acid-phosphatase and mezerein

Cells of U937, a human monocytic leukemia cell line, differentiate into macrophages by treatment with 12-o-tetradecanoylphorbol-13-acetate (TPA), whereas cells treated with 1 alpha, 25-dihydroxyvitamin D3 [1,25-(OH)2D3] continue to grow without undergoing differentiation. When U937 cells were successively treated with TPA and 1,25-(OH)2D3, tartrate-resistant acid phosphatase-positive multinucleated cells appeared at 5 days after the treatment. These osteoclast-like cells released a soluble form of 45Ca from 45Ca-labeled bone particles. These cells were not formed when the order of treatment with TPA and 1,25-(OH)2D3 was reversed. Use of either dexamethasone or interferon-gamma (IFN-gamma) was effective in inhibiting the formation of these osteoclast-like cells. The expression of c-src, c-fms, and macrophage colony stimulating factor (M-CSF) was induced by TPA treatment; however, TPA-induced M-CSF gene transcription was attenuated by the subsequent addition of 1,25-(OH)2D3. Furthermore, both dexamethasone and IFN-gamma impaired the attenuation of M-CSF expression, suggesting that the transient expression of M-CSF may be important for the formation of osteoclast-like cells.

Topics: Acid Phosphatase; Animals; Calcitonin; Calcitriol; Carcinogens; Cell Differentiation; Cell Nucleus; Dexamethasone; Diterpenes; Drug Synergism; Gene Expression Regulation, Leukemic; Genes, fms; Genes, src; Humans; Interferon-gamma; Isoenzymes; Leukemia, Monocytic, Acute; Macrophage Colony-Stimulating Factor; Mice; Osteoclasts; Phenotype; Tartrate-Resistant Acid Phosphatase; Terpenes; Tetradecanoylphorbol Acetate