D-xylonic-acid and 1-3-4-butanetriol

The capability of Escherichia coli to catabolize D-xylonate is a crucial component for building and optimizing the Dahms pathway. It relies on the inherent dehydratase and keto-acid aldolase activities of E. coli. Although the biochemical characteristics of these enzymes are known, their inherent expression regulation remains unclear. This knowledge is vital for the optimization of D-xylonate assimilation, especially in addressing the problem of D-xylonate accumulation, which hampers both cell growth and target product formation. In this report, molecular biology techniques and synthetic biology tools were combined to build a simple genetic switch controller for D-xylonate. First, quantitative and relative expression analysis of the gene clusters involved in D-xylonate catabolism were performed, revealing two D-xylonate-inducible operons, yagEF and yjhIHG. The 5'-flanking DNA sequence of these operons were then subjected to reporter gene assays which showed P

Topics: Aldehyde-Lyases; Artificial Gene Fusion; Butanols; Escherichia coli; Gene Expression Profiling; Gene Expression Regulation, Bacterial; Genes, Reporter; Hydro-Lyases; Metabolic Engineering; Promoter Regions, Genetic; Xylose