8-oxo-7-8-dihydrodeoxyguanine and dihydrolipoic-acid

To determine effects of lipoic acid, vitamin E, and cysteine before and after oxidant challenge in cats.. 24 sexually intact adult cats.. Cats were allocated into 4 equal groups. For 25 weeks, group A was fed a control dry diet and groups B, C, and D received this diet supplemented with vitamin E (2200 U/kg [dry matter basis {DMB}]) plus cysteine (9.5 g/kg [DMB]), lipoate (150 mg/kg [DMB]), or all 3 antioxidants together, respectively. Weights were measured every 3 days and venous blood obtained every 5 weeks for CBC; serum biochemical analyses; lymphocyte blastogenesis; thiobarbituric acid reactive substances concentration; and concentrations of plasma protein carbonyl, 8-OH d-guanosine, blood glutathione, plasma amino acid, lipoate, and dihydrolipoate. At 15 weeks, all cats received acetaminophen (9 mg/kg, PO, once), clinical effects were observed, and methemoglobin concentrations were measured.. Lymphocyte blastogenesis increased transiently in group C and D cats. After acetaminophen administration, all groups had transient increases in methemoglobin within 4 hours and mild, brief facial edema; group C had decreased glutathione concentration and increased 8-OH d-guanosine concentration versus controls; and protein carbonyl concentration increased least for group B. Plasma lipoate and dihydrolipoate concentrations peaked by week 10 for groups C and D. Conclusions and Clinical Relevance-Lipoate, vitamin E, and cysteine did not have synergistic effects. Lipoate supplementation (150 mg/kg [DMB]) did not act as an antioxidant but appeared to enhance oxidant effects of acetaminophen. Vitamin E plus cysteine had protective effects.

Topics: 8-Hydroxy-2'-Deoxyguanosine; Acetaminophen; Analgesics, Non-Narcotic; Animals; Antioxidants; Biomarkers; Cats; Chromatography, High Pressure Liquid; Cysteine; Dietary Supplements; Drug Therapy, Combination; Female; Glutathione; Guanine; Lymphocyte Activation; Lymphocytes; Male; Methemoglobin; Thioctic Acid; Vitamin E