7-deazapurine and triphosphoric-acid

7-deazapurine has been researched along with triphosphoric-acid* in 2 studies

Other Studies

2 other study(ies) available for 7-deazapurine and triphosphoric-acid

Article	Year
Brightly Fluorescent 2'-Deoxyribonucleoside Triphosphates Bearing Methylated Bodipy Fluorophore for in Cellulo Incorporation to DNA, Imaging, and Flow Cytometry. Bioconjugate chemistry, 2018, 11-21, Volume: 29, Issue:11 Synthesis of cytosine, uracil, and 7-deazaadenine 2'-deoxyribonucleosides and triphosphates (dNTPs) bearing hexamethylated phenyl-bodipy fluorophore attached at position 5 of pyrimidines or at position 7 of 7-deazapurine was developed. All the title labeled nucleosides and dNTPs displayed bright green fluorescence with very high quantum yields. The modified dN Topics: Adenine; Boron Compounds; Cell Line, Tumor; Cytosine; Deoxyribonucleosides; DNA; Flow Cytometry; Fluorescent Dyes; Humans; Methylation; Optical Imaging; Polyphosphates; Purines; Staining and Labeling	2018
Structures of KlenTaq DNA polymerase caught while incorporating C5-modified pyrimidine and C7-modified 7-deazapurine nucleoside triphosphates. Journal of the American Chemical Society, 2012, Jul-25, Volume: 134, Issue:29 The capability of DNA polymerases to accept chemically modified nucleotides is of paramount importance for many biotechnological applications. Although these analogues are widely used, the structural basis for the acceptance of the unnatural nucleotide surrogates has been only sparsely explored. Here we present in total six crystal structures of modified 2'-deoxynucleoside-5'-O-triphosphates (dNTPs) carrying modifications at the C5 positions of pyrimidines or C7 positions of 7-deazapurines in complex with a DNA polymerase and a primer/template complex. The modified dNTPs are in positions poised for catalysis leading to incorporation. These structural data provide insight into the mechanism of incorporation and acceptance of modified dNTPs. Our results open the door for rational design of modified nucleotides, which should offer great opportunities for future applications. Topics: Crystallography, X-Ray; DNA Polymerase I; Models, Molecular; Polyphosphates; Protein Binding; Protein Conformation; Purine Nucleosides; Purines; Pyrimidine Nucleosides; Thermus	2012

Article

Year

Brightly Fluorescent 2'-Deoxyribonucleoside Triphosphates Bearing Methylated Bodipy Fluorophore for in Cellulo Incorporation to DNA, Imaging, and Flow Cytometry.

Bioconjugate chemistry, 2018, 11-21, Volume: 29, Issue:11

Synthesis of cytosine, uracil, and 7-deazaadenine 2'-deoxyribonucleosides and triphosphates (dNTPs) bearing hexamethylated phenyl-bodipy fluorophore attached at position 5 of pyrimidines or at position 7 of 7-deazapurine was developed. All the title labeled nucleosides and dNTPs displayed bright green fluorescence with very high quantum yields. The modified dN

Topics: Adenine; Boron Compounds; Cell Line, Tumor; Cytosine; Deoxyribonucleosides; DNA; Flow Cytometry; Fluorescent Dyes; Humans; Methylation; Optical Imaging; Polyphosphates; Purines; Staining and Labeling

2018

Structures of KlenTaq DNA polymerase caught while incorporating C5-modified pyrimidine and C7-modified 7-deazapurine nucleoside triphosphates.

Journal of the American Chemical Society, 2012, Jul-25, Volume: 134, Issue:29

The capability of DNA polymerases to accept chemically modified nucleotides is of paramount importance for many biotechnological applications. Although these analogues are widely used, the structural basis for the acceptance of the unnatural nucleotide surrogates has been only sparsely explored. Here we present in total six crystal structures of modified 2'-deoxynucleoside-5'-O-triphosphates (dNTPs) carrying modifications at the C5 positions of pyrimidines or C7 positions of 7-deazapurines in complex with a DNA polymerase and a primer/template complex. The modified dNTPs are in positions poised for catalysis leading to incorporation. These structural data provide insight into the mechanism of incorporation and acceptance of modified dNTPs. Our results open the door for rational design of modified nucleotides, which should offer great opportunities for future applications.

Topics: Crystallography, X-Ray; DNA Polymerase I; Models, Molecular; Polyphosphates; Protein Binding; Protein Conformation; Purine Nucleosides; Purines; Pyrimidine Nucleosides; Thermus

2012