8-bromocyclic-gmp and inositol-1-3-4-5-tetrakisphosphate

8-bromocyclic-gmp has been researched along with inositol-1-3-4-5-tetrakisphosphate* in 1 studies

Other Studies

1 other study(ies) available for 8-bromocyclic-gmp and inositol-1-3-4-5-tetrakisphosphate

ArticleYear
Cyclic GMP modulates depletion-activated Ca2+ entry in pancreatic acinar cells.
    The Journal of biological chemistry, 1993, May-25, Volume: 268, Issue:15

    In the pancreatic acinar cell, hormonal stimulation causes a rise in the intracellular free Ca2+ concentration by activating the inositol 1,4,5-trisphosphate-mediated release of Ca2+ from intracellular stores (Berridge, M. J., and Irvine, R. F. (1989) Nature 341, 197-205). The released Ca2+ is, for the most part, extruded from the cell, necessitating a mechanism for Ca2+ entry and reloading of intracellular Ca2+ stores (Putney, J. W., Jr. (1990) Cell Calcium 11, 611-624; Rink, T. J. (1990) FEBS Lett. 268, 381-385). However, neither the mechanism of depletion-activated Ca2+ entry nor the signal that activates it is known. We report here that a sustained inward current of depletion-activated Ca2+ entry can be measured in pancreatic acinar cells using patch-clamp recording methods. Furthermore, the current can be blocked by an inhibitor of guanylyl cyclase, can be reactivated by 8-bromo-cGMP after inhibition, and can be activated in the absence of Ca2+ depletion by perfusing the cell with cGMP, but not cAMP. Intracellular perfusion with 1,3,4,5-inositol tetrakisphosphate did not activate an inward current, whereas perfusion with 2,4,5-inositol trisphosphate did activate an inward current. We conclude that cGMP may be an intracellular messenger that regulates depletion-activated Ca2+ entry.

    Topics: Aminoquinolines; Animals; Calcium; Calcium Channels; Cyclic AMP; Cyclic GMP; Guanylate Cyclase; In Vitro Techniques; Inositol 1,4,5-Trisphosphate; Inositol Phosphates; Kinetics; Membrane Potentials; Models, Biological; Pancreas; Rats; Rats, Sprague-Dawley; Second Messenger Systems; Time Factors

1993