acid-phosphatase has been researched along with aniline* in 1 studies
1 other study(ies) available for acid-phosphatase and aniline
| Article | Year |
|---|---|
Colorimetric determination of acid phosphatase activity and inhibitor screening based on in situ polymerization of aniline catalyzed by gold nanoparticles.
A colorimetric assay for acid phosphatase (ACP) was constructed that is based on in situ polymerization of aniline catalyzed by gold nanoparticles (AuNPs). Aniline can be polymerized by ammonium persulfate (APS) in acidic condition and form gold-polyaniline core-shell nanoparticles (Au@PANI NPs) in the presence of AuNPs with the assistance of sodium dodecyl sulfate (SDS). AuNPs were also found to accelerate the polymerization process of aniline and thus shorten the reaction time. Upon the introduction of ascorbic acid (AA), the oxidant APS was consumed via the redox reaction. That led to the suppression of the formation of PANI. Consequently, ACP activity can be supervised on the basis of hydrolysis of 2-phospho-L-ascorbic acid trisodium salt (AAP) catalyzed by ACP to release AA. With the increase of ACP activity, the intensity ratio of the absorbance at λ Topics: Acid Phosphatase; Ammonium Sulfate; Aniline Compounds; Catalysis; Colorimetry; Gold; Humans; Limit of Detection; Metal Nanoparticles; Polymerization; Sodium Dodecyl Sulfate | 2021 |