7-methylguanosine and 7-ketopyrazolo(4-3-d)pyrimidine

7-methylguanosine has been researched along with 7-ketopyrazolo(4-3-d)pyrimidine* in 1 studies

Other Studies

1 other study(ies) available for 7-methylguanosine and 7-ketopyrazolo(4-3-d)pyrimidine

Article	Year
Interactions of calf spleen purine nucleoside phosphorylase with formycin B and its aglycone - spectroscopic and kinetic studies. Nucleosides, nucleotides & nucleic acids, 2007, Volume: 26, Issue:6-7 Phosphorolysis of 7-methylguanosine by calf spleen purine nucleoside phosphorylase (PNP) is weakly inhibited, uncompetitively, by Formycin B (FB) with Ki = 100 micro M and more effectively by its aglycone (7KPP), IC50 35-100 micro M. In striking contrast, 7KPP inhibits the reverse reaction (synthesis of 8-azaguanosine from 8-azaguanine) competitively, with Ki approximately 2-4 micro M. Formycin B forms only a weakly fluorescent complex with PNP, and 7KPP even less so, indicating that both ligands bind as the neutral, not anionic, forms. 7KPP is a rare example of a PNP non-substrate inhibitor of both the phosphorolytic and reverse synthetic pathways. Topics: Animals; Cattle; Formycins; Guanosine; Kinetics; Purine-Nucleoside Phosphorylase; Pyrimidines; Spectrometry, Fluorescence; Spleen; Substrate Specificity	2007

Article

Year

Interactions of calf spleen purine nucleoside phosphorylase with formycin B and its aglycone - spectroscopic and kinetic studies.

Nucleosides, nucleotides & nucleic acids, 2007, Volume: 26, Issue:6-7

Phosphorolysis of 7-methylguanosine by calf spleen purine nucleoside phosphorylase (PNP) is weakly inhibited, uncompetitively, by Formycin B (FB) with Ki = 100 micro M and more effectively by its aglycone (7KPP), IC50 35-100 micro M. In striking contrast, 7KPP inhibits the reverse reaction (synthesis of 8-azaguanosine from 8-azaguanine) competitively, with Ki approximately 2-4 micro M. Formycin B forms only a weakly fluorescent complex with PNP, and 7KPP even less so, indicating that both ligands bind as the neutral, not anionic, forms. 7KPP is a rare example of a PNP non-substrate inhibitor of both the phosphorolytic and reverse synthetic pathways.

Topics: Animals; Cattle; Formycins; Guanosine; Kinetics; Purine-Nucleoside Phosphorylase; Pyrimidines; Spectrometry, Fluorescence; Spleen; Substrate Specificity

2007