coli; Female; Fluoresceins; Hemolysis; Humans; Kinetics; Lipopolysaccharides; Liposomes; Mice; Microbial Sensitivity Tests; Molecular Sequence Data; Peptides; Potassium Iodide; Protein Binding; Protein Structure, Secondary; Spectrometry, Fluorescence; Thermodynamics; Time Factors; Tryptophan; Wound Healing | 2015 |
pH-induced structural change of a multi-tryptophan protein MPT63 with immunoglobulin-like fold: identification of perturbed tryptophan residue/residues.
Topics: Acrylamide; Amino Acid Motifs; Anilino Naphthalenesulfonates; Bacterial Proteins; Benzothiazoles; Cloning, Molecular; Escherichia coli; Fluorescent Dyes; Gene Expression; Hydrogen Bonding; Hydrogen-Ion Concentration; Immunoglobulins; Molecular Sequence Data; Mycobacterium tuberculosis; Protein Folding; Protein Structure, Secondary; Protein Structure, Tertiary; Recombinant Proteins; Spectrometry, Fluorescence; Static Electricity; Thermodynamics; Thiazoles; Tryptophan; Tyrosine | 2015 |
Spectroscopic Characterization of Structural Changes in Membrane Scaffold Proteins Entrapped within Mesoporous Silica Gel Monoliths.
Topics: Acrylamide; Amino Acid Sequence; Anisotropy; Circular Dichroism; Lipoproteins; Membrane Proteins; Molecular Sequence Data; Nanoparticles; Porosity; Silica Gel; Solutions; Spectrometry, Fluorescence; Tryptophan | 2015 |
Insights into Kinetics of Agitation-Induced Aggregation of Hen Lysozyme under Heat and Acidic Conditions from Various Spectroscopic Methods.
Topics: Acrylamide; Amyloid; Animals; Benzothiazoles; Chickens; Chromatography, High Pressure Liquid; Hot Temperature; Hydrogen-Ion Concentration; Kinetics; Light; Microscopy, Atomic Force; Muramidase; Protein Denaturation; Protein Folding; Protein Structure, Secondary; Scattering, Radiation; Spectrophotometry; Spectroscopy, Fourier Transform Infrared; Thiazoles; Time Factors; Tryptophan | 2015 |
Carbonyl-based blue autofluorescence of proteins and amino acids.
Topics: Acrylamide; Amino Acids; Fluorescence; Lactoglobulins; Optical Imaging; Proline; Proteins; Solutions; Spectrometry, Fluorescence; Tryptophan | 2017 |
Triplet state spectroscopy reveals involvement of the buried tryptophan residue 310 in Glyceraldehyde-3-phosphate dehydrogenase (GAPD) in the interaction with acrylamide.
Topics: Acrylamide; Animals; Glyceraldehyde-3-Phosphate Dehydrogenases; Molecular Docking Simulation; Rabbits; Spectrometry, Fluorescence; Tryptophan | 2024 |