acid-phosphatase and gibberellic-acid

Phytic acid as a phosphorus storage vault provides phosphorus for plant development. It is an anti-nutritional factor for humans and some animals. However, its degradation products lower inositol phosphates have positive effects on human health. In this study, the effect of gibberellic acid (GA) on phytic acid degradation under calcium lactate (Ca) existence was investigated.. The results showed that Ca + GA treatment promoted the growth status, hormone metabolism and phytic acid degradation in germinating soybean. At the same time, the availability of phosphorus, the activity of phytic acid degradation-associated enzyme and phosphoinositide-specific phospholipase C (PI-PLC) increased. However, the relative genes expression of phytic acid degradation-associated enzymes did not vary in accordance with their enzymes activity.. The results revealed that GA could mediate the transport and function of calcium and a series of physiological and biochemical changes to regulate phytic acid degradation of soybean sprouts. © 2017 Society of Chemical Industry.

Topics: 6-Phytase; Acid Phosphatase; Calcium Compounds; Gene Expression Regulation, Enzymologic; Gene Expression Regulation, Plant; Germination; Gibberellins; Glycine max; Lactates; Phospholipids; Phosphorus; Phytic Acid; Seeds

The present study investigates the bone maturity of suckling rats whose mothers were treated with gibberellic acid (GA(3)). Female Wistar rats were divided into two groups: group I that served as controls and group II that received orally GA(3) (200 ppm) from the 14th day of pregnancy until day 14 after delivery. In the GA(3) group, an increase in body and femur weights as well as in femur length of pups was noted when compared to controls. Lipid peroxidation was demonstrated by high femur malondialdehyde levels, while superoxide dismutase, catalase, glutathione peroxidase activities, glutathione and vitamin C levels in femur decreased. GA(3) caused a decrease in calcium and phosphorus levels in bone. The calcium concentration in plasma increased and the phosphorus concentration decreased, while urinary levels of calcium decreased and those of phosphate increased. Moreover, plasma total tartrate-resistant acid phosphatase and total alkaline phosphatase increased. Bone disorders were confirmed by femur histological changes.

Topics: Acid Phosphatase; Animals; Animals, Suckling; Ascorbic Acid; Body Weight; Calcium; Catalase; Environmental Pollutants; Female; Femur; Gibberellins; Glutathione; Glutathione Peroxidase; Glutathione Reductase; Isoenzymes; Lipid Peroxidation; Male; Malondialdehyde; Oxidative Stress; Phosphorus; Pregnancy; Rats; Rats, Wistar; Superoxide Dismutase; Tartrate-Resistant Acid Phosphatase

Enzymatic and histological change in the testicular cells of rats treated orally and intradermally for 45 days with gibberellic acid (GBA) in independent studies is reported. Assay of hexokinase (HK), acid phosphatase (AcP) and alkaline phosphatase (AkP) in rat testicular tissue homogenate preparations yielded results that suggested changes in these enzyme activities relative to their respective controls. Histological studies showed loss of germ cells, derangement of the germinal cells, and reduction in the size of the seminiferous tubules and dystrophy of Leydig cells. More importantly decreased sperm count in the lumen was observed. A dysregulatory role is thus established for GBA in rat testicular cell function. This compound may serve as an inhibitor of testicular cell function.

Topics: Acid Phosphatase; Alkaline Phosphatase; Animals; Gibberellins; Hexokinase; Male; Rats; Spermatogenesis; Testis; Tissue Extracts

Topics: Acid Phosphatase; Gibberellins; Indoleacetic Acids; Plant Development; Plants; Plants, Toxic; Ricinus communis

Phytohormonal activity (auxins, gibberellins, cytokinins) was tested in the supernatant of a culture of Arthrobacter sp. Crude extract of the phytohormonal fraction was used as substrate for the growth of Lactuca sativa seedlings. Treating with bacterial hormones resulted in an increased plant develop- ment. Furthermore, a sharp increase of the acid phosphatase activity was observed in the roots.

Topics: Acid Phosphatase; Arthrobacter; Gibberellins; Indoleacetic Acids; Isopentenyladenosine; Plant Development; Plant Growth Regulators; Plants