acid-phosphatase and 4-phenylenediamine

To study the effects of paraphenylenediamine (PPD) and linear alkylbenzene sulphonate (LAS) alone and in combination on the skin.. Forty-eight guinea pigs were divided equally into 4 groups and exposed to PPD (4 mg/kg), LAS (12 mg/kg) and PPD (4 mg/kg) plus LAS (12 mg/kg) for 30 days. The biochemical parameters such as acid phosphatase, gtutathione-s-transferase, glutathione peroxidase, glutathione, lipid peroxidation and histamine contents in exposed skin were estimated. The histopathological examination of the exposed skin was also carried out.. The skin enzymes, lipid peroxidation, and histamine increased while glutathione decreased in skin. The simultaneously exposed group showed additive toxic effects. The histopathological examination showed severe hyperkeratosis, thickening of collagen fibres and vacuolisation of epidermal cells in PPD plus LAS exposed skin.. The findings of the present study suggest that simultaneous exposure to PPD and LAS has additive toxic effects.

Topics: Acid Phosphatase; Alkanesulfonic Acids; Animals; Coloring Agents; Drug Synergism; Glucuronidase; Glutathione; Glutathione Peroxidase; Glutathione Transferase; Guinea Pigs; Histamine; Lipid Peroxidation; Male; Phenylenediamines; Skin; Surface-Active Agents

The guinea pigs were dermally exposed to paraphenylenediamine (PPD) and in presence of an oxidising agent hydrogen peroxide for 15 and 30 d to assess their effects on some enzymes, lipid peroxidation and histamine contents in the skin. The activities of acid and alkaline phosphatases, beta-glucuronidase, gamma glutamyl transpeptidase, histidase and tyrosinase were enhanced after application of either PPD or PPD plus hydrogen peroxide. The lipid peroxidation and histamine contents also showed marked elevation following exposure to the chemicals.

Topics: Acid Phosphatase; Alkaline Phosphatase; Animals; Drug Combinations; Glucuronidase; Guinea Pigs; Histamine; Hydrogen Peroxide; Lipid Peroxidation; Lysosomes; Male; Phenylenediamines; Skin