9-diazomethylanthracene has been researched along with tributyl-phosphate* in 1 studies
1 other study(ies) available for 9-diazomethylanthracene and tributyl-phosphate
| Article | Year |
|---|---|
Specific and rapid assay of urinary oxalic acid using high-performance liquid chromatography.
A high-performance liquid chromatographic (HPLC) method was developed to determine the levels of oxalic acid in the urine. This acid was extracted from urine with tri-n-butyl phosphate and converted into the fluorescent derivative by esterification with 9-anthryldiazomethane (ADAM). The reaction mixture containing the oxalic acid derivative can be directly chromatographed on HPLC using octadecylsilane reverse-phase column monitoring with a fluorophotometric detector. A linear relationship was observed in the range from 1 to 100 micrograms/ml of standard oxalic acid dissolved in saline. Disease-free Japanese adults excrete 23.8 +/- 9.0 mg (mean +/- SD) of oxalic acid per day. This method should prove valuable for routine measurements of urinary oxalic acid as it is accurate, simple, and specific. Topics: Anthracenes; Chromatography, High Pressure Liquid; Colorimetry; Fluorescent Dyes; Humans; Japan; Organophosphates; Oxalates; Reference Values | 1983 |