acid-phosphatase has been researched along with (ethylenedinitrilo)-tetramethylenephosphonic-acid* in 2 studies
2 other study(ies) available for acid-phosphatase and (ethylenedinitrilo)-tetramethylenephosphonic-acid
| Article | Year |
|---|---|
Direct modulatory effect of hexasodium N,N,N',N'-ethylenediamine-tetramethylene-phosphonate on bone cell function in vitro.
The phosphonate hexasodium N,N,N',N'-ethylenediamine-tetramethylenephosphonate (EDITEMP.Na6) reduced alkaline phosphatase (Alp) activity and cAMP response to parathyroid hormone (PTH) in primary cultures of foetal rat calvaria cells in a dose-dependent manner, while not affecting culture DNA content. EDITEMP.Na6 also inhibited the mineralization of three-dimensional bone nodules formed in vitro, but not the number of nodules formed. Bone cell culture DNA content was also reduced by EDITEMP.Na6 but at concentrations in excess of those needed to modulate osteoblastic cell function. Withdrawal of EDITEMP.Na6 led to slow but complete recovery of Alp activity. At EDITEMP.Na6 concentrations of 25 microM and higher, recovery of Alp activity appeared to be independent of protein and/or DNA synthesis. Cell culture acid phosphatase (Acp) activity was not affected by EDITEMP.Na6. The results indicate that EDITEMP.Na6 has a direct inhibitory effect on (mature) osteoblastic cell function. In the presence of bone tissue this inhibition also occurred, although not at a relatively low dose level. Topics: Acid Phosphatase; Alkaline Phosphatase; Animals; Cells, Cultured; Cyclic AMP; Depression, Chemical; DNA Replication; Male; Organ Culture Techniques; Organophosphorus Compounds; Osteoblasts; Parathyroid Hormone; Rats; Rats, Inbred Strains; Skull | 1990 |
Effects of intraperitoneal administration of hexasodium N,N,N',N'-ethylenediaminetetramethylenephosphonate on rat bone metabolism.
The influence of hexasodium N,N,N',N'-ethylenediaminetetramethylenephosphonate (EDITEMP.Na6) on biochemical parameters in bone has been studied to help explain recently reported histological and pathological changes in bone induced by this agent. A single, intraperitoneal (ip) injection of EDITEMP.Na6 (350 mumol/kg on Day 0) significantly decreased the activity of serum alkaline phosphatase (Alp) in mature male Wistar rats for up to 10 days following the injection. However, in immature rats the initial EDITEMP.Na6-induced reduction of serum Alp activity preceded a steady increase in serum Alp activity, which was significantly elevated between Days 6 and 13. This increase coincided with a significant increase in bone Alp activity (an indicator of bone formation) on Day 8. Diaphyseal acid phosphatase (Acp) activity (an indicator of bone resorption) was significantly reduced, on Days 4 and 8 but not on Day 1, following treatment with EDITEMP.Na6. Repeated exposure of immature male Wistar rats to EDITEMP.Na6 (70 mumol/kg/day, ip for 7 days) also led to a significant and persistent increase in serum and bone Alp activities and a reduction in diaphyseal Acp activity. The data are in accord with published histomorphological evidence of reduced bone resorption and suggest delayed mineralization of osteoid tissue laid down during the treatment with EDITEMP.Na6. There were only transient and limited changes in bone DNA content and [3H]thymidine incorporation throughout the studies, indicating the absence of marked cytotoxicity or altered bone cell proliferation associated with EDITEMP.Na6-induced changes in phosphatase enzyme activities. Topics: Acid Phosphatase; Alkaline Phosphatase; Animals; Bone and Bones; Bone Resorption; DNA; Male; Organophosphorus Compounds; Rats; Rats, Inbred Strains; Thymidine | 1990 |