acid-phosphatase has been researched along with osmium-zinc-iodide* in 2 studies
2 other study(ies) available for acid-phosphatase and osmium-zinc-iodide
| Article | Year |
|---|---|
A cytochemical study of dense granules in the rat junctional epithelium.
To clarify the details of intracytoplasmic granules in the junctional epithelium (JE) of the rat gingiva, phosphotungstic acid (PTA) staining, acid phosphatase cytochemistry and zinc iodide-osmium (ZIO) staining were carried out. Condensing granules containing homogeneous electron-dense materials, measuring about 260 nm in diameter, have been observed at the peripheral cytoplasm of the JE. These granules reveal PTA-positive, ZIO-positive and acid phosphatase-positive staining. These results indicate that the granules contain glycoprotein, phospholipid, lipoprotein and lysosomal enzymes. From their composition and morphology, it is suggested that these granules in the JE may contribute secretory function and secrete glycoproteins or glycolipids. Although it is unclear whether the dense granules correspond to membrane-coating granules by our staining methods, it is surmised that, if the dense granules can engage in establishment of the barrier function, then this role is impeded by the large intercellular space in the JE. Topics: Acid Phosphatase; Animals; Cytoplasmic Granules; Epithelium; Gingiva; Histocytochemistry; Male; Microscopy, Electron; Osmium; Osmium Tetroxide; Phosphotungstic Acid; Rats; Rats, Inbred Strains; Staining and Labeling; Zinc; Zinc Compounds | 1989 |
Fine structural and cytochemical studies on the hamster subcommissural organ.
The subcommissural organ (SCO) of the golden hamster (Mesocricetus auratus) was studied by conventional electron microscopy, freeze-fracture technique, zinc-iodide-osmium (ZIO) and acid phosphatase cytochemical reactions. The ultrastructure of hamster SCO cells shows a few flattened cisternae of rough endoplasmic reticulum (ER) without dilated ones in the cytoplasm. The Golgi apparatus is very well developed. Freeze-fracture studies also indicate only short profiles of flattened ER in the cytoplasm endorsing the absence of dilated ER cisternae. After the treatment with ZIO mixture, reaction products were observed over flattened cisternae of the ER and the nuclear envelope. The Golgi apparatus was also reactive toward the ZIO mixture. Acid phosphatase activities are localized in the inner one or two saccules of the Golgi apparatus and dense bodies. From these results we suggest that (1) hamster SCO cells do not accumulate secretory material in the cytoplasm in the form of discrete secretory granules or dilated cisternae of ER, and (2) hamster SCO cells may possess extremely high secretory activity or may not be actively involved in secretory function at all as in rats or other rodents. Topics: Acid Phosphatase; Animals; Cricetinae; Cytoplasm; Endoplasmic Reticulum; Female; Freeze Fracturing; Golgi Apparatus; Histocytochemistry; Intercellular Junctions; Male; Mesocricetus; Microscopy, Electron; Mitochondria; Neurosecretory Systems; Osmium; Osmium Tetroxide; Staining and Labeling; Subcommissural Organ; Zinc; Zinc Compounds | 1987 |