8-hydroxyguanine and 8-hydroxydeoxyguanosine-5--monophosphate

8-hydroxyguanine has been researched along with 8-hydroxydeoxyguanosine-5--monophosphate* in 2 studies

Reviews

1 review(s) available for 8-hydroxyguanine and 8-hydroxydeoxyguanosine-5--monophosphate

Article	Year
Mutagenic potentials of damaged nucleic acids produced by reactive oxygen/nitrogen species: approaches using synthetic oligonucleotides and nucleotides: survey and summary. Nucleic acids research, 2003, Jan-15, Volume: 31, Issue:2 DNA and DNA precursors (deoxyribonucleotides) suffer damage by reactive oxygen/nitrogen species. They are important mutagens for organisms, due to their endogenous formation. Damaged DNA and nucleotides cause alterations of the genetic information by the mispairing properties of the damaged bases, such as 8-hydroxyguanine (7,8-dihydro-8-oxoguanine) and 2-hydroxyadenine. Here, the author reviews the mutagenic potentials of damaged bases in DNA and of damaged DNA precursors formed by reactive oxygen/nitrogen species, focusing on the results obtained with synthetic oligonucleotides and 2'-deoxyribonucleoside 5'-triphosphates. Topics: 8-Hydroxy-2'-Deoxyguanosine; Animals; Deoxyguanosine; Deoxyuracil Nucleotides; DNA; DNA Damage; DNA Repair; DNA-Directed DNA Polymerase; Guanine; Guanosine Monophosphate; Humans; Mutation; Reactive Nitrogen Species; Reactive Oxygen Species	2003

Article

Year

Mutagenic potentials of damaged nucleic acids produced by reactive oxygen/nitrogen species: approaches using synthetic oligonucleotides and nucleotides: survey and summary.

Nucleic acids research, 2003, Jan-15, Volume: 31, Issue:2

DNA and DNA precursors (deoxyribonucleotides) suffer damage by reactive oxygen/nitrogen species. They are important mutagens for organisms, due to their endogenous formation. Damaged DNA and nucleotides cause alterations of the genetic information by the mispairing properties of the damaged bases, such as 8-hydroxyguanine (7,8-dihydro-8-oxoguanine) and 2-hydroxyadenine. Here, the author reviews the mutagenic potentials of damaged bases in DNA and of damaged DNA precursors formed by reactive oxygen/nitrogen species, focusing on the results obtained with synthetic oligonucleotides and 2'-deoxyribonucleoside 5'-triphosphates.

Topics: 8-Hydroxy-2'-Deoxyguanosine; Animals; Deoxyguanosine; Deoxyuracil Nucleotides; DNA; DNA Damage; DNA Repair; DNA-Directed DNA Polymerase; Guanine; Guanosine Monophosphate; Humans; Mutation; Reactive Nitrogen Species; Reactive Oxygen Species

2003

Other Studies

1 other study(ies) available for 8-hydroxyguanine and 8-hydroxydeoxyguanosine-5--monophosphate

Article	Year
Analysis of 8-hydroxydeoxyguanosine 5'-monophosphate (8-OH-dGMP) as a reliable marker of cellular oxidative DNA damage after gamma-irradiation. Environmental and molecular mutagenesis, 2003, Volume: 41, Issue:5 In order to improve 8-hydroxyguanine (8-OH-Gua) detection in DNA, we digested isolated DNA with nuclease P1 and analyzed for 8-hydroxydeoxyguanosine 5'-monophosphate (8-OH-dGMP) using a high-performance liquid chromatography system equipped with an electrochemical detector (HPLC-ECD). The amount of 8-OH-Gua in the DNA was expressed as the ratio of 8-OH-dGMP to deoxycytidine monophosphate (dCMP). Using this analysis, the background level of 8-OH-Gua in DNA from human lung carcinoma cells (A549) was several-fold lower than that obtained by a previous method. A549 cells were exposed to 20-60 Gy of gamma-radiation and an increase in 8-OH-Gua concentration was observed with increasing gamma-ray dose (0.3 residues per 10(7) dCMP per Gy). Moreover, by an immunohistochemical procedure using a commercial FITC-kit, 8-OH-Gua was clearly detected in A549 cells and the fluorescence intensity of cells with oxidative DNA damage increased with the doses of gamma-irradiation. Using an endonuclease nicking assay, we also found that gamma-rays decreased 8-OH-Gua repair activity. The results indicate that 8-OH-dGMP is a useful and sensitive marker for estimating oxidative damage in DNA. Topics: Biomarkers; Carcinoma; DNA Damage; DNA Repair; Epithelial Cells; Gamma Rays; Guanine; Guanosine Monophosphate; Humans; Lung Neoplasms; Oxidative Stress; Toxicity Tests; Tumor Cells, Cultured	2003

Article

Year

Analysis of 8-hydroxydeoxyguanosine 5'-monophosphate (8-OH-dGMP) as a reliable marker of cellular oxidative DNA damage after gamma-irradiation.

Environmental and molecular mutagenesis, 2003, Volume: 41, Issue:5

In order to improve 8-hydroxyguanine (8-OH-Gua) detection in DNA, we digested isolated DNA with nuclease P1 and analyzed for 8-hydroxydeoxyguanosine 5'-monophosphate (8-OH-dGMP) using a high-performance liquid chromatography system equipped with an electrochemical detector (HPLC-ECD). The amount of 8-OH-Gua in the DNA was expressed as the ratio of 8-OH-dGMP to deoxycytidine monophosphate (dCMP). Using this analysis, the background level of 8-OH-Gua in DNA from human lung carcinoma cells (A549) was several-fold lower than that obtained by a previous method. A549 cells were exposed to 20-60 Gy of gamma-radiation and an increase in 8-OH-Gua concentration was observed with increasing gamma-ray dose (0.3 residues per 10(7) dCMP per Gy). Moreover, by an immunohistochemical procedure using a commercial FITC-kit, 8-OH-Gua was clearly detected in A549 cells and the fluorescence intensity of cells with oxidative DNA damage increased with the doses of gamma-irradiation. Using an endonuclease nicking assay, we also found that gamma-rays decreased 8-OH-Gua repair activity. The results indicate that 8-OH-dGMP is a useful and sensitive marker for estimating oxidative damage in DNA.

Topics: Biomarkers; Carcinoma; DNA Damage; DNA Repair; Epithelial Cells; Gamma Rays; Guanine; Guanosine Monophosphate; Humans; Lung Neoplasms; Oxidative Stress; Toxicity Tests; Tumor Cells, Cultured

2003