acid-phosphatase has been researched along with indoxyl* in 2 studies
2 other study(ies) available for acid-phosphatase and indoxyl
| Article | Year |
|---|---|
Subcutaneous fat necrosis of the newborn: light, ultrastructural and histochemical microscopic studies.
Multiple subcutaneous plaques and nodules appeared on the back and the dorsal proximal area of the extremities of a 9-day-old male infant after a complicated prenatal period necessitating cesarean section. The clinical and histological features were diagnostic of subcutaneous fat necrosis of the newborn. Light microscopy revealed adipocyte necrosis, a lymphohistiocytic infiltrate, and needle-shaped clefts within adipocytes and macrophages. Ultrastructurally, there were aggregations of electron-lucent spaces in the form of spindles and needles arranged in parallel within the altered adipocytes; macrophages surrounded these cells or their fragments and invaded the fat lobules. Enzyme histochemical staining, not previously reported in the literature, showed that acid phosphatase, leucine aminopeptidase, and indoxyl and non-specific esterases were present in the areas of fat necrosis. Topics: Acid Phosphatase; Adipose Tissue; Carboxylesterase; Carboxylic Ester Hydrolases; Histocytochemistry; Humans; Indoles; Infant, Newborn; Infant, Newborn, Diseases; Leucyl Aminopeptidase; Male; Microscopy, Electron; Necrosis; Skin Diseases | 1989 |
Detection of alkaline phosphatase activity after conventional isoelectric focusing by an indoxyl-tetrazolium salt technique.
Alkaline phosphatase was solubilized from human and rat tissues using papain in the presence of TRITON X-100 and subjected to isoelectric focusing (IEF) in polyacrylamide or agarose gels. Up till now, usually 1- and 2-naphthylphosphates have been used as substrates in order to specifically stain molecular forms of this enzyme by the azo-dye technique. In this paper, the use of another histochemical substrate, 5-bromo-4-chloro-3-indoxyl phosphate, in combination with tetrazolium salts [McGadey, J. (1970) Histochemie 23, 180-184] is presented. After hydrolysis, the released indoxyl moieties reduce tetrazolium salts to insoluble formazans at the zones of alkaline phosphatase activity. Zymogrammes showing molecular forms of alkaline phosphatase from 20 rat organs and the application of this staining technique for the detection of alkaline phosphatase activity in non-dialyzed human plasma after IEF are presented. Topics: Acid Phosphatase; Alkaline Phosphatase; Animals; Female; Indoles; Isoelectric Focusing; Isoenzymes; Male; Rats; Rats, Inbred Strains; Solubility; Staining and Labeling; Tetrazolium Salts | 1985 |