8-azidoadenine and phenylnitrene

8-azidoadenine has been researched along with phenylnitrene* in 1 studies

Other Studies

1 other study(ies) available for 8-azidoadenine and phenylnitrene

Article	Year
Ultraviolet crosslinking of DNA protein complexes via 8-azidoadenine. Methods in molecular biology (Clifton, N.J.), 2009, Volume: 543 The synthesis of 8-azido-2'-deoxyadenosine-5'-triphosphate is described. The photoreactive dATP analog was characterized by thin layer chromatography and UV spectroscopy. Its photoreactivity upon UV irradiation was studied. After incorporation of this dATP analog by nick translation into DNA containing the tet operator sequence the investigation of the interactions between tet operator DNA and Tet repressor becomes possible. Photocrosslinking of protein to DNA was demonstrated by the reduced migration of the DNA protein crosslinks in SDS polyacrylamide gel electrophoresis. Topics: Adenine; Adenine Nucleotides; Azides; Chromatography, Ion Exchange; Cross-Linking Reagents; Deoxyadenine Nucleotides; DNA; DNA Adducts; Imines; Molecular Biology; Molecular Conformation; Photoaffinity Labels; Plasmids; Proteins; Spectrum Analysis; Ultraviolet Rays	2009

Article

Year

Ultraviolet crosslinking of DNA protein complexes via 8-azidoadenine.

Methods in molecular biology (Clifton, N.J.), 2009, Volume: 543

The synthesis of 8-azido-2'-deoxyadenosine-5'-triphosphate is described. The photoreactive dATP analog was characterized by thin layer chromatography and UV spectroscopy. Its photoreactivity upon UV irradiation was studied. After incorporation of this dATP analog by nick translation into DNA containing the tet operator sequence the investigation of the interactions between tet operator DNA and Tet repressor becomes possible. Photocrosslinking of protein to DNA was demonstrated by the reduced migration of the DNA protein crosslinks in SDS polyacrylamide gel electrophoresis.

Topics: Adenine; Adenine Nucleotides; Azides; Chromatography, Ion Exchange; Cross-Linking Reagents; Deoxyadenine Nucleotides; DNA; DNA Adducts; Imines; Molecular Biology; Molecular Conformation; Photoaffinity Labels; Plasmids; Proteins; Spectrum Analysis; Ultraviolet Rays

2009