acid-phosphatase and ethyl-acetate

Medicinal plants are considered immunomodulatory as they display various biological activities. There is no report addressing the anti-inflammatory effects of Daphne gnidium. In this study, we investigated the effects of D. gnidium ethyl acetate (EA) leaf extract on mice immune cell function in vitro. Production of pro-inflammatory cytokines (IL-1β and TNF-α), cyclooxygenase-2-derived prostaglandinE2 (PGE2) and iNOS-II-synthesised nitric oxide (NO) were examined. EA extract effect on mitogen-induced lymphocyte proliferation was also investigated. We reported for the first time that D. gnidium EA leaf extract dose-dependently inhibits macrophage proinflammatory function by reducing LPS-induced production of IL-1β, TNF-α, COX-2-derived PGE2 and iNOS-II-synthesised NO. Mitogen-induced lymphocyte proliferation was also dose-dependently inhibited by the extract. Lectin-induced response appears to be more sensitive to the suppressive effects of the extract than LPS-stimulated response. Collectively, these results demonstrate that D. gnidium EA leaf extract acts as an in vitro anti-inflammatory factor by inhibiting mice macrophage and lymphocyte activities.

Topics: Acetates; Acid Phosphatase; Animals; Cell Proliferation; Cyclooxygenase 2; Daphne; Dinoprostone; Down-Regulation; Flavonoids; In Vitro Techniques; Interleukin-1beta; Lipopolysaccharides; Lymphocyte Activation; Lysosomes; Macrophage Activation; Macrophages, Peritoneal; Male; Mice; Mice, Inbred BALB C; Nitric Oxide; Nitric Oxide Synthase Type II; Phenols; Plant Extracts; Plant Leaves; Polyphenols; T-Lymphocytes; Tumor Necrosis Factor-alpha