acid-phosphatase and zinc-iodide

acid-phosphatase has been researched along with zinc-iodide* in 2 studies

Other Studies

2 other study(ies) available for acid-phosphatase and zinc-iodide

Article	Year
ZIO impregnation and cytochemical localization of thiamine pyrophosphatase and acid phosphatase activities in small granule-containing (SGC) cells of rat superior cervical ganglia. Histology and histopathology, 1994, Volume: 9, Issue:4 Cytochemical relationship between Golgi complex and dense-cored granules (DCGs) of small granule-containing (SGC) cells in rat superior cervical ganglia was examined in electron microscopy by zinc-iodide-osmium tetroxide (ZIO) method and by enzyme cytochemistry for thiamine pyrophosphatase (TPPase) and acid phosphatase (ACPase). After ZIO impregnation, all the saccules of Golgi apparatus and some of tubular rough endoplasmic reticulum (rER) were stained. DCGs in periphery of SGC cells were not stained, but varying degrees of dense deposits occurred in the DCGs in vicinity of Golgi trans-saccules. Both TPPase and ACPase activities were localized in one or two stacked layers of saccules on the trans side of the Golgi complex. No reaction products were demonstrated in the DCGs. From these results, we suggest that the DCGs of SGC cells in rat superior cervical ganglia are derived from the Golgi complex, and that lysosomal cleavage of protein contents in the DCGs may occur in the trans Golgi saccules. Topics: Acid Phosphatase; Animals; Cytoplasmic Granules; Female; Golgi Apparatus; Histocytochemistry; Iodides; Male; Microscopy, Electron; Osmium Tetroxide; Rats; Staining and Labeling; Superior Cervical Ganglion; Thiamine Pyrophosphatase; Zinc Compounds	1994
Ultracytochemical evidence for the presence of GERL in pinealocytes of the Mongolian gerbil (Meriones unguiculatus). Cell and tissue research, 1986, Volume: 246, Issue:3 Ultracytochemical reactions for the demonstration of acid phosphatase, glucose-6-phosphatase and thiamine pyrophosphatase, as well as zinc iodide-osmium tetroxide impregnation, revealed the existence of GERL (Golgi apparatus-Endoplasmic Reticulum-Lysosomes) in pinealocytes of the Mongolian gerbil (Meriones unguiculatus). The spatial arrangement of this structure was studied on thick sections using a goniometric stage. Although it was not possible to determine whether GERL in pinealocytes belongs to the Golgi apparatus or to endoplasmic reticulum, it can be concluded that its presence in studied cells signifies that they are considerably more active synthetically than has been believed to date. Topics: Acid Phosphatase; Animals; Endoplasmic Reticulum; Gerbillinae; Glucose-6-Phosphatase; Golgi Apparatus; Histocytochemistry; Iodides; Lysosomes; Male; Osmium Tetroxide; Pineal Gland; Thiamine Pyrophosphatase; Zinc; Zinc Compounds	1986

Article

Year

ZIO impregnation and cytochemical localization of thiamine pyrophosphatase and acid phosphatase activities in small granule-containing (SGC) cells of rat superior cervical ganglia.

Histology and histopathology, 1994, Volume: 9, Issue:4

Cytochemical relationship between Golgi complex and dense-cored granules (DCGs) of small granule-containing (SGC) cells in rat superior cervical ganglia was examined in electron microscopy by zinc-iodide-osmium tetroxide (ZIO) method and by enzyme cytochemistry for thiamine pyrophosphatase (TPPase) and acid phosphatase (ACPase). After ZIO impregnation, all the saccules of Golgi apparatus and some of tubular rough endoplasmic reticulum (rER) were stained. DCGs in periphery of SGC cells were not stained, but varying degrees of dense deposits occurred in the DCGs in vicinity of Golgi trans-saccules. Both TPPase and ACPase activities were localized in one or two stacked layers of saccules on the trans side of the Golgi complex. No reaction products were demonstrated in the DCGs. From these results, we suggest that the DCGs of SGC cells in rat superior cervical ganglia are derived from the Golgi complex, and that lysosomal cleavage of protein contents in the DCGs may occur in the trans Golgi saccules.

Topics: Acid Phosphatase; Animals; Cytoplasmic Granules; Female; Golgi Apparatus; Histocytochemistry; Iodides; Male; Microscopy, Electron; Osmium Tetroxide; Rats; Staining and Labeling; Superior Cervical Ganglion; Thiamine Pyrophosphatase; Zinc Compounds

1994

Ultracytochemical evidence for the presence of GERL in pinealocytes of the Mongolian gerbil (Meriones unguiculatus).

Cell and tissue research, 1986, Volume: 246, Issue:3

Ultracytochemical reactions for the demonstration of acid phosphatase, glucose-6-phosphatase and thiamine pyrophosphatase, as well as zinc iodide-osmium tetroxide impregnation, revealed the existence of GERL (Golgi apparatus-Endoplasmic Reticulum-Lysosomes) in pinealocytes of the Mongolian gerbil (Meriones unguiculatus). The spatial arrangement of this structure was studied on thick sections using a goniometric stage. Although it was not possible to determine whether GERL in pinealocytes belongs to the Golgi apparatus or to endoplasmic reticulum, it can be concluded that its presence in studied cells signifies that they are considerably more active synthetically than has been believed to date.

Topics: Acid Phosphatase; Animals; Endoplasmic Reticulum; Gerbillinae; Glucose-6-Phosphatase; Golgi Apparatus; Histocytochemistry; Iodides; Lysosomes; Male; Osmium Tetroxide; Pineal Gland; Thiamine Pyrophosphatase; Zinc; Zinc Compounds

1986